agrf1_human
NAME
8G2Y
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
agrf1_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
K
|
V
|
G
|
V
|
L
|
W
|
L
|
I
|
S
|
10 |
F
|
F
|
T
|
F
|
T
|
D
|
G
|
H
|
G
|
G
|
20 |
F
|
L
|
G
|
K
|
N
|
D
|
G
|
I
|
K
|
T
|
30 |
K
|
K
|
E
|
L
|
I
|
V
|
N
|
K
|
K
|
K
|
40 |
H
|
L
|
G
|
P
|
V
|
E
|
E
|
Y
|
Q
|
L
|
50 |
L
|
L
|
Q
|
V
|
T
|
Y
|
R
|
D
|
S
|
K
|
60 |
E
|
K
|
R
|
D
|
L
|
R
|
N
|
F
|
L
|
K
|
70 |
L
|
L
|
K
|
P
|
P
|
L
|
L
|
W
|
S
|
H
|
80 |
G
|
L
|
I
|
R
|
I
|
I
|
R
|
A
|
K
|
A
|
90 |
T
|
T
|
D
|
C
|
N
|
S
|
L
|
N
|
G
|
V
|
100 |
L
|
Q
|
C
|
T
|
C
|
E
|
D
|
S
|
Y
|
T
|
110 |
W
|
F
|
P
|
P
|
S
|
C
|
L
|
D
|
P
|
Q
|
120 |
N
|
C
|
Y
|
L
|
H
|
T
|
A
|
G
|
A
|
L
|
130 |
P
|
S
|
C
|
E
|
C
|
H
|
L
|
N
|
N
|
L
|
140 |
S
|
Q
|
S
|
V
|
N
|
F
|
C
|
E
|
R
|
T
|
150 |
K
|
I
|
W
|
G
|
T
|
F
|
K
|
I
|
N
|
E
|
160 |
R
|
F
|
T
|
N
|
D
|
L
|
L
|
N
|
S
|
S
|
170 |
S
|
A
|
I
|
Y
|
S
|
K
|
Y
|
A
|
N
|
G
|
180 |
I
|
E
|
I
|
Q
|
L
|
K
|
K
|
A
|
Y
|
E
|
190 |
R
|
I
|
Q
|
G
|
F
|
E
|
S
|
V
|
Q
|
V
|
200 |
T
|
Q
|
F
|
R
|
N
|
G
|
S
|
I
|
V
|
A
|
210 |
G
|
Y
|
E
|
V
|
V
|
G
|
S
|
S
|
S
|
A
|
220 |
S
|
E
|
L
|
L
|
S
|
A
|
I
|
E
|
H
|
V
|
230 |
A
|
E
|
K
|
A
|
K
|
T
|
A
|
L
|
H
|
K
|
240 |
L
|
F
|
P
|
L
|
E
|
D
|
G
|
S
|
F
|
R
|
250 |
V
|
F
|
G
|
K
|
A
|
Q
|
C
|
N
|
D
|
I
|
260 |
V
|
F
|
G
|
F
|
G
|
S
|
K
|
D
|
D
|
E
|
270 |
Y
|
T
|
L
|
P
|
C
|
S
|
S
|
G
|
Y
|
R
|
280 |
G
|
N
|
I
|
T
|
A
|
K
|
C
|
E
|
S
|
S
|
290 |
G
|
W
|
Q
|
V
|
I
|
R
|
E
|
T
|
C
|
V
|
300 |
L
|
S
|
L
|
L
|
E
|
E
|
L
|
N
|
K
|
N
|
310 |
F
|
S
|
M
|
I
|
V
|
G
|
N
|
A
|
T
|
E
|
320 |
A
|
A
|
V
|
S
|
S
|
F
|
V
|
Q
|
N
|
L
|
330 |
S
|
V
|
I
|
I
|
R
|
Q
|
N
|
P
|
S
|
T
|
340 |
T
|
V
|
G
|
N
|
L
|
A
|
S
|
V
|
V
|
S
|
350 |
I
|
L
|
S
|
N
|
I
|
S
|
S
|
L
|
S
|
L
|
360 |
A
|
S
|
H
|
F
|
R
|
V
|
S
|
N
|
S
|
T
|
370 |
M
|
E
|
D
|
V
|
I
|
S
|
I
|
A
|
D
|
N
|
380 |
I
|
L
|
N
|
S
|
A
|
S
|
V
|
T
|
N
|
W
|
390 |
|
|
|
|
|
|
||||
T
|
V
|
L
|
L
|
R
|
E
|
E
|
K
|
Y
|
A
|
400 |
|
|
|
|
|
|
|
|
|
|
S
|
S
|
R
|
L
|
L
|
E
|
T
|
L
|
E
|
N
|
410 |
|
|
|
|
|
|
|
|
|
|
I
|
S
|
T
|
L
|
V
|
P
|
P
|
T
|
A
|
L
|
420 |
|
|
|
|
|
|
|
|
|
|
P
|
L
|
N
|
F
|
S
|
R
|
K
|
F
|
I
|
D
|
430 |
|
|
|
|
|
|
|
|
|
|
W
|
K
|
G
|
I
|
P
|
V
|
N
|
K
|
S
|
Q
|
440 |
|
|
|
|
|
|
|
|
|
|
L
|
K
|
R
|
G
|
Y
|
S
|
Y
|
Q
|
I
|
K
|
450 |
|
|
|
|
|
|
|
|
|
|
M
|
C
|
P
|
Q
|
N
|
T
|
S
|
I
|
P
|
I
|
460 |
|
|
|
|
|
|
|
|
|
|
R
|
G
|
R
|
V
|
L
|
I
|
G
|
S
|
D
|
Q
|
470 |
|
|
|
|
|
|
|
|
|
|
F
|
Q
|
R
|
S
|
L
|
P
|
E
|
T
|
I
|
I
|
480 |
|
|
|
|
|
|
|
|
|
|
S
|
M
|
A
|
S
|
L
|
T
|
L
|
G
|
N
|
I
|
490 |
|
|
|
|
|
|
|
|
|
|
L
|
P
|
V
|
S
|
K
|
N
|
G
|
N
|
A
|
Q
|
500 |
|
|
|
|
|
|
|
|
|
|
V
|
N
|
G
|
P
|
V
|
I
|
S
|
T
|
V
|
I
|
510 |
|
|
|
|
|
|
|
|
|
|
Q
|
N
|
Y
|
S
|
I
|
N
|
E
|
V
|
F
|
L
|
520 |
|
|
|
|
|
|
|
|
|
|
F
|
F
|
S
|
K
|
I
|
E
|
S
|
N
|
L
|
S
|
530 |
|
|
|
|
|
|
|
|
|
|
Q
|
P
|
H
|
C
|
V
|
F
|
W
|
D
|
F
|
S
|
540 |
|
|
|
|
|
|
|
|
|
|
H
|
L
|
Q
|
W
|
N
|
D
|
A
|
G
|
C
|
H
|
550 |
|
|
|
|
|
|
|
|
|
|
L
|
V
|
N
|
E
|
T
|
Q
|
D
|
I
|
V
|
T
|
560 |
|
|
|
|
|
|
|
|
|
|
C
|
Q
|
C
|
T
|
H
|
L
|
T
|
S
|
F
|
S
|
570 |
|
|
|
|
|
|
|
|
|
|
TM1 | |||||||||
I
|
L
|
M
|
S
|
P
|
F
|
V
|
P
|
S
|
T
|
580 |
|
|
|
|
|
|
|
|
|
|
I
|
F
|
P
|
V
|
V
|
K
|
W
|
I
|
T
|
Y
|
590 |
|
|
|
|
|
|
|
|
|
|
V
|
G
|
L
|
G
|
I
|
S
|
I
|
G
|
S
|
L
|
600 |
|
|
|
|
|
|
|
|
|
|
I
|
L
|
C
|
L
|
I
|
I
|
E
|
A
|
L
|
F
|
610 |
|
|
|
|
|
|
|
|
|
|
ICL1 | TM2 | ||||||||
W
|
K
|
Q
|
I
|
K
|
K
|
S
|
Q
|
T
|
S
|
620 |
|
|
|
|
|
|
|
|
|
|
H
|
T
|
R
|
R
|
I
|
C
|
M
|
V
|
N
|
I
|
630 |
|
|
|
|
|
|
|
|
|
|
A
|
L
|
S
|
L
|
L
|
I
|
A
|
D
|
V
|
W
|
640 |
|
|
|
|
|
|
|
|
|
|
F
|
I
|
V
|
G
|
A
|
T
|
V
|
D
|
T
|
T
|
650 |
|
|
|
|
|
|
|
|
|
|
ECL1 | TM3 | ||||||||
V
|
N
|
P
|
S
|
G
|
V
|
C
|
T
|
A
|
A
|
660 |
|
|
|
|
|
|
|
|
|
|
V
|
F
|
F
|
T
|
H
|
F
|
F
|
Y
|
L
|
S
|
670 |
|
|
|
|
|
|
|
|
|
|
L
|
F
|
F
|
W
|
M
|
L
|
M
|
L
|
G
|
I
|
680 |
|
|
|
|
|
|
|
|
|
|
L
|
L
|
A
|
Y
|
R
|
I
|
I
|
L
|
V
|
F
|
690 |
|
|
|
|
|
|
|
|
|
|
ICL2 | TM4 | ||||||||
H
|
H
|
M
|
A
|
Q
|
H
|
L
|
M
|
M
|
A
|
700 |
|
|
|
|
|
|
|
|
|
|
V
|
G
|
F
|
C
|
L
|
G
|
Y
|
G
|
C
|
P
|
710 |
|
|
|
|
|
|
|
|
|
|
L
|
I
|
I
|
S
|
V
|
I
|
T
|
I
|
A
|
V
|
720 |
|
|
|
|
|
|
|
|
|
|
ECL2 | |||||||||
T
|
Q
|
P
|
S
|
N
|
T
|
Y
|
K
|
R
|
K
|
730 |
|
|
|
|
|
|
|
|
|
|
D
|
V
|
C
|
W
|
L
|
N
|
W
|
S
|
N
|
G
|
740 |
|
|
|
|
|
|
|
|
|
|
TM5 | |||||||||
S
|
K
|
P
|
L
|
L
|
A
|
F
|
V
|
V
|
P
|
750 |
|
|
|
|
|
|
|
|
|
|
A
|
L
|
A
|
I
|
V
|
A
|
V
|
N
|
F
|
V
|
760 |
|
|
|
|
|
|
|
|
|
|
V
|
V
|
L
|
L
|
V
|
L
|
T
|
K
|
L
|
W
|
770 |
|
|
|
|
|
|
|
|
|
|
ICL3 | |||||||||
R
|
P
|
T
|
V
|
G
|
E
|
R
|
L
|
S
|
R
|
780 |
|
|
|
|
|
|
|
|
|
|
TM6 | |||||||||
D
|
D
|
K
|
A
|
T
|
I
|
I
|
R
|
V
|
G
|
790 |
|
|
|
|
|
|
|
|
|
|
K
|
S
|
L
|
L
|
I
|
L
|
T
|
P
|
L
|
L
|
800 |
|
|
|
|
|
|
|
|
|
|
G
|
L
|
T
|
W
|
G
|
F
|
G
|
I
|
G
|
T
|
810 |
|
|
|
|
|
|
|
|
|
|
|
ECL3 | TM7 | |||||||||
I
|
V
|
D
|
S
|
Q
|
N
|
L
|
A
|
W
|
H
|
|
820 |
|
|
|
|
|
|
|
|
|
|
V
|
I
|
F
|
A
|
L
|
L
|
N
|
A
|
F
|
Q
|
830 |
|
|
|
|
|
|
|
|
|
|
G
|
F
|
F
|
I
|
L
|
C
|
F
|
G
|
I
|
L
|
840 |
|
|
|
|
|
|
|
|
|
|
H8 | |||||||||
L
|
D
|
S
|
K
|
L
|
R
|
Q
|
L
|
L
|
F
|
850 |
|
|
|
|
|
|
|
|
|
|
N
|
K
|
L
|
S
|
A
|
L
|
S
|
S
|
W
|
K
|
860 |
|
|
|
|
|
|
|
|
|
|
C-term | |||||||||
Q
|
T
|
E
|
K
|
Q
|
N
|
S
|
S
|
D
|
L
|
870 |
|
|
|
|
|
|
|
|
|
|
S
|
A
|
K
|
P
|
K
|
F
|
S
|
K
|
P
|
F
|
880 |
|
|
|
|
|
|
|
|
|
|
N
|
P
|
L
|
Q
|
N
|
K
|
G
|
H
|
Y
|
A
|
890 |
|
|
|
|
|
|
|
|
|
|
F
|
S
|
H
|
T
|
G
|
D
|
S
|
S
|
D
|
N
|
900 |
|
|
|
|
|
|
|
|
|
|
I
|
M
|
L
|
T
|
Q
|
F
|
V
|
S
|
N
|
E
|
910 |
N-term | |||||||||
M
|
K
|
V
|
G
|
V
|
L
|
W
|
L
|
I
|
S
|
10 |
F
|
F
|
T
|
F
|
T
|
D
|
G
|
H
|
G
|
G
|
20 |
F
|
L
|
G
|
K
|
N
|
D
|
G
|
I
|
K
|
T
|
30 |
K
|
K
|
E
|
L
|
I
|
V
|
N
|
K
|
K
|
K
|
40 |
H
|
L
|
G
|
P
|
V
|
E
|
E
|
Y
|
Q
|
L
|
50 |
L
|
L
|
Q
|
V
|
T
|
Y
|
R
|
D
|
S
|
K
|
60 |
E
|
K
|
R
|
D
|
L
|
R
|
N
|
F
|
L
|
K
|
70 |
L
|
L
|
K
|
P
|
P
|
L
|
L
|
W
|
S
|
H
|
80 |
G
|
L
|
I
|
R
|
I
|
I
|
R
|
A
|
K
|
A
|
90 |
T
|
T
|
D
|
C
|
N
|
S
|
L
|
N
|
G
|
V
|
100 |
L
|
Q
|
C
|
T
|
C
|
E
|
D
|
S
|
Y
|
T
|
110 |
W
|
F
|
P
|
P
|
S
|
C
|
L
|
D
|
P
|
Q
|
120 |
N
|
C
|
Y
|
L
|
H
|
T
|
A
|
G
|
A
|
L
|
130 |
P
|
S
|
C
|
E
|
C
|
H
|
L
|
N
|
N
|
L
|
140 |
S
|
Q
|
S
|
V
|
N
|
F
|
C
|
E
|
R
|
T
|
150 |
K
|
I
|
W
|
G
|
T
|
F
|
K
|
I
|
N
|
E
|
160 |
R
|
F
|
T
|
N
|
D
|
L
|
L
|
N
|
S
|
S
|
170 |
S
|
A
|
I
|
Y
|
S
|
K
|
Y
|
A
|
N
|
G
|
180 |
I
|
E
|
I
|
Q
|
L
|
K
|
K
|
A
|
Y
|
E
|
190 |
R
|
I
|
Q
|
G
|
F
|
E
|
S
|
V
|
Q
|
V
|
200 |
T
|
Q
|
F
|
R
|
N
|
G
|
S
|
I
|
V
|
A
|
210 |
G
|
Y
|
E
|
V
|
V
|
G
|
S
|
S
|
S
|
A
|
220 |
S
|
E
|
L
|
L
|
S
|
A
|
I
|
E
|
H
|
V
|
230 |
A
|
E
|
K
|
A
|
K
|
T
|
A
|
L
|
H
|
K
|
240 |
L
|
F
|
P
|
L
|
E
|
D
|
G
|
S
|
F
|
R
|
250 |
V
|
F
|
G
|
K
|
A
|
Q
|
C
|
N
|
D
|
I
|
260 |
V
|
F
|
G
|
F
|
G
|
S
|
K
|
D
|
D
|
E
|
270 |
Y
|
T
|
L
|
P
|
C
|
S
|
S
|
G
|
Y
|
R
|
280 |
G
|
N
|
I
|
T
|
A
|
K
|
C
|
E
|
S
|
S
|
290 |
G
|
W
|
Q
|
V
|
I
|
R
|
E
|
T
|
C
|
V
|
300 |
L
|
S
|
L
|
L
|
E
|
E
|
L
|
N
|
K
|
N
|
310 |
F
|
S
|
M
|
I
|
V
|
G
|
N
|
A
|
T
|
E
|
320 |
A
|
A
|
V
|
S
|
S
|
F
|
V
|
Q
|
N
|
L
|
330 |
S
|
V
|
I
|
I
|
R
|
Q
|
N
|
P
|
S
|
T
|
340 |
T
|
V
|
G
|
N
|
L
|
A
|
S
|
V
|
V
|
S
|
350 |
I
|
L
|
S
|
N
|
I
|
S
|
S
|
L
|
S
|
L
|
360 |
A
|
S
|
H
|
F
|
R
|
V
|
S
|
N
|
S
|
T
|
370 |
M
|
E
|
D
|
V
|
I
|
S
|
I
|
A
|
D
|
N
|
380 |
I
|
L
|
N
|
S
|
A
|
S
|
V
|
T
|
N
|
W
|
390 |
|
||||
T
|
V
|
L
|
L
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|