lshr_human
NAME
7FIG
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
lshr_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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|
|
|
|
|
|
|
N-term | |||||||||
M
|
K
|
Q
|
R
|
F
|
S
|
A
|
L
|
Q
|
L
|
10 |
|
|
|
|
|
|
|
|
|
|
L
|
K
|
L
|
L
|
L
|
L
|
L
|
Q
|
P
|
P
|
20 |
|
|
|
|
|
|
|
|||
L
|
P
|
R
|
A
|
L
|
R
|
E
|
A
|
L
|
C
|
30 |
P
|
E
|
P
|
C
|
N
|
C
|
V
|
P
|
D
|
G
|
40 |
A
|
L
|
R
|
C
|
P
|
G
|
P
|
T
|
A
|
G
|
50 |
L
|
T
|
R
|
L
|
S
|
L
|
A
|
Y
|
L
|
P
|
60 |
V
|
K
|
V
|
I
|
P
|
S
|
Q
|
A
|
F
|
R
|
70 |
G
|
L
|
N
|
E
|
V
|
I
|
K
|
I
|
E
|
I
|
80 |
S
|
Q
|
I
|
D
|
S
|
L
|
E
|
R
|
I
|
E
|
90 |
A
|
N
|
A
|
F
|
D
|
N
|
L
|
L
|
N
|
L
|
100 |
S
|
E
|
I
|
L
|
I
|
Q
|
N
|
T
|
K
|
N
|
110 |
L
|
R
|
Y
|
I
|
E
|
P
|
G
|
A
|
F
|
I
|
120 |
N
|
L
|
P
|
R
|
L
|
K
|
Y
|
L
|
S
|
I
|
130 |
C
|
N
|
T
|
G
|
I
|
R
|
K
|
F
|
P
|
D
|
140 |
V
|
T
|
K
|
V
|
F
|
S
|
S
|
E
|
S
|
N
|
150 |
F
|
I
|
L
|
E
|
I
|
C
|
D
|
N
|
L
|
H
|
160 |
I
|
T
|
T
|
I
|
P
|
G
|
N
|
A
|
F
|
Q
|
170 |
G
|
M
|
N
|
N
|
E
|
S
|
V
|
T
|
L
|
K
|
180 |
L
|
Y
|
G
|
N
|
G
|
F
|
E
|
E
|
V
|
Q
|
190 |
S
|
H
|
A
|
F
|
N
|
G
|
T
|
T
|
L
|
T
|
200 |
S
|
L
|
E
|
L
|
K
|
E
|
N
|
V
|
H
|
L
|
210 |
E
|
K
|
M
|
H
|
N
|
G
|
A
|
F
|
R
|
G
|
220 |
A
|
T
|
G
|
P
|
K
|
T
|
L
|
D
|
I
|
S
|
230 |
S
|
T
|
K
|
L
|
Q
|
A
|
L
|
P
|
S
|
Y
|
240 |
G
|
L
|
E
|
S
|
I
|
Q
|
R
|
L
|
I
|
A
|
250 |
T
|
S
|
S
|
Y
|
S
|
L
|
K
|
K
|
L
|
P
|
260 |
S
|
R
|
E
|
T
|
F
|
V
|
N
|
L
|
L
|
E
|
270 |
A
|
T
|
L
|
T
|
Y
|
P
|
S
|
H
|
C
|
C
|
280 |
A
|
F
|
R
|
N
|
L
|
P
|
T
|
K
|
E
|
Q
|
290 |
N
|
F
|
S
|
H
|
S
|
I
|
S
|
E
|
N
|
F
|
300 |
S
|
K
|
Q
|
C
|
E
|
S
|
T
|
V
|
R
|
K
|
310 |
V
|
N
|
N
|
K
|
T
|
L
|
Y
|
S
|
S
|
M
|
320 |
L
|
A
|
E
|
S
|
E
|
L
|
S
|
G
|
W
|
D
|
330 |
Y
|
E
|
Y
|
G
|
F
|
C
|
L
|
P
|
K
|
T
|
340 |
P
|
R
|
C
|
A
|
P
|
E
|
P
|
D
|
A
|
F
|
350 |
N
|
P
|
C
|
E
|
D
|
I
|
M
|
G
|
Y
|
D
|
360 |
TM1 | |||||||||
F
|
L
|
R
|
V
|
L
|
I
|
W
|
L
|
I
|
N
|
370 |
I
|
L
|
A
|
I
|
M
|
G
|
N
|
M
|
T
|
V
|
380 |
L
|
F
|
V
|
L
|
L
|
T
|
S
|
R
|
Y
|
K
|
390 |
ICL1 | TM2 | ||||||||
L
|
T
|
V
|
P
|
R
|
F
|
L
|
M
|
C
|
N
|
400 |
L
|
S
|
F
|
A
|
D
|
F
|
C
|
M
|
G
|
L
|
410 |
Y
|
L
|
L
|
L
|
I
|
A
|
S
|
V
|
D
|
S
|
420 |
ECL1 | |||||||||
Q
|
T
|
K
|
G
|
Q
|
Y
|
Y
|
N
|
H
|
A
|
430 |
TM3 | |||||||||
I
|
D
|
W
|
Q
|
T
|
G
|
S
|
G
|
C
|
S
|
440 |
T
|
A
|
G
|
F
|
F
|
T
|
V
|
F
|
A
|
S
|
450 |
E
|
L
|
S
|
V
|
Y
|
T
|
L
|
T
|
V
|
I
|
460 |
T
|
L
|
E
|
R
|
W
|
H
|
T
|
I
|
T
|
Y
|
470 |
ICL2 | |||||||||
A
|
I
|
H
|
L
|
D
|
Q
|
K
|
L
|
R
|
L
|
480 |
TM4 | |||||||||
R
|
H
|
A
|
I
|
L
|
I
|
M
|
L
|
G
|
G
|
490 |
W
|
L
|
F
|
S
|
S
|
L
|
I
|
A
|
M
|
L
|
500 |
ECL2 | |||||||||
P
|
L
|
V
|
G
|
V
|
S
|
N
|
Y
|
M
|
K
|
510 |
V
|
S
|
I
|
C
|
F
|
P
|
M
|
D
|
V
|
E
|
520 |
TM5 | |||||||||
T
|
T
|
L
|
S
|
Q
|
V
|
Y
|
I
|
L
|
T
|
530 |
I
|
L
|
I
|
L
|
N
|
V
|
V
|
A
|
F
|
F
|
540 |
I
|
I
|
C
|
A
|
C
|
Y
|
I
|
K
|
I
|
Y
|
550 |
ICL3 | |||||||||
F
|
A
|
V
|
R
|
N
|
P
|
E
|
L
|
M
|
A
|
560 |
TM6 | |||||||||
T
|
N
|
K
|
D
|
T
|
K
|
I
|
A
|
K
|
K
|
570 |
M
|
A
|
I
|
L
|
I
|
F
|
T
|
D
|
F
|
T
|
580 |
C
|
M
|
A
|
P
|
I
|
S
|
F
|
F
|
A
|
I
|
590 |
ECL3 | |||||||||
S
|
A
|
A
|
F
|
K
|
V
|
P
|
L
|
I
|
T
|
600 |
TM7 | |||||||||
V
|
T
|
N
|
S
|
K
|
V
|
L
|
L
|
V
|
L
|
610 |
F
|
Y
|
P
|
I
|
N
|
S
|
C
|
A
|
N
|
P
|
620 |
H8 | |||||||||
F
|
L
|
Y
|
A
|
I
|
F
|
T
|
K
|
T
|
F
|
630 |
Q
|
R
|
D
|
F
|
F
|
L
|
L
|
L
|
S
|
K
|
640 |
C-term | |||||||||
F
|
G
|
C
|
C
|
K
|
R
|
R
|
A
|
E
|
L
|
650 |
Y
|
R
|
R
|
K
|
D
|
F
|
S
|
A
|
Y
|
T
|
660 |
S
|
N
|
C
|
K
|
N
|
G
|
F
|
T
|
G
|
S
|
670 |
N
|
K
|
P
|
S
|
Q
|
S
|
T
|
L
|
K
|
L
|
680 |
S
|
T
|
L
|
H
|
C
|
Q
|
G
|
T
|
A
|
L
|
690 |
L
|
D
|
K
|
T
|
R
|
Y
|
T
|
E
|
C
|
|
|||||||||
N-term | |||||||||
|
A
|
L
|
C
|
P
|
E
|
P
|
C
|
N
|
C
|
36 |
V
|
P
|
D
|
G
|
A
|
L
|
R
|
C
|
P
|
G
|
46 |
P
|
T
|
A
|
G
|
L
|
T
|
R
|
L
|
S
|
L
|
56 |
A
|
Y
|
L
|
P
|
V
|
K
|
V
|
I
|
P
|
S
|
66 |
Q
|
A
|
F
|
R
|
G
|
L
|
N
|
E
|
V
|
I
|
76 |
K
|
I
|
E
|
I
|
S
|
Q
|
I
|
D
|
S
|
L
|
86 |
E
|
R
|
I
|
E
|
A
|
N
|
A
|
F
|
D
|
N
|
96 |
L
|
L
|
N
|
L
|
S
|
E
|
I
|
L
|
I
|
Q
|
106 |
N
|
T
|
K
|
N
|
L
|
R
|
Y
|
I
|
E
|
P
|
116 |
G
|
A
|
F
|
I
|
N
|
L
|
P
|
R
|
L
|
K
|
126 |
Y
|
L
|
S
|
I
|
C
|
N
|
T
|
G
|
I
|
R
|
136 |
K
|
F
|
P
|
D
|
V
|
T
|
K
|
V
|
F
|
S
|
146 |
S
|
E
|
S
|
N
|
F
|
I
|
L
|
E
|
I
|
C
|
156 |
D
|
N
|
L
|
H
|
I
|
T
|
T
|
I
|
P
|
G
|
166 |
N
|
A
|
F
|
Q
|
G
|
M
|
N
|
N
|
E
|
S
|
176 |
V
|
T
|
L
|
K
|
L
|
Y
|
G
|
N
|
G
|
F
|
186 |
E
|
E
|
V
|
Q
|
S
|
H
|
A
|
F
|
N
|
G
|
196 |
T
|
T
|
L
|
T
|
S
|
L
|
E
|
L
|
K
|
E
|
206 |
N
|
V
|
H
|
L
|
E
|
K
|
M
|
H
|
N
|
G
|
216 |
A
|
F
|
R
|
G
|
A
|
T
|
G
|
P
|
K
|
T
|
226 |
L
|
D
|
I
|
S
|
S
|
T
|
K
|
L
|
Q
|
A
|
236 |
L
|
P
|
S
|
Y
|
G
|
L
|
E
|
S
|
I
|
Q
|
246 |
R
|
L
|
I
|
A
|
T
|
S
|
S
|
Y
|
S
|
L
|
256 |
K
|
K
|
L
|
P
|
S
|
R
|
E
|
T
|
F
|
V
|
266 |
N
|
L
|
L
|
E
|
A
|
T
|
L
|
T
|
Y
|
P
|
276 |
S
|
H
|
C
|
C
|
A
|
F
|
R
|
N
|
L
|
P
|
286 |
T
|
K
|
E
|
Q
|
N
|
F
|
S
|
H
|
S
|
I
|
296 |
S
|
E
|
N
|
F
|
S
|
K
|
Q
|
C
|
E
|
S
|
306 |
T
|
V
|
R
|
K
|
V
|
N
|
N
|
K
|
T
|
L
|
316 |
Y
|
S
|
S
|
M
|
L
|
A
|
E
|
S
|
E
|
L
|
326 |
S
|
G
|
W
|
D
|
Y
|
E
|
Y
|
G
|
F
|
C
|
336 |
L
|
P
|
K
|
T
|
P
|
R
|
C
|
A
|
P
|
E
|
346 |
P
|
D
|
A
|
F
|
N
|
P
|
C
|
E
|
D
|
I
|
356 |
TM1 | |||||||||
M
|
G
|
Y
|
D
|
F
|
L
|
R
|
V
|
L
|
I
|
366 |
W
|
L
|
I
|
N
|
I
|
L
|
A
|
I
|
M
|
G
|
376 |
N
|
M
|
T
|
V
|
L
|
F
|
V
|
L
|
L
|
T
|
386 |
ICL1 | TM2 | ||||||||
S
|
R
|
Y
|
K
|
L
|
T
|
V
|
P
|
R
|
F
|
396 |
L
|
M
|
C
|
N
|
L
|
S
|
F
|
A
|
D
|
F
|
406 |
C
|
M
|
G
|
L
|
Y
|
L
|
L
|
L
|
I
|
A
|
416 |
ECL1 | |||||||||
S
|
V
|
D
|
S
|
Q
|
T
|
K
|
G
|
Q
|
Y
|
426 |
Y
|
N
|
H
|
A
|
I
|
D
|
W
|
Q
|
T
|
G
|
436 |
TM3 | |||||||||
S
|
G
|
C
|
S
|
T
|
A
|
G
|
F
|
F
|
T
|
446 |
V
|
F
|
A
|
S
|
E
|
L
|
S
|
V
|
Y
|
T
|
456 |
L
|
T
|
V
|
I
|
T
|
L
|
E
|
R
|
W
|
H
|
466 |
ICL2 | |||||||||
T
|
I
|
T
|
Y
|
A
|
I
|
H
|
L
|
D
|
Q
|
476 |
TM4 | |||||||||
K
|
L
|
R
|
L
|
R
|
H
|
A
|
I
|
L
|
I
|
486 |
M
|
L
|
G
|
G
|
W
|
L
|
F
|
S
|
S
|
L
|
496 |
I
|
A
|
M
|
L
|
P
|
L
|
V
|
G
|
V
|
S
|
506 |
ECL2 | |||||||||
N
|
Y
|
M
|
K
|
V
|
S
|
I
|
C
|
F
|
P
|
516 |
TM5 | |||||||||
M
|
D
|
V
|
E
|
T
|
T
|
L
|
S
|
Q
|
V
|
526 |
Y
|
I
|
L
|
T
|
I
|
L
|
I
|
L
|
N
|
V
|
536 |
V
|
A
|
F
|
F
|
I
|
I
|
C
|
A
|
C
|
Y
|
546 |
I
|
K
|
I
|
Y
|
F
|
A
|
V
|
R
|
N
|
P
|
556 |
ICL3 | TM6 | ||||||||
E
|
L
|
M
|
A
|
T
|
N
|
K
|
D
|
T
|
K
|
566 |
I
|
A
|
K
|
K
|
M
|
A
|
I
|
L
|
I
|
F
|
576 |
T
|
D
|
F
|
T
|
C
|
M
|
A
|
P
|
I
|
S
|
586 |
F
|
F
|
A
|
I
|
S
|
A
|
A
|
F
|
K
|
V
|
596 |
ECL3 | TM7 | ||||||||
P
|
L
|
I
|
T
|
V
|
T
|
N
|
S
|
K
|
V
|
606 |
L
|
L
|
V
|
L
|
F
|
Y
|
P
|
I
|
N
|
S
|
616 |
C
|
A
|
N
|
P
|
F
|
L
|
Y
|
A
|
I
|
F
|
626 |
H8 | |||||||||
T
|
K
|
T
|
F
|
Q
|
R
|
D
|
F
|
F
|
L
|
636 |
L
|
L
|
S
|
K
|
F
|
G
|
C
|
C
|
K
|
R
|
646 |
C-term | |||||||||
R
|
A
|
E
|
L
|
Y
|
R
|
R
|
K
|
D
|
F
|
656 |
S
|
A
|
Y
|
T
|
S
|
N
|
C
|
K
|
N
|
G
|
666 |
F
|
T
|
G
|
S
|
N
|
K
|
P
|
S
|
Q
|
S
|
676 |
T
|
L
|
K
|
L
|
S
|
T
|
L
|
H
|
C
|
Q
|
686 |
G
|
T
|
A
|
L
|
L
|
D
|
K
|
T
|
R
|
Y
|
696 |
T
|
E
|
C
|
699 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|