acm2_human
NAME
7T94
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
acm2_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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N
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N
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S
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T
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N
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S
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S
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N
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N
|
10 |
S
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L
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A
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L
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T
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S
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P
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Y
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K
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T
|
20 |
TM1 | |||||||||
F
|
E
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V
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V
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F
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I
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V
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L
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V
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A
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30 |
G
|
S
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L
|
S
|
L
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V
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T
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I
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I
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G
|
40 |
N
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I
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L
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V
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M
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V
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S
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I
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K
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V
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50 |
ICL1 | TM2 | ||||||||
N
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R
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H
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L
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Q
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T
|
V
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N
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N
|
Y
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60 |
F
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L
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F
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S
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L
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A
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C
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A
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D
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L
|
70 |
I
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I
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G
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V
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F
|
S
|
M
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N
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L
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Y
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80 |
T
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L
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Y
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T
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V
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I
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G
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Y
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W
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P
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90 |
ECL1 | TM3 | ||||||||
L
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G
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P
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V
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V
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C
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D
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L
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W
|
L
|
100 |
A
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L
|
D
|
Y
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V
|
V
|
S
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N
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A
|
S
|
110 |
V
|
M
|
N
|
L
|
L
|
I
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I
|
S
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F
|
D
|
120 |
R
|
Y
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F
|
C
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V
|
T
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K
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P
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L
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T
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130 |
ICL2 | TM4 | ||||||||
Y
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P
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V
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K
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R
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T
|
T
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K
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M
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A
|
140 |
G
|
M
|
M
|
I
|
A
|
A
|
A
|
W
|
V
|
L
|
150 |
S
|
F
|
I
|
L
|
W
|
A
|
P
|
A
|
I
|
L
|
160 |
F
|
W
|
Q
|
F
|
I
|
V
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G
|
V
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R
|
T
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170 |
ECL2 | |||||||||
V
|
E
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D
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G
|
E
|
C
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Y
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I
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Q
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F
|
180 |
TM5 | |||||||||
F
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S
|
N
|
A
|
A
|
V
|
T
|
F
|
G
|
T
|
190 |
A
|
I
|
A
|
A
|
F
|
Y
|
L
|
P
|
V
|
I
|
200 |
I
|
M
|
T
|
V
|
L
|
Y
|
W
|
H
|
I
|
S
|
210 |
R
|
A
|
S
|
K
|
S
|
R
|
I
|
K
|
K
|
D
|
220 |
ICL3 | |||||||||
K
|
K
|
E
|
P
|
V
|
A
|
N
|
Q
|
D
|
P
|
230 |
|
|
|
|
|
|
|
|
||
V
|
S
|
P
|
S
|
L
|
V
|
Q
|
G
|
R
|
I
|
240 |
|
|
|
|
|
|
|
|
|
|
V
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K
|
P
|
N
|
N
|
N
|
N
|
M
|
P
|
S
|
250 |
|
|
|
|
|
|
|
|
|
|
S
|
D
|
D
|
G
|
L
|
E
|
H
|
N
|
K
|
I
|
260 |
|
|
|
|
|
|
|
|
|
|
Q
|
N
|
G
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K
|
A
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P
|
R
|
D
|
P
|
V
|
270 |
|
|
|
|
|
|
|
|
|
|
T
|
E
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N
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C
|
V
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Q
|
G
|
E
|
E
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K
|
280 |
|
|
|
|
|
|
|
|
|
|
E
|
S
|
S
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N
|
D
|
S
|
T
|
S
|
V
|
S
|
290 |
|
|
|
|
|
|
|
|
|
|
A
|
V
|
A
|
S
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N
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M
|
R
|
D
|
D
|
E
|
300 |
|
|
|
|
|
|
|
|
|
|
I
|
T
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Q
|
D
|
E
|
N
|
T
|
V
|
S
|
T
|
310 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
G
|
H
|
S
|
K
|
D
|
E
|
N
|
S
|
320 |
|
|
|
|
|
|
|
|
|
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K
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Q
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T
|
C
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I
|
R
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I
|
G
|
T
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K
|
330 |
|
|
|
|
|
|
|
|
|
|
T
|
P
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K
|
S
|
D
|
S
|
C
|
T
|
P
|
T
|
340 |
|
|
|
|
|
|
|
|
|
|
N
|
T
|
T
|
V
|
E
|
V
|
V
|
G
|
S
|
S
|
350 |
|
|
|
|
|
|
|
|
|
|
G
|
Q
|
N
|
G
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D
|
E
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K
|
Q
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N
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I
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360 |
|
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|
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|||
V
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A
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R
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K
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I
|
V
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K
|
M
|
T
|
K
|
370 |
TM6 | |||||||||
Q
|
P
|
A
|
K
|
K
|
K
|
P
|
P
|
P
|
S
|
380 |
R
|
E
|
K
|
K
|
V
|
T
|
R
|
T
|
I
|
L
|
390 |
A
|
I
|
L
|
L
|
A
|
F
|
I
|
I
|
T
|
W
|
400 |
A
|
P
|
Y
|
N
|
V
|
M
|
V
|
L
|
I
|
N
|
410 |
ECL3 | TM7 | ||||||||
T
|
F
|
C
|
A
|
P
|
C
|
I
|
P
|
N
|
T
|
420 |
V
|
W
|
T
|
I
|
G
|
Y
|
W
|
L
|
C
|
Y
|
430 |
I
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N
|
S
|
T
|
I
|
N
|
P
|
A
|
C
|
Y
|
440 |
H8 | |||||||||
A
|
L
|
C
|
N
|
A
|
T
|
F
|
K
|
K
|
T
|
450 |
F
|
K
|
H
|
L
|
L
|
M
|
C
|
H
|
Y
|
K
|
460 |
C-term | |||||
N
|
I
|
G
|
A
|
T
|
R
|
|
|
|
|||||||
N-term | |||||||||
|
S
|
T
|
D
|
S
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S
|
D
|
N
|
S
|
L
|
12 |
TM1 | |||||||||
A
|
L
|
T
|
S
|
P
|
Y
|
K
|
T
|
F
|
E
|
22 |
V
|
V
|
F
|
I
|
V
|
L
|
V
|
A
|
G
|
S
|
32 |
L
|
S
|
L
|
V
|
T
|
I
|
I
|
G
|
N
|
I
|
42 |
L
|
V
|
M
|
V
|
S
|
I
|
K
|
V
|
N
|
R
|
52 |
ICL1 | TM2 | ||||||||
H
|
L
|
Q
|
T
|
V
|
N
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N
|
Y
|
F
|
L
|
62 |
F
|
S
|
L
|
A
|
C
|
A
|
D
|
L
|
I
|
I
|
72 |
G
|
V
|
F
|
S
|
M
|
N
|
L
|
Y
|
T
|
L
|
82 |
ECL1 | |||||||||
Y
|
T
|
V
|
I
|
G
|
Y
|
W
|
P
|
L
|
G
|
92 |
TM3 | |||||||||
P
|
V
|
V
|
C
|
D
|
L
|
W
|
L
|
A
|
L
|
102 |
D
|
Y
|
V
|
V
|
S
|
N
|
A
|
S
|
V
|
M
|
112 |
N
|
L
|
L
|
I
|
I
|
S
|
F
|
D
|
R
|
Y
|
122 |
ICL2 | |||||||||
F
|
C
|
V
|
T
|
K
|
P
|
L
|
T
|
Y
|
P
|
132 |
TM4 | |||||||||
V
|
K
|
R
|
T
|
T
|
K
|
M
|
A
|
G
|
M
|
142 |
M
|
I
|
A
|
A
|
A
|
W
|
V
|
L
|
S
|
F
|
152 |
I
|
L
|
W
|
A
|
P
|
A
|
I
|
L
|
F
|
W
|
162 |
ECL2 | |||||||||
Q
|
F
|
I
|
V
|
G
|
V
|
R
|
T
|
V
|
E
|
172 |
D
|
G
|
E
|
C
|
Y
|
I
|
Q
|
F
|
F
|
S
|
182 |
TM5 | |||||||||
N
|
A
|
A
|
V
|
T
|
F
|
G
|
T
|
A
|
I
|
192 |
A
|
A
|
F
|
Y
|
L
|
P
|
V
|
I
|
I
|
M
|
202 |
T
|
V
|
L
|
Y
|
W
|
H
|
I
|
S
|
R
|
A
|
212 |
S
|
K
|
S
|
R
|
I
|
K
|
K
|
D
|
K
|
K
|
222 |
|
|||||||||||||||||||||||||||||||||||||||
ICL3 | |||||||||||||||||||||||||||||||||||||||
E
|
P
|
V
|
A
|
N
|
Q
|
D
|
P
|
V
|
S
|
|
M
|
T
|
K
|
Q
|
P
|
A
|
K
|
K
|
K
|
||||||||||||||||||||
232 | 376 |
TM6 | |||||||||
P
|
P
|
P
|
S
|
R
|
E
|
K
|
K
|
V
|
T
|
386 |
R
|
T
|
I
|
L
|
A
|
I
|
L
|
L
|
A
|
F
|
396 |
I
|
I
|
T
|
W
|
A
|
P
|
Y
|
N
|
V
|
M
|
406 |
V
|
L
|
I
|
N
|
T
|
F
|
C
|
A
|
P
|
C
|
416 |
ECL3 | TM7 | ||||||||
I
|
P
|
N
|
T
|
V
|
W
|
T
|
I
|
G
|
Y
|
426 |
W
|
L
|
C
|
Y
|
I
|
N
|
S
|
T
|
I
|
N
|
436 |
H8 | |||||||||
P
|
A
|
C
|
Y
|
A
|
L
|
C
|
N
|
A
|
T
|
446 |
F
|
K
|
K
|
T
|
F
|
K
|
H
|
L
|
L
|
M
|
456 |
C-term | |||||||||
C
|
H
|
Y
|
K
|
N
|
I
|
G
|
A
|
T
|
R
|
466 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
6 | | N | D |
9 | | N | D |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|