acm4_human
NAME
5DSG
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
acm4_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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A
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N
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F
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T
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P
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V
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N
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G
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S
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10 |
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S
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G
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N
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Q
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S
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V
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R
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L
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V
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T
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20 |
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|||||||||
TM1 | |||||||||
S
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S
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S
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H
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N
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R
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Y
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E
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T
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V
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30 |
E
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M
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V
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F
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I
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A
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T
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V
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T
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G
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40 |
S
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L
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S
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L
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V
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T
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V
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V
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G
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N
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50 |
I
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L
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V
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M
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L
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S
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I
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K
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V
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N
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60 |
ICL1 | TM2 | ||||||||
R
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Q
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L
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Q
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T
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V
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N
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N
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Y
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F
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70 |
L
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F
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S
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L
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A
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C
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A
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D
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L
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I
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80 |
I
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G
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A
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F
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S
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M
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N
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L
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Y
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T
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90 |
ECL1 | |||||||||
V
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Y
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I
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I
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K
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G
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Y
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W
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P
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L
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100 |
TM3 | |||||||||
G
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A
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V
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V
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C
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D
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L
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W
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L
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A
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110 |
L
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D
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Y
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V
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V
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S
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N
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A
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S
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V
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120 |
M
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N
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L
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L
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I
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I
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S
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F
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D
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R
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130 |
ICL2 | |||||||||
Y
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F
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C
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V
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T
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K
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P
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L
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T
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Y
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140 |
TM4 | |||||||||
P
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A
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R
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R
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T
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T
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K
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M
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A
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G
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150 |
L
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M
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I
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A
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A
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A
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W
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V
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L
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S
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160 |
F
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V
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L
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W
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A
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P
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A
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I
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L
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F
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170 |
ECL2 | |||||||||
W
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Q
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F
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V
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V
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G
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K
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R
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T
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V
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180 |
P
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D
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N
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Q
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C
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F
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I
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Q
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F
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L
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190 |
TM5 | |||||||||
S
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N
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P
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A
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V
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T
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F
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G
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T
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A
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200 |
I
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A
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A
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F
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Y
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L
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P
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V
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V
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I
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210 |
M
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T
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V
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L
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Y
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I
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H
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I
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S
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L
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220 |
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||||||
A
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S
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R
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S
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R
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V
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H
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K
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H
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R
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230 |
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ICL3 | |||||||||
P
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E
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G
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P
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K
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E
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K
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K
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A
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K
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240 |
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T
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L
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A
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F
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L
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K
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S
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P
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L
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M
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250 |
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K
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Q
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S
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V
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K
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K
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P
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P
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P
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G
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260 |
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E
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A
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A
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R
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E
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E
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L
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R
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N
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G
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270 |
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K
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L
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E
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E
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A
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P
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P
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P
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A
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L
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280 |
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P
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P
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P
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P
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R
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P
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V
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A
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D
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K
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290 |
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|
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D
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T
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S
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N
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E
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S
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S
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S
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G
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S
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300 |
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A
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T
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Q
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N
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T
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K
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E
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R
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P
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A
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310 |
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T
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E
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L
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S
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T
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T
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E
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A
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T
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T
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320 |
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P
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A
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M
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P
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A
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P
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P
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L
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Q
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P
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330 |
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R
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A
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L
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N
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P
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A
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S
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R
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W
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S
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340 |
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K
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I
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Q
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I
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V
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T
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K
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Q
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T
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G
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350 |
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N
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E
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C
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V
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T
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A
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I
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E
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I
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V
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360 |
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P
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A
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T
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P
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A
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G
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M
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R
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P
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A
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370 |
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A
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N
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V
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A
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R
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K
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F
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A
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S
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I
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380 |
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A
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R
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N
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Q
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V
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R
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K
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K
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R
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Q
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390 |
TM6 | |||||||||
M
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A
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A
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R
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E
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R
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K
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V
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T
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R
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400 |
T
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I
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F
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A
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I
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L
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L
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A
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F
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I
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410 |
L
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T
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W
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T
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P
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Y
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N
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V
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M
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V
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420 |
ECL3 | |||||||||
L
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V
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N
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T
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F
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C
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Q
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S
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C
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I
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430 |
TM7 | |||||||||
P
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D
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T
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V
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W
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S
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I
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G
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Y
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W
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440 |
L
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C
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Y
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V
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N
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S
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T
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I
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N
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P
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450 |
H8 | |||||||||
A
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C
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Y
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A
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L
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C
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N
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A
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T
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F
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460 |
K
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K
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T
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F
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R
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H
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L
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L
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L
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C
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470 |
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||||||||
C-term | ||||||||
Q
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Y
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R
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N
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I
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G
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T
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A
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R
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FLAG | |||||||||
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|||||||||
N-term | TM1 | ||||||||
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S
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S
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H
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N
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R
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Y
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E
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T
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V
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30 |
E
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M
|
V
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F
|
I
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A
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T
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V
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T
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G
|
40 |
S
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L
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S
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L
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V
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T
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V
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V
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G
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N
|
50 |
I
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L
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V
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M
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L
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S
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I
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K
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V
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N
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60 |
ICL1 | TM2 | ||||||||
R
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Q
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L
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Q
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T
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V
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N
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N
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Y
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F
|
70 |
L
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F
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S
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L
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A
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C
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A
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D
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L
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I
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80 |
I
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G
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A
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F
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S
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M
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N
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L
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Y
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T
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90 |
ECL1 | |||||||||
V
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Y
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I
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I
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K
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G
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Y
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W
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P
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L
|
100 |
TM3 | |||||||||
G
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A
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V
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V
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C
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D
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L
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W
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L
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A
|
110 |
L
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D
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Y
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V
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V
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S
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N
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A
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S
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V
|
120 |
M
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N
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L
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L
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I
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I
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S
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F
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D
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R
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130 |
ICL2 | |||||||||
Y
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F
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C
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V
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T
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K
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P
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L
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T
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Y
|
140 |
TM4 | |||||||||
P
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A
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R
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R
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T
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T
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K
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M
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A
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G
|
150 |
L
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M
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I
|
A
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A
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A
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W
|
V
|
L
|
S
|
160 |
F
|
V
|
L
|
W
|
A
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P
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A
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I
|
L
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F
|
170 |
ECL2 | |||||||||
W
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Q
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F
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V
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V
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G
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K
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R
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T
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V
|
180 |
P
|
D
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N
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Q
|
C
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F
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I
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Q
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F
|
L
|
190 |
TM5 | |||||||||
S
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N
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P
|
A
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V
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T
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F
|
G
|
T
|
A
|
200 |
I
|
A
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A
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F
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Y
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L
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P
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V
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V
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I
|
210 |
M
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T
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V
|
L
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Y
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I
|
H
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I
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S
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L
|
220 |
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A
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S
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R
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S
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R
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V
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mT4L | |||||||||
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TM6 | |||||||
Q
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M
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A
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A
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R
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E
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R
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K
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397 |
V
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T
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R
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T
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I
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F
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A
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I
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L
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L
|
407 |
A
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F
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I
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L
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T
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W
|
T
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P
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Y
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N
|
417 |
V
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M
|
V
|
L
|
V
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N
|
T
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F
|
C
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Q
|
427 |
ECL3 | TM7 | ||||||||
S
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C
|
I
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P
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D
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T
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V
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W
|
S
|
I
|
437 |
G
|
Y
|
W
|
L
|
C
|
Y
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V
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N
|
S
|
T
|
447 |
I
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N
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P
|
A
|
C
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Y
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A
|
L
|
C
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N
|
457 |
H8 | |||||||||
A
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T
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F
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K
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K
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T
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F
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R
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H
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L
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467 |
C-term | |||||||||
L
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L
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C
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Q
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Y
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R
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N
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I
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G
|
T
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477 |
|
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A
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His(8) | |||||||||
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: tag
Name: FLAG
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: fusion
Name: mT4L
Sequence: None
Deletions:
Positions: Within Receptor_1 227:389
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: His(8)
Sequence: None
Deletions:
Positions: C-term_2 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect | Bac-to-Bac |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 1.0 %w/v |
additive | CHS | 0.03 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Alkylation by Iodoacetamide | None |
Proteolysis by HRV 3C protease | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 50 mg/ml
Temperature: 20°C
ph : 5.5-8.0
Remarks: 1:1.5 parts w/w protein:lipid ratio
Chemical lists
Type | Item | Concentration |
---|---|---|
Additional | ||
unknown | MES | 100 mM |
unknown | PEG 300 | 25-40 % v/v |
unknown | EDTA pH 8.0 | 50-100 mM |
Detergent | ||
detergent | LMNG | 0.01 % w/v |
Lipid | ||
lipid | CHS | 0.001 %w/v |