acm1_human
NAME
6OIJ
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
acm1_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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||||||||
N-term | |||||||||
M
|
N
|
T
|
S
|
A
|
P
|
P
|
A
|
V
|
S
|
10 |
|
|||||||||
P
|
N
|
I
|
T
|
V
|
L
|
A
|
P
|
G
|
K
|
20 |
TM1 | |||||||||
G
|
P
|
W
|
Q
|
V
|
A
|
F
|
I
|
G
|
I
|
30 |
T
|
T
|
G
|
L
|
L
|
S
|
L
|
A
|
T
|
V
|
40 |
T
|
G
|
N
|
L
|
L
|
V
|
L
|
I
|
S
|
F
|
50 |
ICL1 | TM2 | ||||||||
K
|
V
|
N
|
T
|
E
|
L
|
K
|
T
|
V
|
N
|
60 |
N
|
Y
|
F
|
L
|
L
|
S
|
L
|
A
|
C
|
A
|
70 |
D
|
L
|
I
|
I
|
G
|
T
|
F
|
S
|
M
|
N
|
80 |
L
|
Y
|
T
|
T
|
Y
|
L
|
L
|
M
|
G
|
H
|
90 |
ECL1 | TM3 | ||||||||
W
|
A
|
L
|
G
|
T
|
L
|
A
|
C
|
D
|
L
|
100 |
|
|||||||||
W
|
L
|
A
|
L
|
D
|
Y
|
V
|
A
|
S
|
N
|
110 |
A
|
S
|
V
|
M
|
N
|
L
|
L
|
L
|
I
|
S
|
120 |
F
|
D
|
R
|
Y
|
F
|
S
|
V
|
T
|
R
|
P
|
130 |
ICL2 | TM4 | ||||||||
L
|
S
|
Y
|
R
|
A
|
K
|
R
|
T
|
P
|
R
|
140 |
R
|
A
|
A
|
L
|
M
|
I
|
G
|
L
|
A
|
W
|
150 |
L
|
V
|
S
|
F
|
V
|
L
|
W
|
A
|
P
|
A
|
160 |
I
|
L
|
F
|
W
|
Q
|
Y
|
L
|
V
|
G
|
E
|
170 |
ECL2 | |||||||||
R
|
T
|
V
|
L
|
A
|
G
|
Q
|
C
|
Y
|
I
|
180 |
TM5 | |||||||||
Q
|
F
|
L
|
S
|
Q
|
P
|
I
|
I
|
T
|
F
|
190 |
G
|
T
|
A
|
M
|
A
|
A
|
F
|
Y
|
L
|
P
|
200 |
V
|
T
|
V
|
M
|
C
|
T
|
L
|
Y
|
W
|
R
|
210 |
I
|
Y
|
R
|
E
|
T
|
E
|
N
|
R
|
A
|
R
|
220 |
ICL3 | |||||||||
E
|
L
|
A
|
A
|
L
|
Q
|
G
|
S
|
E
|
T
|
230 |
|
|
|
|
|
|
|
|
||
P
|
G
|
K
|
G
|
G
|
G
|
S
|
S
|
S
|
S
|
240 |
|
|
|
|
|
|
|
|
|
|
S
|
E
|
R
|
S
|
Q
|
P
|
G
|
A
|
E
|
G
|
250 |
|
|
|
|
|
|
|
|
|
|
S
|
P
|
E
|
T
|
P
|
P
|
G
|
R
|
C
|
C
|
260 |
|
|
|
|
|
|
|
|
|
|
R
|
C
|
C
|
R
|
A
|
P
|
R
|
L
|
L
|
Q
|
270 |
|
|
|
|
|
|
|
|
|
|
A
|
Y
|
S
|
W
|
K
|
E
|
E
|
E
|
E
|
E
|
280 |
|
|
|
|
|
|
|
|
|
|
D
|
E
|
G
|
S
|
M
|
E
|
S
|
L
|
T
|
S
|
290 |
|
|
|
|
|
|
|
|
|
|
S
|
E
|
G
|
E
|
E
|
P
|
G
|
S
|
E
|
V
|
300 |
|
|
|
|
|
|
|
|
|
|
V
|
I
|
K
|
M
|
P
|
M
|
V
|
D
|
P
|
E
|
310 |
|
|
|
|
|
|
|
|
|
|
A
|
Q
|
A
|
P
|
T
|
K
|
Q
|
P
|
P
|
R
|
320 |
|
|
|
|
|
|
|
|
|
|
S
|
S
|
P
|
N
|
T
|
V
|
K
|
R
|
P
|
T
|
330 |
|
|
|
|
|
|
|
|
|
|
K
|
K
|
G
|
R
|
D
|
R
|
A
|
G
|
K
|
G
|
340 |
|
|
|
|
||||||
Q
|
K
|
P
|
R
|
G
|
K
|
E
|
Q
|
L
|
A
|
350 |
TM6 | |||||||||
K
|
R
|
K
|
T
|
F
|
S
|
L
|
V
|
K
|
E
|
360 |
K
|
K
|
A
|
A
|
R
|
T
|
L
|
S
|
A
|
I
|
370 |
L
|
L
|
A
|
F
|
I
|
L
|
T
|
W
|
T
|
P
|
380 |
Y
|
N
|
I
|
M
|
V
|
L
|
V
|
S
|
T
|
F
|
390 |
ECL3 | TM7 | ||||||||
C
|
K
|
D
|
C
|
V
|
P
|
E
|
T
|
L
|
W
|
400 |
E
|
L
|
G
|
Y
|
W
|
L
|
C
|
Y
|
V
|
N
|
410 |
S
|
T
|
I
|
N
|
P
|
M
|
C
|
Y
|
A
|
L
|
420 |
H8 | |||||||||
C
|
N
|
K
|
A
|
F
|
R
|
D
|
T
|
F
|
R
|
430 |
L
|
L
|
L
|
L
|
C
|
R
|
W
|
D
|
K
|
R
|
440 |
C-term | |||||||||
R
|
W
|
R
|
K
|
I
|
P
|
K
|
R
|
P
|
G
|
450 |
S
|
V
|
H
|
R
|
T
|
P
|
S
|
R
|
Q
|
C
|
460 |
|
|
|
|
|
|
|
|
|
|
|
Expression | ||||||||||
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|
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|
|
|
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||||||||
N-term | |||||||||
|
Q
|
T
|
S
|
A
|
P
|
P
|
A
|
V
|
S
|
10 |
|
|||||||||
P
|
Q
|
I
|
T
|
V
|
L
|
A
|
P
|
G
|
K
|
20 |
TM1 | |||||||||
G
|
P
|
W
|
Q
|
V
|
A
|
F
|
I
|
G
|
I
|
30 |
T
|
T
|
G
|
L
|
L
|
S
|
L
|
A
|
T
|
V
|
40 |
T
|
G
|
N
|
L
|
L
|
V
|
L
|
I
|
S
|
F
|
50 |
ICL1 | TM2 | ||||||||
K
|
V
|
N
|
T
|
E
|
L
|
K
|
T
|
V
|
N
|
60 |
N
|
Y
|
F
|
L
|
L
|
S
|
L
|
A
|
C
|
A
|
70 |
D
|
L
|
I
|
I
|
G
|
T
|
F
|
S
|
M
|
N
|
80 |
L
|
Y
|
T
|
T
|
Y
|
L
|
L
|
M
|
G
|
H
|
90 |
ECL1 | TM3 | ||||||||
W
|
A
|
L
|
G
|
T
|
L
|
A
|
C
|
D
|
L
|
100 |
|
|||||||||
W
|
L
|
A
|
L
|
D
|
Y
|
V
|
A
|
S
|
Q
|
110 |
A
|
S
|
V
|
M
|
N
|
L
|
L
|
L
|
I
|
S
|
120 |
F
|
D
|
R
|
Y
|
F
|
S
|
V
|
T
|
R
|
P
|
130 |
ICL2 | TM4 | ||||||||
L
|
S
|
Y
|
R
|
A
|
K
|
R
|
T
|
P
|
R
|
140 |
R
|
A
|
A
|
L
|
M
|
I
|
G
|
L
|
A
|
W
|
150 |
L
|
V
|
S
|
F
|
V
|
L
|
W
|
A
|
P
|
A
|
160 |
I
|
L
|
F
|
W
|
Q
|
Y
|
L
|
V
|
G
|
E
|
170 |
ECL2 | |||||||||
R
|
T
|
V
|
L
|
A
|
G
|
Q
|
C
|
Y
|
I
|
180 |
TM5 | |||||||||
Q
|
F
|
L
|
S
|
Q
|
P
|
I
|
I
|
T
|
F
|
190 |
G
|
T
|
A
|
M
|
A
|
A
|
F
|
Y
|
L
|
P
|
200 |
V
|
T
|
V
|
M
|
C
|
T
|
L
|
Y
|
W
|
R
|
210 |
I
|
Y
|
R
|
E
|
T
|
E
|
N
|
R
|
A
|
R
|
220 |
ICL3 | |||||||||
E
|
L
|
A
|
A
|
L
|
Q
|
G
|
S
|
E
|
T
|
230 |
|
|||||||||
P
|
G
|
|
G
|
K
|
E
|
Q
|
L
|
A
|
K
|
351 |
TM6 | |||||||||
R
|
K
|
T
|
F
|
S
|
L
|
V
|
K
|
E
|
K
|
361 |
K
|
A
|
A
|
R
|
T
|
L
|
S
|
A
|
I
|
L
|
371 |
L
|
A
|
F
|
I
|
L
|
T
|
W
|
T
|
P
|
Y
|
381 |
N
|
I
|
M
|
V
|
L
|
V
|
S
|
T
|
F
|
C
|
391 |
ECL3 | TM7 | ||||||||
K
|
D
|
C
|
V
|
P
|
E
|
T
|
L
|
W
|
E
|
401 |
L
|
G
|
Y
|
W
|
L
|
C
|
Y
|
V
|
N
|
S
|
411 |
T
|
I
|
N
|
P
|
M
|
C
|
Y
|
A
|
L
|
C
|
421 |
H8 | |||||||||
N
|
K
|
A
|
F
|
R
|
D
|
T
|
F
|
R
|
L
|
431 |
C-term | |||||||||
L
|
L
|
L
|
C
|
R
|
W
|
D
|
K
|
R
|
R
|
441 |
W
|
R
|
K
|
I
|
P
|
K
|
R
|
P
|
G
|
S
|
451 |
V
|
H
|
R
|
T
|
P
|
S
|
R
|
Q
|
C
|
460 |
|
|
|
|
|
|
|
|
|
|
|
Expression | ||||||||||
|
|
|
|
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|
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|
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|
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SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
2 | | N | Q |
12 | | N | Q |
110 | | N | Q |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: auto
Name: Expression tag
Sequence: None
Deletions:
Positions: C-term_1 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: auto
Name: Expression tag
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|