ednrb_human
NAME
5XPR
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
ednrb_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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Q
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P
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P
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P
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S
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L
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C
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G
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R
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10 |
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A
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L
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V
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A
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L
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V
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L
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A
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C
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G
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20 |
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L
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S
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R
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I
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W
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G
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E
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E
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R
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G
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30 |
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|
|
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F
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P
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P
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D
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R
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A
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T
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P
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L
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L
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40 |
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Q
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T
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A
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E
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I
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M
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T
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P
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P
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T
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50 |
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K
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T
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L
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W
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P
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K
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G
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S
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N
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A
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60 |
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||||
S
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L
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A
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R
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S
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L
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A
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P
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A
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E
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70 |
V
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P
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K
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G
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D
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R
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T
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A
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G
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S
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80 |
P
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P
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R
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T
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I
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S
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P
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P
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P
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C
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90 |
TM1 | |||||||||
Q
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G
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P
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I
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E
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I
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K
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E
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T
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F
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100 |
K
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Y
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I
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N
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T
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V
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V
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S
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C
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L
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110 |
V
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F
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V
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L
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G
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I
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I
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G
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N
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S
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120 |
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|||||||||
T
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L
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L
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R
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I
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I
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Y
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K
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N
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K
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130 |
ICL1 | TM2 | ||||||||
C
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M
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R
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N
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G
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P
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N
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I
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L
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I
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140 |
A
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S
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L
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A
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L
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G
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D
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L
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L
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H
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150 |
I
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V
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I
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D
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I
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P
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I
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N
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V
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Y
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160 |
ECL1 | |||||||||
K
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L
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L
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A
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E
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D
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W
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P
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F
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G
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170 |
TM3 | |||||||||
A
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E
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M
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C
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K
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L
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V
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P
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F
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I
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180 |
Q
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K
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A
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S
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V
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G
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I
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T
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V
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L
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190 |
S
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L
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C
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A
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L
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S
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I
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D
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R
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Y
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200 |
ICL2 | |||||||||
R
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A
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V
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A
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S
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W
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S
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R
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I
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K
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210 |
TM4 | |||||||||
G
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I
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G
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V
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P
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K
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W
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T
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A
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V
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220 |
E
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I
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V
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L
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I
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W
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V
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V
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S
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V
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230 |
V
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L
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A
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V
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P
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E
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A
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I
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G
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F
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240 |
ECL2 | |||||||||
D
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I
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I
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T
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M
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D
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Y
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K
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G
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S
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250 |
Y
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L
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R
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I
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C
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L
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L
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H
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P
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V
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260 |
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TM5 | |||||||||
Q
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K
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T
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A
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F
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M
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Q
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F
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Y
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K
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270 |
T
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A
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K
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D
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W
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W
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L
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F
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S
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F
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280 |
Y
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F
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C
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L
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P
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L
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A
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I
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T
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A
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290 |
F
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F
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Y
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T
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L
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M
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T
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C
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E
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M
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300 |
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|||
L
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R
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K
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K
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S
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G
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M
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Q
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I
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A
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310 |
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|||||||||
ICL3 | TM6 | ||||||||
L
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N
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D
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H
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L
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K
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Q
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R
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R
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E
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320 |
V
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A
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K
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T
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V
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F
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C
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L
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V
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L
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330 |
V
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F
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A
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L
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C
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W
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L
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P
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L
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H
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340 |
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|||||||||
L
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S
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R
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I
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L
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K
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L
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T
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L
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Y
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350 |
ECL3 | TM7 | ||||||||
N
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Q
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N
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D
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P
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N
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R
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C
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E
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L
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360 |
L
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S
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F
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L
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L
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V
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L
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D
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Y
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I
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370 |
G
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I
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N
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M
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A
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S
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L
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N
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S
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C
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380 |
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|||||||||
I
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N
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P
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I
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A
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L
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Y
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L
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V
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S
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390 |
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H8 | |||||||||
K
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R
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F
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K
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N
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C
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F
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K
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S
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C
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400 |
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C-term | |||||||||
L
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C
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C
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W
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C
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Q
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S
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F
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E
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E
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410 |
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|
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K
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Q
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S
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L
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E
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E
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K
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Q
|
S
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C
|
420 |
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|
|
L
|
K
|
F
|
K
|
A
|
N
|
D
|
H
|
G
|
Y
|
430 |
|
|
|
|
|
|
|
|
|
|
D
|
N
|
F
|
R
|
S
|
S
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N
|
K
|
Y
|
S
|
440 |
|
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S
|
S
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
124 | | R | Y |
270 | | K | A |
342 | | S | A |
381 | | I | A |
396 | | C | A |
400 | | C | A |
405 | | C | A |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: signal
Name: hemagglutinin
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: tag
Name: FLAG
Sequence: None
Deletions:
Positions: N-term_1 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: linker
Name: AMGQPVGAP
Sequence: None
Deletions:
Positions: N-term_2 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: prot_cleavage
Name: TEV protease
Sequence: None
Deletions:
Positions: Within Receptor_3 57:66
Presence in Crystal:NO
Remarks: None
Protein_type: fusion
Name: mT4L
Sequence: None
Deletions:
Positions: Within Receptor_4 304:311
Presence in Crystal:YES
Remarks: None
Protein_type: prot_cleavage
Name: TEV protease
Sequence: None
Deletions:
Positions: C-term_5 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: EGFP-His10
Sequence: None
Deletions:
Positions: C-term_6 None:None
Presence in Crystal:NO
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect | Bac-to-Bac |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 0.5 %w/v |
additive | CHS | 0.1 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Alkylation by Iodoacetamide | None |
Detergent DDM exchanged with 0.1% LMNG | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 40 mg/ml
Temperature: 20°C
ph : 7.0-7.0
Remarks: ratio of 8:9:1 (w/w) protein:monoolein:cholesterol
Chemical lists
Type | Item | Concentration |
---|---|---|
Detergent | ||
detergent | LMNG | 0.01 %w/v |
Lipid | ||
lipid | CHS | 0.001 %w/v |
Additional | ||
PEG500DME | 20-25 % v/v | |
Na2SO4 | 100.0 mM | |
Buffer | ||
MOPS-NaOH, pH 7.0 | 100.0 mM |