nk1r_human
NAME
6J21
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
nk1r_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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|||||||||
N-term | |||||||||
M
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D
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N
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V
|
L
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P
|
V
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D
|
S
|
D
|
10 |
L
|
S
|
P
|
N
|
I
|
S
|
T
|
N
|
T
|
S
|
20 |
TM1 | |||||||||
E
|
P
|
N
|
Q
|
F
|
V
|
Q
|
P
|
A
|
W
|
30 |
Q
|
I
|
V
|
L
|
W
|
A
|
A
|
A
|
Y
|
T
|
40 |
V
|
I
|
V
|
V
|
T
|
S
|
V
|
V
|
G
|
N
|
50 |
V
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V
|
V
|
M
|
W
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I
|
I
|
L
|
A
|
H
|
60 |
ICL1 | TM2 | ||||||||
K
|
R
|
M
|
R
|
T
|
V
|
T
|
N
|
Y
|
F
|
70 |
L
|
V
|
N
|
L
|
A
|
F
|
A
|
E
|
A
|
S
|
80 |
M
|
A
|
A
|
F
|
N
|
T
|
V
|
V
|
N
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F
|
90 |
ECL1 | |||||||||
T
|
Y
|
A
|
V
|
H
|
N
|
E
|
W
|
Y
|
Y
|
100 |
TM3 | |||||||||
G
|
L
|
F
|
Y
|
C
|
K
|
F
|
H
|
N
|
F
|
110 |
F
|
P
|
I
|
A
|
A
|
V
|
F
|
A
|
S
|
I
|
120 |
Y
|
S
|
M
|
T
|
A
|
V
|
A
|
F
|
D
|
R
|
130 |
ICL2 | |||||||||
Y
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M
|
A
|
I
|
I
|
H
|
P
|
L
|
Q
|
P
|
140 |
TM4 | |||||||||
R
|
L
|
S
|
A
|
T
|
A
|
T
|
K
|
V
|
V
|
150 |
I
|
C
|
V
|
I
|
W
|
V
|
L
|
A
|
L
|
L
|
160 |
L
|
A
|
F
|
P
|
Q
|
G
|
Y
|
Y
|
S
|
T
|
170 |
ECL2 | |||||||||
T
|
E
|
T
|
M
|
P
|
S
|
R
|
V
|
V
|
C
|
180 |
TM5 | |||||||||
M
|
I
|
E
|
W
|
P
|
E
|
H
|
P
|
N
|
K
|
190 |
I
|
Y
|
E
|
K
|
V
|
Y
|
H
|
I
|
C
|
V
|
200 |
T
|
V
|
L
|
I
|
Y
|
F
|
L
|
P
|
L
|
L
|
210 |
V
|
I
|
G
|
Y
|
A
|
Y
|
T
|
V
|
V
|
G
|
220 |
|
|
|
|
||||||
I
|
T
|
L
|
W
|
A
|
S
|
E
|
I
|
P
|
G
|
230 |
|
|
|
|
|
|
||||
ICL3 | TM6 | ||||||||
D
|
S
|
S
|
D
|
R
|
Y
|
H
|
E
|
Q
|
V
|
240 |
S
|
A
|
K
|
R
|
K
|
V
|
V
|
K
|
M
|
M
|
250 |
I
|
V
|
V
|
V
|
C
|
T
|
F
|
A
|
I
|
C
|
260 |
W
|
L
|
P
|
F
|
H
|
I
|
F
|
F
|
L
|
L
|
270 |
ECL3 | |||||||||
P
|
Y
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I
|
N
|
P
|
D
|
L
|
Y
|
L
|
K
|
280 |
TM7 | |||||||||
K
|
F
|
I
|
Q
|
Q
|
V
|
Y
|
L
|
A
|
I
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290 |
M
|
W
|
L
|
A
|
M
|
S
|
S
|
T
|
M
|
Y
|
300 |
H8 | |||||||||
N
|
P
|
I
|
I
|
Y
|
C
|
C
|
L
|
N
|
D
|
310 |
R
|
F
|
R
|
L
|
G
|
F
|
K
|
H
|
A
|
F
|
320 |
C-term | |||||||||
R
|
C
|
C
|
P
|
F
|
I
|
S
|
A
|
G
|
D
|
330 |
|
|
|
|
|
|||||
Y
|
E
|
G
|
L
|
E
|
M
|
K
|
S
|
T
|
R
|
340 |
|
|
|
|
|
|
|
|
|
|
Y
|
L
|
Q
|
T
|
Q
|
G
|
S
|
V
|
Y
|
K
|
350 |
|
|
|
|
|
|
|
|
|
|
V
|
S
|
R
|
L
|
E
|
T
|
T
|
I
|
S
|
T
|
360 |
|
|
|
|
|
|
|
|
|
|
V
|
V
|
G
|
A
|
H
|
E
|
E
|
E
|
P
|
E
|
370 |
|
|
|
|
|
|
|
|
|
|
D
|
G
|
P
|
K
|
A
|
T
|
P
|
S
|
S
|
L
|
380 |
|
|
|
|
|
|
|
|
|
|
D
|
L
|
T
|
S
|
N
|
C
|
S
|
S
|
R
|
S
|
390 |
|
|
|
|
|
|
|
|
|
|
D
|
S
|
K
|
T
|
M
|
T
|
E
|
S
|
F
|
S
|
400 |
|
|
|
|
|
|
|
F
|
S
|
S
|
N
|
V
|
L
|
S
|
|
|||||||||
N-term | |||||||||
|
D
|
N
|
V
|
L
|
P
|
V
|
D
|
S
|
D
|
10 |
L
|
S
|
P
|
N
|
I
|
S
|
T
|
N
|
T
|
S
|
20 |
TM1 | |||||||||
E
|
P
|
N
|
Q
|
F
|
V
|
Q
|
P
|
A
|
W
|
30 |
Q
|
I
|
V
|
L
|
W
|
A
|
A
|
A
|
Y
|
T
|
40 |
V
|
I
|
V
|
V
|
T
|
S
|
V
|
V
|
G
|
N
|
50 |
V
|
V
|
V
|
M
|
W
|
I
|
I
|
L
|
A
|
H
|
60 |
ICL1 | TM2 | ||||||||
K
|
R
|
M
|
R
|
T
|
V
|
T
|
N
|
Y
|
F
|
70 |
L
|
V
|
N
|
L
|
A
|
F
|
A
|
E
|
A
|
S
|
80 |
M
|
A
|
A
|
F
|
N
|
T
|
V
|
V
|
N
|
F
|
90 |
ECL1 | |||||||||
T
|
Y
|
A
|
V
|
H
|
N
|
E
|
W
|
Y
|
Y
|
100 |
TM3 | |||||||||
G
|
L
|
F
|
Y
|
C
|
K
|
F
|
H
|
N
|
F
|
110 |
F
|
P
|
I
|
A
|
A
|
V
|
F
|
A
|
S
|
I
|
120 |
Y
|
S
|
M
|
T
|
A
|
V
|
A
|
F
|
D
|
R
|
130 |
ICL2 | |||||||||
Y
|
M
|
A
|
I
|
I
|
H
|
P
|
L
|
Q
|
P
|
140 |
TM4 | |||||||||
R
|
L
|
S
|
A
|
T
|
A
|
T
|
K
|
V
|
V
|
150 |
I
|
C
|
V
|
I
|
W
|
V
|
L
|
A
|
L
|
L
|
160 |
L
|
A
|
F
|
P
|
Q
|
G
|
Y
|
Y
|
S
|
T
|
170 |
ECL2 | |||||||||
T
|
E
|
T
|
M
|
P
|
S
|
R
|
V
|
V
|
C
|
180 |
TM5 | |||||||||
M
|
I
|
E
|
W
|
P
|
E
|
H
|
P
|
N
|
K
|
190 |
I
|
Y
|
E
|
K
|
V
|
Y
|
H
|
I
|
C
|
V
|
200 |
T
|
V
|
L
|
I
|
Y
|
F
|
L
|
P
|
L
|
L
|
210 |
V
|
I
|
G
|
Y
|
A
|
Y
|
T
|
V
|
V
|
G
|
220 |
|
||||||
I
|
T
|
L
|
W
|
A
|
S
|
|
|
|
|
|
|
|
|
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|
|
Linker | |||||||||
|
|
|
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|
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|
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|
|
T4 Lysozyme | ||||||||||
|
|
|
|
|
|
|
|
|
|
|
TM6 | ||
H
|
E
|
Q
|
239 |
V
|
S
|
A
|
K
|
R
|
K
|
V
|
V
|
K
|
M
|
249 |
M
|
I
|
V
|
V
|
V
|
C
|
T
|
F
|
A
|
I
|
259 |
C
|
W
|
L
|
P
|
F
|
H
|
I
|
F
|
F
|
L
|
269 |
ECL3 | |||||||||
L
|
P
|
Y
|
I
|
N
|
P
|
D
|
L
|
Y
|
L
|
279 |
TM7 | |||||||||
K
|
K
|
F
|
I
|
Q
|
Q
|
V
|
Y
|
L
|
A
|
289 |
I
|
M
|
W
|
L
|
A
|
M
|
S
|
S
|
T
|
M
|
299 |
Y
|
N
|
P
|
I
|
I
|
Y
|
C
|
C
|
L
|
N
|
309 |
H8 | |||||||||
D
|
R
|
F
|
R
|
L
|
G
|
F
|
K
|
H
|
A
|
319 |
C-term | |||||||||
F
|
R
|
C
|
C
|
P
|
F
|
I
|
S
|
A
|
G
|
329 |
|
||||||
D
|
Y
|
E
|
G
|
L
|
E
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: auto
Name: Linker
Sequence: None
Deletions:
Positions: Within Receptor_0 227:227
Presence in Crystal:YES
Remarks: None
Protein_type: fusion
Name: T4 Lysozyme (T4L)
Sequence: None
Deletions:
Positions: Within Receptor_1 227:227
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|