par1_human
NAME
3VW7
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
par1_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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G
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P
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R
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R
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L
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L
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L
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V
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A
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10 |
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A
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C
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F
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S
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L
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C
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G
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P
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L
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L
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20 |
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S
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A
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R
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T
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R
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A
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R
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R
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P
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E
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30 |
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S
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K
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A
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T
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N
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A
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T
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L
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D
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P
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40 |
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R
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S
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F
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L
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L
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R
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N
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P
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N
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D
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50 |
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K
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Y
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E
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P
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F
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W
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E
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D
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E
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E
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60 |
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K
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N
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E
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S
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G
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L
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T
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E
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Y
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R
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70 |
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L
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V
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S
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I
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N
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K
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S
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S
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P
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L
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80 |
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|||||
Q
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K
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Q
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L
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P
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A
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F
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I
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S
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E
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90 |
D
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A
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S
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G
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Y
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L
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T
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S
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S
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W
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100 |
TM1 | |||||||||
L
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T
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L
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F
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V
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P
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S
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V
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Y
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T
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110 |
G
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V
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F
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V
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V
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S
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L
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P
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L
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N
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120 |
I
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M
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A
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I
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V
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V
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F
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I
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L
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K
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130 |
ICL1 | TM2 | ||||||||
M
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K
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V
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K
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K
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P
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A
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V
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V
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Y
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140 |
M
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L
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H
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L
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A
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T
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A
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D
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V
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L
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150 |
F
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V
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S
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V
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L
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P
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F
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K
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I
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S
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160 |
ECL1 | |||||||||
Y
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Y
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F
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S
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G
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S
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D
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W
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Q
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F
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170 |
TM3 | |||||||||
G
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S
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E
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L
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C
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R
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F
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V
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T
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A
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180 |
A
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F
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Y
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C
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N
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M
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Y
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A
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S
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I
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190 |
L
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L
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M
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T
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V
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I
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S
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I
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D
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R
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200 |
ICL2 | |||||||||
F
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L
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A
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V
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V
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Y
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P
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M
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Q
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S
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210 |
TM4 | |||||||||
L
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S
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W
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R
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T
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L
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G
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R
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A
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S
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220 |
F
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T
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C
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L
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A
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I
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W
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A
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L
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A
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230 |
I
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A
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G
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V
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V
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P
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L
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L
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L
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K
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240 |
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|||||||||
ECL2 | |||||||||
E
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Q
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T
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I
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Q
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V
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P
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G
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L
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N
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250 |
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I
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T
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T
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C
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H
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D
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V
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L
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N
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E
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260 |
TM5 | |||||||||
T
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L
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L
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E
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G
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Y
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Y
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A
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Y
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Y
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270 |
F
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S
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A
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F
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S
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A
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V
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F
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F
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F
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280 |
V
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P
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L
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I
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I
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S
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T
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V
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C
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Y
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290 |
V
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S
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I
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I
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R
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C
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L
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S
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S
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S
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300 |
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ICL3 | TM6 | ||||||||
A
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V
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A
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N
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R
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S
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K
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K
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S
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R
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310 |
A
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L
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F
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L
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S
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A
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A
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V
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F
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C
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320 |
I
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F
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I
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I
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C
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F
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G
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P
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T
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N
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330 |
V
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L
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L
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I
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A
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H
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Y
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S
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F
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L
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340 |
ECL3 | TM7 | ||||||||
S
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H
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T
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S
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T
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T
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E
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A
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A
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Y
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350 |
F
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A
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Y
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L
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L
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C
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V
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C
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V
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S
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360 |
S
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I
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S
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C
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C
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I
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D
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P
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L
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I
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370 |
H8 | |||||||||
Y
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Y
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Y
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A
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S
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S
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E
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C
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Q
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R
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380 |
Y
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V
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Y
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S
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I
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L
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C
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C
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K
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E
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390 |
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C-term | |||||||||
S
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S
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D
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P
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S
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S
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Y
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N
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S
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S
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400 |
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G
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Q
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L
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M
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A
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S
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K
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M
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D
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T
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410 |
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C
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S
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S
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N
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L
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N
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N
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S
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I
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Y
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420 |
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K
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K
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L
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L
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T
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hemagglutin | ||||||||||
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FLAG | |||||||||
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TEV proteas | ||||||||||
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N-term | |||||||||
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A
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F
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I
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S
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E
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D
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A
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S
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G
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94 |
TM1 | |||||||||
Y
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L
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T
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S
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S
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W
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L
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T
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L
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F
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104 |
V
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P
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S
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V
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Y
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T
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G
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V
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F
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V
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114 |
V
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S
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L
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P
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L
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N
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I
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M
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A
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I
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124 |
ICL1 | |||||||||
V
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V
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F
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I
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L
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K
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M
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K
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V
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K
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134 |
TM2 | |||||||||
K
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P
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A
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V
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V
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Y
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M
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L
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H
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L
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144 |
A
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T
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A
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D
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V
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L
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F
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V
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S
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V
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154 |
L
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P
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F
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K
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I
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S
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Y
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Y
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F
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S
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164 |
ECL1 | TM3 | ||||||||
G
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S
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D
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W
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Q
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F
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G
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S
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E
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L
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174 |
C
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R
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F
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V
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T
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A
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A
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F
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Y
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C
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184 |
N
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M
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Y
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A
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S
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I
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L
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L
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M
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T
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194 |
V
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I
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S
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I
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D
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R
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F
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L
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A
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V
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204 |
ICL2 | |||||||||
V
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Y
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P
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M
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Q
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S
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L
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S
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W
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R
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214 |
TM4 | |||||||||
T
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L
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G
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R
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A
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S
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F
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T
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C
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L
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224 |
A
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I
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W
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A
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L
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A
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I
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A
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G
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V
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234 |
ECL2 | |||||||||
V
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P
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L
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L
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L
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K
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E
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Q
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T
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I
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244 |
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|||||||||
Q
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V
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P
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G
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L
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G
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I
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T
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T
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C
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254 |
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|||||||||
H
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D
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V
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L
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S
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E
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T
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L
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L
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E
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264 |
TM5 | |||||||||
G
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Y
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Y
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A
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Y
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Y
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F
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S
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A
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F
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274 |
S
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A
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V
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F
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F
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F
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V
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P
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L
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I
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284 |
I
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S
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T
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V
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C
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Y
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V
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S
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I
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I
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294 |
ICL3 | ||||||||
R
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C
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L
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S
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S
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S
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|||
300 |
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TM6 | ||||||||
A
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A
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N
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R
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S
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K
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K
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S
|
309 |
R
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A
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L
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F
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L
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S
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A
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A
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V
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F
|
319 |
C
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I
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F
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I
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I
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C
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F
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G
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P
|
T
|
329 |
N
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V
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L
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L
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I
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A
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H
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Y
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S
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F
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339 |
ECL3 | TM7 | ||||||||
L
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S
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H
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T
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S
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T
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T
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E
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A
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A
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349 |
Y
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F
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A
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Y
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L
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L
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C
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V
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C
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V
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359 |
S
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S
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I
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S
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C
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C
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I
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D
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P
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L
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369 |
H8 | |||||||||
I
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Y
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Y
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Y
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A
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S
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S
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E
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C
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Q
|
379 |
R
|
Y
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V
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Y
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S
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I
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L
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C
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C
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K
|
389 |
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C-term | ||||||
E
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S
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S
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D
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P
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S
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His(10) | |||||||||
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
250 | | N | G |
259 | | N | S |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: signal
Name: hemagglutinin
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: FLAG
Sequence: None
Deletions:
Positions: N-term_1 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: prot_cleavage
Name: TEV protease
Sequence: None
Deletions:
Positions: N-term_2 85:85
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: T4 Lysozyme (T4L)
Sequence: None
Deletions:
Positions: Within Receptor_3 301:303
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: His(10)
Sequence: None
Deletions:
Positions: C-term_4 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect | pFastBac1 |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 1.0 %w/v |
additive | CHS | 0.03 %w/v |
additive | sodium cholate | 0.2 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Alkylation by Iodoacetamide | None |
Proteolysis by TEV protease | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 40-50 mg/ml
Temperature: 20°C
ph : 6.0-6.5
Remarks: The protein was mixed with monoolein and cholesterol (10:1 by mass) using the two syringe mixing method by weight of 1:1.5 (protein:lipid).
Chemical lists
Type | Item | Concentration |
---|---|---|
Additional | ||
unknown | Sodium Chloride | 0.1-0.2 M |
unknown | Sodium Phosphate pH 6.0-6.5 | 100 mM |
unknown | PEG 300 | 25-35 % |
Detergent | ||
detergent | DDM | 0.1 % w/v |
Lipid | ||
lipid | CHS | 0.02 %w/v |