pe2r4_human
NAME
8GDA
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
pe2r4_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
N-term | |||||||||
M
|
S
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T
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P
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G
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V
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N
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S
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S
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A
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10 |
TM1 | |||||||||
S
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L
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S
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P
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D
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R
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L
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N
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S
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P
|
20 |
V
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T
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I
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P
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A
|
V
|
M
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F
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I
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F
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30 |
G
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V
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V
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G
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N
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L
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V
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A
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I
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V
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40 |
ICL1 | |||||||||
V
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L
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C
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K
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S
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R
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K
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E
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Q
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K
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50 |
TM2 | |||||||||
E
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T
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T
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F
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Y
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T
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L
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V
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C
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G
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60 |
L
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A
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V
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T
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D
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L
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L
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G
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T
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L
|
70 |
L
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V
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S
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P
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V
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T
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I
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A
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T
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Y
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80 |
ECL1 | TM3 | ||||||||
M
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K
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G
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Q
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W
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P
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G
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G
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Q
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P
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90 |
L
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C
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E
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Y
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S
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T
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F
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I
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L
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L
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100 |
F
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F
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S
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L
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S
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G
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L
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S
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I
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I
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110 |
C
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A
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M
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S
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V
|
E
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R
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Y
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L
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A
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120 |
ICL2 | |||||||||
I
|
N
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H
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A
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Y
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F
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Y
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S
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H
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Y
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130 |
TM4 | |||||||||
V
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D
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K
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R
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L
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A
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G
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L
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T
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L
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140 |
F
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A
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V
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Y
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A
|
S
|
N
|
V
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L
|
F
|
150 |
C
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A
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L
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P
|
N
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M
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G
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L
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G
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S
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160 |
ECL2 | |||||||||
S
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R
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L
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Q
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Y
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P
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D
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T
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W
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C
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170 |
TM5 | |||||||||
F
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I
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D
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W
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T
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T
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N
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V
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T
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A
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180 |
H
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A
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A
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Y
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S
|
Y
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M
|
Y
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A
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G
|
190 |
F
|
S
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S
|
F
|
L
|
I
|
L
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A
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T
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V
|
200 |
L
|
C
|
N
|
V
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L
|
V
|
C
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G
|
A
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L
|
210 |
L
|
R
|
M
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H
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R
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Q
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F
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M
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R
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R
|
220 |
ICL3 | |||||||||
T
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S
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L
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G
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T
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E
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Q
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H
|
H
|
A
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230 |
A
|
A
|
A
|
A
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S
|
V
|
A
|
S
|
R
|
G
|
240 |
H
|
P
|
A
|
A
|
S
|
P
|
A
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L
|
P
|
R
|
250 |
L
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S
|
D
|
F
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R
|
R
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R
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R
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S
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F
|
260 |
TM6 | |||||||||
R
|
R
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I
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A
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G
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A
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E
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I
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Q
|
M
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270 |
V
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I
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L
|
L
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I
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A
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T
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S
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L
|
V
|
280 |
V
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L
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I
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C
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S
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I
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P
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L
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V
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V
|
290 |
R
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V
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F
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V
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N
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Q
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L
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Y
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Q
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P
|
300 |
ECL3 | |||||||||
S
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L
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E
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R
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E
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V
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S
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K
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N
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P
|
310 |
TM7 | |||||||||
D
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L
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Q
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A
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I
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R
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I
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A
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S
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V
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320 |
N
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P
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I
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L
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D
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P
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W
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I
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Y
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I
|
330 |
H8 | |||||||||
L
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L
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R
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K
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T
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V
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L
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S
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K
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A
|
340 |
I
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E
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K
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I
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K
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C
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L
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F
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C
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R
|
350 |
C-term | |||||||||
I
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G
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G
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S
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R
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R
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E
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R
|
S
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G
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360 |
Q
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H
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C
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S
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D
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S
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Q
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R
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T
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S
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370 |
S
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A
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M
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S
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G
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H
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S
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R
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S
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F
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380 |
I
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S
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R
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E
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L
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K
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E
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I
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S
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S
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390 |
T
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S
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Q
|
T
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L
|
L
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P
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D
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L
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S
|
400 |
L
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P
|
D
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L
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S
|
E
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N
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G
|
L
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G
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410 |
G
|
R
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N
|
L
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L
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P
|
G
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V
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P
|
G
|
420 |
M
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G
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L
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A
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Q
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E
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D
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T
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T
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S
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430 |
L
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R
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T
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L
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R
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I
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S
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E
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T
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S
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440 |
D
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S
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S
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Q
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G
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Q
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D
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S
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E
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S
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450 |
V
|
L
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L
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V
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D
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E
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A
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G
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G
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S
|
460 |
G
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R
|
A
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G
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P
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A
|
P
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K
|
G
|
S
|
470 |
S
|
L
|
Q
|
V
|
T
|
F
|
P
|
S
|
E
|
T
|
480 |
L
|
N
|
L
|
S
|
E
|
K
|
C
|
I
|
N-term | |||||||||
M
|
S
|
T
|
P
|
G
|
V
|
N
|
S
|
S
|
A
|
10 |
TM1 | |||||||||
S
|
L
|
S
|
P
|
D
|
R
|
L
|
N
|
S
|
P
|
20 |
V
|
T
|
I
|
P
|
A
|
V
|
M
|
F
|
I
|
F
|
30 |
G
|
V
|
V
|
G
|
N
|
L
|
V
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A
|
I
|
V
|
40 |
ICL1 | |||||||||
V
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L
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C
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K
|
S
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R
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K
|
E
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Q
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K
|
50 |
TM2 | |||||||||
E
|
T
|
T
|
F
|
Y
|
T
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L
|
V
|
C
|
G
|
60 |
L
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A
|
V
|
T
|
D
|
L
|
L
|
G
|
T
|
L
|
70 |
L
|
V
|
S
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P
|
V
|
T
|
I
|
A
|
T
|
Y
|
80 |
ECL1 | TM3 | ||||||||
M
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K
|
G
|
Q
|
W
|
P
|
G
|
G
|
Q
|
P
|
90 |
L
|
C
|
E
|
Y
|
S
|
T
|
F
|
I
|
L
|
L
|
100 |
F
|
F
|
S
|
L
|
S
|
G
|
L
|
S
|
I
|
I
|
110 |
C
|
A
|
M
|
S
|
V
|
E
|
R
|
Y
|
L
|
A
|
120 |
ICL2 | |||||||||
I
|
N
|
H
|
A
|
Y
|
F
|
Y
|
S
|
H
|
Y
|
130 |
TM4 | |||||||||
V
|
D
|
K
|
R
|
L
|
A
|
G
|
L
|
T
|
L
|
140 |
F
|
A
|
V
|
Y
|
A
|
S
|
N
|
V
|
L
|
F
|
150 |
C
|
A
|
L
|
P
|
N
|
M
|
G
|
L
|
G
|
S
|
160 |
ECL2 | |||||||||
S
|
R
|
L
|
Q
|
Y
|
P
|
D
|
T
|
W
|
C
|
170 |
TM5 | |||||||||
F
|
I
|
D
|
W
|
T
|
T
|
N
|
V
|
T
|
A
|
180 |
H
|
A
|
A
|
Y
|
S
|
Y
|
M
|
Y
|
A
|
G
|
190 |
F
|
S
|
S
|
F
|
L
|
I
|
L
|
A
|
T
|
V
|
200 |
L
|
C
|
N
|
V
|
L
|
V
|
C
|
G
|
A
|
L
|
210 |
L
|
R
|
M
|
H
|
R
|
Q
|
F
|
M
|
R
|
R
|
220 |
ICL3 | |||||||||
T
|
S
|
L
|
G
|
T
|
E
|
Q
|
H
|
H
|
A
|
230 |
A
|
A
|
A
|
A
|
S
|
V
|
A
|
S
|
R
|
G
|
240 |
H
|
P
|
A
|
A
|
S
|
P
|
A
|
L
|
P
|
R
|
250 |
L
|
S
|
D
|
F
|
R
|
R
|
R
|
R
|
S
|
F
|
260 |
TM6 | |||||||||
R
|
R
|
I
|
A
|
G
|
A
|
E
|
I
|
Q
|
M
|
270 |
V
|
I
|
L
|
L
|
I
|
A
|
T
|
S
|
L
|
V
|
280 |
V
|
L
|
I
|
C
|
S
|
I
|
P
|
L
|
V
|
V
|
290 |
R
|
V
|
F
|
V
|
N
|
Q
|
L
|
Y
|
Q
|
P
|
300 |
ECL3 | |||||||||
S
|
L
|
E
|
R
|
E
|
V
|
S
|
K
|
N
|
P
|
310 |
TM7 | |||||||||
D
|
L
|
Q
|
A
|
I
|
R
|
I
|
A
|
S
|
V
|
320 |
N
|
P
|
I
|
L
|
D
|
P
|
W
|
I
|
Y
|
I
|
330 |
H8 | |||||||||
L
|
L
|
R
|
K
|
T
|
V
|
L
|
S
|
K
|
A
|
340 |
I
|
E
|
K
|
I
|
K
|
C
|
L
|
F
|
C
|
R
|
350 |
C-term | |||||||||
I
|
G
|
G
|
S
|
R
|
R
|
E
|
R
|
S
|
G
|
360 |
Q
|
H
|
C
|
S
|
D
|
S
|
Q
|
R
|
T
|
S
|
370 |
S
|
A
|
M
|
S
|
G
|
H
|
S
|
R
|
S
|
F
|
380 |
I
|
S
|
R
|
E
|
L
|
K
|
E
|
I
|
S
|
S
|
390 |
T
|
S
|
Q
|
T
|
L
|
L
|
P
|
D
|
L
|
S
|
400 |
L
|
P
|
D
|
L
|
S
|
E
|
N
|
G
|
L
|
G
|
410 |
G
|
R
|
N
|
L
|
L
|
P
|
G
|
V
|
P
|
G
|
420 |
M
|
G
|
L
|
A
|
Q
|
E
|
D
|
T
|
T
|
S
|
430 |
L
|
R
|
T
|
L
|
R
|
I
|
S
|
E
|
T
|
S
|
440 |
D
|
S
|
S
|
Q
|
G
|
Q
|
D
|
S
|
E
|
S
|
450 |
V
|
L
|
L
|
V
|
D
|
E
|
A
|
G
|
G
|
S
|
460 |
G
|
R
|
A
|
G
|
P
|
A
|
P
|
K
|
G
|
S
|
470 |
S
|
L
|
Q
|
V
|
T
|
F
|
P
|
S
|
E
|
T
|
480 |
L
|
N
|
L
|
S
|
E
|
K
|
C
|
I
|
488 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|