grm5_human
NAME
7FD8
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
grm5_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
|
V
|
L
|
L
|
L
|
I
|
L
|
S
|
V
|
L
|
10 |
|
|
|
|
|
|
|
|
|
|
L
|
L
|
K
|
E
|
D
|
V
|
R
|
G
|
S
|
A
|
20 |
Q
|
S
|
S
|
E
|
R
|
R
|
V
|
V
|
A
|
H
|
30 |
M
|
P
|
G
|
D
|
I
|
I
|
I
|
G
|
A
|
L
|
40 |
F
|
S
|
V
|
H
|
H
|
Q
|
P
|
T
|
V
|
D
|
50 |
K
|
V
|
H
|
E
|
R
|
K
|
C
|
G
|
A
|
V
|
60 |
R
|
E
|
Q
|
Y
|
G
|
I
|
Q
|
R
|
V
|
E
|
70 |
A
|
M
|
L
|
H
|
T
|
L
|
E
|
R
|
I
|
N
|
80 |
S
|
D
|
P
|
T
|
L
|
L
|
P
|
N
|
I
|
T
|
90 |
L
|
G
|
C
|
E
|
I
|
R
|
D
|
S
|
C
|
W
|
100 |
H
|
S
|
A
|
V
|
A
|
L
|
E
|
Q
|
S
|
I
|
110 |
E
|
F
|
I
|
R
|
D
|
S
|
L
|
I
|
S
|
S
|
120 |
E
|
E
|
E
|
E
|
G
|
L
|
V
|
R
|
C
|
V
|
130 |
D
|
G
|
S
|
S
|
S
|
S
|
F
|
R
|
S
|
K
|
140 |
K
|
P
|
I
|
V
|
G
|
V
|
I
|
G
|
P
|
G
|
150 |
S
|
S
|
S
|
V
|
A
|
I
|
Q
|
V
|
Q
|
N
|
160 |
L
|
L
|
Q
|
L
|
F
|
N
|
I
|
P
|
Q
|
I
|
170 |
A
|
Y
|
S
|
A
|
T
|
S
|
M
|
D
|
L
|
S
|
180 |
D
|
K
|
T
|
L
|
F
|
K
|
Y
|
F
|
M
|
R
|
190 |
V
|
V
|
P
|
S
|
D
|
A
|
Q
|
Q
|
A
|
R
|
200 |
A
|
M
|
V
|
D
|
I
|
V
|
K
|
R
|
Y
|
N
|
210 |
W
|
T
|
Y
|
V
|
S
|
A
|
V
|
H
|
T
|
E
|
220 |
G
|
N
|
Y
|
G
|
E
|
S
|
G
|
M
|
E
|
A
|
230 |
F
|
K
|
D
|
M
|
S
|
A
|
K
|
E
|
G
|
I
|
240 |
C
|
I
|
A
|
H
|
S
|
Y
|
K
|
I
|
Y
|
S
|
250 |
N
|
A
|
G
|
E
|
Q
|
S
|
F
|
D
|
K
|
L
|
260 |
L
|
K
|
K
|
L
|
T
|
S
|
H
|
L
|
P
|
K
|
270 |
A
|
R
|
V
|
V
|
A
|
C
|
F
|
C
|
E
|
G
|
280 |
M
|
T
|
V
|
R
|
G
|
L
|
L
|
M
|
A
|
M
|
290 |
R
|
R
|
L
|
G
|
L
|
A
|
G
|
E
|
F
|
L
|
300 |
L
|
L
|
G
|
S
|
D
|
G
|
W
|
A
|
D
|
R
|
310 |
Y
|
D
|
V
|
T
|
D
|
G
|
Y
|
Q
|
R
|
E
|
320 |
A
|
V
|
G
|
G
|
I
|
T
|
I
|
K
|
L
|
Q
|
330 |
S
|
P
|
D
|
V
|
K
|
W
|
F
|
D
|
D
|
Y
|
340 |
Y
|
L
|
K
|
L
|
R
|
P
|
E
|
T
|
N
|
H
|
350 |
R
|
N
|
P
|
W
|
F
|
Q
|
E
|
F
|
W
|
Q
|
360 |
H
|
R
|
F
|
Q
|
C
|
R
|
L
|
E
|
G
|
F
|
370 |
P
|
Q
|
E
|
N
|
S
|
K
|
Y
|
N
|
K
|
T
|
380 |
C
|
N
|
S
|
S
|
L
|
T
|
L
|
K
|
T
|
H
|
390 |
H
|
V
|
Q
|
D
|
S
|
K
|
M
|
G
|
F
|
V
|
400 |
I
|
N
|
A
|
I
|
Y
|
S
|
M
|
A
|
Y
|
G
|
410 |
L
|
H
|
N
|
M
|
Q
|
M
|
S
|
L
|
C
|
P
|
420 |
G
|
Y
|
A
|
G
|
L
|
C
|
D
|
A
|
M
|
K
|
430 |
P
|
I
|
D
|
G
|
R
|
K
|
L
|
L
|
E
|
S
|
440 |
L
|
M
|
K
|
T
|
N
|
F
|
T
|
G
|
V
|
S
|
450 |
G
|
D
|
T
|
I
|
L
|
F
|
D
|
E
|
N
|
G
|
460 |
D
|
S
|
P
|
G
|
R
|
Y
|
E
|
I
|
M
|
N
|
470 |
F
|
K
|
E
|
M
|
G
|
K
|
D
|
Y
|
F
|
D
|
480 |
Y
|
I
|
N
|
V
|
G
|
S
|
W
|
D
|
N
|
G
|
490 |
E
|
L
|
K
|
M
|
D
|
D
|
D
|
E
|
V
|
W
|
500 |
S
|
K
|
K
|
S
|
N
|
I
|
I
|
R
|
S
|
V
|
510 |
C
|
S
|
E
|
P
|
C
|
E
|
K
|
G
|
Q
|
I
|
520 |
K
|
V
|
I
|
R
|
K
|
G
|
E
|
V
|
S
|
C
|
530 |
C
|
W
|
T
|
C
|
T
|
P
|
C
|
K
|
E
|
N
|
540 |
E
|
Y
|
V
|
F
|
D
|
E
|
Y
|
T
|
C
|
K
|
550 |
A
|
C
|
Q
|
L
|
G
|
S
|
W
|
P
|
T
|
D
|
560 |
D
|
L
|
T
|
G
|
C
|
D
|
L
|
I
|
P
|
V
|
570 |
TM1 | |||||||||
Q
|
Y
|
L
|
R
|
W
|
G
|
D
|
P
|
E
|
P
|
580 |
I
|
A
|
A
|
V
|
V
|
F
|
A
|
C
|
L
|
G
|
590 |
L
|
L
|
A
|
T
|
L
|
F
|
V
|
T
|
V
|
V
|
600 |
ICL1 | |||||||||
F
|
I
|
I
|
Y
|
R
|
D
|
T
|
P
|
V
|
V
|
610 |
TM2 | |||||||||
K
|
S
|
S
|
S
|
R
|
E
|
L
|
C
|
Y
|
I
|
620 |
I
|
L
|
A
|
G
|
I
|
C
|
L
|
G
|
Y
|
L
|
630 |
ECL1 | |||||||||
C
|
T
|
F
|
C
|
L
|
I
|
A
|
K
|
P
|
K
|
640 |
TM3 | |||||||||
Q
|
I
|
Y
|
C
|
Y
|
L
|
Q
|
R
|
I
|
G
|
650 |
I
|
G
|
L
|
S
|
P
|
A
|
M
|
S
|
Y
|
S
|
660 |
A
|
L
|
V
|
T
|
K
|
T
|
N
|
R
|
I
|
A
|
670 |
ICL2 | |||||||||
R
|
I
|
L
|
A
|
G
|
S
|
K
|
K
|
K
|
I
|
680 |
C
|
T
|
K
|
K
|
P
|
R
|
F
|
M
|
S
|
A
|
690 |
TM4 | |||||||||
C
|
A
|
Q
|
L
|
V
|
I
|
A
|
F
|
I
|
L
|
700 |
I
|
C
|
I
|
Q
|
L
|
G
|
I
|
I
|
V
|
A
|
710 |
ECL2 | |||||||||
L
|
F
|
I
|
M
|
E
|
P
|
P
|
D
|
I
|
M
|
720 |
H
|
D
|
Y
|
P
|
S
|
I
|
R
|
E
|
V
|
Y
|
730 |
TM5 | |||||||||
L
|
I
|
C
|
N
|
T
|
T
|
N
|
L
|
G
|
V
|
740 |
|
|||||||||
V
|
T
|
P
|
L
|
G
|
Y
|
N
|
G
|
L
|
L
|
750 |
|
|||||||||
I
|
L
|
S
|
C
|
T
|
F
|
Y
|
A
|
F
|
K
|
760 |
ICL3 | |||||||||
T
|
R
|
N
|
V
|
P
|
A
|
N
|
F
|
N
|
E
|
770 |
TM6 | |||||||||
A
|
K
|
Y
|
I
|
A
|
F
|
T
|
M
|
Y
|
T
|
780 |
T
|
C
|
I
|
I
|
W
|
L
|
A
|
F
|
V
|
P
|
790 |
ECL3 | TM7 | ||||||||||
I
|
Y
|
F
|
G
|
S
|
N
|
Y
|
K
|
I
|
I
|
||
800 |
T
|
M
|
C
|
F
|
S
|
V
|
S
|
L
|
S
|
A
|
810 |
T
|
V
|
A
|
L
|
G
|
C
|
M
|
F
|
V
|
P
|
820 |
H8 | |||||||||
K
|
V
|
Y
|
I
|
I
|
L
|
A
|
K
|
P
|
E
|
830 |
R
|
N
|
V
|
R
|
S
|
A
|
F
|
T
|
T
|
S
|
840 |
C-term | |||||||||
T
|
V
|
V
|
R
|
M
|
H
|
V
|
G
|
D
|
G
|
850 |
|
|
|
|
||||||
K
|
S
|
S
|
S
|
A
|
A
|
S
|
R
|
S
|
S
|
860 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
V
|
N
|
L
|
W
|
K
|
R
|
R
|
G
|
870 |
|
|
|
|
|
|
|
|
|
|
S
|
S
|
G
|
E
|
T
|
L
|
R
|
Y
|
K
|
D
|
880 |
|
|
|
|
|
|
|
|
|
|
R
|
R
|
L
|
A
|
Q
|
H
|
K
|
S
|
E
|
I
|
890 |
|
|
|
|
|
|
|
|
|
|
E
|
C
|
F
|
T
|
P
|
K
|
G
|
S
|
M
|
G
|
900 |
|
|
|
|
|
|
|
|
|
|
N
|
G
|
G
|
R
|
A
|
T
|
M
|
S
|
S
|
S
|
910 |
|
|
|
|
|
|
|
|
|
|
N
|
G
|
K
|
S
|
V
|
T
|
W
|
A
|
Q
|
N
|
920 |
|
|
|
|
|
|
|
|
|
|
E
|
K
|
S
|
S
|
R
|
G
|
Q
|
H
|
L
|
W
|
930 |
|
|
|
|
|
|
|
|
|
|
Q
|
R
|
L
|
S
|
I
|
H
|
I
|
N
|
K
|
K
|
940 |
|
|
|
|
|
|
|
|
|
|
E
|
N
|
P
|
N
|
Q
|
T
|
A
|
V
|
I
|
K
|
950 |
|
|
|
|
|
|
|
|
|
|
P
|
F
|
P
|
K
|
S
|
T
|
E
|
S
|
R
|
G
|
960 |
|
|
|
|
|
|
|
|
|
|
L
|
G
|
A
|
G
|
A
|
G
|
A
|
G
|
G
|
S
|
970 |
|
|
|
|
|
|
|
|
|
|
A
|
G
|
G
|
V
|
G
|
A
|
T
|
G
|
G
|
A
|
980 |
|
|
|
|
|
|
|
|
|
|
G
|
C
|
A
|
G
|
A
|
G
|
P
|
G
|
G
|
P
|
990 |
|
|
|
|
|
|
|
|
|
|
E
|
S
|
P
|
D
|
A
|
G
|
P
|
K
|
A
|
L
|
1000 |
|
|
|
|
|
|
|
|
|
|
Y
|
D
|
V
|
A
|
E
|
A
|
E
|
E
|
H
|
F
|
1010 |
|
|
|
|
|
|
|
|
|
|
P
|
A
|
P
|
A
|
R
|
P
|
R
|
S
|
P
|
S
|
1020 |
|
|
|
|
|
|
|
|
|
|
P
|
I
|
S
|
T
|
L
|
S
|
H
|
R
|
A
|
G
|
1030 |
|
|
|
|
|
|
|
|
|
|
S
|
A
|
S
|
R
|
T
|
D
|
D
|
D
|
V
|
P
|
1040 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
H
|
S
|
E
|
P
|
V
|
A
|
R
|
S
|
1050 |
|
|
|
|
|
|
|
|
|
|
S
|
S
|
S
|
Q
|
G
|
S
|
L
|
M
|
E
|
Q
|
1060 |
|
|
|
|
|
|
|
|
|
|
I
|
S
|
S
|
V
|
V
|
T
|
R
|
F
|
T
|
A
|
1070 |
|
|
|
|
|
|
|
|
|
|
N
|
I
|
S
|
E
|
L
|
N
|
S
|
M
|
M
|
L
|
1080 |
|
|
|
|
|
|
|
|
|
|
S
|
T
|
A
|
A
|
P
|
S
|
P
|
G
|
V
|
G
|
1090 |
|
|
|
|
|
|
|
|
|
|
A
|
P
|
L
|
C
|
S
|
S
|
Y
|
L
|
I
|
P
|
1100 |
|
|
|
|
|
|
|
|
|
|
K
|
E
|
I
|
Q
|
L
|
P
|
T
|
T
|
M
|
T
|
1110 |
|
|
|
|
|
|
|
|
|
|
T
|
F
|
A
|
E
|
I
|
Q
|
P
|
L
|
P
|
A
|
1120 |
|
|
|
|
|
|
|
|
|
|
I
|
E
|
V
|
T
|
G
|
G
|
A
|
Q
|
P
|
A
|
1130 |
|
|
|
|
|
|
|
|
|
|
A
|
G
|
A
|
Q
|
A
|
A
|
G
|
D
|
A
|
A
|
1140 |
|
|
|
|
|
|
|
|
|
|
R
|
E
|
S
|
P
|
A
|
A
|
G
|
P
|
E
|
A
|
1150 |
|
|
|
|
|
|
|
|
|
|
A
|
A
|
A
|
K
|
P
|
D
|
L
|
E
|
E
|
L
|
1160 |
|
|
|
|
|
|
|
|
|
|
V
|
A
|
L
|
T
|
P
|
P
|
S
|
P
|
F
|
R
|
1170 |
|
|
|
|
|
|
|
|
|
|
D
|
S
|
V
|
D
|
S
|
G
|
S
|
T
|
T
|
P
|
1180 |
|
|
|
|
|
|
|
|
|
|
N
|
S
|
P
|
V
|
S
|
E
|
S
|
A
|
L
|
C
|
1190 |
|
|
|
|
|
|
|
|
|
|
I
|
P
|
S
|
S
|
P
|
K
|
Y
|
D
|
T
|
L
|
1200 |
|
|
|
|
|
|
|
|
|
|
I
|
I
|
R
|
D
|
Y
|
T
|
Q
|
S
|
S
|
S
|
1210 |
|
|
S
|
L
|
|
|||||||||
N-term | |||||||||
|
Q
|
S
|
S
|
E
|
R
|
R
|
V
|
V
|
A
|
29 |
H
|
M
|
P
|
G
|
D
|
I
|
I
|
I
|
G
|
A
|
39 |
L
|
F
|
S
|
V
|
H
|
H
|
Q
|
P
|
T
|
V
|
49 |
D
|
K
|
V
|
H
|
E
|
R
|
K
|
C
|
G
|
A
|
59 |
V
|
R
|
E
|
Q
|
Y
|
G
|
I
|
Q
|
R
|
V
|
69 |
E
|
A
|
M
|
L
|
H
|
T
|
L
|
E
|
R
|
I
|
79 |
N
|
S
|
D
|
P
|
T
|
L
|
L
|
P
|
N
|
I
|
89 |
T
|
L
|
G
|
C
|
E
|
I
|
R
|
D
|
S
|
C
|
99 |
W
|
H
|
S
|
A
|
V
|
A
|
L
|
E
|
Q
|
S
|
109 |
I
|
E
|
F
|
I
|
R
|
D
|
S
|
L
|
I
|
S
|
119 |
S
|
E
|
E
|
E
|
E
|
G
|
L
|
V
|
R
|
C
|
129 |
V
|
D
|
G
|
S
|
S
|
S
|
S
|
F
|
R
|
S
|
139 |
K
|
K
|
P
|
I
|
V
|
G
|
V
|
I
|
G
|
P
|
149 |
G
|
S
|
S
|
S
|
V
|
A
|
I
|
Q
|
V
|
Q
|
159 |
N
|
L
|
L
|
Q
|
L
|
F
|
N
|
I
|
P
|
Q
|
169 |
I
|
A
|
Y
|
S
|
A
|
T
|
S
|
M
|
D
|
L
|
179 |
S
|
D
|
K
|
T
|
L
|
F
|
K
|
Y
|
F
|
M
|
189 |
R
|
V
|
V
|
P
|
S
|
D
|
A
|
Q
|
Q
|
A
|
199 |
R
|
A
|
M
|
V
|
D
|
I
|
V
|
K
|
R
|
Y
|
209 |
N
|
W
|
T
|
Y
|
V
|
S
|
A
|
V
|
H
|
T
|
219 |
E
|
G
|
N
|
Y
|
G
|
E
|
S
|
G
|
M
|
E
|
229 |
A
|
F
|
K
|
D
|
M
|
S
|
A
|
K
|
E
|
G
|
239 |
I
|
C
|
I
|
A
|
H
|
S
|
Y
|
K
|
I
|
Y
|
249 |
S
|
N
|
A
|
G
|
E
|
Q
|
S
|
F
|
D
|
K
|
259 |
L
|
L
|
K
|
K
|
L
|
T
|
S
|
H
|
L
|
P
|
269 |
K
|
A
|
R
|
V
|
V
|
A
|
C
|
F
|
C
|
E
|
279 |
G
|
M
|
T
|
V
|
R
|
G
|
L
|
L
|
M
|
A
|
289 |
M
|
R
|
R
|
L
|
G
|
L
|
A
|
G
|
E
|
F
|
299 |
L
|
L
|
L
|
G
|
S
|
D
|
G
|
W
|
A
|
D
|
309 |
R
|
Y
|
D
|
V
|
T
|
D
|
G
|
Y
|
Q
|
R
|
319 |
E
|
A
|
V
|
G
|
G
|
I
|
T
|
I
|
K
|
L
|
329 |
Q
|
S
|
P
|
D
|
V
|
K
|
W
|
F
|
D
|
D
|
339 |
Y
|
Y
|
L
|
K
|
L
|
R
|
P
|
E
|
T
|
N
|
349 |
H
|
R
|
N
|
P
|
W
|
F
|
Q
|
E
|
F
|
W
|
359 |
Q
|
H
|
R
|
F
|
Q
|
C
|
R
|
L
|
E
|
G
|
369 |
F
|
P
|
Q
|
E
|
N
|
S
|
K
|
Y
|
N
|
K
|
379 |
T
|
C
|
N
|
S
|
S
|
L
|
T
|
L
|
K
|
T
|
389 |
H
|
H
|
V
|
Q
|
D
|
S
|
K
|
M
|
G
|
F
|
399 |
V
|
I
|
N
|
A
|
I
|
Y
|
S
|
M
|
A
|
Y
|
409 |
G
|
L
|
H
|
N
|
M
|
Q
|
M
|
S
|
L
|
C
|
419 |
P
|
G
|
Y
|
A
|
G
|
L
|
C
|
D
|
A
|
M
|
429 |
K
|
P
|
I
|
D
|
G
|
R
|
K
|
L
|
L
|
E
|
439 |
S
|
L
|
M
|
K
|
T
|
N
|
F
|
T
|
G
|
V
|
449 |
S
|
G
|
D
|
T
|
I
|
L
|
F
|
D
|
E
|
N
|
459 |
G
|
D
|
S
|
P
|
G
|
R
|
Y
|
E
|
I
|
M
|
469 |
N
|
F
|
K
|
E
|
M
|
G
|
K
|
D
|
Y
|
F
|
479 |
D
|
Y
|
I
|
N
|
V
|
G
|
S
|
W
|
D
|
N
|
489 |
G
|
E
|
L
|
K
|
M
|
D
|
D
|
D
|
E
|
V
|
499 |
W
|
S
|
K
|
K
|
S
|
N
|
I
|
I
|
R
|
S
|
509 |
V
|
C
|
S
|
E
|
P
|
C
|
E
|
K
|
G
|
Q
|
519 |
I
|
K
|
V
|
I
|
R
|
K
|
G
|
E
|
V
|
S
|
529 |
C
|
C
|
W
|
T
|
C
|
T
|
P
|
C
|
K
|
E
|
539 |
N
|
E
|
Y
|
V
|
F
|
D
|
E
|
Y
|
T
|
C
|
549 |
K
|
A
|
C
|
Q
|
L
|
G
|
S
|
W
|
P
|
T
|
559 |
D
|
D
|
L
|
T
|
G
|
C
|
D
|
L
|
I
|
P
|
569 |
V
|
Q
|
Y
|
L
|
R
|
W
|
G
|
D
|
P
|
E
|
579 |
TM1 | |||||||||
P
|
I
|
A
|
A
|
V
|
V
|
F
|
A
|
C
|
L
|
589 |
G
|
L
|
L
|
A
|
T
|
L
|
F
|
V
|
T
|
V
|
599 |
ICL1 | |||||||||
V
|
F
|
I
|
I
|
Y
|
R
|
D
|
T
|
P
|
V
|
609 |
TM2 | |||||||||
V
|
K
|
S
|
S
|
S
|
R
|
E
|
L
|
C
|
Y
|
619 |
I
|
I
|
L
|
A
|
G
|
I
|
C
|
L
|
G
|
Y
|
629 |
L
|
C
|
T
|
F
|
C
|
L
|
I
|
A
|
K
|
P
|
639 |
TM3 | |||||||||
K
|
Q
|
I
|
Y
|
C
|
Y
|
L
|
Q
|
R
|
I
|
649 |
G
|
I
|
G
|
L
|
S
|
P
|
A
|
M
|
S
|
Y
|
659 |
S
|
A
|
L
|
V
|
T
|
K
|
T
|
N
|
R
|
I
|
669 |
ICL2 | |||||||||
A
|
R
|
I
|
L
|
A
|
G
|
S
|
K
|
K
|
K
|
679 |
I
|
C
|
T
|
K
|
K
|
P
|
R
|
F
|
M
|
S
|
689 |
TM4 | |||||||||
A
|
C
|
A
|
Q
|
L
|
V
|
I
|
A
|
F
|
I
|
699 |
L
|
I
|
C
|
I
|
Q
|
L
|
G
|
I
|
I
|
V
|
709 |
ECL2 | |||||||||
A
|
L
|
F
|
I
|
M
|
E
|
P
|
P
|
D
|
I
|
719 |
M
|
H
|
D
|
Y
|
P
|
S
|
I
|
R
|
E
|
V
|
729 |
TM5 | |||||||||
Y
|
L
|
I
|
C
|
N
|
T
|
T
|
N
|
L
|
G
|
739 |
|
|||||||||
V
|
V
|
A
|
P
|
L
|
G
|
Y
|
N
|
G
|
L
|
749 |
|
|||||||||
L
|
I
|
L
|
A
|
C
|
T
|
F
|
Y
|
A
|
F
|
759 |
ICL3 | |||||||||
K
|
T
|
R
|
N
|
V
|
P
|
A
|
N
|
F
|
N
|
769 |
TM6 | |||||||||
E
|
A
|
K
|
Y
|
I
|
A
|
F
|
T
|
M
|
Y
|
779 |
T
|
T
|
C
|
I
|
I
|
W
|
L
|
A
|
F
|
V
|
789 |
ECL3 | TM7 | |||||||||||
P
|
I
|
Y
|
F
|
G
|
S
|
N
|
Y
|
K
|
I
|
|||
799 |
I
|
T
|
M
|
C
|
F
|
S
|
V
|
S
|
L
|
S
|
809 |
A
|
T
|
V
|
A
|
L
|
G
|
C
|
M
|
F
|
V
|
819 |
P
|
K
|
V
|
Y
|
I
|
I
|
L
|
A
|
K
|
P
|
829 |
H8 | |||||||||
E
|
R
|
N
|
V
|
R
|
S
|
A
|
F
|
T
|
T
|
839 |
C-term | |||||||||
S
|
T
|
V
|
V
|
R
|
M
|
H
|
V
|
G
|
D
|
849 |
|
|||||||
G
|
K
|
S
|
S
|
S
|
A
|
A
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
742 | | T | A |
753 | | S | A |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|