opsd_bovin
NAME
2PED
ORGANISM
Bovine (Bos taurus)
PROTEIN NAME
opsd_bovin
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
N
|
G
|
T
|
E
|
G
|
P
|
N
|
F
|
Y
|
10 |
V
|
P
|
F
|
S
|
N
|
K
|
T
|
G
|
V
|
V
|
20 |
R
|
S
|
P
|
F
|
E
|
A
|
P
|
Q
|
Y
|
Y
|
30 |
TM1 | |||||||||
L
|
A
|
E
|
P
|
W
|
Q
|
F
|
S
|
M
|
L
|
40 |
A
|
A
|
Y
|
M
|
F
|
L
|
L
|
I
|
M
|
L
|
50 |
G
|
F
|
P
|
I
|
N
|
F
|
L
|
T
|
L
|
Y
|
60 |
ICL1 | |||||||||
V
|
T
|
V
|
Q
|
H
|
K
|
K
|
L
|
R
|
T
|
70 |
TM2 | |||||||||
P
|
L
|
N
|
Y
|
I
|
L
|
L
|
N
|
L
|
A
|
80 |
V
|
A
|
D
|
L
|
F
|
M
|
V
|
F
|
G
|
G
|
90 |
F
|
T
|
T
|
T
|
L
|
Y
|
T
|
S
|
L
|
H
|
100 |
ECL1 | TM3 | ||||||||
G
|
Y
|
F
|
V
|
F
|
G
|
P
|
T
|
G
|
C
|
110 |
N
|
L
|
E
|
G
|
F
|
F
|
A
|
T
|
L
|
G
|
120 |
G
|
E
|
I
|
A
|
L
|
W
|
S
|
L
|
V
|
V
|
130 |
L
|
A
|
I
|
E
|
R
|
Y
|
V
|
V
|
V
|
C
|
140 |
ICL2 | |||||||||
K
|
P
|
M
|
S
|
N
|
F
|
R
|
F
|
G
|
E
|
150 |
TM4 | |||||||||
N
|
H
|
A
|
I
|
M
|
G
|
V
|
A
|
F
|
T
|
160 |
W
|
V
|
M
|
A
|
L
|
A
|
C
|
A
|
A
|
P
|
170 |
ECL2 | |||||||||
P
|
L
|
V
|
G
|
W
|
S
|
R
|
Y
|
I
|
P
|
180 |
E
|
G
|
M
|
Q
|
C
|
S
|
C
|
G
|
I
|
D
|
190 |
Y
|
Y
|
T
|
P
|
H
|
E
|
E
|
T
|
N
|
N
|
200 |
TM5 | |||||||||
E
|
S
|
F
|
V
|
I
|
Y
|
M
|
F
|
V
|
V
|
210 |
H
|
F
|
I
|
I
|
P
|
L
|
I
|
V
|
I
|
F
|
220 |
F
|
C
|
Y
|
G
|
Q
|
L
|
V
|
F
|
T
|
V
|
230 |
K
|
E
|
A
|
A
|
A
|
Q
|
Q
|
Q
|
E
|
S
|
240 |
TM6 | |||||||||
A
|
T
|
T
|
Q
|
K
|
A
|
E
|
K
|
E
|
V
|
250 |
T
|
R
|
M
|
V
|
I
|
I
|
M
|
V
|
I
|
A
|
260 |
F
|
L
|
I
|
C
|
W
|
L
|
P
|
Y
|
A
|
G
|
270 |
V
|
A
|
F
|
Y
|
I
|
F
|
T
|
H
|
Q
|
G
|
280 |
ECL3 | TM7 | ||||||||
S
|
D
|
F
|
G
|
P
|
I
|
F
|
M
|
T
|
I
|
290 |
P
|
A
|
F
|
F
|
A
|
K
|
T
|
S
|
A
|
V
|
300 |
Y
|
N
|
P
|
V
|
I
|
Y
|
I
|
M
|
M
|
N
|
310 |
H8 | |||||||||
K
|
Q
|
F
|
R
|
N
|
C
|
M
|
V
|
T
|
T
|
320 |
C-term | |||||||||
L
|
C
|
C
|
G
|
K
|
N
|
P
|
L
|
G
|
D
|
330 |
D
|
E
|
A
|
S
|
T
|
T
|
V
|
S
|
K
|
T
|
340 |
E
|
T
|
S
|
Q
|
V
|
A
|
P
|
A
|
N-term | |||||||||
M
|
N
|
G
|
T
|
E
|
G
|
P
|
N
|
F
|
Y
|
10 |
V
|
P
|
F
|
S
|
N
|
K
|
T
|
G
|
V
|
V
|
20 |
R
|
S
|
P
|
F
|
E
|
A
|
P
|
Q
|
Y
|
Y
|
30 |
TM1 | |||||||||
L
|
A
|
E
|
P
|
W
|
Q
|
F
|
S
|
M
|
L
|
40 |
A
|
A
|
Y
|
M
|
F
|
L
|
L
|
I
|
M
|
L
|
50 |
G
|
F
|
P
|
I
|
N
|
F
|
L
|
T
|
L
|
Y
|
60 |
ICL1 | |||||||||
V
|
T
|
V
|
Q
|
H
|
K
|
K
|
L
|
R
|
T
|
70 |
TM2 | |||||||||
P
|
L
|
N
|
Y
|
I
|
L
|
L
|
N
|
L
|
A
|
80 |
V
|
A
|
D
|
L
|
F
|
M
|
V
|
F
|
G
|
G
|
90 |
F
|
T
|
T
|
T
|
L
|
Y
|
T
|
S
|
L
|
H
|
100 |
ECL1 | TM3 | ||||||||
G
|
Y
|
F
|
V
|
F
|
G
|
P
|
T
|
G
|
C
|
110 |
N
|
L
|
E
|
G
|
F
|
F
|
A
|
T
|
L
|
G
|
120 |
G
|
E
|
I
|
A
|
L
|
W
|
S
|
L
|
V
|
V
|
130 |
L
|
A
|
I
|
E
|
R
|
Y
|
V
|
V
|
V
|
C
|
140 |
ICL2 | |||||||||
K
|
P
|
M
|
S
|
N
|
F
|
R
|
F
|
G
|
E
|
150 |
TM4 | |||||||||
N
|
H
|
A
|
I
|
M
|
G
|
V
|
A
|
F
|
T
|
160 |
W
|
V
|
M
|
A
|
L
|
A
|
C
|
A
|
A
|
P
|
170 |
ECL2 | |||||||||
P
|
L
|
V
|
G
|
W
|
S
|
R
|
Y
|
I
|
P
|
180 |
E
|
G
|
M
|
Q
|
C
|
S
|
C
|
G
|
I
|
D
|
190 |
Y
|
Y
|
T
|
P
|
H
|
E
|
E
|
T
|
N
|
N
|
200 |
TM5 | |||||||||
E
|
S
|
F
|
V
|
I
|
Y
|
M
|
F
|
V
|
V
|
210 |
H
|
F
|
I
|
I
|
P
|
L
|
I
|
V
|
I
|
F
|
220 |
F
|
C
|
Y
|
G
|
Q
|
L
|
V
|
F
|
T
|
V
|
230 |
K
|
E
|
A
|
A
|
A
|
Q
|
Q
|
Q
|
E
|
S
|
240 |
TM6 | |||||||||
A
|
T
|
T
|
Q
|
K
|
A
|
E
|
K
|
E
|
V
|
250 |
T
|
R
|
M
|
V
|
I
|
I
|
M
|
V
|
I
|
A
|
260 |
F
|
L
|
I
|
C
|
W
|
L
|
P
|
Y
|
A
|
G
|
270 |
V
|
A
|
F
|
Y
|
I
|
F
|
T
|
H
|
Q
|
G
|
280 |
ECL3 | TM7 | ||||||||
S
|
D
|
F
|
G
|
P
|
I
|
F
|
M
|
T
|
I
|
290 |
P
|
A
|
F
|
F
|
A
|
K
|
T
|
S
|
A
|
V
|
300 |
Y
|
N
|
P
|
V
|
I
|
Y
|
I
|
M
|
M
|
N
|
310 |
H8 | |||||||||
K
|
Q
|
F
|
R
|
N
|
C
|
M
|
V
|
T
|
T
|
320 |
C-term | |||||||||
L
|
C
|
C
|
G
|
K
|
N
|
P
|
L
|
G
|
D
|
330 |
D
|
E
|
A
|
S
|
T
|
T
|
V
|
S
|
K
|
T
|
340 |
E
|
T
|
S
|
Q
|
V
|
A
|
P
|
A
|
348 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
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|
|
|
|
|
|
|
|
|
|
|
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
NA | Native | Bovine |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
additive | HPTO(heptylthioglucoside) | 1.1-1.3 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
NONE | None |
Remarks:None
CRYSTALLIZATION
METHOD: vapour diffusion
TYPE: Hanging drop ()
PROTEIN CONCENTRATION: 3.5 mg/ml
Temperature: 283 K
ph : 6.4-6.4
Remarks:
Chemical lists
Type | Item | Concentration |
---|---|---|
Additional | ||
unknown | Ammonium sulfate | 2.8-3.1 M |
unknown | MES pH 5.9-6.2 | 20 mM |
zinc acetate | 70 mM | |
2-mercaptoethanol | 5 mM | |
Detergent | ||
HTG(heptylthioglucoside) | 1.4-1.6 %w/v |