5ht1b_human
NAME
5V54
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
5ht1b_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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E
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E
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P
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G
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A
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Q
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C
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A
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P
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10 |
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P
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P
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P
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A
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G
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S
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E
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T
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W
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V
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20 |
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P
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Q
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A
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N
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L
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S
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S
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A
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P
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S
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30 |
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Q
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N
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C
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S
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A
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K
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D
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Y
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I
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Y
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40 |
TM1 | |||||||||
Q
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D
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S
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I
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S
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L
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P
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W
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K
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V
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50 |
L
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L
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V
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M
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L
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L
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A
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L
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I
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T
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60 |
L
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A
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T
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T
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L
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S
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N
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A
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F
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V
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70 |
I
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A
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T
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V
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Y
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R
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T
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R
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K
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L
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80 |
ICL1 | TM2 | ||||||||
H
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T
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P
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A
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N
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Y
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L
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I
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A
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S
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90 |
L
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A
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V
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T
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D
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L
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L
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V
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S
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I
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100 |
L
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V
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M
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P
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I
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S
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T
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M
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Y
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T
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110 |
ECL1 | TM3 | ||||||||
V
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T
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G
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R
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W
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T
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L
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G
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Q
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V
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120 |
V
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C
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D
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F
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W
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L
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S
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S
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D
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I
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130 |
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T
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C
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C
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T
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A
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S
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I
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L
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H
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L
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140 |
C
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V
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I
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A
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L
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D
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R
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Y
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W
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A
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150 |
ICL2 | |||||||||
I
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T
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D
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A
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V
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E
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Y
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S
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A
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K
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160 |
TM4 | |||||||||
R
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T
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P
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K
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R
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A
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A
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V
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M
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I
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170 |
A
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L
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V
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W
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V
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F
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S
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I
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S
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I
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180 |
S
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L
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P
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P
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F
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F
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W
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R
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Q
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A
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190 |
ECL2 | |||||||||
K
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A
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E
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E
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E
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V
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S
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E
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C
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V
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200 |
TM5 | |||||||||
V
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N
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T
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D
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H
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I
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L
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Y
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T
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V
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210 |
Y
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S
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T
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V
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G
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A
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F
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Y
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F
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P
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220 |
T
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L
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L
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L
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I
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A
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L
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Y
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G
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R
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230 |
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I
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Y
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V
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E
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A
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R
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S
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R
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I
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L
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240 |
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ICL3 | |||||||||
K
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Q
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T
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P
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N
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R
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T
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G
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K
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R
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250 |
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L
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T
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R
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A
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Q
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L
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I
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T
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D
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S
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260 |
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P
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G
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S
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T
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S
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S
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V
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T
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S
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I
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270 |
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N
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S
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R
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V
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P
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D
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V
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P
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S
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E
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280 |
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S
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G
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S
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P
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V
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Y
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V
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N
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Q
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V
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290 |
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K
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V
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R
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V
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S
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D
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A
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L
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L
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E
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300 |
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TM6 | |||||||||
K
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K
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K
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L
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M
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A
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A
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R
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E
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R
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310 |
K
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A
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T
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K
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T
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L
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G
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I
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I
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L
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320 |
G
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A
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F
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I
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V
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C
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W
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L
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P
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F
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330 |
F
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I
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I
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S
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L
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V
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M
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P
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I
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C
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340 |
ECL3 | TM7 | ||||||||
K
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D
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A
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C
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W
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F
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H
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L
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A
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I
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350 |
F
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D
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F
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F
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T
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W
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L
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G
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Y
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L
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360 |
N
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S
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L
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I
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N
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P
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I
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I
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Y
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T
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370 |
H8 | |||||||||
M
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S
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N
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E
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D
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F
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K
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Q
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A
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F
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380 |
C-term | |||||||||
H
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K
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L
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I
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R
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F
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K
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C
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T
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S
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390 |
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hemagglutin | ||||||||||
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FLAG | |||||||||
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His(6) | |||||||||
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TEV proteas | ||||||||||
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N-term | |||||||||
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D
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Y
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I
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Y
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Q
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D
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S
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I
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S
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45 |
TM1 | |||||||||
L
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P
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W
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K
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V
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L
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L
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V
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M
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L
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55 |
L
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A
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L
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I
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T
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L
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A
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T
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T
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L
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65 |
S
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N
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A
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F
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V
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I
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A
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T
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V
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Y
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75 |
ICL1 | TM2 | ||||||||
R
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T
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R
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K
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L
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H
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T
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P
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A
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N
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85 |
Y
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L
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I
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A
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S
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L
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A
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V
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T
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D
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95 |
L
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L
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V
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S
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I
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L
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V
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M
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P
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I
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105 |
S
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T
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M
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Y
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T
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V
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T
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G
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R
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W
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115 |
ECL1 | TM3 | ||||||||
T
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L
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G
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Q
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V
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V
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C
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D
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F
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W
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125 |
L
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S
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S
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D
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I
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T
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C
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C
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T
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A
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135 |
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|||||||||
S
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I
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W
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H
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L
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C
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V
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I
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A
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L
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145 |
D
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R
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Y
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W
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A
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I
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T
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D
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A
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V
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155 |
ICL2 | TM4 | ||||||||
E
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Y
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S
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A
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K
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R
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T
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P
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K
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R
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165 |
A
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A
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V
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M
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I
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A
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L
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V
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W
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V
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175 |
F
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S
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I
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S
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I
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S
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L
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P
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P
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F
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185 |
ECL2 | |||||||||
F
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W
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R
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Q
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A
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K
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A
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E
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E
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E
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195 |
V
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S
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E
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C
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V
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V
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N
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T
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D
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H
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205 |
TM5 | |||||||||
I
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L
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Y
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T
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V
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Y
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S
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T
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V
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G
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215 |
A
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F
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Y
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F
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P
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T
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L
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L
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L
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I
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225 |
A
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L
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Y
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G
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R
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I
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Y
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V
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E
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A
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235 |
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R
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S
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R
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I
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OB1 | |||||||||
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TM6 | |||||||
L
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M
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A
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A
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R
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E
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R
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K
|
311 |
A
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T
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K
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T
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L
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G
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I
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I
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L
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G
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321 |
A
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F
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I
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V
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C
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W
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L
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P
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F
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F
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331 |
I
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I
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S
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L
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V
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M
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P
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I
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C
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K
|
341 |
ECL3 | TM7 | ||||||||
D
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A
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C
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W
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F
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H
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L
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A
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I
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F
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351 |
D
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F
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F
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T
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W
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L
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G
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Y
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L
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N
|
361 |
S
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L
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I
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N
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P
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I
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I
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Y
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T
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M
|
371 |
H8 | |||||||||
S
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N
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E
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D
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F
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K
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Q
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A
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F
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H
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381 |
C-term | ||||||||
K
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L
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I
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R
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F
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K
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C
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T
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S
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390 |
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
138 | | L | W |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: signal
Name: hemagglutinin
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: tag
Name: FLAG
Sequence: None
Deletions:
Positions: N-term_1 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: tag
Name: His(6)
Sequence: None
Deletions:
Positions: N-term_2 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: prot_cleavage
Name: TEV protease
Sequence: None
Deletions:
Positions: N-term_3 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: fusion
Name: OB1
Sequence: None
Deletions:
Positions: Within Receptor_4 240:303
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect | Bac - to - Bac Baculovirus Expression System (Invitrogen) |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 0.5 %w/v |
additive | CHS | 0.1 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Proteolysis by TEV protease | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 30-50 mg/ml
Temperature: 20°C
ph : 7.0-7.0
Remarks:
Chemical lists
Type | Item | Concentration |
---|---|---|
Detergent | ||
DDM | 0.05 %(w/v) | |
Lipid | ||
CHS | 0.01 %(w/v) | |
Additional | ||
Bis-Tris (pH7.0) | 100.0 mM | |
ammonium phosphate monobasic, | 155.0 mM | |
PEG 300 | 26.0 % v/v | |
GSH (L- Glutathione reduced) | 0.5 mM | |
SSG (L-Glutathione oxidized) | 0.5 mM |