pth1r_human
NAME
8FLR
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
pth1r_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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G
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T
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A
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R
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I
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A
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P
|
G
|
L
|
10 |
|
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|
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A
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L
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L
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L
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C
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C
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P
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V
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L
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S
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20 |
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|||
S
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A
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Y
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A
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L
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V
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D
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A
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D
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D
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30 |
V
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M
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T
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K
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E
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E
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Q
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I
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F
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L
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40 |
L
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A
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Q
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A
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Q
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C
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E
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K
|
50 |
R
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L
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K
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L
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Q
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R
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P
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A
|
60 |
S
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I
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M
|
E
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S
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D
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K
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G
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W
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T
|
70 |
S
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S
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S
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G
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K
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P
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R
|
K
|
80 |
D
|
K
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A
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S
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G
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K
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L
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Y
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P
|
E
|
90 |
S
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E
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D
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K
|
E
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A
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P
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T
|
G
|
100 |
S
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R
|
Y
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R
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G
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R
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P
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C
|
L
|
P
|
110 |
E
|
W
|
D
|
H
|
I
|
L
|
C
|
W
|
P
|
L
|
120 |
G
|
A
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P
|
G
|
E
|
V
|
V
|
A
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V
|
P
|
130 |
C
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P
|
D
|
Y
|
I
|
Y
|
D
|
F
|
N
|
H
|
140 |
K
|
G
|
H
|
A
|
Y
|
R
|
R
|
C
|
D
|
R
|
150 |
N
|
G
|
S
|
W
|
E
|
L
|
V
|
P
|
G
|
H
|
160 |
N
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R
|
T
|
W
|
A
|
N
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Y
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S
|
E
|
C
|
170 |
TM1 | |||||||||
V
|
K
|
F
|
L
|
T
|
N
|
E
|
T
|
R
|
E
|
180 |
R
|
E
|
V
|
F
|
D
|
R
|
L
|
G
|
M
|
I
|
190 |
Y
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T
|
V
|
G
|
Y
|
S
|
V
|
S
|
L
|
A
|
200 |
S
|
L
|
T
|
V
|
A
|
V
|
L
|
I
|
L
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A
|
210 |
ICL1 | TM2 | ||||||||
Y
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F
|
R
|
R
|
L
|
H
|
C
|
T
|
R
|
N
|
220 |
Y
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I
|
H
|
M
|
H
|
L
|
F
|
L
|
S
|
F
|
230 |
M
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L
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R
|
A
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V
|
S
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I
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F
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V
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K
|
240 |
D
|
A
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V
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L
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Y
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S
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G
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A
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T
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L
|
250 |
ECL1 | |||||||||
D
|
E
|
A
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E
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R
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L
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T
|
E
|
E
|
E
|
260 |
L
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R
|
A
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I
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A
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Q
|
A
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P
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P
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P
|
270 |
TM3 | |||||||||
P
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A
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T
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A
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A
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A
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G
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Y
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A
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G
|
280 |
C
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R
|
V
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A
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V
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T
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F
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F
|
L
|
Y
|
290 |
F
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L
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A
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T
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N
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Y
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Y
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W
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I
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L
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300 |
V
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E
|
G
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L
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Y
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L
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H
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S
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L
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I
|
310 |
ICL2 | TM4 | ||||||||
F
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M
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A
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F
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F
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S
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E
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K
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K
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Y
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320 |
L
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W
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G
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F
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T
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V
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F
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G
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W
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G
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330 |
L
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P
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A
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V
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F
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V
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A
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V
|
W
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V
|
340 |
S
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V
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R
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A
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T
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L
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A
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N
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T
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G
|
350 |
ECL2 | TM5 | ||||||||
C
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W
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D
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L
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S
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S
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G
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N
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K
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K
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360 |
W
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I
|
I
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Q
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V
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P
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I
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L
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A
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S
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370 |
I
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V
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L
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N
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F
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I
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L
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F
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I
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N
|
380 |
I
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V
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R
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V
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L
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A
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T
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K
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L
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R
|
390 |
ICL3 | TM6 | ||||||||
E
|
T
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N
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A
|
G
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R
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C
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D
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T
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R
|
400 |
Q
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Q
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Y
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R
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K
|
L
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L
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K
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S
|
T
|
410 |
L
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V
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L
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M
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P
|
L
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F
|
G
|
V
|
H
|
420 |
Y
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I
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V
|
F
|
M
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A
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T
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P
|
Y
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T
|
430 |
ECL3 | TM7 | ||||||||
E
|
V
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S
|
G
|
T
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L
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W
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Q
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V
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Q
|
440 |
M
|
H
|
Y
|
E
|
M
|
L
|
F
|
N
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S
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F
|
450 |
Q
|
G
|
F
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F
|
V
|
A
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I
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I
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Y
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C
|
460 |
H8 | |||||||||
F
|
C
|
N
|
G
|
E
|
V
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Q
|
A
|
E
|
I
|
470 |
K
|
K
|
S
|
W
|
S
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R
|
W
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T
|
L
|
A
|
480 |
L
|
D
|
F
|
K
|
R
|
K
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A
|
R
|
S
|
G
|
490 |
C-term | |||||||||
S
|
S
|
S
|
Y
|
S
|
Y
|
G
|
P
|
M
|
V
|
500 |
S
|
H
|
T
|
S
|
V
|
T
|
N
|
V
|
G
|
P
|
510 |
R
|
V
|
G
|
L
|
G
|
L
|
P
|
L
|
S
|
P
|
520 |
R
|
L
|
L
|
P
|
T
|
A
|
T
|
T
|
N
|
G
|
530 |
H
|
P
|
Q
|
L
|
P
|
G
|
H
|
A
|
K
|
P
|
540 |
G
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T
|
P
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A
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L
|
E
|
T
|
L
|
E
|
T
|
550 |
T
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P
|
P
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A
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M
|
A
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A
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P
|
K
|
D
|
560 |
D
|
G
|
F
|
L
|
N
|
G
|
S
|
C
|
S
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G
|
570 |
L
|
D
|
E
|
E
|
A
|
S
|
G
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P
|
E
|
R
|
580 |
P
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P
|
A
|
L
|
L
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Q
|
E
|
E
|
W
|
E
|
590 |
T
|
V
|
M
|
|
|||||||||
N-term | |||||||||
|
A
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D
|
D
|
V
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M
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T
|
K
|
E
|
E
|
36 |
Q
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I
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F
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L
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L
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H
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R
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A
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Q
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A
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46 |
Q
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C
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E
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K
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R
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L
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K
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E
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V
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L
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56 |
Q
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R
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P
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A
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S
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I
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M
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E
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S
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D
|
66 |
K
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G
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W
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T
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S
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A
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S
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T
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S
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G
|
76 |
K
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P
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R
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K
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D
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K
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A
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S
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G
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K
|
86 |
L
|
Y
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P
|
E
|
S
|
E
|
E
|
D
|
K
|
E
|
96 |
A
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P
|
T
|
G
|
S
|
R
|
Y
|
R
|
G
|
R
|
106 |
P
|
C
|
L
|
P
|
E
|
W
|
D
|
H
|
I
|
L
|
116 |
C
|
W
|
P
|
L
|
G
|
A
|
P
|
G
|
E
|
V
|
126 |
V
|
A
|
V
|
P
|
C
|
P
|
D
|
Y
|
I
|
Y
|
136 |
D
|
F
|
N
|
H
|
K
|
G
|
H
|
A
|
Y
|
R
|
146 |
R
|
C
|
D
|
R
|
N
|
G
|
S
|
W
|
E
|
L
|
156 |
V
|
P
|
G
|
H
|
N
|
R
|
T
|
W
|
A
|
N
|
166 |
TM1 | |||||||||
Y
|
S
|
E
|
C
|
V
|
K
|
F
|
L
|
T
|
N
|
176 |
E
|
T
|
R
|
E
|
R
|
E
|
V
|
F
|
D
|
R
|
186 |
L
|
G
|
M
|
I
|
Y
|
T
|
V
|
G
|
Y
|
S
|
196 |
V
|
S
|
L
|
A
|
S
|
L
|
T
|
V
|
A
|
V
|
206 |
ICL1 | |||||||||
L
|
I
|
L
|
A
|
Y
|
F
|
R
|
R
|
L
|
H
|
216 |
TM2 | |||||||||
C
|
T
|
R
|
N
|
Y
|
I
|
H
|
M
|
H
|
L
|
226 |
F
|
L
|
S
|
F
|
M
|
L
|
R
|
A
|
V
|
S
|
236 |
I
|
F
|
V
|
K
|
D
|
A
|
V
|
L
|
Y
|
S
|
246 |
ECL1 | |||||||||
G
|
A
|
T
|
L
|
D
|
E
|
A
|
E
|
R
|
L
|
256 |
T
|
E
|
E
|
E
|
L
|
R
|
A
|
I
|
A
|
Q
|
266 |
A
|
P
|
P
|
P
|
P
|
A
|
T
|
A
|
A
|
A
|
276 |
TM3 | |||||||||
G
|
Y
|
A
|
G
|
C
|
R
|
V
|
A
|
V
|
T
|
286 |
F
|
F
|
L
|
Y
|
F
|
L
|
A
|
T
|
N
|
Y
|
296 |
Y
|
W
|
I
|
L
|
V
|
E
|
G
|
L
|
Y
|
L
|
306 |
ICL2 | |||||||||
H
|
S
|
L
|
I
|
F
|
M
|
A
|
F
|
F
|
S
|
316 |
TM4 | |||||||||
E
|
K
|
K
|
Y
|
L
|
W
|
G
|
F
|
T
|
V
|
326 |
F
|
G
|
W
|
G
|
L
|
P
|
A
|
V
|
F
|
V
|
336 |
A
|
V
|
W
|
V
|
S
|
V
|
R
|
A
|
T
|
L
|
346 |
ECL2 | |||||||||
A
|
N
|
T
|
G
|
C
|
W
|
D
|
L
|
S
|
S
|
356 |
TM5 | |||||||||
G
|
N
|
K
|
K
|
W
|
I
|
I
|
Q
|
V
|
P
|
366 |
I
|
L
|
A
|
S
|
I
|
V
|
L
|
N
|
F
|
I
|
376 |
L
|
F
|
I
|
N
|
I
|
V
|
R
|
V
|
L
|
A
|
386 |
ICL3 | |||||||||
T
|
K
|
L
|
R
|
E
|
T
|
N
|
A
|
G
|
R
|
396 |
TM6 | |||||||||
C
|
D
|
T
|
R
|
Q
|
Q
|
Y
|
R
|
K
|
L
|
406 |
L
|
K
|
S
|
T
|
L
|
V
|
L
|
M
|
P
|
L
|
416 |
F
|
G
|
V
|
H
|
Y
|
I
|
V
|
F
|
M
|
A
|
426 |
ECL3 | TM7 | ||||||||
T
|
P
|
Y
|
T
|
E
|
V
|
S
|
G
|
T
|
L
|
436 |
W
|
Q
|
V
|
Q
|
M
|
H
|
Y
|
E
|
M
|
L
|
446 |
F
|
N
|
S
|
F
|
Q
|
G
|
F
|
F
|
V
|
A
|
456 |
H8 | |||||||||
I
|
I
|
Y
|
C
|
F
|
C
|
N
|
G
|
E
|
V
|
466 |
Q
|
A
|
E
|
I
|
K
|
K
|
S
|
W
|
S
|
R
|
476 |
W
|
T
|
L
|
A
|
L
|
D
|
F
|
K
|
R
|
K
|
486 |
C-term | |||||||||
A
|
R
|
S
|
G
|
S
|
S
|
S
|
Y
|
S
|
Y
|
496 |
G
|
P
|
M
|
V
|
S
|
H
|
T
|
S
|
V
|
T
|
506 |
N
|
V
|
G
|
P
|
R
|
V
|
G
|
L
|
G
|
L
|
516 |
P
|
L
|
S
|
P
|
R
|
L
|
L
|
P
|
T
|
A
|
526 |
T
|
T
|
N
|
G
|
H
|
P
|
Q
|
L
|
P
|
G
|
536 |
H
|
A
|
K
|
P
|
G
|
T
|
P
|
A
|
L
|
E
|
546 |
T
|
L
|
E
|
T
|
T
|
P
|
P
|
A
|
M
|
A
|
556 |
A
|
P
|
K
|
D
|
D
|
G
|
F
|
L
|
N
|
G
|
566 |
S
|
C
|
S
|
G
|
L
|
D
|
E
|
E
|
A
|
S
|
576 |
G
|
P
|
E
|
R
|
P
|
P
|
A
|
L
|
L
|
Q
|
586 |
E
|
E
|
W
|
E
|
T
|
V
|
M
|
593 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|