ox2r_human
NAME
5WQC
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
ox2r_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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S
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G
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T
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K
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L
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E
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D
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S
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P
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10 |
P
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C
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R
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N
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W
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S
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S
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A
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S
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E
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20 |
L
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N
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E
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T
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Q
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E
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P
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F
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L
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N
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30 |
P
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T
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D
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Y
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D
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D
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E
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E
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F
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L
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40 |
R
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Y
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L
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W
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R
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E
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Y
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L
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H
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P
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50 |
TM1 | |||||||||
K
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E
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Y
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E
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W
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V
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L
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I
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A
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G
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60 |
Y
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I
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I
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V
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F
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V
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V
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A
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L
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I
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70 |
G
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N
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V
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L
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V
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C
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V
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A
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V
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W
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80 |
ICL1 | TM2 | ||||||||
K
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N
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H
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H
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M
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R
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T
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V
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T
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N
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90 |
Y
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F
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I
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V
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N
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L
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S
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L
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A
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D
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100 |
V
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L
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V
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T
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I
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T
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C
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L
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P
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A
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110 |
T
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L
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V
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V
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D
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I
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T
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E
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T
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W
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120 |
ECL1 | TM3 | ||||||||
F
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F
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G
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Q
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S
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L
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C
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K
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V
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I
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130 |
P
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Y
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L
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Q
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T
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V
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S
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V
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S
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V
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140 |
S
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V
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L
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T
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L
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S
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C
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I
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A
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L
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150 |
D
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R
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W
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Y
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A
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I
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C
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H
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P
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L
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160 |
ICL2 | TM4 | ||||||||
M
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F
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K
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S
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T
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A
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K
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R
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A
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R
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170 |
N
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S
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I
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V
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I
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I
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W
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I
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V
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S
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180 |
C
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I
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I
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M
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I
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P
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Q
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A
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I
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V
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190 |
ECL2 | |||||||||
M
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E
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C
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S
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T
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V
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F
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P
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G
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L
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200 |
A
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N
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K
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T
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T
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L
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F
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T
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V
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C
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210 |
TM5 | |||||||||
D
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E
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R
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W
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G
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G
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E
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I
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Y
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P
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220 |
K
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M
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Y
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H
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I
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C
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F
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F
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L
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V
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230 |
T
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Y
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M
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A
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P
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L
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C
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L
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M
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V
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240 |
L
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A
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Y
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L
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Q
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I
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F
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R
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K
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L
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250 |
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ICL3 | |||||||||
W
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C
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R
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Q
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I
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P
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G
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T
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S
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S
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260 |
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V
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V
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Q
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R
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K
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W
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K
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P
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L
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Q
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270 |
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P
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V
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S
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Q
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P
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R
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G
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P
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G
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Q
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280 |
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P
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T
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K
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S
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R
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M
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S
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A
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V
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A
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290 |
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TM6 | |||||||||
A
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E
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I
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K
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Q
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I
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R
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A
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R
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R
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300 |
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|||||||||
K
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T
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A
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R
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M
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L
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M
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I
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V
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L
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310 |
L
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V
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F
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A
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I
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C
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Y
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L
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P
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I
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320 |
S
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I
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L
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N
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V
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L
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K
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R
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V
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F
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330 |
ECL3 | TM7 | ||||||||
G
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M
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F
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A
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H
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T
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E
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D
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R
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E
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340 |
T
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V
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Y
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A
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W
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F
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T
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F
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S
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H
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350 |
W
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L
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V
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Y
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A
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N
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S
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A
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A
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N
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360 |
H8 | |||||||||
P
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I
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I
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Y
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N
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F
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L
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S
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G
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K
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370 |
F
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R
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E
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E
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F
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K
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A
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A
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F
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S
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380 |
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C-term | |||||||||
C
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C
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C
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L
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G
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V
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H
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H
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R
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Q
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390 |
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|
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E
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D
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R
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L
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T
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R
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G
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R
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T
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S
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400 |
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T
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E
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S
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R
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K
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S
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L
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T
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T
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Q
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410 |
|
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|
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I
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S
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N
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F
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D
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N
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I
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S
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K
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L
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420 |
|
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S
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E
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Q
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V
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V
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L
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T
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S
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I
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S
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430 |
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|
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T
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L
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P
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A
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A
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N
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G
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A
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G
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P
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440 |
|
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L
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Q
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N
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W
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hemagglutin | ||||||||||
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FLAG | |||||||||
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N-term | |||||||||
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G
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T
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K
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L
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E
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D
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S
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P
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P
|
11 |
C
|
R
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N
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W
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S
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S
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A
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S
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E
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L
|
21 |
N
|
E
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T
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Q
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E
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P
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F
|
L
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N
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P
|
31 |
T
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D
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Y
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D
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D
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E
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E
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F
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L
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R
|
41 |
Y
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L
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W
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R
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E
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Y
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L
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H
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P
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K
|
51 |
TM1 | |||||||||
E
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Y
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E
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W
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V
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L
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I
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A
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G
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Y
|
61 |
I
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I
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V
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F
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V
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V
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A
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L
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I
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G
|
71 |
N
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V
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L
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V
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C
|
V
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A
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V
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W
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K
|
81 |
ICL1 | TM2 | ||||||||
N
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H
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H
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M
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R
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T
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V
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T
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N
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Y
|
91 |
F
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I
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V
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N
|
L
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S
|
L
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A
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D
|
V
|
101 |
L
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V
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T
|
I
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T
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C
|
L
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P
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A
|
T
|
111 |
L
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V
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V
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D
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I
|
T
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E
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T
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W
|
F
|
121 |
ECL1 | TM3 | ||||||||
F
|
G
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Q
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S
|
L
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C
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K
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V
|
I
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P
|
131 |
Y
|
L
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Q
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T
|
V
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S
|
V
|
S
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V
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S
|
141 |
V
|
L
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T
|
L
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S
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C
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I
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A
|
L
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D
|
151 |
R
|
W
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Y
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A
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I
|
C
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H
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P
|
L
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M
|
161 |
ICL2 | TM4 | ||||||||
F
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K
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S
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T
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A
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K
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R
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A
|
R
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N
|
171 |
S
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I
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V
|
I
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I
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W
|
I
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V
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S
|
C
|
181 |
I
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I
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M
|
I
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P
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Q
|
A
|
I
|
V
|
M
|
191 |
ECL2 | |||||||||
E
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C
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S
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T
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V
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F
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P
|
G
|
L
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A
|
201 |
N
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K
|
T
|
T
|
L
|
F
|
T
|
V
|
C
|
D
|
211 |
TM5 | |||||||||
E
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R
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W
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G
|
G
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E
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I
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Y
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P
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K
|
221 |
M
|
Y
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H
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I
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C
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F
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F
|
L
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V
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T
|
231 |
Y
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M
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A
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P
|
L
|
C
|
L
|
M
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V
|
L
|
241 |
A
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Y
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L
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Q
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I
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F
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R
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K
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L
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W
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251 |
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|||
C
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R
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Q
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PGS (Pyroco | ||||||||||
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TM6 | |||||||
K
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Q
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I
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R
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A
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R
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R
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K
|
301 |
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|||||||||
T
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A
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R
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M
|
L
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M
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V
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V
|
L
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L
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311 |
V
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F
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A
|
I
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C
|
Y
|
L
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P
|
I
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S
|
321 |
I
|
L
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N
|
V
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L
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K
|
R
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V
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F
|
G
|
331 |
ECL3 | TM7 | ||||||||
M
|
F
|
A
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H
|
T
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E
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D
|
R
|
E
|
T
|
341 |
V
|
Y
|
A
|
W
|
F
|
T
|
F
|
S
|
H
|
W
|
351 |
L
|
V
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Y
|
A
|
N
|
S
|
A
|
A
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N
|
P
|
361 |
H8 | |||||||||
I
|
I
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Y
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N
|
F
|
L
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S
|
G
|
K
|
F
|
371 |
R
|
E
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E
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F
|
K
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A
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A
|
F
|
S
|
C
|
381 |
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||||||||
C-term | ||||||||
C
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C
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L
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G
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V
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His(10) | |||||||||
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
308 | | I | V |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: signal
Name: hemagglutinin
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: tag
Name: FLAG
Sequence: None
Deletions:
Positions: N-term_1 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: PGS (Pyrococcus abyssi glycogen synthase)
Sequence: None
Deletions:
Positions: Within Receptor_2 255:293
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: His(10)
Sequence: None
Deletions:
Positions: C-term_3 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect | BestBac |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 1.0 %w/v |
additive | sodium cholate | 0.2 % |
additive | CHS | 0.3 % |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Alkylation by Iodoacetamide | None |
Deglycosylation by PNGase F | None |
Detergent DDM exchanged with LMNG | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 40-50 mg/ml
Temperature: 20°C
ph : 5.8-6.2
Remarks:
Chemical lists
Type | Item | Concentration |
---|---|---|
Detergent | ||
Detergent | LMNG | 0.01 %w/v |
Lipid | ||
Lipid | CHS | 0.001 %w/v |
Additional | ||
additive | NaCl | 0.1 M |
additive | HEPES-NaOH (pH 7.5) | 20.0 mM |