gpbar_human
NAME
7BW0
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
gpbar_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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|
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|||||
N-term | |||||||||
M
|
T
|
P
|
N
|
S
|
T
|
G
|
E
|
V
|
P
|
10 |
TM1 | |||||||||
S
|
P
|
I
|
P
|
K
|
G
|
A
|
L
|
G
|
L
|
20 |
S
|
L
|
A
|
L
|
A
|
S
|
L
|
I
|
I
|
T
|
30 |
A
|
N
|
L
|
L
|
L
|
A
|
L
|
G
|
I
|
A
|
40 |
ICL1 | TM2 | ||||||||
W
|
D
|
R
|
R
|
L
|
R
|
S
|
P
|
P
|
A
|
50 |
G
|
C
|
F
|
F
|
L
|
S
|
L
|
L
|
L
|
A
|
60 |
G
|
L
|
L
|
T
|
G
|
L
|
A
|
L
|
P
|
T
|
70 |
ECL1 | |||||||||
L
|
P
|
G
|
L
|
W
|
N
|
Q
|
S
|
R
|
R
|
80 |
TM3 | |||||||||
G
|
Y
|
W
|
S
|
C
|
L
|
L
|
V
|
Y
|
L
|
90 |
A
|
P
|
N
|
F
|
S
|
F
|
L
|
S
|
L
|
L
|
100 |
A
|
N
|
L
|
L
|
L
|
V
|
H
|
G
|
E
|
R
|
110 |
ICL2 | |||||||||
Y
|
M
|
A
|
V
|
L
|
R
|
P
|
L
|
Q
|
P
|
120 |
TM4 | |||||||||
P
|
G
|
S
|
I
|
R
|
L
|
A
|
L
|
L
|
L
|
130 |
T
|
W
|
A
|
G
|
P
|
L
|
L
|
F
|
A
|
S
|
140 |
ECL2 | |||||||||
L
|
P
|
A
|
L
|
G
|
W
|
N
|
H
|
W
|
T
|
150 |
P
|
G
|
A
|
N
|
C
|
S
|
S
|
Q
|
A
|
I
|
160 |
TM5 | |||||||||
F
|
P
|
A
|
P
|
Y
|
L
|
Y
|
L
|
E
|
V
|
170 |
Y
|
G
|
L
|
L
|
L
|
P
|
A
|
V
|
G
|
A
|
180 |
A
|
A
|
F
|
L
|
S
|
V
|
R
|
V
|
L
|
A
|
190 |
T
|
A
|
H
|
R
|
Q
|
L
|
Q
|
D
|
I
|
C
|
200 |
R
|
L
|
E
|
R
|
A
|
V
|
C
|
R
|
D
|
E
|
210 |
ICL3 | TM6 | ||||||||
P
|
S
|
A
|
L
|
A
|
R
|
A
|
L
|
T
|
W
|
220 |
R
|
Q
|
A
|
R
|
A
|
Q
|
A
|
G
|
A
|
M
|
230 |
L
|
L
|
F
|
G
|
L
|
C
|
W
|
G
|
P
|
Y
|
240 |
V
|
A
|
T
|
L
|
L
|
L
|
S
|
V
|
L
|
A
|
250 |
ECL3 | TM7 | ||||||||
Y
|
E
|
Q
|
R
|
P
|
P
|
L
|
G
|
P
|
G
|
260 |
T
|
L
|
L
|
S
|
L
|
L
|
S
|
L
|
G
|
S
|
270 |
A
|
S
|
A
|
A
|
A
|
V
|
P
|
V
|
A
|
M
|
280 |
H8 | |||||||||
G
|
L
|
G
|
D
|
Q
|
R
|
Y
|
T
|
A
|
P
|
290 |
W
|
R
|
A
|
A
|
A
|
Q
|
R
|
C
|
L
|
Q
|
300 |
|
|
|
|||||||
C-term | |||||||||
G
|
L
|
W
|
G
|
R
|
A
|
S
|
R
|
D
|
S
|
310 |
|
|
|
|
|
|
|
|
|
|
P
|
G
|
P
|
S
|
I
|
A
|
Y
|
H
|
P
|
S
|
320 |
|
|
|
|
|
|
|
|
|
|
S
|
Q
|
S
|
S
|
V
|
D
|
L
|
D
|
L
|
N
|
330 |
|
|||||||||
N-term | |||||||||
|
T
|
G
|
E
|
V
|
P
|
S
|
P
|
I
|
P
|
14 |
TM1 | |||||||||
K
|
G
|
A
|
L
|
G
|
L
|
S
|
L
|
A
|
L
|
24 |
A
|
S
|
L
|
I
|
I
|
T
|
A
|
N
|
L
|
L
|
34 |
L
|
A
|
L
|
G
|
I
|
A
|
W
|
D
|
R
|
R
|
44 |
ICL1 | TM2 | ||||||||
L
|
R
|
S
|
P
|
P
|
A
|
G
|
C
|
F
|
F
|
54 |
L
|
S
|
L
|
L
|
L
|
A
|
G
|
L
|
L
|
T
|
64 |
G
|
L
|
A
|
L
|
P
|
T
|
L
|
P
|
G
|
L
|
74 |
ECL1 | TM3 | ||||||||
W
|
N
|
Q
|
S
|
R
|
R
|
G
|
Y
|
W
|
S
|
84 |
C
|
L
|
L
|
V
|
Y
|
L
|
A
|
P
|
N
|
F
|
94 |
S
|
F
|
L
|
S
|
L
|
L
|
A
|
N
|
L
|
L
|
104 |
L
|
V
|
H
|
G
|
E
|
R
|
Y
|
M
|
A
|
V
|
114 |
ICL2 | |||||||||
L
|
R
|
P
|
L
|
Q
|
P
|
P
|
G
|
S
|
I
|
124 |
TM4 | |||||||||
R
|
L
|
A
|
L
|
L
|
L
|
T
|
W
|
A
|
G
|
134 |
P
|
L
|
L
|
F
|
A
|
S
|
L
|
P
|
A
|
L
|
144 |
ECL2 | |||||||||
G
|
W
|
N
|
H
|
W
|
T
|
P
|
G
|
A
|
N
|
154 |
TM5 | |||||||||
C
|
S
|
S
|
Q
|
A
|
I
|
F
|
P
|
A
|
P
|
164 |
Y
|
L
|
Y
|
L
|
E
|
V
|
Y
|
G
|
L
|
L
|
174 |
L
|
P
|
A
|
V
|
G
|
A
|
A
|
A
|
F
|
L
|
184 |
S
|
V
|
R
|
V
|
L
|
A
|
T
|
A
|
H
|
R
|
194 |
Q
|
L
|
Q
|
D
|
I
|
C
|
R
|
L
|
E
|
R
|
204 |
ICL3 | TM6 | ||||||||
A
|
V
|
C
|
R
|
D
|
E
|
P
|
S
|
A
|
L
|
214 |
A
|
R
|
A
|
L
|
T
|
W
|
R
|
Q
|
A
|
R
|
224 |
A
|
Q
|
A
|
G
|
A
|
M
|
L
|
L
|
F
|
G
|
234 |
L
|
C
|
W
|
G
|
P
|
Y
|
V
|
A
|
T
|
L
|
244 |
L
|
L
|
S
|
V
|
L
|
A
|
Y
|
E
|
Q
|
R
|
254 |
ECL3 | TM7 | ||||||||
P
|
P
|
L
|
G
|
P
|
G
|
T
|
L
|
L
|
S
|
264 |
L
|
L
|
S
|
L
|
G
|
S
|
A
|
S
|
A
|
A
|
274 |
A
|
V
|
P
|
V
|
A
|
M
|
G
|
L
|
G
|
D
|
284 |
H8 | |||||||||
Q
|
R
|
Y
|
T
|
A
|
P
|
W
|
R
|
A
|
A
|
294 |
C-term | |||||||||
A
|
Q
|
R
|
C
|
L
|
Q
|
G
|
L
|
W
|
G
|
304 |
|
|||
R
|
A
|
S
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|