cckar_human
NAME
7F8Y
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
cckar_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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D
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V
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V
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D
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S
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L
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L
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V
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N
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10 |
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|
|
|
|
|
|
|
|
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G
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S
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N
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I
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T
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P
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P
|
C
|
E
|
L
|
20 |
|
|
|
|
|
|
|
|
|
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G
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L
|
E
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N
|
E
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T
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L
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F
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C
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L
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30 |
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||||
D
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Q
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P
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R
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P
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S
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K
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E
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W
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Q
|
40 |
TM1 | |||||||||
P
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A
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V
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Q
|
I
|
L
|
L
|
Y
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S
|
L
|
50 |
I
|
F
|
L
|
L
|
S
|
V
|
L
|
G
|
N
|
T
|
60 |
L
|
V
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I
|
T
|
V
|
L
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I
|
R
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N
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K
|
70 |
ICL1 | TM2 | ||||||||
R
|
M
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R
|
T
|
V
|
T
|
N
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I
|
F
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L
|
80 |
L
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S
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L
|
A
|
V
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S
|
D
|
L
|
M
|
L
|
90 |
C
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L
|
F
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C
|
M
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P
|
F
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N
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L
|
I
|
100 |
ECL1 | |||||||||
P
|
N
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L
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L
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K
|
D
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F
|
I
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F
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G
|
110 |
TM3 | |||||||||
S
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A
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V
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C
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K
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T
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T
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T
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Y
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F
|
120 |
M
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G
|
T
|
S
|
V
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S
|
V
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S
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T
|
F
|
130 |
N
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L
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V
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A
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I
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S
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L
|
E
|
R
|
Y
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140 |
ICL2 | |||||||||
G
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A
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I
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C
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K
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P
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L
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Q
|
S
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R
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150 |
TM4 | |||||||||
V
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W
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Q
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T
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K
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S
|
H
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A
|
L
|
K
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160 |
V
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I
|
A
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A
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T
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W
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C
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L
|
S
|
F
|
170 |
T
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I
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M
|
T
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P
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Y
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P
|
I
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Y
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S
|
180 |
ECL2 | |||||||||
N
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L
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V
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P
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F
|
T
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K
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N
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N
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N
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190 |
Q
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T
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A
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N
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M
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C
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R
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F
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L
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L
|
200 |
TM5 | |||||||||
P
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N
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D
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V
|
M
|
Q
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Q
|
S
|
W
|
H
|
210 |
T
|
F
|
L
|
L
|
L
|
I
|
L
|
F
|
L
|
I
|
220 |
P
|
G
|
I
|
V
|
M
|
M
|
V
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A
|
Y
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G
|
230 |
L
|
I
|
S
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L
|
E
|
L
|
Y
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Q
|
G
|
I
|
240 |
|
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|
|
|
|
|
|
|
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ICL3 | |||||||||
K
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F
|
E
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A
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S
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Q
|
K
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K
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S
|
A
|
250 |
|
|
|
|
|
|
|
|
|
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K
|
E
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R
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K
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P
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S
|
T
|
T
|
S
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S
|
260 |
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|
|
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|
|
|
|
|
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G
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K
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Y
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E
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D
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S
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D
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G
|
C
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Y
|
270 |
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|
|
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L
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Q
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K
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T
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R
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P
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P
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R
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K
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L
|
280 |
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|
|
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|
|
|
|
|
E
|
L
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R
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Q
|
L
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S
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T
|
G
|
S
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S
|
290 |
|
|
|
|
|
|
|
|
|
|
S
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R
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A
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N
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R
|
I
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R
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S
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N
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S
|
300 |
|
|||||||||
TM6 | |||||||||
S
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A
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A
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N
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L
|
M
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A
|
K
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K
|
R
|
310 |
V
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I
|
R
|
M
|
L
|
I
|
V
|
I
|
V
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V
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320 |
L
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F
|
F
|
L
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C
|
W
|
M
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P
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I
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F
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330 |
S
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A
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N
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A
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W
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R
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A
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Y
|
D
|
T
|
340 |
ECL3 | |||||||||
A
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S
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A
|
E
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R
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R
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L
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S
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G
|
T
|
350 |
TM7 | |||||||||
P
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I
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S
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F
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I
|
L
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L
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L
|
S
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Y
|
360 |
T
|
S
|
S
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C
|
V
|
N
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P
|
I
|
I
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Y
|
370 |
H8 | |||||||||
C
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F
|
M
|
N
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K
|
R
|
F
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R
|
L
|
G
|
380 |
F
|
M
|
A
|
T
|
F
|
P
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C
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C
|
P
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N
|
390 |
C-term | |||||||||
P
|
G
|
P
|
P
|
G
|
A
|
R
|
G
|
E
|
V
|
400 |
|
|
|
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||||||
G
|
E
|
E
|
E
|
E
|
G
|
G
|
T
|
T
|
G
|
410 |
|
|
|
|
|
|
|
|
|
|
A
|
S
|
L
|
S
|
R
|
F
|
S
|
Y
|
S
|
H
|
420 |
|
|
|
|
|
|
|
|
M
|
S
|
A
|
S
|
V
|
P
|
P
|
Q
|
|
|||||||||
N-term | TM1 | ||||||||
|
K
|
E
|
W
|
Q
|
P
|
A
|
V
|
Q
|
I
|
45 |
L
|
L
|
Y
|
S
|
L
|
I
|
F
|
L
|
L
|
S
|
55 |
V
|
L
|
G
|
N
|
T
|
L
|
V
|
I
|
T
|
V
|
65 |
ICL1 | |||||||||
L
|
I
|
R
|
N
|
K
|
R
|
M
|
R
|
T
|
V
|
75 |
TM2 | |||||||||
T
|
N
|
I
|
F
|
L
|
L
|
S
|
L
|
A
|
V
|
85 |
S
|
D
|
L
|
M
|
L
|
C
|
L
|
F
|
C
|
M
|
95 |
P
|
F
|
N
|
L
|
I
|
P
|
N
|
L
|
L
|
K
|
105 |
ECL1 | TM3 | ||||||||
D
|
F
|
I
|
F
|
G
|
S
|
A
|
V
|
C
|
K
|
115 |
T
|
T
|
T
|
Y
|
F
|
M
|
G
|
T
|
S
|
V
|
125 |
S
|
V
|
S
|
T
|
F
|
N
|
L
|
V
|
A
|
I
|
135 |
S
|
L
|
E
|
R
|
Y
|
G
|
A
|
I
|
C
|
K
|
145 |
ICL2 | |||||||||
P
|
L
|
Q
|
S
|
R
|
V
|
W
|
Q
|
T
|
K
|
155 |
TM4 | |||||||||
S
|
H
|
A
|
L
|
K
|
V
|
I
|
A
|
A
|
T
|
165 |
W
|
C
|
L
|
S
|
F
|
T
|
I
|
M
|
T
|
P
|
175 |
ECL2 | |||||||||
Y
|
P
|
I
|
Y
|
S
|
N
|
L
|
V
|
P
|
F
|
185 |
T
|
K
|
N
|
N
|
N
|
Q
|
T
|
A
|
N
|
M
|
195 |
TM5 | |||||||||
C
|
R
|
F
|
L
|
L
|
P
|
N
|
D
|
V
|
M
|
205 |
Q
|
Q
|
S
|
W
|
H
|
T
|
F
|
L
|
L
|
L
|
215 |
I
|
L
|
F
|
L
|
I
|
P
|
G
|
I
|
V
|
M
|
225 |
M
|
V
|
A
|
Y
|
G
|
L
|
I
|
S
|
L
|
E
|
235 |
|
|||||||||
TM6 | |||||||||
L
|
Y
|
Q
|
G
|
I
|
|
A
|
A
|
N
|
L
|
305 |
M
|
A
|
K
|
K
|
R
|
V
|
I
|
R
|
M
|
L
|
315 |
I
|
V
|
I
|
V
|
V
|
L
|
F
|
F
|
L
|
C
|
325 |
W
|
M
|
P
|
I
|
F
|
S
|
A
|
N
|
A
|
W
|
335 |
ECL3 | |||||||||
R
|
A
|
Y
|
D
|
T
|
A
|
S
|
A
|
E
|
R
|
345 |
TM7 | |||||||||
R
|
L
|
S
|
G
|
T
|
P
|
I
|
S
|
F
|
I
|
355 |
L
|
L
|
L
|
S
|
Y
|
T
|
S
|
S
|
C
|
V
|
365 |
H8 | |||||||||
N
|
P
|
I
|
I
|
Y
|
C
|
F
|
M
|
N
|
K
|
375 |
R
|
F
|
R
|
L
|
G
|
F
|
M
|
A
|
T
|
F
|
385 |
P
|
C
|
C
|
P
|
N
|
P
|
G
|
P
|
P
|
G
|
395 |
C-term | |||||||||
A
|
R
|
G
|
E
|
V
|
G
|
E
|
E
|
E
|
E
|
405 |
|
|
G
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|