aa2ar_human
NAME
3EML
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
aa2ar_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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TM1 | |||||||||
M
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P
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I
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M
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G
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S
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S
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V
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Y
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I
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10 |
T
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V
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E
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L
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A
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I
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A
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V
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L
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A
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20 |
I
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L
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G
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N
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V
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L
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V
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C
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W
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A
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30 |
ICL1 | |||||||||
V
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W
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L
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N
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S
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N
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L
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Q
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N
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V
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40 |
TM2 | |||||||||
T
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N
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Y
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F
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V
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V
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S
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L
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A
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A
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50 |
A
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D
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I
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A
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V
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G
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V
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L
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A
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I
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60 |
P
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F
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A
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I
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T
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I
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S
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T
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G
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F
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70 |
ECL1 | TM3 | ||||||||
C
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A
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A
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C
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H
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G
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C
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L
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F
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I
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80 |
A
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C
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F
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V
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L
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V
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L
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T
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Q
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S
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90 |
S
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I
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F
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S
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L
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L
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A
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I
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A
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I
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100 |
D
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R
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Y
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I
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A
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I
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R
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I
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P
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L
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110 |
ICL2 | TM4 | ||||||||
R
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Y
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N
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G
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L
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V
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T
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G
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T
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R
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120 |
A
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K
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G
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I
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I
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A
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I
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C
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W
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V
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130 |
L
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S
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F
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A
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I
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G
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L
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T
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P
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M
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140 |
ECL2 | |||||||||
L
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G
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W
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N
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N
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C
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G
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Q
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P
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K
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150 |
E
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G
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K
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N
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H
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S
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Q
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G
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C
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G
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160 |
E
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G
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Q
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V
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A
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C
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L
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F
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E
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D
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170 |
TM5 | |||||||||
V
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V
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P
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M
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N
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Y
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M
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V
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Y
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F
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180 |
N
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F
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F
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A
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C
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V
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L
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V
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P
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L
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190 |
L
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L
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M
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L
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G
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V
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Y
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L
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R
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I
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200 |
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F
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L
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A
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A
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R
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R
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Q
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L
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K
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Q
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210 |
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ICL3 | |||||||||
M
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E
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S
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Q
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P
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L
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P
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G
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E
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R
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220 |
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TM6 | |||||||||
A
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R
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S
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T
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L
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Q
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K
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E
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V
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H
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230 |
A
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A
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K
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S
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L
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A
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I
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I
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V
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G
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240 |
L
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F
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A
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L
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C
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W
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L
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P
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L
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H
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250 |
I
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I
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N
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C
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F
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T
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F
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F
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C
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P
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260 |
ECL3 | TM7 | ||||||||
D
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C
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S
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H
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A
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P
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L
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W
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L
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M
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270 |
Y
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L
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A
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I
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V
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L
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S
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H
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T
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N
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280 |
S
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V
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V
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N
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P
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F
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I
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Y
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A
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Y
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290 |
H8 | |||||||||
R
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I
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R
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E
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F
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R
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Q
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T
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F
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R
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300 |
K
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I
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I
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R
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S
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H
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V
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L
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R
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Q
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310 |
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C-term | |||||||||
Q
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E
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P
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F
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K
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A
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A
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G
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T
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S
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320 |
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A
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R
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V
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L
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A
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A
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H
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G
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S
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D
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330 |
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G
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E
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Q
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V
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S
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L
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R
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L
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N
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G
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340 |
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H
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P
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P
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G
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V
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W
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A
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N
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G
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S
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350 |
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A
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P
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H
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P
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E
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R
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R
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P
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N
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G
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360 |
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Y
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A
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L
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G
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L
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V
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S
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G
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G
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S
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370 |
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A
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Q
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E
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S
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Q
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G
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N
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T
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G
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L
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380 |
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P
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D
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V
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E
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L
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L
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S
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H
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E
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L
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390 |
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K
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G
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V
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C
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P
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E
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P
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P
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G
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L
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400 |
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D
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D
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P
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L
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A
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Q
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D
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G
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A
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G
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410 |
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V
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S
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hemagglutin | ||||||||||
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FLAG | |||||||||
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PreScission | ||||||||||
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TM1 | |||||||||
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P
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I
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M
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G
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S
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S
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V
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Y
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I
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10 |
T
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V
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E
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L
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A
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I
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A
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V
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L
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A
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20 |
I
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L
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G
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N
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V
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L
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V
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C
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W
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A
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30 |
ICL1 | |||||||||
V
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W
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L
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N
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S
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N
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L
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Q
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N
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V
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40 |
TM2 | |||||||||
T
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N
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Y
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F
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V
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V
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S
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L
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A
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A
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50 |
A
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D
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I
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A
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V
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G
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V
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L
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A
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I
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60 |
P
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F
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A
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I
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T
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I
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S
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T
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G
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F
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70 |
ECL1 | TM3 | ||||||||
C
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A
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A
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C
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H
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G
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C
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L
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F
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I
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80 |
A
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C
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F
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V
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L
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V
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L
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T
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Q
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S
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90 |
S
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I
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F
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S
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L
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L
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A
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I
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A
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I
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100 |
D
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R
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Y
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I
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A
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I
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R
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I
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P
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L
|
110 |
ICL2 | TM4 | ||||||||
R
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Y
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N
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G
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L
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V
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T
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G
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T
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R
|
120 |
A
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K
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G
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I
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I
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A
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I
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C
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W
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V
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130 |
L
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S
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F
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A
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I
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G
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L
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T
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P
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M
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140 |
ECL2 | |||||||||
L
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G
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W
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N
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N
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C
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G
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Q
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P
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K
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150 |
E
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G
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K
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N
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H
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S
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Q
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G
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C
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G
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160 |
E
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G
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Q
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V
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A
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C
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L
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F
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E
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D
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170 |
TM5 | |||||||||
V
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V
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P
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M
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N
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Y
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M
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V
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Y
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F
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180 |
N
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F
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F
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A
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C
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V
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L
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V
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P
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L
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190 |
L
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L
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M
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L
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G
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V
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Y
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L
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R
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I
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200 |
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||||||||
F
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L
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A
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A
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R
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R
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Q
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L
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T4 Lysozyme | ||||||||||
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TM6 | |||||||
R
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S
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T
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L
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Q
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K
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E
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V
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229 |
H
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A
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A
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K
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S
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L
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A
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I
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I
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V
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239 |
G
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L
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F
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A
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L
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C
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W
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L
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P
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L
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249 |
H
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I
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I
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N
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C
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F
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T
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F
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F
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C
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259 |
ECL3 | TM7 | ||||||||
P
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D
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C
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S
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H
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A
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P
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L
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W
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L
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269 |
M
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Y
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L
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A
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I
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V
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L
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S
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H
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T
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279 |
N
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S
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V
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V
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N
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P
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F
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I
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Y
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A
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289 |
H8 | |||||||||
Y
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R
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I
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R
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E
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F
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R
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Q
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T
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F
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299 |
R
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K
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I
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I
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R
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S
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H
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V
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L
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R
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309 |
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|||||||
Q
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Q
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E
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P
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F
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K
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A
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His(10) | |||||||||
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: signal
Name: hemagglutinin
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: tag
Name: FLAG
Sequence: None
Deletions:
Positions: N-term_1 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: prot_cleavage
Name: PreScission site (3C) [paper]
Sequence: None
Deletions:
Positions: N-term_2 None:None
Presence in Crystal:
Remarks: None
Protein_type: fusion
Name: T4 Lysozyme (T4L)
Sequence: None
Deletions:
Positions: Within Receptor_3 209:221
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: His(10)
Sequence: None
Deletions:
Positions: C-term_4 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect | transfer plasmid pBac5 for Sapphireâ„¢ baculovirus DNA (Orbigen) |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
additive | CHS | 0.01 %w/v |
detergent | DDM | 0.5 % |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Alkylation by Iodoacetamide | None |
Deglycosylation by PNGase F | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 3-70 mg/ml
Temperature: 20°C
ph : 6.5-6.5
Remarks: Lipid:receptor LCP mixture typically contained monoolein:cholesterol(54%:6% (w/w)) and receptor (40% (w/w))
Chemical lists
Type | Item | Concentration |
---|---|---|
Additional | ||
unknown | PEG 400 | 30 % v/v |
unknown | Lithium sulfate | 186 mM |
unknown | Sodium citrate (pH 6.5) | 100 mM |
Detergent | ||
detergent | DDM | 0.05 % w/v |
Lipid | ||
lipid | CHS | 0.001 %w/v |