grm5_human
NAME
6FFI
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
grm5_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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V
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L
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L
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L
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I
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L
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S
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V
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L
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10 |
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20 |
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30 |
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40 |
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60 |
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70 |
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80 |
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90 |
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100 |
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110 |
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120 |
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E
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E
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130 |
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140 |
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|
150 |
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|
160 |
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|
170 |
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A
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A
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T
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|
180 |
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K
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190 |
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|
200 |
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A
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|
Y
|
N
|
210 |
|
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W
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T
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Y
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|
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|
220 |
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Y
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A
|
230 |
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240 |
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250 |
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|
260 |
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K
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270 |
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280 |
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M
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G
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L
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L
|
M
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A
|
M
|
290 |
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R
|
R
|
L
|
G
|
L
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A
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G
|
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|
L
|
300 |
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L
|
L
|
G
|
S
|
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W
|
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|
310 |
|
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320 |
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330 |
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|
340 |
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Y
|
L
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K
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L
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N
|
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|
350 |
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N
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360 |
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|
370 |
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K
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T
|
380 |
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C
|
N
|
S
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S
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L
|
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390 |
|
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|
400 |
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N
|
A
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Y
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M
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Y
|
G
|
410 |
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L
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N
|
M
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|
420 |
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Y
|
A
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430 |
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440 |
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450 |
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|
470 |
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|
480 |
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490 |
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M
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D
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W
|
500 |
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K
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K
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S
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N
|
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510 |
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520 |
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530 |
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C
|
W
|
T
|
C
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T
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P
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C
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K
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|
N
|
540 |
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|
Y
|
V
|
F
|
D
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E
|
Y
|
T
|
C
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K
|
550 |
|
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|
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|
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A
|
C
|
Q
|
L
|
G
|
S
|
W
|
P
|
T
|
D
|
560 |
|
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|
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|
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|
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||
D
|
L
|
T
|
G
|
C
|
D
|
L
|
I
|
P
|
V
|
570 |
|
|||||||||
TM1 | |||||||||
Q
|
Y
|
L
|
R
|
W
|
G
|
D
|
P
|
E
|
P
|
580 |
I
|
A
|
A
|
V
|
V
|
F
|
A
|
C
|
L
|
G
|
590 |
L
|
L
|
A
|
T
|
L
|
F
|
V
|
T
|
V
|
V
|
600 |
ICL1 | |||||||||
F
|
I
|
I
|
Y
|
R
|
D
|
T
|
P
|
V
|
V
|
610 |
TM2 | |||||||||
K
|
S
|
S
|
S
|
R
|
E
|
L
|
C
|
Y
|
I
|
620 |
I
|
L
|
A
|
G
|
I
|
C
|
L
|
G
|
Y
|
L
|
630 |
ECL1 | |||||||||
C
|
T
|
F
|
C
|
L
|
I
|
A
|
K
|
P
|
K
|
640 |
TM3 | |||||||||
Q
|
I
|
Y
|
C
|
Y
|
L
|
Q
|
R
|
I
|
G
|
650 |
I
|
G
|
L
|
S
|
P
|
A
|
M
|
S
|
Y
|
S
|
660 |
|
|
||||||||
A
|
L
|
V
|
T
|
K
|
T
|
N
|
R
|
I
|
A
|
670 |
|
|
|
|||||||
ICL2 | |||||||||
R
|
I
|
L
|
A
|
G
|
S
|
K
|
K
|
K
|
I
|
680 |
C
|
T
|
K
|
K
|
P
|
R
|
F
|
M
|
S
|
A
|
690 |
TM4 | |||||||||
C
|
A
|
Q
|
L
|
V
|
I
|
A
|
F
|
I
|
L
|
700 |
I
|
C
|
I
|
Q
|
L
|
G
|
I
|
I
|
V
|
A
|
710 |
ECL2 | |||||||||
L
|
F
|
I
|
M
|
E
|
P
|
P
|
D
|
I
|
M
|
720 |
H
|
D
|
Y
|
P
|
S
|
I
|
R
|
E
|
V
|
Y
|
730 |
TM5 | |||||||||
L
|
I
|
C
|
N
|
T
|
T
|
N
|
L
|
G
|
V
|
740 |
|
|||||||||
V
|
T
|
P
|
L
|
G
|
Y
|
N
|
G
|
L
|
L
|
750 |
|
|||||||||
I
|
L
|
S
|
C
|
T
|
F
|
Y
|
A
|
F
|
K
|
760 |
ICL3 | |||||||||
T
|
R
|
N
|
V
|
P
|
A
|
N
|
F
|
N
|
E
|
770 |
TM6 | |||||||||
A
|
K
|
Y
|
I
|
A
|
F
|
T
|
M
|
Y
|
T
|
780 |
T
|
C
|
I
|
I
|
W
|
L
|
A
|
F
|
V
|
P
|
790 |
ECL3 | TM7 | ||||||||||
I
|
Y
|
F
|
G
|
S
|
N
|
Y
|
K
|
I
|
I
|
||
800 |
T
|
M
|
C
|
F
|
S
|
V
|
S
|
L
|
S
|
A
|
810 |
T
|
V
|
A
|
L
|
G
|
C
|
M
|
F
|
V
|
P
|
820 |
H8 | |||||||||
K
|
V
|
Y
|
I
|
I
|
L
|
A
|
K
|
P
|
E
|
830 |
|
|
|
|
||||||
R
|
N
|
V
|
R
|
S
|
A
|
F
|
T
|
T
|
S
|
840 |
|
|
|
|
|
|
|
|
|
|
C-term | |||||||||
T
|
V
|
V
|
R
|
M
|
H
|
V
|
G
|
D
|
G
|
850 |
|
|
|
|
|
|
|
|
|
|
K
|
S
|
S
|
S
|
A
|
A
|
S
|
R
|
S
|
S
|
860 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
V
|
N
|
L
|
W
|
K
|
R
|
R
|
G
|
870 |
|
|
|
|
|
|
|
|
|
|
S
|
S
|
G
|
E
|
T
|
L
|
R
|
Y
|
K
|
D
|
880 |
|
|
|
|
|
|
|
|
|
|
R
|
R
|
L
|
A
|
Q
|
H
|
K
|
S
|
E
|
I
|
890 |
|
|
|
|
|
|
|
|
|
|
E
|
C
|
F
|
T
|
P
|
K
|
G
|
S
|
M
|
G
|
900 |
|
|
|
|
|
|
|
|
|
|
N
|
G
|
G
|
R
|
A
|
T
|
M
|
S
|
S
|
S
|
910 |
|
|
|
|
|
|
|
|
|
|
N
|
G
|
K
|
S
|
V
|
T
|
W
|
A
|
Q
|
N
|
920 |
|
|
|
|
|
|
|
|
|
|
E
|
K
|
S
|
S
|
R
|
G
|
Q
|
H
|
L
|
W
|
930 |
|
|
|
|
|
|
|
|
|
|
Q
|
R
|
L
|
S
|
I
|
H
|
I
|
N
|
K
|
K
|
940 |
|
|
|
|
|
|
|
|
|
|
E
|
N
|
P
|
N
|
Q
|
T
|
A
|
V
|
I
|
K
|
950 |
|
|
|
|
|
|
|
|
|
|
P
|
F
|
P
|
K
|
S
|
T
|
E
|
S
|
R
|
G
|
960 |
|
|
|
|
|
|
|
|
|
|
L
|
G
|
A
|
G
|
A
|
G
|
A
|
G
|
G
|
S
|
970 |
|
|
|
|
|
|
|
|
|
|
A
|
G
|
G
|
V
|
G
|
A
|
T
|
G
|
G
|
A
|
980 |
|
|
|
|
|
|
|
|
|
|
G
|
C
|
A
|
G
|
A
|
G
|
P
|
G
|
G
|
P
|
990 |
|
|
|
|
|
|
|
|
|
|
E
|
S
|
P
|
D
|
A
|
G
|
P
|
K
|
A
|
L
|
1000 |
|
|
|
|
|
|
|
|
|
|
Y
|
D
|
V
|
A
|
E
|
A
|
E
|
E
|
H
|
F
|
1010 |
|
|
|
|
|
|
|
|
|
|
P
|
A
|
P
|
A
|
R
|
P
|
R
|
S
|
P
|
S
|
1020 |
|
|
|
|
|
|
|
|
|
|
P
|
I
|
S
|
T
|
L
|
S
|
H
|
R
|
A
|
G
|
1030 |
|
|
|
|
|
|
|
|
|
|
S
|
A
|
S
|
R
|
T
|
D
|
D
|
D
|
V
|
P
|
1040 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
H
|
S
|
E
|
P
|
V
|
A
|
R
|
S
|
1050 |
|
|
|
|
|
|
|
|
|
|
S
|
S
|
S
|
Q
|
G
|
S
|
L
|
M
|
E
|
Q
|
1060 |
|
|
|
|
|
|
|
|
|
|
I
|
S
|
S
|
V
|
V
|
T
|
R
|
F
|
T
|
A
|
1070 |
|
|
|
|
|
|
|
|
|
|
N
|
I
|
S
|
E
|
L
|
N
|
S
|
M
|
M
|
L
|
1080 |
|
|
|
|
|
|
|
|
|
|
S
|
T
|
A
|
A
|
P
|
S
|
P
|
G
|
V
|
G
|
1090 |
|
|
|
|
|
|
|
|
|
|
A
|
P
|
L
|
C
|
S
|
S
|
Y
|
L
|
I
|
P
|
1100 |
|
|
|
|
|
|
|
|
|
|
K
|
E
|
I
|
Q
|
L
|
P
|
T
|
T
|
M
|
T
|
1110 |
|
|
|
|
|
|
|
|
|
|
T
|
F
|
A
|
E
|
I
|
Q
|
P
|
L
|
P
|
A
|
1120 |
|
|
|
|
|
|
|
|
|
|
I
|
E
|
V
|
T
|
G
|
G
|
A
|
Q
|
P
|
A
|
1130 |
|
|
|
|
|
|
|
|
|
|
A
|
G
|
A
|
Q
|
A
|
A
|
G
|
D
|
A
|
A
|
1140 |
|
|
|
|
|
|
|
|
|
|
R
|
E
|
S
|
P
|
A
|
A
|
G
|
P
|
E
|
A
|
1150 |
|
|
|
|
|
|
|
|
|
|
A
|
A
|
A
|
K
|
P
|
D
|
L
|
E
|
E
|
L
|
1160 |
|
|
|
|
|
|
|
|
|
|
V
|
A
|
L
|
T
|
P
|
P
|
S
|
P
|
F
|
R
|
1170 |
|
|
|
|
|
|
|
|
|
|
D
|
S
|
V
|
D
|
S
|
G
|
S
|
T
|
T
|
P
|
1180 |
|
|
|
|
|
|
|
|
|
|
N
|
S
|
P
|
V
|
S
|
E
|
S
|
A
|
L
|
C
|
1190 |
|
|
|
|
|
|
|
|
|
|
I
|
P
|
S
|
S
|
P
|
K
|
Y
|
D
|
T
|
L
|
1200 |
|
|
|
|
|
|
|
|
|
|
I
|
I
|
R
|
D
|
Y
|
T
|
Q
|
S
|
S
|
S
|
1210 |
|
|
S
|
L
|
|
|
|
|
|
|
|
|
|
|
|
GP64 signal | ||||||||||
|
|
|
|
|
|
|
|
|
|
|
|
|||||||||
N-term | |||||||||
|
P
|
V
|
Q
|
Y
|
L
|
R
|
W
|
G
|
D
|
577 |
|
|||||||||
TM1 | |||||||||
P
|
A
|
P
|
I
|
A
|
A
|
V
|
V
|
F
|
A
|
587 |
C
|
L
|
G
|
L
|
L
|
A
|
T
|
L
|
F
|
V
|
597 |
T
|
V
|
V
|
F
|
I
|
I
|
Y
|
R
|
D
|
T
|
607 |
ICL1 | TM2 | ||||||||
P
|
V
|
V
|
K
|
S
|
S
|
S
|
R
|
E
|
L
|
617 |
C
|
Y
|
I
|
I
|
L
|
A
|
G
|
I
|
C
|
L
|
627 |
G
|
Y
|
L
|
C
|
T
|
F
|
C
|
L
|
I
|
A
|
637 |
ECL1 | TM3 | ||||||||
K
|
P
|
K
|
Q
|
I
|
Y
|
C
|
Y
|
L
|
Q
|
647 |
R
|
I
|
G
|
I
|
G
|
L
|
S
|
P
|
A
|
M
|
657 |
|
|||||||||
S
|
Y
|
S
|
A
|
L
|
V
|
T
|
K
|
T
|
Y
|
667 |
|
|
||||||||
ICL2 | |||||||||
R
|
A
|
A
|
R
|
I
|
L
|
A
|
M
|
S
|
K
|
677 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|||||||
K
|
|
I
|
C
|
T
|
K
|
K
|
P
|
R
|
686 |
TM4 | |||||||||
F
|
M
|
S
|
A
|
C
|
A
|
Q
|
L
|
V
|
I
|
696 |
A
|
F
|
I
|
L
|
I
|
C
|
I
|
Q
|
L
|
G
|
706 |
I
|
I
|
V
|
A
|
L
|
F
|
I
|
M
|
E
|
P
|
716 |
ECL2 | |||||||||
P
|
D
|
I
|
M
|
H
|
D
|
Y
|
P
|
S
|
I
|
726 |
R
|
E
|
V
|
Y
|
L
|
I
|
C
|
N
|
T
|
T
|
736 |
|
|||||||||
TM5 | |||||||||
N
|
L
|
G
|
V
|
V
|
A
|
P
|
L
|
G
|
Y
|
746 |
|
|||||||||
N
|
G
|
L
|
L
|
I
|
L
|
A
|
C
|
T
|
F
|
756 |
ICL3 | |||||||||
Y
|
A
|
F
|
K
|
T
|
R
|
N
|
V
|
P
|
A
|
766 |
TM6 | |||||||||
N
|
F
|
N
|
E
|
A
|
K
|
Y
|
I
|
A
|
F
|
776 |
T
|
M
|
Y
|
T
|
T
|
C
|
I
|
I
|
W
|
L
|
786 |
A
|
F
|
V
|
P
|
I
|
Y
|
F
|
G
|
S
|
N
|
796 |
TM7 | |||||||||
Y
|
K
|
I
|
I
|
T
|
M
|
C
|
F
|
S
|
V
|
806 |
S
|
L
|
S
|
A
|
T
|
V
|
A
|
L
|
G
|
C
|
816 |
M
|
F
|
V
|
P
|
K
|
V
|
Y
|
I
|
I
|
L
|
826 |
|
|||||||||||||||||||||
H8 | |||||||||||||||||||||
A
|
K
|
P
|
E
|
R
|
N
|
V
|
R
|
S
|
A
|
|
|||||||||||
836 |
|
|
|
|
|
|
|
|
|
|
His(10) | |||||||||
|
|
|
|
|
|
|
|
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
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|
|
|
|
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|
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|
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|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
579 | | E | A |
667 | | N | Y |
669 | | I | A |
675 | | G | M |
742 | | T | A |
753 | | S | A |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: signal
Name: GP64 signal
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:NO
Remarks: None
Protein_type: fusion
Name: T4 Lysozyme (T4L)
Sequence: None
Deletions:
Positions: Within Receptor_1 678:679
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: His(10)
Sequence: None
Deletions:
Positions: C-term_2 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf21 | Insect |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 1.5 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
NONE | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 40.0 mg/ml
Temperature: 20°C
ph : 5.5-6.8
Remarks: With M-PEP ligand(NAM)
Chemical lists
Type | Item | Concentration |
---|---|---|
Detergent | ||
DDM | 0.03 % w/v | |
Additional | ||
MES pH 5.5–6.8 | 100.0 mM | |
PEG 400 | 24-34 % v/v | |
ammonium phosphate dibasic | 100-250 mM |