gpr34_human
NAME
8SAI
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
gpr34_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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R
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S
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H
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T
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I
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T
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M
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T
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T
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10 |
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|
|
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T
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S
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V
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S
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S
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W
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P
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Y
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S
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S
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20 |
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|
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|
|
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H
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R
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M
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R
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F
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I
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T
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N
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H
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S
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30 |
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|
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|
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|||
D
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Q
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P
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P
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Q
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N
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F
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S
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A
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T
|
40 |
P
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N
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V
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T
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T
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C
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P
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M
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D
|
E
|
50 |
TM1 | |||||||||
K
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L
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L
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S
|
T
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V
|
L
|
T
|
T
|
S
|
60 |
Y
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S
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V
|
I
|
F
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I
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V
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G
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L
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V
|
70 |
G
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N
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I
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I
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A
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L
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Y
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V
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F
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L
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80 |
ICL1 | TM2 | ||||||||
G
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I
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H
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R
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K
|
R
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N
|
S
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I
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Q
|
90 |
I
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Y
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L
|
L
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N
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V
|
A
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I
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A
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D
|
100 |
L
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L
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L
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I
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F
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C
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L
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P
|
F
|
R
|
110 |
I
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M
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Y
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H
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I
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N
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Q
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N
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K
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W
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120 |
ECL1 | TM3 | ||||||||
T
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L
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G
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V
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I
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L
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C
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K
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V
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V
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130 |
G
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T
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L
|
F
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Y
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M
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N
|
M
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Y
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I
|
140 |
S
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I
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I
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L
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L
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G
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F
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I
|
S
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L
|
150 |
D
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R
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Y
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I
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K
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I
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N
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R
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S
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I
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160 |
ICL2 | |||||||||
Q
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Q
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R
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K
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A
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I
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T
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T
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K
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Q
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170 |
TM4 | |||||||||
S
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I
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Y
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V
|
C
|
C
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I
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V
|
W
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M
|
180 |
L
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A
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L
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G
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G
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F
|
L
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T
|
M
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I
|
190 |
I
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L
|
T
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L
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K
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K
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G
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G
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H
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N
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200 |
ECL2 | |||||||||
S
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T
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M
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C
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F
|
H
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Y
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R
|
D
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K
|
210 |
TM5 | |||||||||
H
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N
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A
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K
|
G
|
E
|
A
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I
|
F
|
N
|
220 |
F
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I
|
L
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V
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V
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M
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F
|
W
|
L
|
I
|
230 |
F
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L
|
L
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I
|
I
|
L
|
S
|
Y
|
I
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K
|
240 |
I
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G
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K
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N
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L
|
L
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R
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I
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S
|
K
|
250 |
ICL3 | TM6 | ||||||||
R
|
R
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S
|
K
|
F
|
P
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N
|
S
|
G
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K
|
260 |
Y
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A
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T
|
T
|
A
|
R
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N
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S
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F
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I
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270 |
V
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L
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I
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I
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F
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T
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I
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C
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F
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V
|
280 |
P
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Y
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H
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A
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F
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R
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F
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I
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Y
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I
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290 |
ECL3 | TM7 | ||||||||
S
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S
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Q
|
L
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N
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V
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S
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S
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C
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Y
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300 |
W
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K
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E
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I
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V
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H
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K
|
T
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N
|
E
|
310 |
I
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M
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L
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V
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L
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S
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S
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F
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N
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S
|
320 |
C
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L
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D
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P
|
V
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M
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Y
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F
|
L
|
M
|
330 |
H8 | |||||||||
S
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S
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N
|
I
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R
|
K
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I
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M
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C
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Q
|
340 |
L
|
L
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F
|
R
|
R
|
F
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Q
|
G
|
E
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P
|
350 |
C-term | |||||||||
S
|
R
|
S
|
E
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S
|
T
|
S
|
E
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F
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K
|
360 |
P
|
G
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Y
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S
|
L
|
H
|
D
|
T
|
S
|
V
|
370 |
|
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|
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|
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|||
A
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V
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K
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I
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Q
|
S
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S
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S
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K
|
S
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380 |
|
T
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|||||||||
N-term | |||||||||
|
S
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A
|
T
|
P
|
N
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V
|
T
|
T
|
C
|
46 |
TM1 | |||||||||
P
|
M
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D
|
E
|
K
|
L
|
L
|
S
|
T
|
V
|
56 |
L
|
T
|
T
|
S
|
Y
|
S
|
V
|
I
|
F
|
I
|
66 |
V
|
G
|
L
|
V
|
G
|
N
|
I
|
I
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A
|
L
|
76 |
Y
|
V
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F
|
L
|
G
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I
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H
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R
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K
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R
|
86 |
TM2 | |||||||||
N
|
S
|
I
|
Q
|
I
|
Y
|
L
|
L
|
N
|
V
|
96 |
A
|
I
|
A
|
D
|
L
|
L
|
L
|
I
|
F
|
C
|
106 |
L
|
P
|
F
|
R
|
I
|
M
|
Y
|
H
|
I
|
N
|
116 |
ECL1 | TM3 | ||||||||
Q
|
N
|
K
|
W
|
T
|
L
|
G
|
V
|
I
|
L
|
126 |
C
|
K
|
V
|
V
|
G
|
T
|
L
|
F
|
Y
|
M
|
136 |
N
|
M
|
Y
|
I
|
S
|
I
|
I
|
L
|
L
|
G
|
146 |
F
|
I
|
S
|
L
|
D
|
R
|
Y
|
I
|
K
|
I
|
156 |
ICL2 | |||||||||
N
|
R
|
S
|
I
|
Q
|
Q
|
R
|
K
|
A
|
I
|
166 |
TM4 | |||||||||
T
|
T
|
K
|
Q
|
S
|
I
|
Y
|
V
|
C
|
C
|
176 |
I
|
V
|
W
|
M
|
L
|
A
|
L
|
G
|
G
|
F
|
186 |
L
|
T
|
M
|
I
|
I
|
L
|
T
|
L
|
K
|
K
|
196 |
ECL2 | |||||||||
G
|
G
|
H
|
N
|
S
|
T
|
M
|
C
|
F
|
H
|
206 |
TM5 | |||||||||
Y
|
R
|
D
|
K
|
H
|
N
|
A
|
K
|
G
|
E
|
216 |
A
|
I
|
F
|
N
|
F
|
I
|
L
|
V
|
V
|
M
|
226 |
F
|
W
|
L
|
I
|
F
|
L
|
L
|
I
|
I
|
L
|
236 |
S
|
Y
|
I
|
K
|
I
|
G
|
K
|
N
|
L
|
L
|
246 |
ICL3 | |||||||||
R
|
I
|
S
|
K
|
R
|
R
|
S
|
K
|
F
|
P
|
256 |
TM6 | |||||||||
N
|
S
|
G
|
K
|
Y
|
A
|
T
|
T
|
A
|
R
|
266 |
N
|
S
|
F
|
I
|
V
|
L
|
I
|
I
|
F
|
T
|
276 |
I
|
C
|
F
|
V
|
P
|
Y
|
H
|
A
|
F
|
R
|
286 |
ECL3 | |||||||||
F
|
I
|
Y
|
I
|
S
|
S
|
Q
|
L
|
N
|
V
|
296 |
TM7 | |||||||||
S
|
S
|
C
|
Y
|
W
|
K
|
E
|
I
|
V
|
H
|
306 |
K
|
T
|
N
|
E
|
I
|
M
|
L
|
V
|
L
|
S
|
316 |
S
|
F
|
N
|
S
|
C
|
L
|
D
|
P
|
V
|
M
|
326 |
H8 | |||||||||
Y
|
F
|
L
|
M
|
S
|
S
|
N
|
I
|
R
|
K
|
336 |
I
|
M
|
C
|
Q
|
L
|
L
|
F
|
R
|
R
|
F
|
346 |
C-term | |||||||||
Q
|
G
|
E
|
P
|
S
|
R
|
S
|
E
|
S
|
T
|
356 |
S
|
E
|
F
|
K
|
P
|
G
|
Y
|
S
|
L
|
H
|
366 |
|
|||||||
D
|
T
|
S
|
V
|
A
|
V
|
K
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
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DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|