cnr1_human
NAME
5XRA
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
cnr1_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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K
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S
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I
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L
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D
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G
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L
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A
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D
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10 |
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T
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T
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F
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R
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T
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I
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T
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T
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D
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L
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20 |
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L
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Y
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V
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G
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S
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N
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D
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I
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Q
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Y
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30 |
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E
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D
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I
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K
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G
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D
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M
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A
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S
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K
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40 |
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L
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G
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Y
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F
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P
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Q
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K
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F
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P
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L
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50 |
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T
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S
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F
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R
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G
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S
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P
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F
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Q
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E
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60 |
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K
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M
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T
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A
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G
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D
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N
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P
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Q
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L
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70 |
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V
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P
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A
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D
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Q
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V
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N
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I
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T
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E
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80 |
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F
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Y
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N
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K
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S
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L
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S
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S
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F
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K
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90 |
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E
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N
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E
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E
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N
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I
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Q
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C
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G
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E
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100 |
N
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F
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M
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D
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I
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E
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C
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F
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M
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V
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110 |
TM1 | |||||||||
L
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N
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P
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S
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Q
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Q
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L
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A
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I
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A
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120 |
V
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L
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S
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L
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T
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L
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G
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T
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F
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T
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130 |
V
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L
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E
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N
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L
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L
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V
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L
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C
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V
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140 |
ICL1 | |||||||||
I
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L
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H
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S
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R
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S
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L
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R
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C
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R
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150 |
TM2 | |||||||||
P
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S
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Y
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H
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F
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I
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G
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S
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L
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A
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160 |
V
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A
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D
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L
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L
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G
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S
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V
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I
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F
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170 |
V
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Y
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S
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F
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I
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D
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F
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H
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V
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F
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180 |
ECL1 | TM3 | ||||||||
H
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R
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K
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D
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S
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R
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N
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V
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F
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L
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190 |
F
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K
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L
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G
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G
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V
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T
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A
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S
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F
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200 |
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|||||||||
T
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A
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S
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V
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G
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S
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L
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F
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L
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T
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210 |
A
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I
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D
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R
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Y
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I
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S
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I
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H
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R
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220 |
ICL2 | |||||||||
P
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L
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A
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Y
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K
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R
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I
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V
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T
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R
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230 |
TM4 | |||||||||
P
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K
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A
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V
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V
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A
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F
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C
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L
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M
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240 |
W
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T
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I
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A
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I
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V
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I
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A
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V
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L
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250 |
ECL2 | |||||||||
P
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L
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L
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G
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W
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N
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C
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E
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K
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L
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260 |
Q
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S
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V
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C
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S
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D
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I
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F
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P
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H
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270 |
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TM5 | |||||||||
I
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D
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E
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T
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Y
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L
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M
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F
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W
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I
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280 |
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|||||||||
G
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V
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T
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S
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V
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L
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L
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L
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F
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I
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290 |
V
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Y
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A
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Y
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M
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Y
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I
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L
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W
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K
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300 |
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A
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H
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S
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H
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A
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V
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R
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M
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I
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Q
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310 |
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ICL3 | |||||||||
R
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G
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T
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Q
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K
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S
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I
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I
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I
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H
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320 |
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T
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S
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E
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D
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G
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K
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V
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Q
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V
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T
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330 |
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TM6 | |||||||||
R
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P
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D
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Q
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A
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R
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M
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D
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I
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R
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340 |
L
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A
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K
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T
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L
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V
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L
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I
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L
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V
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350 |
V
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L
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I
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I
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C
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W
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G
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P
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L
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L
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360 |
A
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I
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M
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V
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Y
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D
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V
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F
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G
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K
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370 |
ECL3 | TM7 | ||||||||
M
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N
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K
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L
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I
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K
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T
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V
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F
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A
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380 |
F
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C
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S
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M
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L
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C
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L
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L
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N
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S
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390 |
T
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V
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N
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P
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I
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I
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Y
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A
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L
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R
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400 |
H8 | |||||||||
S
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K
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D
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L
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R
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H
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A
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F
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R
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S
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410 |
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C-term | |||||||||
M
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F
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P
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S
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C
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E
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G
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T
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A
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Q
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420 |
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P
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L
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D
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N
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S
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M
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G
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D
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S
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D
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430 |
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C
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L
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H
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K
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H
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A
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N
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N
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A
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A
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440 |
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S
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V
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H
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R
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A
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A
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E
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S
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C
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I
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450 |
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K
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S
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T
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V
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K
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I
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A
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K
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V
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T
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460 |
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M
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S
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V
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S
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T
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D
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T
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S
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A
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E
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470 |
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A
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L
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hemagglutin | ||||||||||
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FLAG | |||||||||
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His(10) | |||||||||
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TEV proteas | ||||||||||
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N-term | |||||||||
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G
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E
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N
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F
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M
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D
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I
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E
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C
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107 |
TM1 | |||||||||
F
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M
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V
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L
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N
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P
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S
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Q
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Q
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L
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117 |
A
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I
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A
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V
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L
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S
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L
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T
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L
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G
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127 |
T
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F
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T
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V
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L
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E
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N
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L
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L
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V
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137 |
L
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C
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V
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I
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L
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H
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S
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R
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S
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L
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147 |
ICL1 | TM2 | ||||||||
R
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C
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R
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P
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S
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Y
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H
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F
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I
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G
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157 |
S
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L
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A
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V
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A
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D
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L
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L
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G
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S
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167 |
V
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I
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F
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V
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Y
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S
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F
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I
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D
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F
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177 |
ECL1 | TM3 | ||||||||
H
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V
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F
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H
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R
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K
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D
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S
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R
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N
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187 |
V
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F
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L
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F
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K
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L
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G
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G
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V
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T
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197 |
A
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S
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F
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T
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A
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S
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V
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G
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S
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L
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207 |
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|||||||||
F
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L
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A
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A
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I
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D
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R
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Y
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I
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S
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217 |
ICL2 | |||||||||
I
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H
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R
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P
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L
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A
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Y
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K
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R
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I
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227 |
TM4 | |||||||||
V
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T
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R
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P
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K
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A
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V
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V
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A
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F
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237 |
C
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L
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M
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W
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T
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I
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A
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I
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V
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I
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247 |
A
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V
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L
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P
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L
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L
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G
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W
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N
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C
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257 |
ECL2 | |||||||||
E
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K
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L
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Q
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S
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V
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C
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S
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D
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I
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267 |
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TM5 | |||||||||
F
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P
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H
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I
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D
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K
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T
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Y
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L
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M
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277 |
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|||||||||
F
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W
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I
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G
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V
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V
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S
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V
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L
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L
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287 |
L
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F
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I
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V
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Y
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A
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Y
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M
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Y
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I
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297 |
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|||||||||
L
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W
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K
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A
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H
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S
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H
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A
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V
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Flavodoxin | |||||||||
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TM6 | |||||||
P
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D
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Q
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A
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R
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M
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D
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I
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339 |
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|||||||||
E
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L
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A
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K
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T
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L
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V
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L
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I
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L
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349 |
V
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V
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L
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I
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I
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C
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W
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G
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P
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L
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359 |
L
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A
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I
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M
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V
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Y
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D
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V
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F
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G
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369 |
ECL3 | TM7 | ||||||||
K
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M
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N
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K
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L
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I
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K
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T
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V
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F
|
379 |
A
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F
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C
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S
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M
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L
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C
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L
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L
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N
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389 |
S
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T
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V
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N
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P
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I
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I
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Y
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A
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L
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399 |
H8 | |||||||||
R
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S
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K
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D
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L
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R
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H
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A
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F
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R
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409 |
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|||||||||
C-term | |||||||||
S
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M
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F
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P
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S
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||||
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
210 | | T | A |
273 | | E | K |
283 | | T | V |
340 | | R | E |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: signal
Name: hemagglutinin
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: tag
Name: FLAG
Sequence: None
Deletions:
Positions: N-term_1 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: tag
Name: His(10)
Sequence: None
Deletions:
Positions: N-term_2 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: prot_cleavage
Name: TEV protease
Sequence: None
Deletions:
Positions: N-term_3 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: fusion
Name: Flavodoxin
Sequence: None
Deletions:
Positions: Within Receptor_4 307:331
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Transient_transfection | HEK293T | Mammalian | FreeStyleTM 293 Expression system |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | LMNG | 1.0 %(w/v) |
additive | CHS | 0.2 %(w/v) |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Alkylation by Iodoacetamide | None |
Proteolysis by TEV protease | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 35 mg/ml
Temperature: 20°C
ph : 6.4-6.4
Remarks:
Chemical lists
Type | Item | Concentration |
---|---|---|
Detergent | ||
detergent | LMNG | 0.01 %(w/v) |
Lipid | ||
lipid | CHS | 0.002 %(w/v) |
Buffer | ||
sodium cacodylate trihydrate pH 6.4 | 0.1 M | |
Additional | ||
C4H4KNaO6 | 300-350 mM | |
PEG400 | 30.0 %v/v |