adrb1_melga
NAME
2VT4
ORGANISM
Wild turkey (Meleagris gallopavo)
PROTEIN NAME
adrb1_melga
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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G
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D
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G
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W
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L
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P
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P
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D
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C
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10 |
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G
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P
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H
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N
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R
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S
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G
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G
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G
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G
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20 |
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A
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T
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A
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A
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P
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T
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G
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S
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R
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Q
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30 |
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||||||||
TM1 | |||||||||
V
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S
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A
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E
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L
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L
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S
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Q
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Q
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W
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40 |
E
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A
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G
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M
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S
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L
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L
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M
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A
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L
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50 |
V
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V
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L
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L
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I
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V
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A
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G
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N
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V
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60 |
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|||||||||
L
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V
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I
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A
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A
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I
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G
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R
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T
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Q
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70 |
ICL1 | TM2 | ||||||||
R
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L
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Q
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T
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L
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T
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N
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L
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F
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I
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80 |
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|||||||||
T
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S
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L
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A
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C
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A
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D
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L
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V
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M
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90 |
G
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L
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L
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V
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V
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P
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F
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G
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A
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T
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100 |
ECL1 | |||||||||
L
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V
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V
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R
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G
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T
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W
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L
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W
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G
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110 |
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|||||||||
TM3 | |||||||||
S
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F
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L
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C
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E
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C
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W
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T
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S
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L
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120 |
D
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V
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L
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C
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V
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T
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A
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S
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I
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E
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130 |
T
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L
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C
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V
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I
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A
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I
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D
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R
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Y
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140 |
ICL2 | |||||||||
L
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A
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I
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T
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S
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P
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F
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R
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Y
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Q
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150 |
TM4 | |||||||||
S
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L
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M
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T
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R
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A
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R
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A
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K
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V
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160 |
I
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I
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C
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T
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V
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W
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A
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I
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S
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A
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170 |
L
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V
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S
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F
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L
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P
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I
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M
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M
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H
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180 |
ECL2 | |||||||||
W
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W
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R
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D
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E
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D
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P
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Q
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A
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L
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190 |
K
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C
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Y
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Q
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D
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P
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G
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C
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C
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D
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200 |
TM5 | |||||||||
F
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V
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T
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N
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R
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A
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Y
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A
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I
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A
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210 |
S
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S
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I
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I
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S
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F
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Y
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I
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P
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L
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220 |
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|||||||||
L
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I
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M
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I
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F
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V
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Y
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L
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R
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V
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230 |
Y
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R
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E
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A
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K
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E
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Q
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I
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R
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K
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240 |
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ICL3 | |||||||||
I
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D
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R
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C
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E
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G
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R
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F
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Y
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G
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250 |
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S
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Q
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E
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Q
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P
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Q
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P
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P
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P
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L
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260 |
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P
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Q
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H
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Q
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P
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I
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L
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G
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N
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G
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270 |
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TM6 | |||||||||
R
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A
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S
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K
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R
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K
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T
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S
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R
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V
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280 |
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|||||||||
M
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A
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M
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R
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E
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H
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K
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A
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L
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K
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290 |
T
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L
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G
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I
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I
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M
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G
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V
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F
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T
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300 |
L
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C
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W
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L
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P
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F
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F
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L
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V
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N
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310 |
ECL3 | |||||||||
I
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V
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N
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V
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F
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N
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R
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D
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L
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V
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320 |
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TM7 | |||||||||
P
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D
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W
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L
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F
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V
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F
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F
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N
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W
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330 |
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|||||||||
L
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G
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Y
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A
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N
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S
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A
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F
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N
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P
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340 |
H8 | |||||||||
I
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I
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Y
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C
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R
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S
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P
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D
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F
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R
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350 |
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|||||||||
K
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A
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F
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K
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R
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L
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L
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C
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F
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P
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360 |
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C-term | |||||||||
R
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K
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A
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D
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R
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R
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L
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H
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A
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G
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370 |
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G
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Q
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P
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A
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P
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L
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P
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G
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G
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F
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380 |
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I
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S
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T
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L
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G
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S
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P
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E
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H
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S
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390 |
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P
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G
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G
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T
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W
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S
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D
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C
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N
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G
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400 |
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G
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T
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R
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G
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G
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S
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E
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S
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S
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L
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410 |
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E
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E
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R
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H
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S
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K
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T
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S
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R
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S
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420 |
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E
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S
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K
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M
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E
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R
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E
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K
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N
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I
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430 |
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L
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A
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T
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T
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R
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F
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Y
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C
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T
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F
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440 |
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L
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G
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N
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G
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D
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K
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A
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V
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F
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C
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450 |
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T
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V
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L
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R
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I
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V
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K
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L
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F
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E
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460 |
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D
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A
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T
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C
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T
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C
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P
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H
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T
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H
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470 |
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K
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L
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K
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M
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K
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W
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R
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F
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K
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Q
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480 |
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H
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Q
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A
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|||||||||
TM1 | |||||||||
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A
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E
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L
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L
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S
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Q
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Q
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W
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E
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41 |
A
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G
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M
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S
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L
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L
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M
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A
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L
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V
|
51 |
V
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L
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L
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I
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V
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A
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G
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N
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V
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L
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61 |
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|||||||||
V
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I
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A
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A
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I
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G
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S
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T
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Q
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R
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71 |
ICL1 | TM2 | ||||||||
L
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Q
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T
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L
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T
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N
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L
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F
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I
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T
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81 |
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|||||||||
S
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L
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A
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C
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A
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D
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L
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V
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V
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G
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91 |
L
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L
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V
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V
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P
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F
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G
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A
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T
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L
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101 |
ECL1 | |||||||||
V
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V
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R
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G
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T
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W
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L
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W
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G
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S
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111 |
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|||||||||
TM3 | |||||||||
F
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L
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C
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E
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L
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W
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T
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S
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L
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D
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121 |
V
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L
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C
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V
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T
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A
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S
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I
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E
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T
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131 |
L
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C
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V
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I
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A
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I
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D
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R
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Y
|
L
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141 |
ICL2 | |||||||||
A
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I
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T
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S
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P
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F
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R
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Y
|
Q
|
S
|
151 |
TM4 | |||||||||
L
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M
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T
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R
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A
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R
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A
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K
|
V
|
I
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161 |
I
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C
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T
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V
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W
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A
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I
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S
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A
|
L
|
171 |
V
|
S
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F
|
L
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P
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I
|
M
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M
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H
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W
|
181 |
ECL2 | |||||||||
W
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R
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D
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E
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D
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P
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Q
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A
|
L
|
K
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191 |
C
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Y
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Q
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D
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P
|
G
|
C
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C
|
D
|
F
|
201 |
TM5 | |||||||||
V
|
T
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N
|
R
|
A
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Y
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A
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I
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A
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S
|
211 |
S
|
I
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I
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S
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F
|
Y
|
I
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P
|
L
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L
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221 |
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|||||||||
I
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M
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I
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F
|
V
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A
|
L
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R
|
V
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Y
|
231 |
R
|
E
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A
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K
|
E
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Q
|
I
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R
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K
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I
|
241 |
|
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||||||||
ICL3 | TM6 | ||||||||
D
|
R
|
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A
|
S
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K
|
R
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K
|
|
R
|
279 |
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|||||||||
V
|
M
|
L
|
M
|
R
|
E
|
H
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K
|
A
|
L
|
289 |
K
|
T
|
L
|
G
|
I
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I
|
M
|
G
|
V
|
F
|
299 |
T
|
L
|
C
|
W
|
L
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P
|
F
|
F
|
L
|
V
|
309 |
N
|
I
|
V
|
N
|
V
|
F
|
N
|
R
|
D
|
L
|
319 |
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|||||||||
ECL3 | TM7 | ||||||||
V
|
P
|
D
|
W
|
L
|
F
|
V
|
A
|
F
|
N
|
329 |
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|||||||||
W
|
L
|
G
|
Y
|
A
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N
|
S
|
A
|
M
|
N
|
339 |
H8 | |||||||||
P
|
I
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I
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Y
|
C
|
R
|
S
|
P
|
D
|
F
|
349 |
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|||||||||
R
|
K
|
A
|
F
|
K
|
R
|
L
|
L
|
A
|
F
|
359 |
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||||||||
C-term | ||||||||
P
|
R
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K
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A
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D
|
R
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R
|
L
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|
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His(6) | |||||||||
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
68 | | R | S |
90 | | M | V |
116 | | C | L |
227 | | Y | A |
282 | | A | L |
327 | | F | A |
338 | | F | M |
358 | | C | A |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: tag
Name: His(6)
Sequence: None
Deletions:
Positions: C-term_0 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Hi5 | Insect | pBacPAK8 co-transfection linearized baculovirus DNA (Baculogold) |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DM | 2.0 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Detergent DM exchanged with 0.1% OG (octylthioglucoside) | None |
Alkaline phosphatase | None |
Remarks:None
CRYSTALLIZATION
METHOD: vapour diffusion
TYPE: hanging drop ()
PROTEIN CONCENTRATION: 6 mg/ml
Temperature: 18°C
ph : 6.9-7.3
Remarks:
Chemical lists
Type | Item | Concentration |
---|---|---|
Additional | ||
unknown | ADA:NaOH pH 6.9-7.3 | 0.1 M |
unknown | Tris/HCl pH 7.7 | 20 mM |
unknown | PEG600 | 29-32 %v/v |
Detergent | ||
detergent | OTG (OCTYLTHIOGLUCOSIDE) | 0.35 %v/v |