adrb2_human
NAME
3NYA
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
adrb2_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
G
|
Q
|
P
|
G
|
N
|
G
|
S
|
A
|
F
|
10 |
|
|||||||||
L
|
L
|
A
|
P
|
N
|
G
|
S
|
H
|
A
|
P
|
20 |
|
|||||||||
TM1 | |||||||||
D
|
H
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D
|
V
|
T
|
Q
|
E
|
R
|
D
|
E
|
30 |
V
|
W
|
V
|
V
|
G
|
M
|
G
|
I
|
V
|
M
|
40 |
S
|
L
|
I
|
V
|
L
|
A
|
I
|
V
|
F
|
G
|
50 |
N
|
V
|
L
|
V
|
I
|
T
|
A
|
I
|
A
|
K
|
60 |
ICL1 | TM2 | ||||||||
F
|
E
|
R
|
L
|
Q
|
T
|
V
|
T
|
N
|
Y
|
70 |
F
|
I
|
T
|
S
|
L
|
A
|
C
|
A
|
D
|
L
|
80 |
V
|
M
|
G
|
L
|
A
|
V
|
V
|
P
|
F
|
G
|
90 |
A
|
A
|
H
|
I
|
L
|
M
|
K
|
M
|
W
|
T
|
100 |
ECL1 | TM3 | ||||||||
F
|
G
|
N
|
F
|
W
|
C
|
E
|
F
|
W
|
T
|
110 |
S
|
I
|
D
|
V
|
L
|
C
|
V
|
T
|
A
|
S
|
120 |
|
|||||||||
I
|
E
|
T
|
L
|
C
|
V
|
I
|
A
|
V
|
D
|
130 |
R
|
Y
|
F
|
A
|
I
|
T
|
S
|
P
|
F
|
K
|
140 |
ICL2 | TM4 | ||||||||
Y
|
Q
|
S
|
L
|
L
|
T
|
K
|
N
|
K
|
A
|
150 |
R
|
V
|
I
|
I
|
L
|
M
|
V
|
W
|
I
|
V
|
160 |
S
|
G
|
L
|
T
|
S
|
F
|
L
|
P
|
I
|
Q
|
170 |
ECL2 | |||||||||
M
|
H
|
W
|
Y
|
R
|
A
|
T
|
H
|
Q
|
E
|
180 |
|
|||||||||
A
|
I
|
N
|
C
|
Y
|
A
|
N
|
E
|
T
|
C
|
190 |
TM5 | |||||||||
C
|
D
|
F
|
F
|
T
|
N
|
Q
|
A
|
Y
|
A
|
200 |
I
|
A
|
S
|
S
|
I
|
V
|
S
|
F
|
Y
|
V
|
210 |
P
|
L
|
V
|
I
|
M
|
V
|
F
|
V
|
Y
|
S
|
220 |
R
|
V
|
F
|
Q
|
E
|
A
|
K
|
R
|
Q
|
L
|
230 |
|
|
|
|
|
|
|
|
|
|
Q
|
K
|
I
|
D
|
K
|
S
|
E
|
G
|
R
|
F
|
240 |
|
|
|
|
|
|
|
|
|
|
ICL3 | |||||||||
H
|
V
|
Q
|
N
|
L
|
S
|
Q
|
V
|
E
|
Q
|
250 |
|
|
|
|
|
|
|
|
|
|
D
|
G
|
R
|
T
|
G
|
H
|
G
|
L
|
R
|
R
|
260 |
|
|
||||||||
TM6 | |||||||||
S
|
S
|
K
|
F
|
C
|
L
|
K
|
E
|
H
|
K
|
270 |
A
|
L
|
K
|
T
|
L
|
G
|
I
|
I
|
M
|
G
|
280 |
T
|
F
|
T
|
L
|
C
|
W
|
L
|
P
|
F
|
F
|
290 |
I
|
V
|
N
|
I
|
V
|
H
|
V
|
I
|
Q
|
D
|
300 |
ECL3 | TM7 | ||||||||
N
|
L
|
I
|
R
|
K
|
E
|
V
|
Y
|
I
|
L
|
310 |
L
|
N
|
W
|
I
|
G
|
Y
|
V
|
N
|
S
|
G
|
320 |
H8 | |||||||||
F
|
N
|
P
|
L
|
I
|
Y
|
C
|
R
|
S
|
P
|
330 |
D
|
F
|
R
|
I
|
A
|
F
|
Q
|
E
|
L
|
L
|
340 |
|
|
||||||||
C-term | |||||||||
C
|
L
|
R
|
R
|
S
|
S
|
L
|
K
|
A
|
Y
|
350 |
|
|
|
|
|
|
|
|
|
|
G
|
N
|
G
|
Y
|
S
|
S
|
N
|
G
|
N
|
T
|
360 |
|
|
|
|
|
|
|
|
|
|
G
|
E
|
Q
|
S
|
G
|
Y
|
H
|
V
|
E
|
Q
|
370 |
|
|
|
|
|
|
|
|
|
|
E
|
K
|
E
|
N
|
K
|
L
|
L
|
C
|
E
|
D
|
380 |
|
|
|
|
|
|
|
|
|
|
L
|
P
|
G
|
T
|
E
|
D
|
F
|
V
|
G
|
H
|
390 |
|
|
|
|
|
|
|
|
|
|
Q
|
G
|
T
|
V
|
P
|
S
|
D
|
N
|
I
|
D
|
400 |
|
|
|
|
|
|
|
|
|
|
S
|
Q
|
G
|
R
|
N
|
C
|
S
|
T
|
N
|
D
|
410 |
|
|
|
S
|
L
|
L
|
|
|
|
|
|
|
|
|
|
|
FLAG | |||||||||
|
|
|
|
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|
|
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|
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N-term | |||||||||
M
|
G
|
Q
|
P
|
G
|
N
|
G
|
S
|
A
|
F
|
10 |
|
|||||||||
L
|
L
|
A
|
P
|
N
|
R
|
S
|
H
|
A
|
P
|
20 |
|
|||||||||
TM1 | |||||||||
D
|
H
|
D
|
V
|
T
|
Q
|
Q
|
R
|
D
|
E
|
30 |
V
|
W
|
V
|
V
|
G
|
M
|
G
|
I
|
V
|
M
|
40 |
S
|
L
|
I
|
V
|
L
|
A
|
I
|
V
|
F
|
G
|
50 |
N
|
V
|
L
|
V
|
I
|
T
|
A
|
I
|
A
|
K
|
60 |
ICL1 | TM2 | ||||||||
F
|
E
|
R
|
L
|
Q
|
T
|
V
|
T
|
N
|
Y
|
70 |
F
|
I
|
T
|
S
|
L
|
A
|
C
|
A
|
D
|
L
|
80 |
V
|
M
|
G
|
L
|
A
|
V
|
V
|
P
|
F
|
G
|
90 |
A
|
A
|
H
|
I
|
L
|
M
|
K
|
M
|
W
|
T
|
100 |
ECL1 | TM3 | ||||||||
F
|
G
|
N
|
F
|
W
|
C
|
E
|
F
|
W
|
T
|
110 |
S
|
I
|
D
|
V
|
L
|
C
|
V
|
T
|
A
|
S
|
120 |
|
|||||||||
I
|
W
|
T
|
L
|
C
|
V
|
I
|
A
|
V
|
D
|
130 |
R
|
Y
|
F
|
A
|
I
|
T
|
S
|
P
|
F
|
K
|
140 |
ICL2 | TM4 | ||||||||
Y
|
Q
|
S
|
L
|
L
|
T
|
K
|
N
|
K
|
A
|
150 |
R
|
V
|
I
|
I
|
L
|
M
|
V
|
W
|
I
|
V
|
160 |
S
|
G
|
L
|
T
|
S
|
F
|
L
|
P
|
I
|
Q
|
170 |
ECL2 | |||||||||
M
|
H
|
W
|
Y
|
R
|
A
|
T
|
H
|
Q
|
E
|
180 |
|
|||||||||
A
|
I
|
N
|
C
|
Y
|
A
|
E
|
E
|
T
|
C
|
190 |
TM5 | |||||||||
C
|
D
|
F
|
F
|
T
|
N
|
Q
|
A
|
Y
|
A
|
200 |
I
|
A
|
S
|
S
|
I
|
V
|
S
|
F
|
Y
|
V
|
210 |
P
|
L
|
V
|
I
|
M
|
V
|
F
|
V
|
Y
|
S
|
220 |
|
|||||||||||||||||||||||||||||||||||||||
TM6 | |||||||||||||||||||||||||||||||||||||||
R
|
V
|
F
|
Q
|
E
|
A
|
K
|
R
|
Q
|
L
|
|
K
|
F
|
C
|
L
|
K
|
E
|
H
|
K
|
A
|
||||||||||||||||||||
230 | 271 |
L
|
K
|
T
|
L
|
G
|
I
|
I
|
M
|
G
|
T
|
281 |
F
|
T
|
L
|
C
|
W
|
L
|
P
|
F
|
F
|
I
|
291 |
V
|
N
|
I
|
V
|
H
|
V
|
I
|
Q
|
D
|
N
|
301 |
ECL3 | TM7 | ||||||||
L
|
I
|
R
|
K
|
E
|
V
|
Y
|
I
|
L
|
L
|
311 |
N
|
W
|
I
|
G
|
Y
|
V
|
N
|
S
|
G
|
F
|
321 |
H8 | |||||||||
N
|
P
|
L
|
I
|
Y
|
C
|
R
|
S
|
P
|
D
|
331 |
F
|
R
|
I
|
A
|
F
|
Q
|
E
|
L
|
L
|
C
|
341 |
|
|||||||
C-term | |||||||
L
|
R
|
R
|
S
|
S
|
L
|
K
|
|
|
|
|
|
|
|
|
|
|
|
His(6) | |||||||||
|
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|
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|
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
16 | | G | R |
27 | | E | Q |
122 | | E | W |
187 | | N | E |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: tag
Name: FLAG
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: T4 Lysozyme (T4L)
Sequence: None
Deletions:
Positions: Within Receptor_1 233:260
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: His(6)
Sequence: None
Deletions:
Positions: C-term_2 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect | Bac-to-Bac |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 0.5 %w/v |
additive | CHS | 0.1 %(w/v) |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Alkylation by Iodoacetamide | None |
Deglycosylation by PNGase F | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 61 mg/ml
Temperature: 21-23°C
ph : 7.0-7.0
Remarks:
Chemical lists
Type | Item | Concentration |
---|---|---|
Additional | ||
unknown | Bis-Tris Propane pH 6.6-6.8 | 100 mM |
unknown | Na-tartrate | 120 mM |
unknown | 1,3 butanediol | 3 % |
unknown | PEG 400 | 25-30 % |
Detergent | ||
detergent | DDM | 0.05 %w/v |
Lipid | ||
lipid | CHS | 0.01 %w/v |