GPCRdb

cnr2_human

NAME

6KPC

ORGANISM

Human (Homo sapiens)

PROTEIN NAME

cnr2_human

SOURCE

SWISSPROT

SEQUENCE

Perspective: Wild-type Construct


N-term
M	E	E	C	W	V	T	E	I	A
10



N	G	S	K	D	G	L	D	S	N
20



P	M	K	D	Y	M	I	L	S	G
30


TM1
P	Q	K	T	A	V	A	V	L	C
40



T	L	L	G	L	L	S	A	L	E
50



N	V	A	V	L	Y	L	I	L	S
60


	ICL1					TM2
S	H	Q	L	R	R	K	P	S	Y
70



L	F	I	G	S	L	A	G	A	D
80



F	L	A	S	V	V	F	A	C	S
90


						ECL1
F	V	N	F	H	V	F	H	G	V
100


	TM3
D	S	K	A	V	F	L	L	K	I
110



G	S	V	T	M	T	F	T	A	S
120



V	G	S	L	L	L	T	A	I	D
130



R	Y	L	C	L	R	Y	P	P	S
140


ICL2					TM4
Y	K	A	L	L	T	R	G	R	A
150



L	V	T	L	G	I	M	W	V	L
160



S	A	L	V	S	Y	L	P	L	M
170


	ECL2
G	W	T	C	C	P	R	P	C	S
180


						TM5
E	L	F	P	L	I	P	N	D	Y
190



L	L	S	W	L	L	F	I	A	F
200



L	F	S	G	I	I	Y	T	Y	G
210



H	V	L	W	K	A	H	Q	H	V
220


					ICL3
A	S	L	S	G	H	Q	D	R	Q
230


							TM6
V	P	G	M	A	R	M	R	L	D
240



V	R	L	A	K	T	L	G	L	V
250



L	A	V	L	L	I	C	W	F	P
260



V	L	A	L	M	A	H	S	L	A
270


	ECL3				TM7
T	T	L	S	D	Q	V	K	K	A
280



F	A	F	C	S	M	L	C	L	I
290



N	S	M	V	N	P	V	I	Y	A
300


		H8
L	R	S	G	E	I	R	S	S	A
310



H	H	C	L	A	H	W	K	K	C
320


C-term
V	R	G	L	G	S	E	A	K	E
330



E	A	P	R	S	S	V	T	E	T
340



E	A	D	G	K	I	T	P	W	P
350



D	S	R	D	L	D	L	S	D	C
360


Initiating


	N-term
	P	M	K	D	Y	M	I	L	S
29


TM1
G	P	Q	K	T	A	V	A	V	L
39



C	T	L	L	G	L	L	S	A	L
49



E	N	V	A	V	L	Y	L	I	L
59


		ICL1					TM2
S	S	H	Q	L	R	R	K	P	S
69



Y	L	F	I	G	S	L	A	G	A
79



D	F	L	A	S	V	V	F	A	C
89



S	F	V	N	F	H	V	F	H	G
99


ECL1				TM3
V	D	S	K	A	V	F	L	L	K
109



I	G	S	V	T	M	T	F	T	A
119



S	V	G	S	L	L	L	T	A	I
129



D	R	Y	L	C	L	R	Y	P	P
139


ICL2						TM4
S	Y	K	A	L	L	T	R	G	R
149



A	L	V	T	L	G	I	M	W	V
159



L	S	A	L	V	S	Y	L	P	L
169


		ECL2
M	G	W	T	C	C	P	R	P	C
179


							TM5
S	E	L	F	P	L	I	P	N	D
189



Y	L	L	S	W	L	L	F	I	A
199



F	L	F	S	G	I	I	Y	T	Y
209



G	H	V	L	W	K	A	H	Q	H
219



V	A	S


T4 Lysozyme


ICL3				TM6
A	R	M	R	L	D	V	R
242



L	A	K	T	L	G	L	V	L	A
252



V	L	L	I	C	W	F	P	V	L
262



A	L	M	A	H	S	L	A	T	T
272


ECL3				TM7
L	S	D	Q	V	K	K	A	F	A
282



F	C	S	M	L	C	L	I	N	S
292



M	V	N	P	V	I	Y	A	L	R
302


H8
S	G	E	I	R	S	S	A	H	H
312



C	L	A	H	W	K	K	C	V	R
322


C-term
G	L	G


Expression

SCHEMATIC

Perspective: Wild-type Construct

N-term

TM1

ICL1

TM2

ECL1

TM3

ICL2

TM4

ECL2

TM5

ICL3

TM6

ECL3

TM7

H8

C-term

Initia

N-term

TM1

ICL1

TM2

ECL1

TM3

ICL2

TM4

ECL2

TM5

T4 Lys

ICL3

TM6

ECL3

TM7

H8

C-term

Expres

DIAGRAMS

MUTATIONS

Position	Wild-type	Mutated

DELETIONS

from 1 to 20

from 223 to 234

from 326 to 360

MODIFICATIONS

AUXILIARY PROTEINS

Protein_type: auto

Name: Expression tag

Sequence: None

Deletions:

Positions: C-term_2 None:None

Presence in Crystal:YES

Remarks: None

Protein_type: auto

Name: Initiating methionine

Sequence: None

Deletions:

Positions: N-term_0 None:None

Presence in Crystal:YES

Remarks: None

Protein_type: fusion

Name: T4 Lysozyme (T4L)

Sequence: None

Deletions:

Positions: Within Receptor_1 223:223

Presence in Crystal:YES

Remarks: None

EXPRESSION

Expression method	Host cell type	Host cell	Remarks

SOLUBILIZATION

Solvent type	Item	Concentration

Remarks:

PURIFICATION

Purification type	Description

Remarks:

CRYSTALLIZATION

METHOD:

TYPE: ()

PROTEIN CONCENTRATION:

Temperature:

ph : -

Remarks:

Chemical lists

Type	Item	Concentration