gpr61_human
NAME
8TB7
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
gpr61_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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E
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S
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S
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P
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I
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P
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Q
|
S
|
S
|
10 |
G
|
N
|
S
|
S
|
T
|
L
|
G
|
R
|
V
|
P
|
20 |
Q
|
T
|
P
|
G
|
P
|
S
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T
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A
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S
|
G
|
30 |
TM1 | |||||||||
V
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P
|
E
|
V
|
G
|
L
|
R
|
D
|
V
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A
|
40 |
S
|
E
|
S
|
V
|
A
|
L
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F
|
F
|
M
|
L
|
50 |
L
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L
|
D
|
L
|
T
|
A
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V
|
A
|
G
|
N
|
60 |
A
|
A
|
V
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M
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A
|
V
|
I
|
A
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K
|
T
|
70 |
ICL1 | TM2 | ||||||||
P
|
A
|
L
|
R
|
K
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F
|
V
|
F
|
V
|
F
|
80 |
H
|
L
|
C
|
L
|
V
|
D
|
L
|
L
|
A
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A
|
90 |
L
|
T
|
L
|
M
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P
|
L
|
A
|
M
|
L
|
S
|
100 |
ECL1 | |||||||||
S
|
S
|
A
|
L
|
F
|
D
|
H
|
A
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L
|
F
|
110 |
TM3 | |||||||||
G
|
E
|
V
|
A
|
C
|
R
|
L
|
Y
|
L
|
F
|
120 |
L
|
S
|
V
|
C
|
F
|
V
|
S
|
L
|
A
|
I
|
130 |
L
|
S
|
V
|
S
|
A
|
I
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N
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V
|
E
|
R
|
140 |
ICL2 | |||||||||
Y
|
Y
|
Y
|
V
|
V
|
H
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P
|
M
|
R
|
Y
|
150 |
TM4 | |||||||||
E
|
V
|
R
|
M
|
T
|
L
|
G
|
L
|
V
|
A
|
160 |
S
|
V
|
L
|
V
|
G
|
V
|
W
|
V
|
K
|
A
|
170 |
L
|
A
|
M
|
A
|
S
|
V
|
P
|
V
|
L
|
G
|
180 |
ECL2 | |||||||||
R
|
V
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S
|
W
|
E
|
E
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G
|
A
|
P
|
S
|
190 |
V
|
P
|
P
|
G
|
C
|
S
|
L
|
Q
|
W
|
S
|
200 |
TM5 | |||||||||
H
|
S
|
A
|
Y
|
C
|
Q
|
L
|
F
|
V
|
V
|
210 |
V
|
F
|
A
|
V
|
L
|
Y
|
F
|
L
|
L
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P
|
220 |
L
|
L
|
L
|
I
|
L
|
V
|
V
|
Y
|
C
|
S
|
230 |
|
|
|
|
|
|
|
|||
M
|
F
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R
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V
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A
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R
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V
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A
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A
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M
|
240 |
|
|
|
|
|
|
|
|
|
|
ICL3 | |||||||||
Q
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H
|
G
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P
|
L
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P
|
T
|
W
|
M
|
E
|
250 |
|
|
|
|
|
|
|
|
|
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T
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P
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R
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Q
|
R
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S
|
E
|
S
|
L
|
S
|
260 |
|
|
|
|
|
|
|
|
|
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S
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R
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S
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T
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M
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V
|
T
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S
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S
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G
|
270 |
|
|
|
|
|
|
|
|
|
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TM6 | |||||||||
A
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P
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Q
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T
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T
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P
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H
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R
|
T
|
F
|
280 |
|
|
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|||||||
G
|
G
|
G
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K
|
A
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A
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V
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V
|
L
|
L
|
290 |
A
|
V
|
G
|
G
|
Q
|
F
|
L
|
L
|
C
|
W
|
300 |
L
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P
|
Y
|
F
|
S
|
F
|
H
|
L
|
Y
|
V
|
310 |
ECL3 | |||||||||
A
|
L
|
S
|
A
|
Q
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P
|
I
|
S
|
T
|
G
|
320 |
TM7 | |||||||||
Q
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V
|
E
|
S
|
V
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V
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T
|
W
|
I
|
G
|
330 |
Y
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F
|
C
|
F
|
T
|
S
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N
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P
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F
|
F
|
340 |
H8 | |||||||||
Y
|
G
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C
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L
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N
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R
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Q
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I
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R
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G
|
350 |
E
|
L
|
S
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K
|
Q
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F
|
V
|
C
|
F
|
F
|
360 |
C-term | |||||||||
K
|
P
|
A
|
P
|
E
|
E
|
E
|
L
|
R
|
L
|
370 |
P
|
S
|
R
|
E
|
G
|
S
|
I
|
E
|
E
|
N
|
380 |
F
|
L
|
Q
|
F
|
L
|
Q
|
G
|
T
|
G
|
C
|
390 |
P
|
S
|
E
|
S
|
W
|
V
|
S
|
R
|
P
|
L
|
400 |
P
|
S
|
P
|
K
|
Q
|
E
|
P
|
P
|
A
|
V
|
410 |
D
|
F
|
R
|
I
|
P
|
G
|
Q
|
I
|
A
|
E
|
420 |
E
|
T
|
S
|
E
|
F
|
L
|
E
|
Q
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Q
|
L
|
430 |
T
|
S
|
D
|
I
|
I
|
M
|
S
|
D
|
S
|
Y
|
440 |
L
|
R
|
P
|
A
|
A
|
S
|
P
|
R
|
L
|
E
|
450 |
S
|
|
|||||||||
N-term | |||||||||
|
E
|
S
|
S
|
P
|
I
|
P
|
Q
|
S
|
S
|
10 |
G
|
N
|
S
|
S
|
T
|
L
|
G
|
R
|
V
|
P
|
20 |
Q
|
T
|
P
|
G
|
P
|
S
|
T
|
A
|
S
|
G
|
30 |
TM1 | |||||||||
V
|
P
|
E
|
V
|
G
|
L
|
R
|
D
|
V
|
A
|
40 |
S
|
E
|
S
|
V
|
A
|
L
|
F
|
F
|
M
|
L
|
50 |
L
|
L
|
D
|
L
|
T
|
A
|
V
|
A
|
G
|
N
|
60 |
A
|
A
|
V
|
M
|
A
|
V
|
I
|
A
|
K
|
T
|
70 |
ICL1 | TM2 | ||||||||
P
|
A
|
L
|
R
|
K
|
F
|
V
|
F
|
V
|
F
|
80 |
H
|
L
|
C
|
L
|
V
|
D
|
L
|
L
|
A
|
A
|
90 |
L
|
T
|
L
|
M
|
P
|
L
|
A
|
M
|
L
|
S
|
100 |
ECL1 | |||||||||
S
|
S
|
A
|
L
|
F
|
D
|
H
|
A
|
L
|
F
|
110 |
TM3 | |||||||||
G
|
E
|
V
|
A
|
C
|
R
|
L
|
Y
|
L
|
F
|
120 |
L
|
S
|
V
|
C
|
F
|
V
|
S
|
L
|
A
|
I
|
130 |
L
|
S
|
V
|
S
|
A
|
I
|
N
|
V
|
E
|
R
|
140 |
ICL2 | |||||||||
Y
|
Y
|
Y
|
V
|
V
|
H
|
P
|
M
|
R
|
Y
|
150 |
TM4 | |||||||||
E
|
V
|
R
|
M
|
T
|
L
|
G
|
L
|
V
|
A
|
160 |
S
|
V
|
L
|
V
|
G
|
V
|
W
|
V
|
K
|
A
|
170 |
L
|
A
|
M
|
A
|
S
|
V
|
P
|
V
|
L
|
G
|
180 |
ECL2 | |||||||||
R
|
V
|
S
|
W
|
E
|
E
|
G
|
A
|
P
|
S
|
190 |
V
|
P
|
P
|
G
|
C
|
S
|
L
|
Q
|
W
|
S
|
200 |
TM5 | |||||||||
H
|
S
|
A
|
Y
|
C
|
Q
|
L
|
F
|
V
|
V
|
210 |
V
|
F
|
A
|
V
|
L
|
Y
|
F
|
L
|
L
|
P
|
220 |
L
|
L
|
L
|
I
|
L
|
V
|
V
|
Y
|
C
|
S
|
230 |
|
|||||||||
TM6 | |||||||||
M
|
F
|
R
|
|
K
|
A
|
A
|
V
|
V
|
L
|
289 |
L
|
A
|
V
|
G
|
G
|
Q
|
F
|
L
|
L
|
C
|
299 |
W
|
L
|
P
|
Y
|
F
|
S
|
F
|
H
|
L
|
Y
|
309 |
ECL3 | |||||||||
V
|
A
|
L
|
S
|
A
|
Q
|
P
|
I
|
S
|
T
|
319 |
TM7 | |||||||||
G
|
Q
|
V
|
E
|
S
|
V
|
V
|
T
|
W
|
I
|
329 |
G
|
Y
|
F
|
C
|
F
|
T
|
S
|
N
|
P
|
F
|
339 |
H8 | |||||||||
F
|
Y
|
G
|
C
|
L
|
N
|
R
|
Q
|
I
|
R
|
349 |
G
|
E
|
L
|
S
|
K
|
Q
|
F
|
V
|
C
|
F
|
359 |
C-term | |||||||||
F
|
K
|
P
|
A
|
P
|
E
|
E
|
E
|
L
|
R
|
369 |
L
|
P
|
S
|
R
|
E
|
G
|
S
|
I
|
E
|
E
|
379 |
N
|
F
|
L
|
Q
|
F
|
L
|
Q
|
G
|
T
|
G
|
389 |
C
|
P
|
S
|
E
|
S
|
W
|
V
|
S
|
R
|
P
|
399 |
L
|
P
|
S
|
P
|
K
|
Q
|
E
|
P
|
P
|
A
|
409 |
V
|
D
|
F
|
R
|
I
|
P
|
G
|
Q
|
I
|
A
|
419 |
E
|
E
|
T
|
S
|
E
|
F
|
L
|
E
|
Q
|
Q
|
429 |
L
|
T
|
S
|
D
|
I
|
I
|
M
|
S
|
D
|
S
|
439 |
Y
|
L
|
R
|
P
|
A
|
A
|
S
|
P
|
R
|
L
|
449 |
E
|
S
|
451 |
SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
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DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|