grm4_human
NAME
8JD6
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
grm4_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
|
P
|
G
|
K
|
R
|
G
|
L
|
G
|
W
|
W
|
10 |
|
|
|
|
|
|
|
|
|
|
W
|
A
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R
|
L
|
P
|
L
|
C
|
L
|
L
|
L
|
20 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
Y
|
G
|
P
|
W
|
M
|
P
|
S
|
S
|
30 |
|
|
||||||||
L
|
G
|
K
|
P
|
K
|
G
|
H
|
P
|
H
|
M
|
40 |
N
|
S
|
I
|
R
|
I
|
D
|
G
|
D
|
I
|
T
|
50 |
L
|
G
|
G
|
L
|
F
|
P
|
V
|
H
|
G
|
R
|
60 |
G
|
S
|
E
|
G
|
K
|
P
|
C
|
G
|
E
|
L
|
70 |
K
|
K
|
E
|
K
|
G
|
I
|
H
|
R
|
L
|
E
|
80 |
A
|
M
|
L
|
F
|
A
|
L
|
D
|
R
|
I
|
N
|
90 |
N
|
D
|
P
|
D
|
L
|
L
|
P
|
N
|
I
|
T
|
100 |
L
|
G
|
A
|
R
|
I
|
L
|
D
|
T
|
C
|
S
|
110 |
R
|
D
|
T
|
H
|
A
|
L
|
E
|
Q
|
S
|
L
|
120 |
T
|
F
|
V
|
Q
|
A
|
L
|
I
|
E
|
K
|
D
|
130 |
G
|
T
|
E
|
V
|
R
|
C
|
G
|
S
|
G
|
G
|
140 |
P
|
P
|
I
|
I
|
T
|
K
|
P
|
E
|
R
|
V
|
150 |
V
|
G
|
V
|
I
|
G
|
A
|
S
|
G
|
S
|
S
|
160 |
V
|
S
|
I
|
M
|
V
|
A
|
N
|
I
|
L
|
R
|
170 |
L
|
F
|
K
|
I
|
P
|
Q
|
I
|
S
|
Y
|
A
|
180 |
S
|
T
|
A
|
P
|
D
|
L
|
S
|
D
|
N
|
S
|
190 |
R
|
Y
|
D
|
F
|
F
|
S
|
R
|
V
|
V
|
P
|
200 |
S
|
D
|
T
|
Y
|
Q
|
A
|
Q
|
A
|
M
|
V
|
210 |
D
|
I
|
V
|
R
|
A
|
L
|
K
|
W
|
N
|
Y
|
220 |
V
|
S
|
T
|
V
|
A
|
S
|
E
|
G
|
S
|
Y
|
230 |
G
|
E
|
S
|
G
|
V
|
E
|
A
|
F
|
I
|
Q
|
240 |
K
|
S
|
R
|
E
|
D
|
G
|
G
|
V
|
C
|
I
|
250 |
A
|
Q
|
S
|
V
|
K
|
I
|
P
|
R
|
E
|
P
|
260 |
K
|
A
|
G
|
E
|
F
|
D
|
K
|
I
|
I
|
R
|
270 |
R
|
L
|
L
|
E
|
T
|
S
|
N
|
A
|
R
|
A
|
280 |
V
|
I
|
I
|
F
|
A
|
N
|
E
|
D
|
D
|
I
|
290 |
R
|
R
|
V
|
L
|
E
|
A
|
A
|
R
|
R
|
A
|
300 |
N
|
Q
|
T
|
G
|
H
|
F
|
F
|
W
|
M
|
G
|
310 |
S
|
D
|
S
|
W
|
G
|
S
|
K
|
I
|
A
|
P
|
320 |
V
|
L
|
H
|
L
|
E
|
E
|
V
|
A
|
E
|
G
|
330 |
A
|
V
|
T
|
I
|
L
|
P
|
K
|
R
|
M
|
S
|
340 |
V
|
R
|
G
|
F
|
D
|
R
|
Y
|
F
|
S
|
S
|
350 |
R
|
T
|
L
|
D
|
N
|
N
|
R
|
R
|
N
|
I
|
360 |
W
|
F
|
A
|
E
|
F
|
W
|
E
|
D
|
N
|
F
|
370 |
H
|
C
|
K
|
L
|
S
|
R
|
H
|
A
|
L
|
K
|
380 |
K
|
G
|
S
|
H
|
V
|
K
|
K
|
C
|
T
|
N
|
390 |
R
|
E
|
R
|
I
|
G
|
Q
|
D
|
S
|
A
|
Y
|
400 |
E
|
Q
|
E
|
G
|
K
|
V
|
Q
|
F
|
V
|
I
|
410 |
D
|
A
|
V
|
Y
|
A
|
M
|
G
|
H
|
A
|
L
|
420 |
H
|
A
|
M
|
H
|
R
|
D
|
L
|
C
|
P
|
G
|
430 |
R
|
V
|
G
|
L
|
C
|
P
|
R
|
M
|
D
|
P
|
440 |
V
|
D
|
G
|
T
|
Q
|
L
|
L
|
K
|
Y
|
I
|
450 |
R
|
N
|
V
|
N
|
F
|
S
|
G
|
I
|
A
|
G
|
460 |
N
|
P
|
V
|
T
|
F
|
N
|
E
|
N
|
G
|
D
|
470 |
A
|
P
|
G
|
R
|
Y
|
D
|
I
|
Y
|
Q
|
Y
|
480 |
Q
|
L
|
R
|
N
|
D
|
S
|
A
|
E
|
Y
|
K
|
490 |
V
|
I
|
G
|
S
|
W
|
T
|
D
|
H
|
L
|
H
|
500 |
L
|
R
|
I
|
E
|
R
|
M
|
H
|
W
|
P
|
G
|
510 |
S
|
G
|
Q
|
Q
|
L
|
P
|
R
|
S
|
I
|
C
|
520 |
S
|
L
|
P
|
C
|
Q
|
P
|
G
|
E
|
R
|
K
|
530 |
K
|
T
|
V
|
K
|
G
|
M
|
P
|
C
|
C
|
W
|
540 |
H
|
C
|
E
|
P
|
C
|
T
|
G
|
Y
|
Q
|
Y
|
550 |
Q
|
V
|
D
|
R
|
Y
|
T
|
C
|
K
|
T
|
C
|
560 |
P
|
Y
|
D
|
M
|
R
|
P
|
T
|
E
|
N
|
R
|
570 |
T
|
G
|
C
|
R
|
P
|
I
|
P
|
I
|
I
|
K
|
580 |
TM1 | |||||||||
L
|
E
|
W
|
G
|
S
|
P
|
W
|
A
|
V
|
L
|
590 |
P
|
L
|
F
|
L
|
A
|
V
|
V
|
G
|
I
|
A
|
600 |
A
|
T
|
L
|
F
|
V
|
V
|
I
|
T
|
F
|
V
|
610 |
ICL1 | |||||||||
R
|
Y
|
N
|
D
|
T
|
P
|
I
|
V
|
K
|
A
|
620 |
TM2 | |||||||||
S
|
G
|
R
|
E
|
L
|
S
|
Y
|
V
|
L
|
L
|
630 |
A
|
G
|
I
|
F
|
L
|
C
|
Y
|
A
|
T
|
T
|
640 |
ECL1 | TM3 | |||||||||
F
|
L
|
M
|
I
|
A
|
E
|
P
|
D
|
L
|
G
|
|
650 |
T
|
C
|
S
|
L
|
R
|
R
|
I
|
F
|
L
|
G
|
660 |
L
|
G
|
M
|
S
|
I
|
S
|
Y
|
A
|
A
|
L
|
670 |
L
|
T
|
K
|
T
|
N
|
R
|
I
|
Y
|
R
|
I
|
680 |
F
|
E
|
Q
|
G
|
K
|
R
|
S
|
V
|
S
|
A
|
690 |
ICL2 | TM4 | ||||||||
P
|
R
|
F
|
I
|
S
|
P
|
A
|
S
|
Q
|
L
|
700 |
A
|
I
|
T
|
F
|
S
|
L
|
I
|
S
|
L
|
Q
|
710 |
L
|
L
|
G
|
I
|
C
|
V
|
W
|
F
|
V
|
V
|
720 |
ECL2 | |||||||||
D
|
P
|
S
|
H
|
S
|
V
|
V
|
D
|
F
|
Q
|
730 |
D
|
Q
|
R
|
T
|
L
|
D
|
P
|
R
|
F
|
A
|
740 |
R
|
G
|
V
|
L
|
K
|
C
|
D
|
I
|
S
|
D
|
750 |
TM5 | |||||||||
L
|
S
|
L
|
I
|
C
|
L
|
L
|
G
|
Y
|
S
|
760 |
M
|
L
|
L
|
M
|
V
|
T
|
C
|
T
|
V
|
Y
|
770 |
ICL3 | |||||||||
A
|
I
|
K
|
T
|
R
|
G
|
V
|
P
|
E
|
T
|
780 |
TM6 | |||||||||
F
|
N
|
E
|
A
|
K
|
P
|
I
|
G
|
F
|
T
|
790 |
M
|
Y
|
T
|
T
|
C
|
I
|
V
|
W
|
L
|
A
|
800 |
F
|
I
|
P
|
I
|
F
|
F
|
G
|
T
|
S
|
Q
|
810 |
ECL3 | TM7 | ||||||||
S
|
A
|
D
|
K
|
L
|
Y
|
I
|
Q
|
T
|
T
|
820 |
T
|
L
|
T
|
V
|
S
|
V
|
S
|
L
|
S
|
A
|
830 |
S
|
V
|
S
|
L
|
G
|
M
|
L
|
Y
|
M
|
P
|
840 |
H8 | |||||||||
K
|
V
|
Y
|
I
|
I
|
L
|
F
|
H
|
P
|
E
|
850 |
Q
|
N
|
V
|
P
|
K
|
R
|
K
|
R
|
S
|
L
|
860 |
C-term | |||||||||
K
|
A
|
V
|
V
|
T
|
A
|
A
|
T
|
M
|
S
|
870 |
N
|
K
|
F
|
T
|
Q
|
K
|
G
|
N
|
F
|
R
|
880 |
P
|
N
|
G
|
E
|
A
|
K
|
S
|
E
|
L
|
C
|
890 |
E
|
N
|
L
|
E
|
A
|
P
|
A
|
L
|
A
|
T
|
900 |
K
|
Q
|
T
|
Y
|
V
|
T
|
Y
|
T
|
N
|
H
|
910 |
A
|
I
|
|
|||||||||
N-term | |||||||||
|
K
|
P
|
K
|
G
|
H
|
P
|
H
|
M
|
N
|
41 |
S
|
I
|
R
|
I
|
D
|
G
|
D
|
I
|
T
|
L
|
51 |
G
|
G
|
L
|
F
|
P
|
V
|
H
|
G
|
R
|
G
|
61 |
S
|
E
|
G
|
K
|
P
|
C
|
G
|
E
|
L
|
K
|
71 |
K
|
E
|
K
|
G
|
I
|
H
|
R
|
L
|
E
|
A
|
81 |
M
|
L
|
F
|
A
|
L
|
D
|
R
|
I
|
N
|
N
|
91 |
D
|
P
|
D
|
L
|
L
|
P
|
N
|
I
|
T
|
L
|
101 |
G
|
A
|
R
|
I
|
L
|
D
|
T
|
C
|
S
|
R
|
111 |
D
|
T
|
H
|
A
|
L
|
E
|
Q
|
S
|
L
|
T
|
121 |
F
|
V
|
Q
|
A
|
L
|
I
|
E
|
K
|
D
|
G
|
131 |
T
|
E
|
V
|
R
|
C
|
G
|
S
|
G
|
G
|
P
|
141 |
P
|
I
|
I
|
T
|
K
|
P
|
E
|
R
|
V
|
V
|
151 |
G
|
V
|
I
|
G
|
A
|
S
|
G
|
S
|
S
|
V
|
161 |
S
|
I
|
M
|
V
|
A
|
N
|
I
|
L
|
R
|
L
|
171 |
F
|
K
|
I
|
P
|
Q
|
I
|
S
|
Y
|
A
|
S
|
181 |
T
|
A
|
P
|
D
|
L
|
S
|
D
|
N
|
S
|
R
|
191 |
Y
|
D
|
F
|
F
|
S
|
R
|
V
|
V
|
P
|
S
|
201 |
D
|
T
|
Y
|
Q
|
A
|
Q
|
A
|
M
|
V
|
D
|
211 |
I
|
V
|
R
|
A
|
L
|
K
|
W
|
N
|
Y
|
V
|
221 |
S
|
T
|
V
|
A
|
S
|
E
|
G
|
S
|
Y
|
G
|
231 |
E
|
S
|
G
|
V
|
E
|
A
|
F
|
I
|
Q
|
K
|
241 |
S
|
R
|
E
|
D
|
G
|
G
|
V
|
C
|
I
|
A
|
251 |
Q
|
S
|
V
|
K
|
I
|
P
|
R
|
E
|
P
|
K
|
261 |
A
|
G
|
E
|
F
|
D
|
K
|
I
|
I
|
R
|
R
|
271 |
L
|
L
|
E
|
T
|
S
|
N
|
A
|
R
|
A
|
V
|
281 |
I
|
I
|
F
|
A
|
N
|
E
|
D
|
D
|
I
|
R
|
291 |
R
|
V
|
L
|
E
|
A
|
A
|
R
|
R
|
A
|
N
|
301 |
Q
|
T
|
G
|
H
|
F
|
F
|
W
|
M
|
G
|
S
|
311 |
D
|
S
|
W
|
G
|
S
|
K
|
I
|
A
|
P
|
V
|
321 |
L
|
H
|
L
|
E
|
E
|
V
|
A
|
E
|
G
|
A
|
331 |
V
|
T
|
I
|
L
|
P
|
K
|
R
|
M
|
S
|
V
|
341 |
R
|
G
|
F
|
D
|
R
|
Y
|
F
|
S
|
S
|
R
|
351 |
T
|
L
|
D
|
N
|
N
|
R
|
R
|
N
|
I
|
W
|
361 |
F
|
A
|
E
|
F
|
W
|
E
|
D
|
N
|
F
|
H
|
371 |
C
|
K
|
L
|
S
|
R
|
H
|
A
|
L
|
K
|
K
|
381 |
G
|
S
|
H
|
V
|
K
|
K
|
C
|
T
|
N
|
R
|
391 |
E
|
R
|
I
|
G
|
Q
|
D
|
S
|
A
|
Y
|
E
|
401 |
Q
|
E
|
G
|
K
|
V
|
Q
|
F
|
V
|
I
|
D
|
411 |
A
|
V
|
Y
|
A
|
M
|
G
|
H
|
A
|
L
|
H
|
421 |
A
|
M
|
H
|
R
|
D
|
L
|
C
|
P
|
G
|
R
|
431 |
V
|
G
|
L
|
C
|
P
|
R
|
M
|
D
|
P
|
V
|
441 |
D
|
G
|
T
|
Q
|
L
|
L
|
K
|
Y
|
I
|
R
|
451 |
N
|
V
|
N
|
F
|
S
|
G
|
I
|
A
|
G
|
N
|
461 |
P
|
V
|
T
|
F
|
N
|
E
|
N
|
G
|
D
|
A
|
471 |
P
|
G
|
R
|
Y
|
D
|
I
|
Y
|
Q
|
Y
|
Q
|
481 |
L
|
R
|
N
|
D
|
S
|
A
|
E
|
Y
|
K
|
V
|
491 |
I
|
G
|
S
|
W
|
T
|
D
|
H
|
L
|
H
|
L
|
501 |
R
|
I
|
E
|
R
|
M
|
H
|
W
|
P
|
G
|
S
|
511 |
G
|
Q
|
Q
|
L
|
P
|
R
|
S
|
I
|
C
|
S
|
521 |
L
|
P
|
C
|
Q
|
P
|
G
|
E
|
R
|
K
|
K
|
531 |
T
|
V
|
K
|
G
|
M
|
P
|
C
|
C
|
W
|
H
|
541 |
C
|
E
|
P
|
C
|
T
|
G
|
Y
|
Q
|
Y
|
Q
|
551 |
V
|
D
|
R
|
Y
|
T
|
C
|
K
|
T
|
C
|
P
|
561 |
Y
|
D
|
M
|
R
|
P
|
T
|
E
|
N
|
R
|
T
|
571 |
G
|
C
|
R
|
P
|
I
|
P
|
I
|
I
|
K
|
L
|
581 |
TM1 | |||||||||
E
|
W
|
G
|
S
|
P
|
W
|
A
|
V
|
L
|
P
|
591 |
L
|
F
|
L
|
A
|
V
|
V
|
G
|
I
|
A
|
A
|
601 |
T
|
L
|
F
|
V
|
V
|
I
|
T
|
F
|
V
|
R
|
611 |
ICL1 | |||||||||
Y
|
N
|
D
|
T
|
P
|
I
|
V
|
K
|
A
|
S
|
621 |
TM2 | |||||||||
G
|
R
|
E
|
L
|
S
|
Y
|
V
|
L
|
L
|
A
|
631 |
G
|
I
|
F
|
L
|
C
|
Y
|
A
|
T
|
T
|
F
|
641 |
ECL1 | TM3 | ||||||||
L
|
M
|
I
|
A
|
E
|
P
|
D
|
L
|
G
|
T
|
651 |
C
|
S
|
L
|
R
|
R
|
I
|
F
|
L
|
G
|
L
|
661 |
G
|
M
|
S
|
I
|
S
|
Y
|
A
|
A
|
L
|
L
|
671 |
T
|
K
|
T
|
N
|
R
|
I
|
Y
|
R
|
I
|
F
|
681 |
ICL2 | |||||||||
E
|
Q
|
G
|
K
|
R
|
S
|
V
|
S
|
A
|
P
|
691 |
TM4 | |||||||||
R
|
F
|
I
|
S
|
P
|
A
|
S
|
Q
|
L
|
A
|
701 |
I
|
T
|
F
|
S
|
L
|
I
|
S
|
L
|
Q
|
L
|
711 |
L
|
G
|
I
|
C
|
V
|
W
|
F
|
V
|
V
|
D
|
721 |
ECL2 | |||||||||
P
|
S
|
H
|
S
|
V
|
V
|
D
|
F
|
Q
|
D
|
731 |
Q
|
R
|
T
|
L
|
D
|
P
|
R
|
F
|
A
|
R
|
741 |
TM5 | |||||||||
G
|
V
|
L
|
K
|
C
|
D
|
I
|
S
|
D
|
L
|
751 |
S
|
L
|
I
|
C
|
L
|
L
|
G
|
Y
|
S
|
M
|
761 |
L
|
L
|
M
|
V
|
T
|
C
|
T
|
V
|
Y
|
A
|
771 |
ICL3 | |||||||||
I
|
K
|
T
|
R
|
G
|
V
|
P
|
E
|
T
|
F
|
781 |
TM6 | |||||||||
N
|
E
|
A
|
K
|
P
|
I
|
G
|
F
|
T
|
M
|
791 |
Y
|
T
|
T
|
C
|
I
|
V
|
W
|
L
|
A
|
F
|
801 |
I
|
P
|
I
|
F
|
F
|
G
|
T
|
S
|
Q
|
S
|
811 |
ECL3 | TM7 | ||||||||
A
|
D
|
K
|
L
|
Y
|
I
|
Q
|
T
|
T
|
T
|
821 |
L
|
T
|
V
|
S
|
V
|
S
|
L
|
S
|
A
|
S
|
831 |
V
|
S
|
L
|
G
|
M
|
L
|
Y
|
M
|
P
|
K
|
841 |
H8 | |||||||||
V
|
Y
|
I
|
I
|
L
|
F
|
H
|
P
|
E
|
Q
|
851 |
N
|
V
|
P
|
K
|
R
|
K
|
R
|
S
|
L
|
K
|
861 |
C-term | |||||||||
A
|
V
|
V
|
T
|
A
|
A
|
T
|
M
|
S
|
N
|
871 |
K
|
F
|
T
|
Q
|
K
|
G
|
N
|
F
|
R
|
P
|
881 |
N
|
G
|
E
|
A
|
K
|
S
|
E
|
L
|
C
|
E
|
891 |
N
|
L
|
E
|
A
|
P
|
A
|
L
|
A
|
T
|
K
|
901 |
Q
|
T
|
Y
|
V
|
T
|
Y
|
T
|
N
|
H
|
A
|
911 |
I
|
912 |
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|