ste2_yeast
NAME
7AD3
ORGANISM
strain ATCC 204508 / S288c (Saccharomyces cerevisiae)
PROTEIN NAME
ste2_yeast
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
S
|
D
|
A
|
A
|
P
|
S
|
L
|
S
|
N
|
10 |
|
|
|
|
|
|
|
|||
L
|
F
|
Y
|
D
|
P
|
T
|
Y
|
N
|
P
|
G
|
20 |
|
|
|
|
|
|
|
|
|
|
D1S1 | |||||||||
Q
|
S
|
T
|
I
|
N
|
Y
|
T
|
S
|
I
|
Y
|
30 |
|
|
|
|
|
|
|
|
|
|
D1S2 | TM1 | ||||||||
G
|
N
|
G
|
S
|
T
|
I
|
T
|
F
|
D
|
E
|
40 |
|
|
|
|
|
|
|
|
|
|
L
|
Q
|
G
|
L
|
V
|
N
|
S
|
T
|
V
|
T
|
50 |
|
|
|
|
|
|
|
|
|
|
Q
|
A
|
I
|
M
|
F
|
G
|
V
|
R
|
C
|
G
|
60 |
|
|
|
|
|
|
|
|
|
|
A
|
A
|
A
|
L
|
T
|
L
|
I
|
V
|
M
|
W
|
70 |
|
|
|
|
|
|
|
|
|
|
ICL1 | TM2 | ||||||||
M
|
T
|
S
|
R
|
S
|
R
|
K
|
T
|
P
|
I
|
80 |
|
|
|
|
|
|
|
|
|
|
F
|
I
|
I
|
N
|
Q
|
V
|
S
|
L
|
F
|
L
|
90 |
|
|
|
|
|
|
|
|
|
|
I
|
I
|
L
|
H
|
S
|
A
|
L
|
Y
|
F
|
K
|
100 |
|
|
|
|
|
|
|
|
|
|
D1e1 | |||||||||
Y
|
L
|
L
|
S
|
N
|
Y
|
S
|
S
|
V
|
T
|
110 |
|
|
|
|
|
|
|
|
|
|
Y
|
A
|
L
|
T
|
G
|
F
|
P
|
Q
|
F
|
I
|
120 |
|
|
|
|
|
|
|
|
|
|
TM3 | |||||||||
S
|
R
|
G
|
D
|
V
|
H
|
V
|
Y
|
G
|
A
|
130 |
|
|
|
|
|
|
|
|
|
|
T
|
N
|
I
|
I
|
Q
|
V
|
L
|
L
|
V
|
A
|
140 |
|
|
|
|
|
|
|
|
|
|
S
|
I
|
E
|
T
|
S
|
L
|
V
|
F
|
Q
|
I
|
150 |
|
|
|
|
|
|
|
|
|
|
ICL2 | |||||||||
K
|
V
|
I
|
F
|
T
|
G
|
D
|
N
|
F
|
K
|
160 |
|
|
|
|
|
|
|
|
|
|
TM4 | |||||||||
R
|
I
|
G
|
L
|
M
|
L
|
T
|
S
|
I
|
S
|
170 |
|
|
|
|
|
|
|
|
|
|
F
|
T
|
L
|
G
|
I
|
A
|
T
|
V
|
T
|
M
|
180 |
|
|
|
|
|
|
|
|
|
|
Y
|
F
|
V
|
S
|
A
|
V
|
K
|
G
|
M
|
I
|
190 |
|
|
|
|
|
|
|
|
|
|
ECL2 | |||||||||
V
|
T
|
Y
|
N
|
D
|
V
|
S
|
A
|
T
|
Q
|
200 |
|
|
|
|
|
|
|
|
|
|
TM5 | |||||||||
D
|
K
|
Y
|
F
|
N
|
A
|
S
|
T
|
I
|
L
|
210 |
|
|
|
|
|
|
|
|
|
|
L
|
A
|
S
|
S
|
I
|
N
|
F
|
M
|
S
|
F
|
220 |
|
|
|
|
|
|
|
|
|
|
V
|
L
|
V
|
V
|
K
|
L
|
I
|
L
|
A
|
I
|
230 |
|
|
|
|
|
|
|
|
|
|
ICL3 | |||||||||
R
|
S
|
R
|
R
|
F
|
L
|
G
|
L
|
K
|
Q
|
240 |
|
|
|
|
|
|
|
|
|
|
TM6 | |||||||||
F
|
D
|
S
|
F
|
H
|
I
|
L
|
L
|
I
|
M
|
250 |
|
|
|
|
|
|
|
|
|
|
S
|
C
|
Q
|
S
|
L
|
L
|
V
|
P
|
S
|
I
|
260 |
|
|
|
|
|
|
|
|
|
|
I
|
F
|
I
|
L
|
A
|
Y
|
S
|
L
|
K
|
P
|
270 |
|
|
|
|
|
|
|
|
|
|
ECL3 | TM7 | ||||||||
N
|
Q
|
G
|
T
|
D
|
V
|
L
|
T
|
T
|
V
|
280 |
|
|
|
|
|
|
|
|
|
|
A
|
T
|
L
|
L
|
A
|
V
|
L
|
S
|
L
|
P
|
290 |
|
|
|
|
|
|
|
|
|
|
L
|
S
|
S
|
M
|
W
|
A
|
T
|
A
|
A
|
N
|
300 |
|
|
|
|
|
|
|
|
|
|
N
|
A
|
S
|
K
|
T
|
N
|
T
|
I
|
T
|
S
|
310 |
|
|
|
|
|
|
|
|
|
|
C-term | |||||||||
D
|
F
|
T
|
T
|
S
|
T
|
D
|
R
|
F
|
Y
|
320 |
|
|
|
|
|
|
|
|
|
|
P
|
G
|
T
|
L
|
S
|
S
|
F
|
Q
|
T
|
D
|
330 |
|
|
|
|
|
|
|
|
|
|
S
|
I
|
N
|
N
|
D
|
A
|
K
|
S
|
S
|
L
|
340 |
|
|
|
|
|
|
|
|
|
|
R
|
S
|
R
|
L
|
Y
|
D
|
L
|
Y
|
P
|
R
|
350 |
|
|
|
|
|
|
|
|
|
|
R
|
K
|
E
|
T
|
T
|
S
|
D
|
K
|
H
|
S
|
360 |
|
|
|
|
|
|
|
|
|
|
E
|
R
|
T
|
F
|
V
|
S
|
E
|
T
|
A
|
D
|
370 |
|
|
|
|
|
|
|
|
|
|
D
|
I
|
E
|
K
|
N
|
Q
|
F
|
Y
|
Q
|
L
|
380 |
|
|
|
|
|
|
|
|
|
|
P
|
T
|
P
|
T
|
S
|
S
|
K
|
N
|
T
|
R
|
390 |
|
|
|
|
|
|
|
|
|
|
I
|
G
|
P
|
F
|
A
|
D
|
A
|
S
|
Y
|
K
|
400 |
|
|
|
|
|
|
|
|
|
|
E
|
G
|
E
|
V
|
E
|
P
|
V
|
D
|
M
|
Y
|
410 |
|
|
|
|
|
|
|
|
|
|
T
|
P
|
D
|
T
|
A
|
A
|
D
|
E
|
E
|
A
|
420 |
|
|
|
|
|
|
|
|
|
|
R
|
K
|
F
|
W
|
T
|
E
|
D
|
N
|
N
|
N
|
430 |
|
L
|
N-term | |||||||||
M
|
S
|
D
|
A
|
A
|
P
|
S
|
L
|
S
|
N
|
10 |
|
|||
L
|
F
|
Y
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|