tshr_human
NAME
7XW7
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
tshr_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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|
|
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|
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N-term | |||||||||
M
|
R
|
P
|
A
|
D
|
L
|
L
|
Q
|
L
|
V
|
10 |
|
|
|
|
|
|
|
|
|
|
L
|
L
|
L
|
D
|
L
|
P
|
R
|
D
|
L
|
G
|
20 |
G
|
M
|
G
|
C
|
S
|
S
|
P
|
P
|
C
|
E
|
30 |
C
|
H
|
Q
|
E
|
E
|
D
|
F
|
R
|
V
|
T
|
40 |
C
|
K
|
D
|
I
|
Q
|
R
|
I
|
P
|
S
|
L
|
50 |
P
|
P
|
S
|
T
|
Q
|
T
|
L
|
K
|
L
|
I
|
60 |
E
|
T
|
H
|
L
|
R
|
T
|
I
|
P
|
S
|
H
|
70 |
A
|
F
|
S
|
N
|
L
|
P
|
N
|
I
|
S
|
R
|
80 |
I
|
Y
|
V
|
S
|
I
|
D
|
V
|
T
|
L
|
Q
|
90 |
Q
|
L
|
E
|
S
|
H
|
S
|
F
|
Y
|
N
|
L
|
100 |
S
|
K
|
V
|
T
|
H
|
I
|
E
|
I
|
R
|
N
|
110 |
T
|
R
|
N
|
L
|
T
|
Y
|
I
|
D
|
P
|
D
|
120 |
A
|
L
|
K
|
E
|
L
|
P
|
L
|
L
|
K
|
F
|
130 |
L
|
G
|
I
|
F
|
N
|
T
|
G
|
L
|
K
|
M
|
140 |
F
|
P
|
D
|
L
|
T
|
K
|
V
|
Y
|
S
|
T
|
150 |
D
|
I
|
F
|
F
|
I
|
L
|
E
|
I
|
T
|
D
|
160 |
N
|
P
|
Y
|
M
|
T
|
S
|
I
|
P
|
V
|
N
|
170 |
A
|
F
|
Q
|
G
|
L
|
C
|
N
|
E
|
T
|
L
|
180 |
T
|
L
|
K
|
L
|
Y
|
N
|
N
|
G
|
F
|
T
|
190 |
S
|
V
|
Q
|
G
|
Y
|
A
|
F
|
N
|
G
|
T
|
200 |
K
|
L
|
D
|
A
|
V
|
Y
|
L
|
N
|
K
|
N
|
210 |
K
|
Y
|
L
|
T
|
V
|
I
|
D
|
K
|
D
|
A
|
220 |
F
|
G
|
G
|
V
|
Y
|
S
|
G
|
P
|
S
|
L
|
230 |
L
|
D
|
V
|
S
|
Q
|
T
|
S
|
V
|
T
|
A
|
240 |
L
|
P
|
S
|
K
|
G
|
L
|
E
|
H
|
L
|
K
|
250 |
E
|
L
|
I
|
A
|
R
|
N
|
T
|
W
|
T
|
L
|
260 |
K
|
K
|
L
|
P
|
L
|
S
|
L
|
S
|
F
|
L
|
270 |
H
|
L
|
T
|
R
|
A
|
D
|
L
|
S
|
Y
|
P
|
280 |
S
|
H
|
C
|
C
|
A
|
F
|
K
|
N
|
Q
|
K
|
290 |
K
|
I
|
R
|
G
|
I
|
L
|
E
|
S
|
L
|
M
|
300 |
C
|
N
|
E
|
S
|
S
|
M
|
Q
|
S
|
L
|
R
|
310 |
|
|
|
|
||||||
Q
|
R
|
K
|
S
|
V
|
N
|
A
|
L
|
N
|
S
|
320 |
|
|
|
|
|
|
|
|
|
|
P
|
L
|
H
|
Q
|
E
|
Y
|
E
|
E
|
N
|
L
|
330 |
|
|
|
|
|
|
|
|
|
|
G
|
D
|
S
|
I
|
V
|
G
|
Y
|
K
|
E
|
K
|
340 |
|
|
|
|
|
|
|
|
|
|
S
|
K
|
F
|
Q
|
D
|
T
|
H
|
N
|
N
|
A
|
350 |
|
|
|
|
|
|
|
|
|
|
H
|
Y
|
Y
|
V
|
F
|
F
|
E
|
E
|
Q
|
E
|
360 |
|
|
|
|
|
|
||||
D
|
E
|
I
|
I
|
G
|
F
|
G
|
Q
|
E
|
L
|
370 |
K
|
N
|
P
|
Q
|
E
|
E
|
T
|
L
|
Q
|
A
|
380 |
F
|
D
|
S
|
H
|
Y
|
D
|
Y
|
T
|
I
|
C
|
390 |
G
|
D
|
S
|
E
|
D
|
M
|
V
|
C
|
T
|
P
|
400 |
K
|
S
|
D
|
E
|
F
|
N
|
P
|
C
|
E
|
D
|
410 |
TM1 | |||||||||
I
|
M
|
G
|
Y
|
K
|
F
|
L
|
R
|
I
|
V
|
420 |
V
|
W
|
F
|
V
|
S
|
L
|
L
|
A
|
L
|
L
|
430 |
G
|
N
|
V
|
F
|
V
|
L
|
L
|
I
|
L
|
L
|
440 |
ICL1 | TM2 | ||||||||
T
|
S
|
H
|
Y
|
K
|
L
|
N
|
V
|
P
|
R
|
450 |
F
|
L
|
M
|
C
|
N
|
L
|
A
|
F
|
A
|
D
|
460 |
F
|
C
|
M
|
G
|
M
|
Y
|
L
|
L
|
L
|
I
|
470 |
A
|
S
|
V
|
D
|
L
|
Y
|
T
|
H
|
S
|
E
|
480 |
ECL1 | |||||||||
Y
|
Y
|
N
|
H
|
A
|
I
|
D
|
W
|
Q
|
T
|
490 |
TM3 | |||||||||
G
|
P
|
G
|
C
|
N
|
T
|
A
|
G
|
F
|
F
|
500 |
T
|
V
|
F
|
A
|
S
|
E
|
L
|
S
|
V
|
Y
|
510 |
T
|
L
|
T
|
V
|
I
|
T
|
L
|
E
|
R
|
W
|
520 |
ICL2 | |||||||||
Y
|
A
|
I
|
T
|
F
|
A
|
M
|
R
|
L
|
D
|
530 |
TM4 | |||||||||
R
|
K
|
I
|
R
|
L
|
R
|
H
|
A
|
C
|
A
|
540 |
I
|
M
|
V
|
G
|
G
|
W
|
V
|
C
|
C
|
F
|
550 |
L
|
L
|
A
|
L
|
L
|
P
|
L
|
V
|
G
|
I
|
560 |
ECL2 | |||||||||
S
|
S
|
Y
|
A
|
K
|
V
|
S
|
I
|
C
|
L
|
570 |
TM5 | |||||||||
P
|
M
|
D
|
T
|
E
|
T
|
P
|
L
|
A
|
L
|
580 |
A
|
Y
|
I
|
V
|
F
|
V
|
L
|
T
|
L
|
N
|
590 |
I
|
V
|
A
|
F
|
V
|
I
|
V
|
C
|
C
|
C
|
600 |
Y
|
V
|
K
|
I
|
Y
|
I
|
T
|
V
|
R
|
N
|
610 |
ICL3 | TM6 | ||||||||
P
|
Q
|
Y
|
N
|
P
|
G
|
D
|
K
|
D
|
T
|
620 |
K
|
I
|
A
|
K
|
R
|
M
|
A
|
V
|
L
|
I
|
630 |
F
|
T
|
D
|
F
|
I
|
C
|
M
|
A
|
P
|
I
|
640 |
S
|
F
|
Y
|
A
|
L
|
S
|
A
|
I
|
L
|
N
|
650 |
ECL3 | TM7 | ||||||||
K
|
P
|
L
|
I
|
T
|
V
|
S
|
N
|
S
|
K
|
660 |
I
|
L
|
L
|
V
|
L
|
F
|
Y
|
P
|
L
|
N
|
670 |
S
|
C
|
A
|
N
|
P
|
F
|
L
|
Y
|
A
|
I
|
680 |
H8 | |||||||||
F
|
T
|
K
|
A
|
F
|
Q
|
R
|
D
|
V
|
F
|
690 |
I
|
L
|
L
|
S
|
K
|
F
|
G
|
I
|
C
|
K
|
700 |
C-term | |||||||||
R
|
Q
|
A
|
Q
|
A
|
Y
|
R
|
G
|
Q
|
R
|
710 |
V
|
P
|
P
|
K
|
N
|
S
|
T
|
D
|
I
|
Q
|
720 |
V
|
Q
|
K
|
V
|
T
|
H
|
D
|
M
|
R
|
Q
|
730 |
G
|
L
|
H
|
N
|
M
|
E
|
D
|
V
|
Y
|
E
|
740 |
L
|
I
|
E
|
N
|
S
|
H
|
L
|
T
|
P
|
K
|
750 |
K
|
Q
|
G
|
Q
|
I
|
S
|
E
|
E
|
Y
|
M
|
760 |
Q
|
T
|
V
|
L
|
|
|||||||||
N-term | |||||||||
|
G
|
M
|
G
|
C
|
S
|
S
|
P
|
P
|
C
|
29 |
E
|
C
|
H
|
Q
|
E
|
E
|
D
|
F
|
R
|
V
|
39 |
T
|
C
|
K
|
D
|
I
|
Q
|
R
|
I
|
P
|
S
|
49 |
L
|
P
|
P
|
S
|
T
|
Q
|
T
|
L
|
K
|
L
|
59 |
I
|
E
|
T
|
H
|
L
|
R
|
T
|
I
|
P
|
S
|
69 |
H
|
A
|
F
|
S
|
N
|
L
|
P
|
N
|
I
|
S
|
79 |
R
|
I
|
Y
|
V
|
S
|
I
|
D
|
V
|
T
|
L
|
89 |
Q
|
Q
|
L
|
E
|
S
|
H
|
S
|
F
|
Y
|
N
|
99 |
L
|
S
|
K
|
V
|
T
|
H
|
I
|
E
|
I
|
R
|
109 |
N
|
T
|
R
|
N
|
L
|
T
|
Y
|
I
|
D
|
P
|
119 |
D
|
A
|
L
|
K
|
E
|
L
|
P
|
L
|
L
|
K
|
129 |
F
|
L
|
G
|
I
|
F
|
N
|
T
|
G
|
L
|
K
|
139 |
M
|
F
|
P
|
D
|
L
|
T
|
K
|
V
|
Y
|
S
|
149 |
T
|
D
|
I
|
F
|
F
|
I
|
L
|
E
|
I
|
T
|
159 |
D
|
N
|
P
|
Y
|
M
|
T
|
S
|
I
|
P
|
V
|
169 |
N
|
A
|
F
|
Q
|
G
|
L
|
C
|
N
|
E
|
T
|
179 |
L
|
T
|
L
|
K
|
L
|
Y
|
N
|
N
|
G
|
F
|
189 |
T
|
S
|
V
|
Q
|
G
|
Y
|
A
|
F
|
N
|
G
|
199 |
T
|
K
|
L
|
D
|
A
|
V
|
Y
|
L
|
N
|
K
|
209 |
N
|
K
|
Y
|
L
|
T
|
V
|
I
|
D
|
K
|
D
|
219 |
A
|
F
|
G
|
G
|
V
|
Y
|
S
|
G
|
P
|
S
|
229 |
L
|
L
|
D
|
V
|
S
|
Q
|
T
|
S
|
V
|
T
|
239 |
A
|
L
|
P
|
S
|
K
|
G
|
L
|
E
|
H
|
L
|
249 |
K
|
E
|
L
|
I
|
A
|
R
|
N
|
T
|
W
|
T
|
259 |
L
|
K
|
K
|
L
|
P
|
L
|
S
|
L
|
S
|
F
|
269 |
L
|
H
|
L
|
T
|
R
|
A
|
D
|
L
|
S
|
Y
|
279 |
P
|
S
|
H
|
C
|
C
|
A
|
F
|
K
|
N
|
Q
|
289 |
K
|
K
|
I
|
R
|
G
|
I
|
L
|
E
|
S
|
L
|
299 |
M
|
C
|
N
|
E
|
S
|
S
|
M
|
Q
|
S
|
L
|
309 |
|
|||||||||
R
|
Q
|
R
|
K
|
S
|
V
|
N
|
|
G
|
Q
|
368 |
E
|
L
|
K
|
N
|
P
|
Q
|
E
|
E
|
T
|
L
|
378 |
Q
|
A
|
F
|
D
|
S
|
H
|
Y
|
D
|
Y
|
T
|
388 |
I
|
C
|
G
|
D
|
S
|
E
|
D
|
M
|
V
|
C
|
398 |
T
|
P
|
K
|
S
|
D
|
E
|
F
|
N
|
P
|
C
|
408 |
TM1 | |||||||||
E
|
D
|
I
|
M
|
G
|
Y
|
K
|
F
|
L
|
R
|
418 |
I
|
V
|
V
|
W
|
F
|
V
|
S
|
L
|
L
|
A
|
428 |
L
|
L
|
G
|
N
|
V
|
F
|
V
|
L
|
L
|
I
|
438 |
ICL1 | |||||||||
L
|
L
|
T
|
S
|
H
|
Y
|
K
|
L
|
N
|
V
|
448 |
TM2 | |||||||||
P
|
R
|
F
|
L
|
M
|
C
|
N
|
L
|
A
|
F
|
458 |
A
|
D
|
F
|
C
|
M
|
G
|
M
|
Y
|
L
|
L
|
468 |
L
|
I
|
A
|
S
|
V
|
D
|
L
|
Y
|
T
|
H
|
478 |
ECL1 | |||||||||
S
|
E
|
Y
|
Y
|
N
|
H
|
A
|
I
|
D
|
W
|
488 |
TM3 | |||||||||
Q
|
T
|
G
|
P
|
G
|
C
|
N
|
T
|
A
|
G
|
498 |
F
|
F
|
T
|
V
|
F
|
A
|
S
|
E
|
L
|
S
|
508 |
V
|
Y
|
T
|
L
|
T
|
V
|
I
|
T
|
L
|
E
|
518 |
R
|
W
|
Y
|
A
|
I
|
T
|
F
|
A
|
M
|
R
|
528 |
ICL2 | TM4 | ||||||||
L
|
D
|
R
|
K
|
I
|
R
|
L
|
R
|
H
|
A
|
538 |
C
|
A
|
I
|
M
|
V
|
G
|
G
|
W
|
V
|
C
|
548 |
C
|
F
|
L
|
L
|
A
|
L
|
L
|
P
|
L
|
V
|
558 |
ECL2 | |||||||||
G
|
I
|
S
|
S
|
Y
|
A
|
K
|
V
|
S
|
I
|
568 |
TM5 | |||||||||
C
|
L
|
P
|
M
|
D
|
T
|
E
|
T
|
P
|
L
|
578 |
A
|
L
|
A
|
Y
|
I
|
V
|
F
|
V
|
L
|
T
|
588 |
L
|
N
|
I
|
V
|
A
|
F
|
V
|
I
|
V
|
C
|
598 |
C
|
C
|
Y
|
V
|
K
|
I
|
Y
|
I
|
T
|
V
|
608 |
ICL3 | TM6 | ||||||||
R
|
N
|
P
|
Q
|
Y
|
N
|
P
|
G
|
D
|
K
|
618 |
D
|
T
|
K
|
I
|
A
|
K
|
R
|
M
|
A
|
V
|
628 |
L
|
I
|
F
|
T
|
D
|
F
|
I
|
C
|
M
|
A
|
638 |
P
|
I
|
S
|
F
|
Y
|
A
|
L
|
S
|
A
|
I
|
648 |
ECL3 | TM7 | ||||||||
L
|
N
|
K
|
P
|
L
|
I
|
T
|
V
|
S
|
N
|
658 |
S
|
K
|
I
|
L
|
L
|
V
|
L
|
F
|
Y
|
P
|
668 |
L
|
N
|
S
|
C
|
A
|
N
|
P
|
F
|
L
|
Y
|
678 |
H8 | |||||||||
A
|
I
|
F
|
T
|
K
|
A
|
F
|
Q
|
R
|
D
|
688 |
V
|
F
|
I
|
L
|
L
|
S
|
K
|
F
|
G
|
I
|
698 |
C-term | |||||||||
C
|
K
|
R
|
Q
|
A
|
Q
|
A
|
Y
|
R
|
G
|
708 |
Q
|
R
|
V
|
P
|
P
|
K
|
N
|
S
|
T
|
D
|
718 |
I
|
Q
|
V
|
Q
|
K
|
V
|
T
|
H
|
D
|
M
|
728 |
R
|
Q
|
G
|
L
|
H
|
N
|
M
|
E
|
D
|
V
|
738 |
Y
|
E
|
L
|
I
|
E
|
N
|
S
|
H
|
L
|
T
|
748 |
P
|
K
|
K
|
Q
|
G
|
Q
|
I
|
S
|
E
|
E
|
758 |
Y
|
M
|
Q
|
T
|
V
|
L
|
764 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
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|
|
|
|
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|
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|
|
|
|
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|
|
|
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|
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|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|