agre5_human
NAME
7YDP
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
agre5_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
|
G
|
G
|
R
|
V
|
F
|
L
|
A
|
F
|
C
|
10 |
|
|
|
|
|
|
|
|
|
|
V
|
W
|
L
|
T
|
L
|
P
|
G
|
A
|
E
|
T
|
20 |
|
|
|
|
|
|
|
|
|
|
Q
|
D
|
S
|
R
|
G
|
C
|
A
|
R
|
W
|
C
|
30 |
|
|
|
|
|
|
|
|
|
|
P
|
Q
|
N
|
S
|
S
|
C
|
V
|
N
|
A
|
T
|
40 |
|
|
|
|
|
|
|
|
|
|
A
|
C
|
R
|
C
|
N
|
P
|
G
|
F
|
S
|
S
|
50 |
|
|
|
|
|
|
|
|
|
|
F
|
S
|
E
|
I
|
I
|
T
|
T
|
P
|
T
|
E
|
60 |
|
|
|
|
|
|
|
|
|
|
T
|
C
|
D
|
D
|
I
|
N
|
E
|
C
|
A
|
T
|
70 |
|
|
|
|
|
|
|
|
|
|
P
|
S
|
K
|
V
|
S
|
C
|
G
|
K
|
F
|
S
|
80 |
|
|
|
|
|
|
|
|
|
|
D
|
C
|
W
|
N
|
T
|
E
|
G
|
S
|
Y
|
D
|
90 |
|
|
|
|
|
|
|
|
|
|
C
|
V
|
C
|
S
|
P
|
G
|
Y
|
E
|
P
|
V
|
100 |
|
|
|
|
|
|
|
|
|
|
S
|
G
|
A
|
K
|
T
|
F
|
K
|
N
|
E
|
S
|
110 |
|
|
|
|
|
|
|
|
|
|
E
|
N
|
T
|
C
|
Q
|
D
|
V
|
D
|
E
|
C
|
120 |
|
|
|
|
|
|
|
|
|
|
Q
|
Q
|
N
|
P
|
R
|
L
|
C
|
K
|
S
|
Y
|
130 |
|
|
|
|
|
|
|
|
|
|
G
|
T
|
C
|
V
|
N
|
T
|
L
|
G
|
S
|
Y
|
140 |
|
|
|
|
|
|
|
|
|
|
T
|
C
|
Q
|
C
|
L
|
P
|
G
|
F
|
K
|
F
|
150 |
|
|
|
|
|
|
|
|
|
|
I
|
P
|
E
|
D
|
P
|
K
|
V
|
C
|
T
|
D
|
160 |
|
|
|
|
|
|
|
|
|
|
V
|
N
|
E
|
C
|
T
|
S
|
G
|
Q
|
N
|
P
|
170 |
|
|
|
|
|
|
|
|
|
|
C
|
H
|
S
|
S
|
T
|
H
|
C
|
L
|
N
|
N
|
180 |
|
|
|
|
|
|
|
|
|
|
V
|
G
|
S
|
Y
|
Q
|
C
|
R
|
C
|
R
|
P
|
190 |
|
|
|
|
|
|
|
|
|
|
G
|
W
|
Q
|
P
|
I
|
P
|
G
|
S
|
P
|
N
|
200 |
|
|
|
|
|
|
|
|
|
|
G
|
P
|
N
|
N
|
T
|
V
|
C
|
E
|
D
|
V
|
210 |
|
|
|
|
|
|
|
|
|
|
D
|
E
|
C
|
S
|
S
|
G
|
Q
|
H
|
Q
|
C
|
220 |
|
|
|
|
|
|
|
|
|
|
D
|
S
|
S
|
T
|
V
|
C
|
F
|
N
|
T
|
V
|
230 |
|
|
|
|
|
|
|
|
|
|
G
|
S
|
Y
|
S
|
C
|
R
|
C
|
R
|
P
|
G
|
240 |
|
|
|
|
|
|
|
|
|
|
W
|
K
|
P
|
R
|
H
|
G
|
I
|
P
|
N
|
N
|
250 |
|
|
|
|
|
|
|
|
|
|
Q
|
K
|
D
|
T
|
V
|
C
|
E
|
D
|
M
|
T
|
260 |
|
|
|
|
|
|
|
|
|
|
F
|
S
|
T
|
W
|
T
|
P
|
P
|
P
|
G
|
V
|
270 |
|
|
|
|
|
|
|
|
|
|
H
|
S
|
Q
|
T
|
L
|
S
|
R
|
F
|
F
|
D
|
280 |
|
|
|
|
|
|
|
|
|
|
K
|
V
|
Q
|
D
|
L
|
G
|
R
|
D
|
S
|
K
|
290 |
|
|
|
|
|
|
|
|
|
|
T
|
S
|
S
|
A
|
E
|
V
|
T
|
I
|
Q
|
N
|
300 |
|
|
|
|
|
|
|
|
|
|
V
|
I
|
K
|
L
|
V
|
D
|
E
|
L
|
M
|
E
|
310 |
|
|
|
|
|
|
|
|
|
|
A
|
P
|
G
|
D
|
V
|
E
|
A
|
L
|
A
|
P
|
320 |
|
|
|
|
|
|
|
|
|
|
P
|
V
|
R
|
H
|
L
|
I
|
A
|
T
|
Q
|
L
|
330 |
|
|
|
|
|
|
|
|
|
|
L
|
S
|
N
|
L
|
E
|
D
|
I
|
M
|
R
|
I
|
340 |
|
|
|
|
|
|
|
|
|
|
L
|
A
|
K
|
S
|
L
|
P
|
K
|
G
|
P
|
F
|
350 |
|
|
|
|
|
|
|
|
|
|
T
|
Y
|
I
|
S
|
P
|
S
|
N
|
T
|
E
|
L
|
360 |
|
|
|
|
|
|
|
|
|
|
T
|
L
|
M
|
I
|
Q
|
E
|
R
|
G
|
D
|
K
|
370 |
|
|
|
|
|
|
|
|
|
|
N
|
V
|
T
|
M
|
G
|
Q
|
S
|
S
|
A
|
R
|
380 |
|
|
|
|
|
|
|
|
|
|
M
|
K
|
L
|
N
|
W
|
A
|
V
|
A
|
A
|
G
|
390 |
|
|
|
|
|
|
|
|
|
|
A
|
E
|
D
|
P
|
G
|
P
|
A
|
V
|
A
|
G
|
400 |
|
|
|
|
|
|
|
|
|
|
I
|
L
|
S
|
I
|
Q
|
N
|
M
|
T
|
T
|
L
|
410 |
|
|
|
|
|
|
|
|
|
|
L
|
A
|
N
|
A
|
S
|
L
|
N
|
L
|
H
|
S
|
420 |
|
|
|
|
|
|
|
|
|
|
K
|
K
|
Q
|
A
|
E
|
L
|
E
|
E
|
I
|
Y
|
430 |
|
|
|
|
|
|
|
|
|
|
E
|
S
|
S
|
I
|
R
|
G
|
V
|
Q
|
L
|
R
|
440 |
|
|
|
|
|
|
|
|
|
|
R
|
L
|
S
|
A
|
V
|
N
|
S
|
I
|
F
|
L
|
450 |
|
|
|
|
|
|
|
|
|
|
S
|
H
|
N
|
N
|
T
|
K
|
E
|
L
|
N
|
S
|
460 |
|
|
|
|
|
|
|
|
|
|
P
|
I
|
L
|
F
|
A
|
F
|
S
|
H
|
L
|
E
|
470 |
|
|
|
|
|
|
|
|
|
|
S
|
S
|
D
|
G
|
E
|
A
|
G
|
R
|
D
|
P
|
480 |
|
|
|
|
|
|
|
|
|
|
P
|
A
|
K
|
D
|
V
|
M
|
P
|
G
|
P
|
R
|
490 |
|
|
|
|
|
|
|
|
|
|
Q
|
E
|
L
|
L
|
C
|
A
|
F
|
W
|
K
|
S
|
500 |
|
|
|
|
|
|
|
|
|
|
D
|
S
|
D
|
R
|
G
|
G
|
H
|
W
|
A
|
T
|
510 |
|
|
|
|
|
|
|
|
|
|
E
|
G
|
C
|
Q
|
V
|
L
|
G
|
S
|
K
|
N
|
520 |
|
|
|
|
|
|
|
|
|
|
G
|
S
|
T
|
T
|
C
|
Q
|
C
|
S
|
H
|
L
|
530 |
S
|
S
|
F
|
A
|
I
|
L
|
M
|
A
|
H
|
Y
|
540 |
TM1 | |||||||||
D
|
V
|
E
|
D
|
W
|
K
|
L
|
T
|
L
|
I
|
550 |
T
|
R
|
V
|
G
|
L
|
A
|
L
|
S
|
L
|
F
|
560 |
C
|
L
|
L
|
L
|
C
|
I
|
L
|
T
|
F
|
L
|
570 |
ICL1 | TM2 | ||||||||
L
|
V
|
R
|
P
|
I
|
Q
|
G
|
S
|
R
|
T
|
580 |
T
|
I
|
H
|
L
|
H
|
L
|
C
|
I
|
C
|
L
|
590 |
F
|
V
|
G
|
S
|
T
|
I
|
F
|
L
|
A
|
G
|
600 |
ECL1 | TM3 | ||||||||
I
|
E
|
N
|
E
|
G
|
G
|
Q
|
V
|
G
|
L
|
610 |
R
|
C
|
R
|
L
|
V
|
A
|
G
|
L
|
L
|
H
|
620 |
Y
|
C
|
F
|
L
|
A
|
A
|
F
|
C
|
W
|
M
|
630 |
S
|
L
|
E
|
G
|
L
|
E
|
L
|
Y
|
F
|
L
|
640 |
ICL2 | |||||||||
V
|
V
|
R
|
V
|
F
|
Q
|
G
|
Q
|
G
|
L
|
650 |
TM4 | |||||||||
S
|
T
|
R
|
W
|
L
|
C
|
L
|
I
|
G
|
Y
|
660 |
G
|
V
|
P
|
L
|
L
|
I
|
V
|
G
|
V
|
S
|
670 |
ECL2 | |||||||||
A
|
A
|
I
|
Y
|
S
|
K
|
G
|
Y
|
G
|
R
|
680 |
P
|
R
|
Y
|
C
|
W
|
L
|
D
|
F
|
E
|
Q
|
690 |
TM5 | |||||||||
G
|
F
|
L
|
W
|
S
|
F
|
L
|
G
|
P
|
V
|
700 |
T
|
F
|
I
|
I
|
L
|
C
|
N
|
A
|
V
|
I
|
710 |
F
|
V
|
T
|
T
|
V
|
W
|
K
|
L
|
T
|
Q
|
720 |
ICL3 | |||||||||
K
|
F
|
S
|
E
|
I
|
N
|
P
|
D
|
M
|
K
|
730 |
TM6 | |||||||||
K
|
L
|
K
|
K
|
A
|
R
|
A
|
L
|
T
|
I
|
740 |
T
|
A
|
I
|
A
|
Q
|
L
|
F
|
L
|
L
|
G
|
750 |
C
|
T
|
W
|
V
|
F
|
G
|
L
|
F
|
I
|
F
|
760 |
ECL3 | TM7 | ||||||||
D
|
D
|
R
|
S
|
L
|
V
|
L
|
T
|
Y
|
V
|
770 |
F
|
T
|
I
|
L
|
N
|
C
|
L
|
Q
|
G
|
A
|
780 |
F
|
L
|
Y
|
L
|
L
|
H
|
C
|
L
|
L
|
N
|
790 |
H8 | |||||||||
K
|
K
|
V
|
R
|
E
|
E
|
Y
|
R
|
K
|
W
|
800 |
A
|
C
|
L
|
V
|
A
|
G
|
G
|
S
|
K
|
Y
|
810 |
C-term | |||||||||
S
|
E
|
F
|
T
|
S
|
T
|
T
|
S
|
G
|
T
|
820 |
G
|
H
|
N
|
Q
|
T
|
R
|
A
|
L
|
R
|
A
|
830 |
S
|
E
|
S
|
G
|
I
|
|
|||||||||
N-term | |||||||||
|
S
|
S
|
F
|
A
|
I
|
L
|
M
|
A
|
H
|
539 |
TM1 | |||||||||
Y
|
D
|
V
|
E
|
D
|
W
|
K
|
L
|
T
|
L
|
549 |
I
|
T
|
R
|
V
|
G
|
L
|
A
|
L
|
S
|
L
|
559 |
F
|
C
|
L
|
L
|
L
|
C
|
I
|
L
|
T
|
F
|
569 |
ICL1 | TM2 | ||||||||
L
|
L
|
V
|
R
|
P
|
I
|
Q
|
G
|
S
|
R
|
579 |
T
|
T
|
I
|
H
|
L
|
H
|
L
|
C
|
I
|
C
|
589 |
L
|
F
|
V
|
G
|
S
|
T
|
I
|
F
|
L
|
A
|
599 |
ECL1 | |||||||||
G
|
I
|
E
|
N
|
E
|
G
|
G
|
Q
|
V
|
G
|
609 |
TM3 | |||||||||
L
|
R
|
C
|
R
|
L
|
V
|
A
|
G
|
L
|
L
|
619 |
H
|
Y
|
C
|
F
|
L
|
A
|
A
|
F
|
C
|
W
|
629 |
M
|
S
|
L
|
E
|
G
|
L
|
E
|
L
|
Y
|
F
|
639 |
ICL2 | |||||||||
L
|
V
|
V
|
R
|
V
|
F
|
Q
|
G
|
Q
|
G
|
649 |
TM4 | |||||||||
L
|
S
|
T
|
R
|
W
|
L
|
C
|
L
|
I
|
G
|
659 |
Y
|
G
|
V
|
P
|
L
|
L
|
I
|
V
|
G
|
V
|
669 |
ECL2 | |||||||||
S
|
A
|
A
|
I
|
Y
|
S
|
K
|
G
|
Y
|
G
|
679 |
R
|
P
|
R
|
Y
|
C
|
W
|
L
|
D
|
F
|
E
|
689 |
TM5 | |||||||||
Q
|
G
|
F
|
L
|
W
|
S
|
F
|
L
|
G
|
P
|
699 |
V
|
T
|
F
|
I
|
I
|
L
|
C
|
N
|
A
|
V
|
709 |
I
|
F
|
V
|
T
|
T
|
V
|
W
|
K
|
L
|
T
|
719 |
ICL3 | |||||||||
Q
|
K
|
F
|
S
|
E
|
I
|
N
|
P
|
D
|
M
|
729 |
TM6 | |||||||||
K
|
K
|
L
|
K
|
K
|
A
|
R
|
A
|
L
|
T
|
739 |
I
|
T
|
A
|
I
|
A
|
Q
|
L
|
F
|
L
|
L
|
749 |
G
|
C
|
T
|
W
|
V
|
F
|
G
|
L
|
F
|
I
|
759 |
ECL3 | TM7 | ||||||||
F
|
D
|
D
|
R
|
S
|
L
|
V
|
L
|
T
|
Y
|
769 |
V
|
F
|
T
|
I
|
L
|
N
|
C
|
L
|
Q
|
G
|
779 |
A
|
F
|
L
|
Y
|
L
|
L
|
H
|
C
|
L
|
L
|
789 |
H8 | |||||||||
N
|
K
|
K
|
V
|
R
|
E
|
E
|
Y
|
R
|
K
|
799 |
W
|
A
|
C
|
L
|
V
|
A
|
G
|
G
|
S
|
K
|
809 |
C-term | |||||||||
Y
|
S
|
E
|
F
|
T
|
S
|
T
|
T
|
S
|
G
|
819 |
T
|
G
|
H
|
N
|
Q
|
T
|
R
|
A
|
L
|
R
|
829 |
A
|
S
|
E
|
S
|
G
|
I
|
835 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|