5ht1b_human
NAME
7C61
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
5ht1b_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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E
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E
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P
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G
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A
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Q
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C
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A
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P
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10 |
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|
|
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P
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P
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P
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A
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G
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S
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E
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T
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W
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V
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20 |
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|
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P
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Q
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A
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N
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L
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S
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S
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A
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P
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S
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30 |
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||||||||
Q
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N
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C
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S
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A
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K
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D
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Y
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I
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Y
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40 |
TM1 | |||||||||
Q
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D
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S
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I
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S
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L
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P
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W
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K
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V
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50 |
L
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L
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V
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M
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L
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L
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A
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L
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I
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T
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60 |
L
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A
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T
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T
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L
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S
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N
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A
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F
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V
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70 |
I
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A
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T
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V
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Y
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R
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T
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R
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K
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L
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80 |
ICL1 | TM2 | ||||||||
H
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T
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P
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A
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N
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Y
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L
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I
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A
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S
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90 |
L
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A
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V
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T
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D
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L
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L
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V
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S
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I
|
100 |
L
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V
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M
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P
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I
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S
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T
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M
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Y
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T
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110 |
ECL1 | TM3 | ||||||||
V
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T
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G
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R
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W
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T
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L
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G
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Q
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V
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120 |
V
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C
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D
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F
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W
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L
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S
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S
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D
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I
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130 |
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|||||||||
T
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C
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C
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T
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A
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S
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I
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L
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H
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L
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140 |
C
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V
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I
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A
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L
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D
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R
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Y
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W
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A
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150 |
ICL2 | |||||||||
I
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T
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D
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A
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V
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E
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Y
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S
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A
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K
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160 |
TM4 | |||||||||
R
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T
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P
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K
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R
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A
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A
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V
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M
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I
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170 |
A
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L
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V
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W
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V
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F
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S
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I
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S
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I
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180 |
S
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L
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P
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P
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F
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F
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W
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R
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Q
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A
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190 |
ECL2 | |||||||||
K
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A
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E
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E
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E
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V
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S
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E
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C
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V
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200 |
TM5 | |||||||||
V
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N
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T
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D
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H
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I
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L
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Y
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T
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V
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210 |
Y
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S
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T
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V
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G
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A
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F
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Y
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F
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P
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220 |
T
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L
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L
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L
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I
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A
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L
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Y
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G
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R
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230 |
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|||||||||
I
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Y
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V
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E
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A
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R
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S
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R
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I
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L
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240 |
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ICL3 | |||||||||
K
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Q
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T
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P
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N
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R
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T
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G
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K
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R
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250 |
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|
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L
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T
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R
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A
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Q
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L
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I
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T
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D
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S
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260 |
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P
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G
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S
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T
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S
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S
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V
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T
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S
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I
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270 |
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N
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S
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R
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V
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P
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D
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V
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P
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S
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E
|
280 |
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S
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G
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S
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P
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V
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Y
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V
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N
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Q
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V
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290 |
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K
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V
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R
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V
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S
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D
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A
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L
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L
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E
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300 |
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|||||
TM6 | |||||||||
K
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K
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K
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L
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M
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A
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A
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R
|
E
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R
|
310 |
K
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A
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T
|
K
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T
|
L
|
G
|
I
|
I
|
L
|
320 |
G
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A
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F
|
I
|
V
|
C
|
W
|
L
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P
|
F
|
330 |
F
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I
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I
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S
|
L
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V
|
M
|
P
|
I
|
C
|
340 |
ECL3 | TM7 | ||||||||
K
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D
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A
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C
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W
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F
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H
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L
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A
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I
|
350 |
F
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D
|
F
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F
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T
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W
|
L
|
G
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Y
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L
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360 |
N
|
S
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L
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I
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N
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P
|
I
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I
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Y
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T
|
370 |
H8 | |||||||||
M
|
S
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N
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E
|
D
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F
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K
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Q
|
A
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F
|
380 |
C-term | |||||||||
H
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K
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L
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I
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R
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F
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K
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C
|
T
|
S
|
390 |
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|||||||||
N-term | |||||||||
|
C
|
S
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A
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K
|
D
|
Y
|
I
|
Y
|
Q
|
41 |
TM1 | |||||||||
D
|
S
|
I
|
S
|
L
|
P
|
W
|
K
|
V
|
L
|
51 |
L
|
V
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M
|
L
|
L
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A
|
L
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I
|
T
|
L
|
61 |
A
|
T
|
T
|
L
|
S
|
N
|
A
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F
|
V
|
I
|
71 |
ICL1 | |||||||||
A
|
T
|
V
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Y
|
R
|
T
|
R
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K
|
L
|
H
|
81 |
TM2 | |||||||||
T
|
P
|
A
|
N
|
Y
|
L
|
I
|
A
|
S
|
L
|
91 |
A
|
V
|
T
|
D
|
L
|
L
|
V
|
S
|
I
|
L
|
101 |
V
|
M
|
P
|
I
|
S
|
T
|
M
|
Y
|
T
|
V
|
111 |
ECL1 | TM3 | ||||||||
T
|
G
|
R
|
W
|
T
|
L
|
G
|
Q
|
V
|
V
|
121 |
C
|
D
|
F
|
W
|
L
|
S
|
S
|
D
|
I
|
T
|
131 |
|
|||||||||
C
|
C
|
T
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A
|
S
|
I
|
W
|
H
|
L
|
C
|
141 |
V
|
I
|
A
|
L
|
D
|
R
|
Y
|
W
|
A
|
I
|
151 |
ICL2 | |||||||||
T
|
D
|
A
|
V
|
E
|
Y
|
S
|
A
|
K
|
R
|
161 |
TM4 | |||||||||
T
|
P
|
K
|
R
|
A
|
A
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V
|
M
|
I
|
A
|
171 |
L
|
V
|
W
|
V
|
F
|
S
|
I
|
S
|
I
|
S
|
181 |
ECL2 | |||||||||
L
|
P
|
P
|
F
|
F
|
W
|
R
|
Q
|
A
|
K
|
191 |
A
|
E
|
E
|
E
|
V
|
S
|
E
|
C
|
V
|
V
|
201 |
TM5 | |||||||||
N
|
T
|
D
|
H
|
I
|
L
|
Y
|
T
|
V
|
Y
|
211 |
S
|
T
|
V
|
G
|
A
|
F
|
Y
|
F
|
P
|
T
|
221 |
L
|
L
|
L
|
I
|
A
|
L
|
Y
|
G
|
R
|
I
|
231 |
|
|||||||||
Y
|
V
|
E
|
A
|
R
|
S
|
R
|
I
|
|
A
|
306 |
TM6 | |||||||||
A
|
R
|
E
|
R
|
K
|
A
|
T
|
K
|
T
|
L
|
316 |
G
|
I
|
I
|
L
|
G
|
A
|
F
|
I
|
V
|
C
|
326 |
W
|
L
|
P
|
F
|
F
|
I
|
I
|
S
|
L
|
V
|
336 |
ECL3 | |||||||||
M
|
P
|
I
|
C
|
K
|
D
|
A
|
C
|
W
|
F
|
346 |
TM7 | |||||||||
H
|
L
|
A
|
I
|
F
|
D
|
F
|
F
|
T
|
W
|
356 |
L
|
G
|
Y
|
L
|
N
|
S
|
L
|
I
|
N
|
P
|
366 |
H8 | |||||||||
I
|
I
|
Y
|
T
|
M
|
S
|
N
|
E
|
D
|
F
|
376 |
K
|
Q
|
A
|
F
|
H
|
K
|
L
|
I
|
R
|
F
|
386 |
C-term | |||||
K
|
C
|
T
|
S
|
||
390 |
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
138 | | L | W |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|