smo_human
NAME
5L7I
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
smo_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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A
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A
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A
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R
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P
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A
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R
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G
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P
|
10 |
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E
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L
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P
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L
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L
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G
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L
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L
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L
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L
|
20 |
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|
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L
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L
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L
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G
|
D
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P
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G
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R
|
G
|
A
|
30 |
|
|||||||||
A
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S
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S
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G
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N
|
A
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T
|
G
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P
|
G
|
40 |
P
|
R
|
S
|
A
|
G
|
G
|
S
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A
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R
|
R
|
50 |
S
|
A
|
A
|
V
|
T
|
G
|
P
|
P
|
P
|
P
|
60 |
L
|
S
|
H
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C
|
G
|
R
|
A
|
A
|
P
|
C
|
70 |
E
|
P
|
L
|
R
|
Y
|
N
|
V
|
C
|
L
|
G
|
80 |
S
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V
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L
|
P
|
Y
|
G
|
A
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T
|
S
|
T
|
90 |
L
|
L
|
A
|
G
|
D
|
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|
D
|
S
|
Q
|
E
|
100 |
E
|
A
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H
|
G
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K
|
L
|
V
|
L
|
W
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S
|
110 |
G
|
L
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R
|
N
|
A
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P
|
R
|
C
|
W
|
A
|
120 |
V
|
I
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Q
|
P
|
L
|
L
|
C
|
A
|
V
|
Y
|
130 |
M
|
P
|
K
|
C
|
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|
N
|
D
|
R
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V
|
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|
140 |
L
|
P
|
S
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R
|
T
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L
|
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A
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T
|
150 |
R
|
G
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P
|
C
|
A
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I
|
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|
E
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R
|
E
|
160 |
R
|
G
|
W
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P
|
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|
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|
C
|
T
|
170 |
P
|
D
|
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|
F
|
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|
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|
G
|
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|
T
|
N
|
180 |
E
|
V
|
Q
|
N
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I
|
K
|
F
|
N
|
S
|
S
|
190 |
G
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Q
|
C
|
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|
V
|
P
|
L
|
V
|
R
|
T
|
200 |
D
|
N
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P
|
K
|
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|
W
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Y
|
E
|
D
|
V
|
210 |
E
|
G
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C
|
G
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I
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Q
|
C
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Q
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N
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P
|
220 |
TM1 | |||||||||
L
|
F
|
T
|
E
|
A
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E
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H
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Q
|
D
|
M
|
230 |
H
|
S
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Y
|
I
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A
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A
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F
|
G
|
A
|
V
|
240 |
T
|
G
|
L
|
C
|
T
|
L
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F
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T
|
L
|
A
|
250 |
ICL1 | |||||||||
T
|
F
|
V
|
A
|
D
|
W
|
R
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N
|
S
|
N
|
260 |
TM2 | |||||||||
R
|
Y
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P
|
A
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V
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I
|
L
|
F
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Y
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V
|
270 |
N
|
A
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C
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F
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F
|
V
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G
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S
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I
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G
|
280 |
ECL1 | |||||||||
W
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L
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A
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Q
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F
|
M
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D
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G
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A
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R
|
290 |
R
|
E
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I
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V
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C
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R
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A
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D
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G
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T
|
300 |
M
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R
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L
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G
|
E
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P
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T
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S
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N
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E
|
310 |
TM3 | |||||||||
T
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L
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S
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C
|
V
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I
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I
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F
|
V
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I
|
320 |
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|||||||||
V
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Y
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Y
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A
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L
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M
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A
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G
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V
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V
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330 |
W
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F
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V
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V
|
L
|
T
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Y
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A
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W
|
H
|
340 |
ICL2 | |||||||||
T
|
S
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F
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K
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A
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L
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G
|
T
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T
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Y
|
350 |
TM4 | |||||||||
Q
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P
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L
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S
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G
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K
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T
|
S
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Y
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F
|
360 |
H
|
L
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L
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T
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W
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S
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L
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P
|
F
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V
|
370 |
L
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T
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V
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A
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I
|
L
|
A
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V
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A
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Q
|
380 |
ECL2 | |||||||||
V
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D
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G
|
D
|
S
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V
|
S
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G
|
I
|
C
|
390 |
TM5 | |||||||||
F
|
V
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G
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Y
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K
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N
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Y
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R
|
Y
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R
|
400 |
A
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G
|
F
|
V
|
L
|
A
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P
|
I
|
G
|
L
|
410 |
V
|
L
|
I
|
V
|
G
|
G
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Y
|
F
|
L
|
I
|
420 |
|
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||||||||
R
|
G
|
V
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M
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T
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L
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F
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S
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I
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K
|
430 |
|
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ICL3 | TM6 | ||||||||
S
|
N
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H
|
P
|
G
|
L
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L
|
S
|
E
|
K
|
440 |
|
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||||||||
A
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A
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S
|
K
|
I
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N
|
E
|
T
|
M
|
L
|
450 |
R
|
L
|
G
|
I
|
F
|
G
|
F
|
L
|
A
|
F
|
460 |
G
|
F
|
V
|
L
|
I
|
T
|
F
|
S
|
C
|
H
|
470 |
F
|
Y
|
D
|
F
|
F
|
N
|
Q
|
A
|
E
|
W
|
480 |
E
|
R
|
S
|
F
|
R
|
D
|
Y
|
V
|
L
|
C
|
490 |
ECL3 | |||||||||
Q
|
A
|
N
|
V
|
T
|
I
|
G
|
L
|
P
|
T
|
500 |
K
|
Q
|
P
|
I
|
P
|
D
|
C
|
E
|
I
|
K
|
510 |
TM7 | |||||||||
N
|
R
|
P
|
S
|
L
|
L
|
V
|
E
|
K
|
I
|
520 |
N
|
L
|
F
|
A
|
M
|
F
|
G
|
T
|
G
|
I
|
530 |
H8 | |||||||||
A
|
M
|
S
|
T
|
W
|
V
|
W
|
T
|
K
|
A
|
540 |
T
|
L
|
L
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I
|
W
|
R
|
R
|
T
|
W
|
C
|
550 |
|
|
|
|
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|||||
C-term | |||||||||
R
|
L
|
T
|
G
|
Q
|
S
|
D
|
D
|
E
|
P
|
560 |
|
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K
|
R
|
I
|
K
|
K
|
S
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K
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M
|
I
|
A
|
570 |
|
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K
|
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K
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H
|
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|
L
|
L
|
580 |
|
|
|
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|
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|
|
|
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Q
|
N
|
P
|
G
|
Q
|
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L
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F
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S
|
590 |
|
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M
|
H
|
T
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V
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S
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H
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D
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P
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V
|
600 |
|
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A
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L
|
A
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F
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|
N
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|
P
|
610 |
|
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|
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S
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A
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W
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A
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|
620 |
|
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630 |
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640 |
|
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|
E
|
650 |
|
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Q
|
A
|
N
|
L
|
W
|
L
|
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|
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|
A
|
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|
660 |
|
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|
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|
G
|
670 |
|
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|
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R
|
K
|
K
|
K
|
R
|
R
|
K
|
R
|
K
|
K
|
680 |
|
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|
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|
V
|
C
|
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|
L
|
A
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|
P
|
P
|
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|
690 |
|
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|
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L
|
H
|
P
|
P
|
A
|
P
|
A
|
P
|
S
|
T
|
700 |
|
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|
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|
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|
P
|
R
|
L
|
P
|
Q
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L
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P
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R
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Q
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710 |
|
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K
|
C
|
L
|
V
|
A
|
A
|
G
|
A
|
W
|
G
|
720 |
|
|
|
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|
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|
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A
|
G
|
D
|
S
|
C
|
R
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Q
|
G
|
A
|
W
|
730 |
|
|
|
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|
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|
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T
|
L
|
V
|
S
|
N
|
P
|
F
|
C
|
P
|
E
|
740 |
|
|
|
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|
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|
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|
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P
|
S
|
P
|
P
|
Q
|
D
|
P
|
F
|
L
|
P
|
750 |
|
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S
|
A
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P
|
A
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P
|
V
|
A
|
W
|
A
|
H
|
760 |
|
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G
|
R
|
R
|
Q
|
G
|
L
|
G
|
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I
|
H
|
770 |
|
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|
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|
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|
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S
|
R
|
T
|
N
|
L
|
M
|
D
|
T
|
E
|
L
|
780 |
|
|
|
|
|
|
|
M
|
D
|
A
|
D
|
S
|
D
|
F
|
|
|||||||||
N-term | |||||||||
|
S
|
S
|
G
|
N
|
A
|
T
|
G
|
P
|
G
|
40 |
P
|
R
|
S
|
A
|
G
|
G
|
S
|
A
|
R
|
R
|
50 |
S
|
A
|
A
|
V
|
T
|
G
|
P
|
P
|
P
|
P
|
60 |
L
|
S
|
H
|
C
|
G
|
R
|
A
|
A
|
P
|
C
|
70 |
E
|
P
|
L
|
R
|
Y
|
N
|
V
|
C
|
L
|
G
|
80 |
S
|
V
|
L
|
P
|
Y
|
G
|
A
|
T
|
S
|
T
|
90 |
L
|
L
|
A
|
G
|
D
|
S
|
D
|
S
|
Q
|
E
|
100 |
E
|
A
|
H
|
G
|
K
|
L
|
V
|
L
|
W
|
S
|
110 |
G
|
L
|
R
|
N
|
A
|
P
|
R
|
C
|
W
|
A
|
120 |
V
|
I
|
Q
|
P
|
L
|
L
|
C
|
A
|
V
|
Y
|
130 |
M
|
P
|
K
|
C
|
E
|
N
|
D
|
R
|
V
|
E
|
140 |
L
|
P
|
S
|
R
|
T
|
L
|
C
|
Q
|
A
|
T
|
150 |
R
|
G
|
P
|
C
|
A
|
I
|
V
|
E
|
R
|
E
|
160 |
R
|
G
|
W
|
P
|
D
|
F
|
L
|
R
|
C
|
T
|
170 |
P
|
D
|
R
|
F
|
P
|
E
|
G
|
C
|
T
|
N
|
180 |
E
|
V
|
Q
|
N
|
I
|
K
|
F
|
N
|
S
|
S
|
190 |
G
|
Q
|
C
|
E
|
V
|
P
|
L
|
V
|
R
|
T
|
200 |
D
|
N
|
P
|
K
|
S
|
W
|
Y
|
E
|
D
|
V
|
210 |
E
|
G
|
C
|
G
|
I
|
Q
|
C
|
Q
|
N
|
P
|
220 |
TM1 | |||||||||
L
|
F
|
T
|
E
|
A
|
E
|
H
|
Q
|
D
|
M
|
230 |
H
|
S
|
Y
|
I
|
A
|
A
|
F
|
G
|
A
|
V
|
240 |
T
|
G
|
L
|
C
|
T
|
L
|
F
|
T
|
L
|
A
|
250 |
ICL1 | |||||||||
T
|
F
|
V
|
A
|
D
|
W
|
R
|
N
|
S
|
N
|
260 |
TM2 | |||||||||
R
|
Y
|
P
|
A
|
V
|
I
|
L
|
F
|
Y
|
V
|
270 |
N
|
A
|
C
|
F
|
F
|
V
|
G
|
S
|
I
|
G
|
280 |
ECL1 | |||||||||
W
|
L
|
A
|
Q
|
F
|
M
|
D
|
G
|
A
|
R
|
290 |
R
|
E
|
I
|
V
|
C
|
R
|
A
|
D
|
G
|
T
|
300 |
M
|
R
|
L
|
G
|
E
|
P
|
T
|
S
|
N
|
E
|
310 |
TM3 | |||||||||
T
|
L
|
S
|
C
|
V
|
I
|
I
|
F
|
V
|
I
|
320 |
|
|||||||||
V
|
Y
|
Y
|
A
|
L
|
M
|
A
|
G
|
F
|
V
|
330 |
W
|
F
|
V
|
V
|
L
|
T
|
Y
|
A
|
W
|
H
|
340 |
ICL2 | |||||||||
T
|
S
|
F
|
K
|
A
|
L
|
G
|
T
|
T
|
Y
|
350 |
TM4 | |||||||||
Q
|
P
|
L
|
S
|
G
|
K
|
T
|
S
|
Y
|
F
|
360 |
H
|
L
|
L
|
T
|
W
|
S
|
L
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P
|
F
|
V
|
370 |
L
|
T
|
V
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A
|
I
|
L
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A
|
V
|
A
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Q
|
380 |
ECL2 | |||||||||
V
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D
|
G
|
D
|
S
|
V
|
S
|
G
|
I
|
C
|
390 |
TM5 | |||||||||
F
|
V
|
G
|
Y
|
K
|
N
|
Y
|
R
|
Y
|
R
|
400 |
A
|
G
|
F
|
V
|
L
|
A
|
P
|
I
|
G
|
L
|
410 |
V
|
L
|
I
|
V
|
G
|
G
|
Y
|
F
|
L
|
I
|
420 |
|
||||||||
R
|
G
|
V
|
M
|
T
|
L
|
F
|
S
|
|
|
|
|
|
|
|
|
|
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|
|
BRIL (b562R | ||||||||||
|
|
|
|
|
|
|
|
|
|
|
TM6 | |||||||
S
|
K
|
I
|
N
|
E
|
T
|
M
|
L
|
450 |
R
|
L
|
G
|
I
|
F
|
G
|
F
|
L
|
A
|
F
|
460 |
G
|
F
|
V
|
L
|
I
|
T
|
F
|
S
|
C
|
H
|
470 |
F
|
Y
|
D
|
F
|
F
|
N
|
Q
|
A
|
E
|
W
|
480 |
E
|
R
|
S
|
F
|
R
|
D
|
Y
|
V
|
L
|
C
|
490 |
ECL3 | |||||||||
Q
|
A
|
N
|
V
|
T
|
I
|
G
|
L
|
P
|
T
|
500 |
K
|
Q
|
P
|
I
|
P
|
D
|
C
|
E
|
I
|
K
|
510 |
TM7 | |||||||||
N
|
R
|
P
|
S
|
L
|
L
|
V
|
E
|
K
|
I
|
520 |
N
|
L
|
F
|
A
|
M
|
F
|
G
|
T
|
G
|
I
|
530 |
H8 | |||||||||
A
|
M
|
S
|
T
|
W
|
V
|
W
|
T
|
K
|
A
|
540 |
T
|
L
|
L
|
I
|
W
|
R
|
R
|
T
|
W
|
C
|
550 |
|
||||||||
C-term | ||||||||
R
|
L
|
T
|
G
|
Q
|
|
|||
|
|
|
|
|
|
|
|
|
|
Rho1D4 | |||||||||
|
|
|
|
|
|
|
|
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
329 | | V | F |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: tag
Name: Rho1D4
Sequence: None
Deletions:
Positions: C-term_1 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: BRIL (b562RIL)
Sequence: None
Deletions:
Positions: Within Receptor_0 429:440
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Transient_transfection | HEK293S(TetR)-GnTI- | Mammalian |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 1.3 %w/v |
additive | CHS | 0.26 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Deglycosylation by Endo F (Endoglycosidase F1) | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 30 mg/ml
Temperature: 20°C
ph : 6.0-6.0
Remarks: molten lipid : protein ration 60:40
Chemical lists
Type | Item | Concentration |
---|---|---|
Salt | ||
salt | sodium acetate, | 0.1 M |
Additional | ||
unknown | zinc chloride | 0.5 mM |
unknown | ammonium fluoride | 0.1 M |
unknown | sodium acetate pH4 | 0.09 M |
unknown | sodium malonate | 0.09 M |
unknown | PEG500 DME | 27 %v/v |
Detergent | ||
detergent | DDM | 0.03 %w/v |
Lipid | ||
lipid | CHS | 0.006 %w/v |