smo_human
NAME
7ZI0
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
smo_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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A
|
A
|
A
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R
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P
|
A
|
R
|
G
|
P
|
10 |
|
|
|
|
|
|
|
|
|
|
E
|
L
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P
|
L
|
L
|
G
|
L
|
L
|
L
|
L
|
20 |
|
|
|
|
|
|
|
|
|
|
L
|
L
|
L
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G
|
D
|
P
|
G
|
R
|
G
|
A
|
30 |
|
|||||||||
A
|
S
|
S
|
G
|
N
|
A
|
T
|
G
|
P
|
G
|
40 |
P
|
R
|
S
|
A
|
G
|
G
|
S
|
A
|
R
|
R
|
50 |
S
|
A
|
A
|
V
|
T
|
G
|
P
|
P
|
P
|
P
|
60 |
L
|
S
|
H
|
C
|
G
|
R
|
A
|
A
|
P
|
C
|
70 |
E
|
P
|
L
|
R
|
Y
|
N
|
V
|
C
|
L
|
G
|
80 |
S
|
V
|
L
|
P
|
Y
|
G
|
A
|
T
|
S
|
T
|
90 |
L
|
L
|
A
|
G
|
D
|
S
|
D
|
S
|
Q
|
E
|
100 |
E
|
A
|
H
|
G
|
K
|
L
|
V
|
L
|
W
|
S
|
110 |
G
|
L
|
R
|
N
|
A
|
P
|
R
|
C
|
W
|
A
|
120 |
V
|
I
|
Q
|
P
|
L
|
L
|
C
|
A
|
V
|
Y
|
130 |
M
|
P
|
K
|
C
|
E
|
N
|
D
|
R
|
V
|
E
|
140 |
L
|
P
|
S
|
R
|
T
|
L
|
C
|
Q
|
A
|
T
|
150 |
R
|
G
|
P
|
C
|
A
|
I
|
V
|
E
|
R
|
E
|
160 |
R
|
G
|
W
|
P
|
D
|
F
|
L
|
R
|
C
|
T
|
170 |
P
|
D
|
R
|
F
|
P
|
E
|
G
|
C
|
T
|
N
|
180 |
E
|
V
|
Q
|
N
|
I
|
K
|
F
|
N
|
S
|
S
|
190 |
G
|
Q
|
C
|
E
|
V
|
P
|
L
|
V
|
R
|
T
|
200 |
D
|
N
|
P
|
K
|
S
|
W
|
Y
|
E
|
D
|
V
|
210 |
E
|
G
|
C
|
G
|
I
|
Q
|
C
|
Q
|
N
|
P
|
220 |
TM1 | |||||||||
L
|
F
|
T
|
E
|
A
|
E
|
H
|
Q
|
D
|
M
|
230 |
H
|
S
|
Y
|
I
|
A
|
A
|
F
|
G
|
A
|
V
|
240 |
T
|
G
|
L
|
C
|
T
|
L
|
F
|
T
|
L
|
A
|
250 |
ICL1 | |||||||||
T
|
F
|
V
|
A
|
D
|
W
|
R
|
N
|
S
|
N
|
260 |
TM2 | |||||||||
R
|
Y
|
P
|
A
|
V
|
I
|
L
|
F
|
Y
|
V
|
270 |
N
|
A
|
C
|
F
|
F
|
V
|
G
|
S
|
I
|
G
|
280 |
ECL1 | |||||||||
W
|
L
|
A
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Q
|
F
|
M
|
D
|
G
|
A
|
R
|
290 |
R
|
E
|
I
|
V
|
C
|
R
|
A
|
D
|
G
|
T
|
300 |
M
|
R
|
L
|
G
|
E
|
P
|
T
|
S
|
N
|
E
|
310 |
TM3 | |||||||||
T
|
L
|
S
|
C
|
V
|
I
|
I
|
F
|
V
|
I
|
320 |
V
|
Y
|
Y
|
A
|
L
|
M
|
A
|
G
|
V
|
V
|
330 |
W
|
F
|
V
|
V
|
L
|
T
|
Y
|
A
|
W
|
H
|
340 |
ICL2 | |||||||||
T
|
S
|
F
|
K
|
A
|
L
|
G
|
T
|
T
|
Y
|
350 |
TM4 | |||||||||
Q
|
P
|
L
|
S
|
G
|
K
|
T
|
S
|
Y
|
F
|
360 |
H
|
L
|
L
|
T
|
W
|
S
|
L
|
P
|
F
|
V
|
370 |
L
|
T
|
V
|
A
|
I
|
L
|
A
|
V
|
A
|
Q
|
380 |
ECL2 | |||||||||
V
|
D
|
G
|
D
|
S
|
V
|
S
|
G
|
I
|
C
|
390 |
TM5 | |||||||||
F
|
V
|
G
|
Y
|
K
|
N
|
Y
|
R
|
Y
|
R
|
400 |
A
|
G
|
F
|
V
|
L
|
A
|
P
|
I
|
G
|
L
|
410 |
V
|
L
|
I
|
V
|
G
|
G
|
Y
|
F
|
L
|
I
|
420 |
|
|
||||||||
R
|
G
|
V
|
M
|
T
|
L
|
F
|
S
|
I
|
K
|
430 |
|
|
|
|
|
|
|
|
|
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ICL3 | TM6 | ||||||||
S
|
N
|
H
|
P
|
G
|
L
|
L
|
S
|
E
|
K
|
440 |
|
|
||||||||
A
|
A
|
S
|
K
|
I
|
N
|
E
|
T
|
M
|
L
|
450 |
R
|
L
|
G
|
I
|
F
|
G
|
F
|
L
|
A
|
F
|
460 |
G
|
F
|
V
|
L
|
I
|
T
|
F
|
S
|
C
|
H
|
470 |
F
|
Y
|
D
|
F
|
F
|
N
|
Q
|
A
|
E
|
W
|
480 |
E
|
R
|
S
|
F
|
R
|
D
|
Y
|
V
|
L
|
C
|
490 |
ECL3 | |||||||||
Q
|
A
|
N
|
V
|
T
|
I
|
G
|
L
|
P
|
T
|
500 |
K
|
Q
|
P
|
I
|
P
|
D
|
C
|
E
|
I
|
K
|
510 |
TM7 | |||||||||
N
|
R
|
P
|
S
|
L
|
L
|
V
|
E
|
K
|
I
|
520 |
N
|
L
|
F
|
A
|
M
|
F
|
G
|
T
|
G
|
I
|
530 |
H8 | |||||||||
A
|
M
|
S
|
T
|
W
|
V
|
W
|
T
|
K
|
A
|
540 |
T
|
L
|
L
|
I
|
W
|
R
|
R
|
T
|
W
|
C
|
550 |
|
|
|
|
|
|||||
C-term | |||||||||
R
|
L
|
T
|
G
|
Q
|
S
|
D
|
D
|
E
|
P
|
560 |
|
|
|
|
|
|
|
|
|
|
K
|
R
|
I
|
K
|
K
|
S
|
K
|
M
|
I
|
A
|
570 |
|
|
|
|
|
|
|
|
|
|
K
|
A
|
F
|
S
|
K
|
R
|
H
|
E
|
L
|
L
|
580 |
|
|
|
|
|
|
|
|
|
|
Q
|
N
|
P
|
G
|
Q
|
E
|
L
|
S
|
F
|
S
|
590 |
|
|
|
|
|
|
|
|
|
|
M
|
H
|
T
|
V
|
S
|
H
|
D
|
G
|
P
|
V
|
600 |
|
|
|
|
|
|
|
|
|
|
A
|
G
|
L
|
A
|
F
|
D
|
L
|
N
|
E
|
P
|
610 |
|
|
|
|
|
|
|
|
|
|
S
|
A
|
D
|
V
|
S
|
S
|
A
|
W
|
A
|
Q
|
620 |
|
|
|
|
|
|
|
|
|
|
H
|
V
|
T
|
K
|
M
|
V
|
A
|
R
|
R
|
G
|
630 |
|
|
|
|
|
|
|
|
|
|
A
|
I
|
L
|
P
|
Q
|
D
|
I
|
S
|
V
|
T
|
640 |
|
|
|
|
|
|
|
|
|
|
P
|
V
|
A
|
T
|
P
|
V
|
P
|
P
|
E
|
E
|
650 |
|
|
|
|
|
|
|
|
|
|
Q
|
A
|
N
|
L
|
W
|
L
|
V
|
E
|
A
|
E
|
660 |
|
|
|
|
|
|
|
|
|
|
I
|
S
|
P
|
E
|
L
|
Q
|
K
|
R
|
L
|
G
|
670 |
|
|
|
|
|
|
|
|
|
|
R
|
K
|
K
|
K
|
R
|
R
|
K
|
R
|
K
|
K
|
680 |
|
|
|
|
|
|
|
|
|
|
E
|
V
|
C
|
P
|
L
|
A
|
P
|
P
|
P
|
E
|
690 |
|
|
|
|
|
|
|
|
|
|
L
|
H
|
P
|
P
|
A
|
P
|
A
|
P
|
S
|
T
|
700 |
|
|
|
|
|
|
|
|
|
|
I
|
P
|
R
|
L
|
P
|
Q
|
L
|
P
|
R
|
Q
|
710 |
|
|
|
|
|
|
|
|
|
|
K
|
C
|
L
|
V
|
A
|
A
|
G
|
A
|
W
|
G
|
720 |
|
|
|
|
|
|
|
|
|
|
A
|
G
|
D
|
S
|
C
|
R
|
Q
|
G
|
A
|
W
|
730 |
|
|
|
|
|
|
|
|
|
|
T
|
L
|
V
|
S
|
N
|
P
|
F
|
C
|
P
|
E
|
740 |
|
|
|
|
|
|
|
|
|
|
P
|
S
|
P
|
P
|
Q
|
D
|
P
|
F
|
L
|
P
|
750 |
|
|
|
|
|
|
|
|
|
|
S
|
A
|
P
|
A
|
P
|
V
|
A
|
W
|
A
|
H
|
760 |
|
|
|
|
|
|
|
|
|
|
G
|
R
|
R
|
Q
|
G
|
L
|
G
|
P
|
I
|
H
|
770 |
|
|
|
|
|
|
|
|
|
|
S
|
R
|
T
|
N
|
L
|
M
|
D
|
T
|
E
|
L
|
780 |
|
|
|
|
|
|
|
M
|
D
|
A
|
D
|
S
|
D
|
F
|
|
|||||||||
N-term | |||||||||
|
S
|
S
|
G
|
N
|
A
|
T
|
G
|
P
|
G
|
40 |
P
|
R
|
S
|
A
|
G
|
G
|
S
|
A
|
R
|
R
|
50 |
S
|
A
|
A
|
V
|
T
|
G
|
P
|
P
|
P
|
P
|
60 |
L
|
S
|
H
|
C
|
G
|
R
|
A
|
A
|
P
|
C
|
70 |
E
|
P
|
L
|
R
|
Y
|
N
|
V
|
C
|
L
|
G
|
80 |
S
|
V
|
L
|
P
|
Y
|
G
|
A
|
T
|
S
|
T
|
90 |
L
|
L
|
A
|
G
|
D
|
S
|
D
|
S
|
Q
|
E
|
100 |
E
|
A
|
H
|
G
|
K
|
L
|
V
|
L
|
W
|
S
|
110 |
G
|
L
|
R
|
N
|
A
|
P
|
R
|
C
|
W
|
A
|
120 |
V
|
I
|
Q
|
P
|
L
|
L
|
C
|
A
|
V
|
Y
|
130 |
M
|
P
|
K
|
C
|
E
|
N
|
D
|
R
|
V
|
E
|
140 |
L
|
P
|
S
|
R
|
T
|
L
|
C
|
Q
|
A
|
T
|
150 |
R
|
G
|
P
|
C
|
A
|
I
|
V
|
E
|
R
|
E
|
160 |
R
|
G
|
W
|
P
|
D
|
F
|
L
|
R
|
C
|
T
|
170 |
P
|
D
|
R
|
F
|
P
|
E
|
G
|
C
|
T
|
N
|
180 |
E
|
V
|
Q
|
N
|
I
|
K
|
F
|
N
|
S
|
S
|
190 |
G
|
Q
|
C
|
E
|
V
|
P
|
L
|
V
|
R
|
T
|
200 |
D
|
N
|
P
|
K
|
S
|
W
|
Y
|
E
|
D
|
V
|
210 |
E
|
G
|
C
|
G
|
I
|
Q
|
C
|
Q
|
N
|
P
|
220 |
TM1 | |||||||||
L
|
F
|
T
|
E
|
A
|
E
|
H
|
Q
|
D
|
M
|
230 |
H
|
S
|
Y
|
I
|
A
|
A
|
F
|
G
|
A
|
V
|
240 |
T
|
G
|
L
|
C
|
T
|
L
|
F
|
T
|
L
|
A
|
250 |
ICL1 | |||||||||
T
|
F
|
V
|
A
|
D
|
W
|
R
|
N
|
S
|
N
|
260 |
TM2 | |||||||||
R
|
Y
|
P
|
A
|
V
|
I
|
L
|
F
|
Y
|
V
|
270 |
N
|
A
|
C
|
F
|
F
|
V
|
G
|
S
|
I
|
G
|
280 |
ECL1 | |||||||||
W
|
L
|
A
|
Q
|
F
|
M
|
D
|
G
|
A
|
R
|
290 |
R
|
E
|
I
|
V
|
C
|
R
|
A
|
D
|
G
|
T
|
300 |
M
|
R
|
L
|
G
|
E
|
P
|
T
|
S
|
N
|
E
|
310 |
TM3 | |||||||||
T
|
L
|
S
|
C
|
V
|
I
|
I
|
F
|
V
|
I
|
320 |
V
|
Y
|
Y
|
A
|
L
|
M
|
A
|
G
|
V
|
V
|
330 |
W
|
F
|
V
|
V
|
L
|
T
|
Y
|
A
|
W
|
H
|
340 |
ICL2 | |||||||||
T
|
S
|
F
|
K
|
A
|
L
|
G
|
T
|
T
|
Y
|
350 |
TM4 | |||||||||
Q
|
P
|
L
|
S
|
G
|
K
|
T
|
S
|
Y
|
F
|
360 |
H
|
L
|
L
|
T
|
W
|
S
|
L
|
P
|
F
|
V
|
370 |
L
|
T
|
V
|
A
|
I
|
L
|
A
|
V
|
A
|
Q
|
380 |
ECL2 | |||||||||
V
|
D
|
G
|
D
|
S
|
V
|
S
|
G
|
I
|
C
|
390 |
TM5 | |||||||||
F
|
V
|
G
|
Y
|
K
|
N
|
Y
|
R
|
Y
|
R
|
400 |
A
|
G
|
F
|
V
|
L
|
A
|
P
|
I
|
G
|
L
|
410 |
V
|
L
|
I
|
V
|
G
|
G
|
Y
|
F
|
L
|
I
|
420 |
|
|||||||||
R
|
G
|
V
|
M
|
T
|
L
|
F
|
S
|
|
S
|
443 |
TM6 | |||||||||
K
|
I
|
N
|
E
|
T
|
M
|
L
|
R
|
L
|
G
|
453 |
I
|
F
|
G
|
F
|
L
|
A
|
F
|
G
|
F
|
V
|
463 |
L
|
I
|
T
|
F
|
S
|
C
|
H
|
F
|
Y
|
D
|
473 |
F
|
F
|
N
|
Q
|
A
|
E
|
W
|
E
|
R
|
S
|
483 |
F
|
R
|
D
|
Y
|
V
|
L
|
C
|
Q
|
A
|
N
|
493 |
ECL3 | |||||||||
V
|
T
|
I
|
G
|
L
|
P
|
T
|
K
|
Q
|
P
|
503 |
I
|
P
|
D
|
C
|
E
|
I
|
K
|
N
|
R
|
P
|
513 |
TM7 | |||||||||
S
|
L
|
L
|
V
|
E
|
K
|
I
|
N
|
L
|
F
|
523 |
A
|
M
|
F
|
G
|
T
|
G
|
I
|
A
|
M
|
S
|
533 |
H8 | |||||||||
T
|
W
|
V
|
W
|
T
|
K
|
A
|
T
|
L
|
L
|
543 |
I
|
W
|
R
|
R
|
T
|
W
|
C
|
R
|
L
|
T
|
553 |
|
|||||
C-term | |||||
G
|
Q
|
|
|||
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|