acm2_human
NAME
6OIK
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
acm2_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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|
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|||||
N-term | |||||||||
M
|
N
|
N
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S
|
T
|
N
|
S
|
S
|
N
|
N
|
10 |
S
|
L
|
A
|
L
|
T
|
S
|
P
|
Y
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K
|
T
|
20 |
TM1 | |||||||||
F
|
E
|
V
|
V
|
F
|
I
|
V
|
L
|
V
|
A
|
30 |
G
|
S
|
L
|
S
|
L
|
V
|
T
|
I
|
I
|
G
|
40 |
N
|
I
|
L
|
V
|
M
|
V
|
S
|
I
|
K
|
V
|
50 |
ICL1 | TM2 | ||||||||
N
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R
|
H
|
L
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Q
|
T
|
V
|
N
|
N
|
Y
|
60 |
F
|
L
|
F
|
S
|
L
|
A
|
C
|
A
|
D
|
L
|
70 |
I
|
I
|
G
|
V
|
F
|
S
|
M
|
N
|
L
|
Y
|
80 |
T
|
L
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Y
|
T
|
V
|
I
|
G
|
Y
|
W
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P
|
90 |
ECL1 | TM3 | ||||||||
L
|
G
|
P
|
V
|
V
|
C
|
D
|
L
|
W
|
L
|
100 |
A
|
L
|
D
|
Y
|
V
|
V
|
S
|
N
|
A
|
S
|
110 |
V
|
M
|
N
|
L
|
L
|
I
|
I
|
S
|
F
|
D
|
120 |
R
|
Y
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F
|
C
|
V
|
T
|
K
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P
|
L
|
T
|
130 |
ICL2 | TM4 | ||||||||
Y
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P
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V
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K
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R
|
T
|
T
|
K
|
M
|
A
|
140 |
G
|
M
|
M
|
I
|
A
|
A
|
A
|
W
|
V
|
L
|
150 |
S
|
F
|
I
|
L
|
W
|
A
|
P
|
A
|
I
|
L
|
160 |
F
|
W
|
Q
|
F
|
I
|
V
|
G
|
V
|
R
|
T
|
170 |
ECL2 | |||||||||
V
|
E
|
D
|
G
|
E
|
C
|
Y
|
I
|
Q
|
F
|
180 |
TM5 | |||||||||
F
|
S
|
N
|
A
|
A
|
V
|
T
|
F
|
G
|
T
|
190 |
A
|
I
|
A
|
A
|
F
|
Y
|
L
|
P
|
V
|
I
|
200 |
I
|
M
|
T
|
V
|
L
|
Y
|
W
|
H
|
I
|
S
|
210 |
R
|
A
|
S
|
K
|
S
|
R
|
I
|
K
|
K
|
D
|
220 |
ICL3 | |||||||||
K
|
K
|
E
|
P
|
V
|
A
|
N
|
Q
|
D
|
P
|
230 |
|
|
|
|
|
|
|
|
||
V
|
S
|
P
|
S
|
L
|
V
|
Q
|
G
|
R
|
I
|
240 |
|
|
|
|
|
|
|
|
|
|
V
|
K
|
P
|
N
|
N
|
N
|
N
|
M
|
P
|
S
|
250 |
|
|
|
|
|
|
|
|
|
|
S
|
D
|
D
|
G
|
L
|
E
|
H
|
N
|
K
|
I
|
260 |
|
|
|
|
|
|
|
|
|
|
Q
|
N
|
G
|
K
|
A
|
P
|
R
|
D
|
P
|
V
|
270 |
|
|
|
|
|
|
|
|
|
|
T
|
E
|
N
|
C
|
V
|
Q
|
G
|
E
|
E
|
K
|
280 |
|
|
|
|
|
|
|
|
|
|
E
|
S
|
S
|
N
|
D
|
S
|
T
|
S
|
V
|
S
|
290 |
|
|
|
|
|
|
|
|
|
|
A
|
V
|
A
|
S
|
N
|
M
|
R
|
D
|
D
|
E
|
300 |
|
|
|
|
|
|
|
|
|
|
I
|
T
|
Q
|
D
|
E
|
N
|
T
|
V
|
S
|
T
|
310 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
G
|
H
|
S
|
K
|
D
|
E
|
N
|
S
|
320 |
|
|
|
|
|
|
|
|
|
|
K
|
Q
|
T
|
C
|
I
|
R
|
I
|
G
|
T
|
K
|
330 |
|
|
|
|
|
|
|
|
|
|
T
|
P
|
K
|
S
|
D
|
S
|
C
|
T
|
P
|
T
|
340 |
|
|
|
|
|
|
|
|
|
|
N
|
T
|
T
|
V
|
E
|
V
|
V
|
G
|
S
|
S
|
350 |
|
|
|
|
|
|
|
|
|
|
G
|
Q
|
N
|
G
|
D
|
E
|
K
|
Q
|
N
|
I
|
360 |
V
|
A
|
R
|
K
|
I
|
V
|
K
|
M
|
T
|
K
|
370 |
TM6 | |||||||||
Q
|
P
|
A
|
K
|
K
|
K
|
P
|
P
|
P
|
S
|
380 |
R
|
E
|
K
|
K
|
V
|
T
|
R
|
T
|
I
|
L
|
390 |
A
|
I
|
L
|
L
|
A
|
F
|
I
|
I
|
T
|
W
|
400 |
A
|
P
|
Y
|
N
|
V
|
M
|
V
|
L
|
I
|
N
|
410 |
ECL3 | TM7 | ||||||||
T
|
F
|
C
|
A
|
P
|
C
|
I
|
P
|
N
|
T
|
420 |
V
|
W
|
T
|
I
|
G
|
Y
|
W
|
L
|
C
|
Y
|
430 |
I
|
N
|
S
|
T
|
I
|
N
|
P
|
A
|
C
|
Y
|
440 |
H8 | |||||||||
A
|
L
|
C
|
N
|
A
|
T
|
F
|
K
|
K
|
T
|
450 |
F
|
K
|
H
|
L
|
L
|
M
|
C
|
H
|
Y
|
K
|
460 |
C-term | |||||
N
|
I
|
G
|
A
|
T
|
R
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|
|
|
|
|
|
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Expression | ||||||||||
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||||||
N-term | |||||||||
|
D
|
S
|
T
|
D
|
S
|
S
|
D
|
N
|
S
|
11 |
TM1 | |||||||||
L
|
A
|
L
|
T
|
S
|
P
|
Y
|
K
|
T
|
F
|
21 |
E
|
V
|
V
|
F
|
I
|
V
|
L
|
V
|
A
|
G
|
31 |
S
|
L
|
S
|
L
|
V
|
T
|
I
|
I
|
G
|
N
|
41 |
I
|
L
|
V
|
M
|
V
|
S
|
I
|
K
|
V
|
N
|
51 |
ICL1 | TM2 | ||||||||
R
|
H
|
L
|
Q
|
T
|
V
|
N
|
N
|
Y
|
F
|
61 |
L
|
F
|
S
|
L
|
A
|
C
|
A
|
D
|
L
|
I
|
71 |
I
|
G
|
V
|
F
|
S
|
M
|
N
|
L
|
Y
|
T
|
81 |
ECL1 | |||||||||
L
|
Y
|
T
|
V
|
I
|
G
|
Y
|
W
|
P
|
L
|
91 |
TM3 | |||||||||
G
|
P
|
V
|
V
|
C
|
D
|
L
|
W
|
L
|
A
|
101 |
L
|
D
|
Y
|
V
|
V
|
S
|
N
|
A
|
S
|
V
|
111 |
M
|
N
|
L
|
L
|
I
|
I
|
S
|
F
|
D
|
R
|
121 |
ICL2 | |||||||||
Y
|
F
|
C
|
V
|
T
|
K
|
P
|
L
|
T
|
Y
|
131 |
TM4 | |||||||||
P
|
V
|
K
|
R
|
T
|
T
|
K
|
M
|
A
|
G
|
141 |
M
|
M
|
I
|
A
|
A
|
A
|
W
|
V
|
L
|
S
|
151 |
F
|
I
|
L
|
W
|
A
|
P
|
A
|
I
|
L
|
F
|
161 |
ECL2 | |||||||||
W
|
Q
|
F
|
I
|
V
|
G
|
V
|
R
|
T
|
V
|
171 |
E
|
D
|
G
|
E
|
C
|
Y
|
I
|
Q
|
F
|
F
|
181 |
TM5 | |||||||||
S
|
N
|
A
|
A
|
V
|
T
|
F
|
G
|
T
|
A
|
191 |
I
|
A
|
A
|
F
|
Y
|
L
|
P
|
V
|
I
|
I
|
201 |
M
|
T
|
V
|
L
|
Y
|
W
|
H
|
I
|
S
|
R
|
211 |
A
|
S
|
K
|
S
|
R
|
I
|
K
|
K
|
D
|
K
|
221 |
ICL3 | |||||||||
K
|
E
|
P
|
V
|
A
|
N
|
Q
|
D
|
P
|
V
|
231 |
|
|||||||||
S
|
|
I
|
V
|
A
|
R
|
K
|
I
|
V
|
K
|
367 |
M
|
T
|
K
|
Q
|
P
|
A
|
K
|
K
|
K
|
P
|
377 |
TM6 | |||||||||
P
|
P
|
S
|
R
|
E
|
K
|
K
|
V
|
T
|
R
|
387 |
T
|
I
|
L
|
A
|
I
|
L
|
L
|
A
|
F
|
I
|
397 |
I
|
T
|
W
|
A
|
P
|
Y
|
N
|
V
|
M
|
V
|
407 |
ECL3 | |||||||||
L
|
I
|
N
|
T
|
F
|
C
|
A
|
P
|
C
|
I
|
417 |
TM7 | |||||||||
P
|
N
|
T
|
V
|
W
|
T
|
I
|
G
|
Y
|
W
|
427 |
L
|
C
|
Y
|
I
|
N
|
S
|
T
|
I
|
N
|
P
|
437 |
H8 | |||||||||
A
|
C
|
Y
|
A
|
L
|
C
|
N
|
A
|
T
|
F
|
447 |
K
|
K
|
T
|
F
|
K
|
H
|
L
|
L
|
M
|
C
|
457 |
C-term | ||||||||
H
|
Y
|
K
|
N
|
I
|
G
|
A
|
T
|
R
|
466 |
|
|
|
|
|
|
|
|
|
|
|
Expression | ||||||||||
|
|
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|
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SCHEMATIC
Perspective:
Wild-type
Construct
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
3 | | N | D |
6 | | N | D |
9 | | N | D |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: auto
Name: Expression tag
Sequence: None
Deletions:
Positions: C-term_1 None:None
Presence in Crystal:YES
Remarks: None
Protein_type: auto
Name: Expression tag
Sequence: None
Deletions:
Positions: N-term_0 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|