casr_human
NAME
8WPG
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
casr_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
N-term | |||||||||
M
|
A
|
F
|
Y
|
S
|
C
|
C
|
W
|
V
|
L
|
10 |
|
|
|
|
|
|
|
|
|
|
L
|
A
|
L
|
T
|
W
|
H
|
T
|
S
|
A
|
Y
|
20 |
G
|
P
|
D
|
Q
|
R
|
A
|
Q
|
K
|
K
|
G
|
30 |
D
|
I
|
I
|
L
|
G
|
G
|
L
|
F
|
P
|
I
|
40 |
H
|
F
|
G
|
V
|
A
|
A
|
K
|
D
|
Q
|
D
|
50 |
L
|
K
|
S
|
R
|
P
|
E
|
S
|
V
|
E
|
C
|
60 |
I
|
R
|
Y
|
N
|
F
|
R
|
G
|
F
|
R
|
W
|
70 |
L
|
Q
|
A
|
M
|
I
|
F
|
A
|
I
|
E
|
E
|
80 |
I
|
N
|
S
|
S
|
P
|
A
|
L
|
L
|
P
|
N
|
90 |
L
|
T
|
L
|
G
|
Y
|
R
|
I
|
F
|
D
|
T
|
100 |
C
|
N
|
T
|
V
|
S
|
K
|
A
|
L
|
E
|
A
|
110 |
T
|
L
|
S
|
F
|
V
|
A
|
Q
|
N
|
K
|
I
|
120 |
D
|
S
|
L
|
N
|
L
|
D
|
E
|
F
|
C
|
N
|
130 |
C
|
S
|
E
|
H
|
I
|
P
|
S
|
T
|
I
|
A
|
140 |
V
|
V
|
G
|
A
|
T
|
G
|
S
|
G
|
V
|
S
|
150 |
T
|
A
|
V
|
A
|
N
|
L
|
L
|
G
|
L
|
F
|
160 |
Y
|
I
|
P
|
Q
|
V
|
S
|
Y
|
A
|
S
|
S
|
170 |
S
|
R
|
L
|
L
|
S
|
N
|
K
|
N
|
Q
|
F
|
180 |
K
|
S
|
F
|
L
|
R
|
T
|
I
|
P
|
N
|
D
|
190 |
E
|
H
|
Q
|
A
|
T
|
A
|
M
|
A
|
D
|
I
|
200 |
I
|
E
|
Y
|
F
|
R
|
W
|
N
|
W
|
V
|
G
|
210 |
T
|
I
|
A
|
A
|
D
|
D
|
D
|
Y
|
G
|
R
|
220 |
P
|
G
|
I
|
E
|
K
|
F
|
R
|
E
|
E
|
A
|
230 |
E
|
E
|
R
|
D
|
I
|
C
|
I
|
D
|
F
|
S
|
240 |
E
|
L
|
I
|
S
|
Q
|
Y
|
S
|
D
|
E
|
E
|
250 |
E
|
I
|
Q
|
H
|
V
|
V
|
E
|
V
|
I
|
Q
|
260 |
N
|
S
|
T
|
A
|
K
|
V
|
I
|
V
|
V
|
F
|
270 |
S
|
S
|
G
|
P
|
D
|
L
|
E
|
P
|
L
|
I
|
280 |
K
|
E
|
I
|
V
|
R
|
R
|
N
|
I
|
T
|
G
|
290 |
K
|
I
|
W
|
L
|
A
|
S
|
E
|
A
|
W
|
A
|
300 |
S
|
S
|
S
|
L
|
I
|
A
|
M
|
P
|
Q
|
Y
|
310 |
F
|
H
|
V
|
V
|
G
|
G
|
T
|
I
|
G
|
F
|
320 |
A
|
L
|
K
|
A
|
G
|
Q
|
I
|
P
|
G
|
F
|
330 |
R
|
E
|
F
|
L
|
K
|
K
|
V
|
H
|
P
|
R
|
340 |
K
|
S
|
V
|
H
|
N
|
G
|
F
|
A
|
K
|
E
|
350 |
F
|
W
|
E
|
E
|
T
|
F
|
N
|
C
|
H
|
L
|
360 |
Q
|
E
|
G
|
A
|
K
|
G
|
P
|
L
|
P
|
V
|
370 |
D
|
T
|
F
|
L
|
R
|
G
|
H
|
E
|
E
|
S
|
380 |
G
|
D
|
R
|
F
|
S
|
N
|
S
|
S
|
T
|
A
|
390 |
F
|
R
|
P
|
L
|
C
|
T
|
G
|
D
|
E
|
N
|
400 |
I
|
S
|
S
|
V
|
E
|
T
|
P
|
Y
|
I
|
D
|
410 |
Y
|
T
|
H
|
L
|
R
|
I
|
S
|
Y
|
N
|
V
|
420 |
Y
|
L
|
A
|
V
|
Y
|
S
|
I
|
A
|
H
|
A
|
430 |
L
|
Q
|
D
|
I
|
Y
|
T
|
C
|
L
|
P
|
G
|
440 |
R
|
G
|
L
|
F
|
T
|
N
|
G
|
S
|
C
|
A
|
450 |
D
|
I
|
K
|
K
|
V
|
E
|
A
|
W
|
Q
|
V
|
460 |
L
|
K
|
H
|
L
|
R
|
H
|
L
|
N
|
F
|
T
|
470 |
N
|
N
|
M
|
G
|
E
|
Q
|
V
|
T
|
F
|
D
|
480 |
E
|
C
|
G
|
D
|
L
|
V
|
G
|
N
|
Y
|
S
|
490 |
I
|
I
|
N
|
W
|
H
|
L
|
S
|
P
|
E
|
D
|
500 |
G
|
S
|
I
|
V
|
F
|
K
|
E
|
V
|
G
|
Y
|
510 |
Y
|
N
|
V
|
Y
|
A
|
K
|
K
|
G
|
E
|
R
|
520 |
L
|
F
|
I
|
N
|
E
|
E
|
K
|
I
|
L
|
W
|
530 |
S
|
G
|
F
|
S
|
R
|
E
|
V
|
P
|
F
|
S
|
540 |
N
|
C
|
S
|
R
|
D
|
C
|
L
|
A
|
G
|
T
|
550 |
R
|
K
|
G
|
I
|
I
|
E
|
G
|
E
|
P
|
T
|
560 |
C
|
C
|
F
|
E
|
C
|
V
|
E
|
C
|
P
|
D
|
570 |
G
|
E
|
Y
|
S
|
D
|
E
|
T
|
D
|
A
|
S
|
580 |
A
|
C
|
N
|
K
|
C
|
P
|
D
|
D
|
F
|
W
|
590 |
S
|
N
|
E
|
N
|
H
|
T
|
S
|
C
|
I
|
A
|
600 |
K
|
E
|
I
|
E
|
F
|
L
|
S
|
W
|
T
|
E
|
610 |
TM1 | |||||||||
P
|
F
|
G
|
I
|
A
|
L
|
T
|
L
|
F
|
A
|
620 |
V
|
L
|
G
|
I
|
F
|
L
|
T
|
A
|
F
|
V
|
630 |
L
|
G
|
V
|
F
|
I
|
K
|
F
|
R
|
N
|
T
|
640 |
ICL1 | TM2 | ||||||||
P
|
I
|
V
|
K
|
A
|
T
|
N
|
R
|
E
|
L
|
650 |
S
|
Y
|
L
|
L
|
L
|
F
|
S
|
L
|
L
|
C
|
660 |
C
|
F
|
S
|
S
|
S
|
L
|
F
|
F
|
I
|
G
|
670 |
ECL1 | TM3 | ||||||||
E
|
P
|
Q
|
D
|
W
|
T
|
C
|
R
|
L
|
R
|
680 |
Q
|
P
|
A
|
F
|
G
|
I
|
S
|
F
|
V
|
L
|
690 |
C
|
I
|
S
|
C
|
I
|
L
|
V
|
K
|
T
|
N
|
700 |
ICL2 | |||||||||
R
|
V
|
L
|
L
|
V
|
F
|
E
|
A
|
K
|
I
|
710 |
P
|
T
|
S
|
F
|
H
|
R
|
K
|
W
|
W
|
G
|
720 |
TM4 | |||||||||
L
|
N
|
L
|
Q
|
F
|
L
|
L
|
V
|
F
|
L
|
730 |
C
|
T
|
F
|
M
|
Q
|
I
|
V
|
I
|
C
|
V
|
740 |
ECL2 | |||||||||
I
|
W
|
L
|
Y
|
T
|
A
|
P
|
P
|
S
|
S
|
750 |
Y
|
R
|
N
|
Q
|
E
|
L
|
E
|
D
|
E
|
I
|
760 |
I
|
F
|
I
|
T
|
C
|
H
|
E
|
G
|
S
|
L
|
770 |
TM5 | |||||||||
M
|
A
|
L
|
G
|
F
|
L
|
I
|
G
|
Y
|
T
|
780 |
C
|
L
|
L
|
A
|
A
|
I
|
C
|
F
|
F
|
F
|
790 |
A
|
F
|
K
|
S
|
R
|
K
|
L
|
P
|
E
|
N
|
800 |
ICL3 | TM6 | ||||||||
F
|
N
|
E
|
A
|
K
|
F
|
I
|
T
|
F
|
S
|
810 |
M
|
L
|
I
|
F
|
F
|
I
|
V
|
W
|
I
|
S
|
820 |
F
|
I
|
P
|
A
|
Y
|
A
|
S
|
T
|
Y
|
G
|
830 |
TM7 | |||||||||
K
|
F
|
V
|
S
|
A
|
V
|
E
|
V
|
I
|
A
|
840 |
I
|
L
|
A
|
A
|
S
|
F
|
G
|
L
|
L
|
A
|
850 |
C
|
I
|
F
|
F
|
N
|
K
|
I
|
Y
|
I
|
I
|
860 |
H8 | |||||||||
L
|
F
|
K
|
P
|
S
|
R
|
N
|
T
|
I
|
E
|
870 |
E
|
V
|
R
|
C
|
S
|
T
|
A
|
A
|
H
|
A
|
880 |
C-term | |||||||||
F
|
K
|
V
|
A
|
A
|
R
|
A
|
T
|
L
|
R
|
890 |
|
|
|
|
|
|
|
|
||
R
|
S
|
N
|
V
|
S
|
R
|
K
|
R
|
S
|
S
|
900 |
|
|
|
|
|
|
|
|
|
|
S
|
L
|
G
|
G
|
S
|
T
|
G
|
S
|
T
|
P
|
910 |
|
|
|
|
|
|
|
|
|
|
S
|
S
|
S
|
I
|
S
|
S
|
K
|
S
|
N
|
S
|
920 |
|
|
|
|
|
|
|
|
|
|
E
|
D
|
P
|
F
|
P
|
Q
|
P
|
E
|
R
|
Q
|
930 |
|
|
|
|
|
|
|
|
|
|
K
|
Q
|
Q
|
Q
|
P
|
L
|
A
|
L
|
T
|
Q
|
940 |
|
|
|
|
|
|
|
|
|
|
Q
|
E
|
Q
|
Q
|
Q
|
Q
|
P
|
L
|
T
|
L
|
950 |
|
|
|
|
|
|
|
|
|
|
P
|
Q
|
Q
|
Q
|
R
|
S
|
Q
|
Q
|
Q
|
P
|
960 |
|
|
|
|
|
|
|
|
|
|
R
|
C
|
K
|
Q
|
K
|
V
|
I
|
F
|
G
|
S
|
970 |
|
|
|
|
|
|
|
|
|
|
G
|
T
|
V
|
T
|
F
|
S
|
L
|
S
|
F
|
D
|
980 |
|
|
|
|
|
|
|
|
|
|
E
|
P
|
Q
|
K
|
N
|
A
|
M
|
A
|
H
|
R
|
990 |
|
|
|
|
|
|
|
|
|
|
N
|
S
|
T
|
H
|
Q
|
N
|
S
|
L
|
E
|
A
|
1000 |
|
|
|
|
|
|
|
|
|
|
Q
|
K
|
S
|
S
|
D
|
T
|
L
|
T
|
R
|
H
|
1010 |
|
|
|
|
|
|
|
|
|
|
E
|
P
|
L
|
L
|
P
|
L
|
Q
|
C
|
G
|
E
|
1020 |
|
|
|
|
|
|
|
|
|
|
T
|
D
|
L
|
D
|
L
|
T
|
V
|
Q
|
E
|
T
|
1030 |
|
|
|
|
|
|
|
|
|
|
G
|
L
|
Q
|
G
|
P
|
V
|
G
|
G
|
D
|
Q
|
1040 |
|
|
|
|
|
|
|
|
|
|
R
|
P
|
E
|
V
|
E
|
D
|
P
|
E
|
E
|
L
|
1050 |
|
|
|
|
|
|
|
|
|
|
S
|
P
|
A
|
L
|
V
|
V
|
S
|
S
|
S
|
Q
|
1060 |
|
|
|
|
|
|
|
|
|
|
S
|
F
|
V
|
I
|
S
|
G
|
G
|
G
|
S
|
T
|
1070 |
|
|
|
|
|
|
|
|
V
|
T
|
E
|
N
|
V
|
V
|
N
|
S
|
|
|||||||||
N-term | |||||||||
|
Y
|
G
|
P
|
D
|
Q
|
R
|
A
|
Q
|
K
|
28 |
K
|
G
|
D
|
I
|
I
|
L
|
G
|
G
|
L
|
F
|
38 |
P
|
I
|
H
|
F
|
G
|
V
|
A
|
A
|
K
|
D
|
48 |
Q
|
D
|
L
|
K
|
S
|
R
|
P
|
E
|
S
|
V
|
58 |
E
|
C
|
I
|
R
|
Y
|
N
|
F
|
R
|
G
|
F
|
68 |
R
|
W
|
L
|
Q
|
A
|
M
|
I
|
F
|
A
|
I
|
78 |
E
|
E
|
I
|
N
|
S
|
S
|
P
|
A
|
L
|
L
|
88 |
P
|
N
|
L
|
T
|
L
|
G
|
Y
|
R
|
I
|
F
|
98 |
D
|
T
|
C
|
N
|
T
|
V
|
S
|
K
|
A
|
L
|
108 |
E
|
A
|
T
|
L
|
S
|
F
|
V
|
A
|
Q
|
N
|
118 |
K
|
I
|
D
|
S
|
L
|
N
|
L
|
D
|
E
|
F
|
128 |
C
|
N
|
C
|
S
|
E
|
H
|
I
|
P
|
S
|
T
|
138 |
I
|
A
|
V
|
V
|
G
|
A
|
T
|
G
|
S
|
G
|
148 |
V
|
S
|
T
|
A
|
V
|
A
|
N
|
L
|
L
|
G
|
158 |
L
|
F
|
Y
|
I
|
P
|
Q
|
V
|
S
|
Y
|
A
|
168 |
S
|
S
|
S
|
R
|
L
|
L
|
S
|
N
|
K
|
N
|
178 |
Q
|
F
|
K
|
S
|
F
|
L
|
R
|
T
|
I
|
P
|
188 |
N
|
D
|
E
|
H
|
Q
|
A
|
T
|
A
|
M
|
A
|
198 |
D
|
I
|
I
|
E
|
Y
|
F
|
R
|
W
|
N
|
W
|
208 |
V
|
G
|
T
|
I
|
A
|
A
|
D
|
D
|
D
|
Y
|
218 |
G
|
R
|
P
|
G
|
I
|
E
|
K
|
F
|
R
|
E
|
228 |
E
|
A
|
E
|
E
|
R
|
D
|
I
|
C
|
I
|
D
|
238 |
F
|
S
|
E
|
L
|
I
|
S
|
Q
|
Y
|
S
|
D
|
248 |
E
|
E
|
E
|
I
|
Q
|
H
|
V
|
V
|
E
|
V
|
258 |
I
|
Q
|
N
|
S
|
T
|
A
|
K
|
V
|
I
|
V
|
268 |
V
|
F
|
S
|
S
|
G
|
P
|
D
|
L
|
E
|
P
|
278 |
L
|
I
|
K
|
E
|
I
|
V
|
R
|
R
|
N
|
I
|
288 |
T
|
G
|
K
|
I
|
W
|
L
|
A
|
S
|
E
|
A
|
298 |
W
|
A
|
S
|
S
|
S
|
L
|
I
|
A
|
M
|
P
|
308 |
Q
|
Y
|
F
|
H
|
V
|
V
|
G
|
G
|
T
|
I
|
318 |
G
|
F
|
A
|
L
|
K
|
A
|
G
|
Q
|
I
|
P
|
328 |
G
|
F
|
R
|
E
|
F
|
L
|
K
|
K
|
V
|
H
|
338 |
P
|
R
|
K
|
S
|
V
|
H
|
N
|
G
|
F
|
A
|
348 |
K
|
E
|
F
|
W
|
E
|
E
|
T
|
F
|
N
|
C
|
358 |
H
|
L
|
Q
|
E
|
G
|
A
|
K
|
G
|
P
|
L
|
368 |
P
|
V
|
D
|
T
|
F
|
L
|
R
|
G
|
H
|
E
|
378 |
E
|
S
|
G
|
D
|
R
|
F
|
S
|
N
|
S
|
S
|
388 |
T
|
A
|
F
|
R
|
P
|
L
|
C
|
T
|
G
|
D
|
398 |
E
|
N
|
I
|
S
|
S
|
V
|
E
|
T
|
P
|
Y
|
408 |
I
|
D
|
Y
|
T
|
H
|
L
|
R
|
I
|
S
|
Y
|
418 |
N
|
V
|
Y
|
L
|
A
|
V
|
Y
|
S
|
I
|
A
|
428 |
H
|
A
|
L
|
Q
|
D
|
I
|
Y
|
T
|
C
|
L
|
438 |
P
|
G
|
R
|
G
|
L
|
F
|
T
|
N
|
G
|
S
|
448 |
C
|
A
|
D
|
I
|
K
|
K
|
V
|
E
|
A
|
W
|
458 |
Q
|
V
|
L
|
K
|
H
|
L
|
R
|
H
|
L
|
N
|
468 |
F
|
T
|
N
|
N
|
M
|
G
|
E
|
Q
|
V
|
T
|
478 |
F
|
D
|
E
|
C
|
G
|
D
|
L
|
V
|
G
|
N
|
488 |
Y
|
S
|
I
|
I
|
N
|
W
|
H
|
L
|
S
|
P
|
498 |
E
|
D
|
G
|
S
|
I
|
V
|
F
|
K
|
E
|
V
|
508 |
G
|
Y
|
Y
|
N
|
V
|
Y
|
A
|
K
|
K
|
G
|
518 |
E
|
R
|
L
|
F
|
I
|
N
|
E
|
E
|
K
|
I
|
528 |
L
|
W
|
S
|
G
|
F
|
S
|
R
|
E
|
V
|
P
|
538 |
F
|
S
|
N
|
C
|
S
|
R
|
D
|
C
|
L
|
A
|
548 |
G
|
T
|
R
|
K
|
G
|
I
|
I
|
E
|
G
|
E
|
558 |
P
|
T
|
C
|
C
|
F
|
E
|
C
|
V
|
E
|
C
|
568 |
P
|
D
|
G
|
E
|
Y
|
S
|
D
|
E
|
T
|
D
|
578 |
A
|
S
|
A
|
C
|
N
|
K
|
C
|
P
|
D
|
D
|
588 |
F
|
W
|
S
|
N
|
E
|
N
|
H
|
T
|
S
|
C
|
598 |
I
|
A
|
K
|
E
|
I
|
E
|
F
|
L
|
S
|
W
|
608 |
TM1 | |||||||||
T
|
E
|
P
|
F
|
G
|
I
|
A
|
L
|
T
|
L
|
618 |
F
|
A
|
V
|
L
|
G
|
I
|
F
|
L
|
T
|
A
|
628 |
F
|
V
|
L
|
G
|
V
|
F
|
I
|
K
|
F
|
R
|
638 |
ICL1 | |||||||||
N
|
T
|
P
|
I
|
V
|
K
|
A
|
T
|
N
|
R
|
648 |
TM2 | |||||||||
E
|
L
|
S
|
Y
|
L
|
L
|
L
|
F
|
S
|
L
|
658 |
L
|
C
|
C
|
F
|
S
|
S
|
S
|
L
|
F
|
F
|
668 |
ECL1 | TM3 | ||||||||
I
|
G
|
E
|
P
|
Q
|
D
|
W
|
T
|
C
|
R
|
678 |
L
|
R
|
Q
|
P
|
A
|
F
|
G
|
I
|
S
|
F
|
688 |
V
|
L
|
C
|
I
|
S
|
C
|
I
|
L
|
V
|
K
|
698 |
T
|
N
|
R
|
V
|
L
|
L
|
V
|
F
|
E
|
A
|
708 |
ICL2 | |||||||||
K
|
I
|
P
|
T
|
S
|
F
|
H
|
R
|
K
|
W
|
718 |
TM4 | |||||||||
W
|
G
|
L
|
N
|
L
|
Q
|
F
|
L
|
L
|
V
|
728 |
F
|
L
|
C
|
T
|
F
|
M
|
Q
|
I
|
V
|
I
|
738 |
C
|
V
|
I
|
W
|
L
|
Y
|
T
|
A
|
P
|
P
|
748 |
ECL2 | |||||||||
S
|
S
|
Y
|
R
|
N
|
Q
|
E
|
L
|
E
|
D
|
758 |
E
|
I
|
I
|
F
|
I
|
T
|
C
|
H
|
E
|
G
|
768 |
TM5 | |||||||||
S
|
L
|
M
|
A
|
L
|
G
|
F
|
L
|
I
|
G
|
778 |
Y
|
T
|
C
|
L
|
L
|
A
|
A
|
I
|
C
|
F
|
788 |
F
|
F
|
A
|
F
|
K
|
S
|
R
|
K
|
L
|
P
|
798 |
ICL3 | TM6 | ||||||||
E
|
N
|
F
|
N
|
E
|
A
|
K
|
F
|
I
|
T
|
808 |
F
|
S
|
M
|
L
|
I
|
F
|
F
|
I
|
V
|
W
|
818 |
I
|
S
|
F
|
I
|
P
|
A
|
Y
|
A
|
S
|
T
|
828 |
ECL3 | TM7 | ||||||||
Y
|
G
|
K
|
F
|
V
|
S
|
A
|
V
|
E
|
V
|
838 |
I
|
A
|
I
|
L
|
A
|
A
|
S
|
F
|
G
|
L
|
848 |
L
|
A
|
C
|
I
|
F
|
F
|
N
|
K
|
I
|
Y
|
858 |
H8 | |||||||||
I
|
I
|
L
|
F
|
K
|
P
|
S
|
R
|
N
|
T
|
868 |
I
|
E
|
E
|
V
|
R
|
C
|
S
|
T
|
A
|
A
|
878 |
C-term | |||||||||
H
|
A
|
F
|
K
|
V
|
A
|
A
|
R
|
A
|
T
|
888 |
|
||||
L
|
R
|
R
|
S
|
|
SCHEMATIC
Perspective:
Wild-type
Construct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
SOLUBILIZATION
Solvent type | Item | Concentration |
---|
Remarks:
PURIFICATION
Purification type | Description |
---|
Remarks:
CRYSTALLIZATION
METHOD:
TYPE: ()
PROTEIN CONCENTRATION:
Temperature:
ph : -
Remarks:
Chemical lists
Type | Item | Concentration |
---|