GPCRdb

Ligand source activities (1 row/activity)

Potency
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Ligands					Receptor			Assay information							Chemical information
Sel. page	Common name	GPCRdb ID	#Vendors	Reference ligand	Fold selectivity (Potency)	# tested GPCRs (Potency)	Species	p-value (-log)	Type	Activity Relation	Activity Value	Assay Type	Assay Description	Source	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles	DOI
	155524115	175973	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175973	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175973	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	155524115	175973	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175973	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175973	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	155524115	175973	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175973	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175973	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	46235428	6526	0	None	263	3	Human	9.3	pEC50	=	9.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6526	0	None	263	3	Human	9.3	pEC50	=	9.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	58227401	175877	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175877	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175877	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	58227401	175877	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175877	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175877	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	51354300	58581	0	None	-	1	Human	9.0	pEC50	=	9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688946	58581	0	None	-	1	Human	9.0	pEC50	=	9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	155491015	175921	3	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	175921	3	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	175921	3	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	155513612	175746	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	58227401	175877	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175877	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175877	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	45257132	3626	3	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3626	3	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3626	3	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	155552061	175904	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175904	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175904	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	155517880	176093	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176093	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176093	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	45257132	3626	3	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3626	3	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3626	3	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	58227373	175934	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175934	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175934	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	136030655	56188	0	None	1	3	Mouse	8.0	pEC50	=	8	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56188	0	None	1	3	Mouse	8.0	pEC50	=	8	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136030655	56188	0	None	-1	3	Rat	8.0	pEC50	=	8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56188	0	None	-1	3	Rat	8.0	pEC50	=	8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25068753	187854	15	None	-1	2	Rat	8.0	pEC50	=	8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187854	15	None	-1	2	Rat	8.0	pEC50	=	8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	10105117	103718	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL309819	103718	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	127051301	139670	0	None	4	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139670	0	None	4	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	136141537	56187	1	None	1	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56187	1	None	1	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	4024	1984	14	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	9989505	1984	14	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL82298	1984	14	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	58227259	116640	0	None	-	1	Human	6.0	pEC50	=	6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	116640	0	None	-	1	Human	6.0	pEC50	=	6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1194	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	23786499	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	CHEMBL26116	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	24797598	61233	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771001	61233	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	11846299	138690	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	CHEMBL378788	138690	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	46228114	198492	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596551	198492	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25130576	187043	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187043	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130232	186980	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cn(C)cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494576	186980	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cn(C)cn3)nc(N)n2)CC1	10.1021/jm8005959
	25131229	187012	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	CHEMBL494724	187012	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	25130576	187043	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187043	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	11846635	78270	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	CHEMBL211242	78270	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	46228132	200459	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL609161	200459	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	25129178	187046	1	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL494910	187046	1	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	1267	3744	43	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	3035905	3744	43	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	CHEMBL260374	3744	43	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	44480708	5698	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(/NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1021/jm900526h
	CHEMBL1078632	5698	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(/NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1021/jm900526h
	53233804	61197	0	None	-	1	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770966	61197	0	None	-	1	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	1204	1901	114	None	-1	10	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1901	114	None	-1	10	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1901	114	None	-1	10	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1901	114	None	-1	10	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1901	114	None	-1	10	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1901	114	None	-1	10	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	127052293	139708	1	None	10	2	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139708	1	None	10	2	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1247	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1375	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	774	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	CHEMBL90	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	DB05381	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	45257131	198226	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	198226	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	56593499	139712	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	6	3	4	3.2	N#C/N=C(/NCCSc1ccccc1)N[C@H]1CC[C@@H](c2c[nH]cn2)CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805392	139712	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	6	3	4	3.2	N#C/N=C(/NCCSc1ccccc1)N[C@H]1CC[C@@H](c2c[nH]cn2)CC1	10.1021/acs.jmedchem.6b00120
	127050776	139717	0	None	3	3	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	139717	0	None	3	3	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	127050431	139785	0	None	3	2	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	139785	0	None	3	2	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227165	116620	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393536	116620	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	25130578	186750	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	251	4	1	5	1.4	CN(C)CCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL493099	186750	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	251	4	1	5	1.4	CN(C)CCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	25129525	187000	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494648	187000	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	25128833	187818	30	None	-2	2	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	CHEMBL501882	187818	30	None	-2	2	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	25128831	172044	0	None	1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL449157	172044	0	None	1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	11043445	56185	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL1632410	56185	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	16061839	187834	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	284	3	2	6	1.6	CN1CCN(c2cc(Nc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL502104	187834	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	284	3	2	6	1.6	CN1CCN(c2cc(Nc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	127050431	139785	0	None	3	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	139785	0	None	3	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	54585780	61229	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770998	61229	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	1204	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	1247	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	1375	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	774	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	CHEMBL90	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	DB05381	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	25130914	186671	0	None	15	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL492677	186671	0	None	15	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	11957573	1979	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	1250	1979	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	3692	1979	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	CHEMBL19439	1979	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	11957573	1979	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1250	1979	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3692	1979	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19439	1979	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1253	2027	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	132937	2027	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19010	2027	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10176414	4000	13	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1246	4000	13	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL281576	4000	13	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1204	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1267	3744	43	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	44482032	5655	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	262	7	3	3	1.4	CC(C)CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1078346	5655	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	262	7	3	3	1.4	CC(C)CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	25130581	186642	0	None	3	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL492489	186642	0	None	3	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	44482029	5560	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1cccnc1	10.1021/jm900526h
	CHEMBL1077737	5560	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1cccnc1	10.1021/jm900526h
	25131234	187079	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccccc3C#N)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495083	187079	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccccc3C#N)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187004	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187004	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187004	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187004	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	25130234	186820	0	None	-	1	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	2	6	-0.7	CN1CCN(c2cc(N3CCNC3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493663	186820	0	None	-	1	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	2	6	-0.7	CN1CCN(c2cc(N3CCNC3=O)nc(N)n2)CC1	10.1021/jm8005959
	127051986	139774	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806098	139774	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	51354301	58584	0	None	-1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	CHEMBL1688969	58584	0	None	-1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	10934158	108090	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL320331	108090	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	10017821	103310	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL308913	103310	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1238	2051	11	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	6603865	2051	11	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL11919	2051	11	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	11043445	56185	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632410	56185	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	10945113	109141	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL322256	109141	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	44480707	5686	0	None	5	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	328	8	3	4	2.2	N#C/N=C(/NCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078529	5686	0	None	5	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	328	8	3	4	2.2	N#C/N=C(/NCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	10945113	109141	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL322256	109141	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	127052294	139772	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	139772	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1247	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1375	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	774	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	CHEMBL90	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	DB05381	1901	114	None	-1	10	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	10934158	108090	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL320331	108090	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	25129182	186700	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	291	2	1	7	-0.4	CN1CCN(c2cc(N3CCN(C)CC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492869	186700	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	291	2	1	7	-0.4	CN1CCN(c2cc(N3CCN(C)CC3)nc(N)n2)CC1	10.1021/jm8005959
	127052293	139708	1	None	10	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139708	1	None	10	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11846634	77655	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209758	77655	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	11846464	78051	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	CHEMBL211170	78051	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	25071319	188474	8	None	1	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188474	8	None	1	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	44482030	5599	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	338	9	3	3	2.9	CC(CCN/C(=N\C#N)NCCCCc1c[nH]cn1)c1ccccc1	10.1021/jm900526h
	CHEMBL1077860	5599	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	338	9	3	3	2.9	CC(CCN/C(=N\C#N)NCCCCc1c[nH]cn1)c1ccccc1	10.1021/jm900526h
	25131561	186821	0	None	-3	2	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493664	186821	0	None	-3	2	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	135897837	187045	12	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	209	1	2	6	-0.5	CN1CCN(c2cc(O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494909	187045	12	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	209	1	2	6	-0.5	CN1CCN(c2cc(O)nc(N)n2)CC1	10.1021/jm8005959
	127048828	139790	0	None	38	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806255	139790	0	None	38	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227255	116623	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393539	116623	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	44568832	186600	0	None	-	1	Rat	7.8	pEC50	=	7.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	2	1	6	1.2	CN1CCN(c2cc(-c3ccc(F)nc3F)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492242	186600	0	None	-	1	Rat	7.8	pEC50	=	7.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	2	1	6	1.2	CN1CCN(c2cc(-c3ccc(F)nc3F)nc(N)n2)CC1	10.1021/jm8005959
	25128822	186898	0	None	19	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	186898	0	None	19	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	1251	1983	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	3035842	1983	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	CHEMBL18661	1983	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	1251	1983	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	3035842	1983	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL18661	1983	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	25149712	178408	0	None	-6	3	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	299	7	4	3	1.6	N=C(NCCCc1c[nH]cn1)NC(=O)CCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL470790	178408	0	None	-6	3	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	299	7	4	3	1.6	N=C(NCCCc1c[nH]cn1)NC(=O)CCc1ccccc1	10.1016/j.bmcl.2010.10.041
	25149335	183643	0	None	-6	3	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL483406	183643	0	None	-6	3	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	1278	2119	79	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2119	79	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2119	79	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2119	79	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	127048890	139765	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805995	139765	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	46890548	6506	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083084	6506	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44481761	5697	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	324	9	3	3	2.4	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccc1	10.1021/jm900526h
	CHEMBL1078631	5697	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	324	9	3	3	2.4	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccc1	10.1021/jm900526h
	51354301	58584	0	None	-1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688969	58584	0	None	-1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	155513612	175746	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	1204	1901	114	None	-97	10	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-97	10	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-97	10	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-97	10	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-97	10	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-97	10	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1248	1396	27	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	3077	1396	27	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	90045	1396	27	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	CHEMBL12344	1396	27	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	5310982	93179	4	None	-3	2	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	CHEMBL24665	93179	4	None	-3	2	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	1269	121	33	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	53233802	61218	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770987	61218	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	25131559	187002	0	None	23	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494667	187002	0	None	23	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	1204	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-1	10	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	44480959	5600	2	None	1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	206	5	3	3	0.1	N#C/N=C(\N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077861	5600	2	None	1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	206	5	3	3	0.1	N#C/N=C(\N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	1484519	173324	52	None	-	1	Rat	4.8	pEC50	=	4.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	227	1	1	5	0.5	CN1CCN(c2cc(Cl)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL454007	173324	52	None	-	1	Rat	4.8	pEC50	=	4.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	227	1	1	5	0.5	CN1CCN(c2cc(Cl)nc(N)n2)CC1	10.1021/jm8005959
	21638785	188073	4	None	1	2	Mouse	7.8	pEC50	=	7.8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188073	4	None	1	2	Mouse	7.8	pEC50	=	7.8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	127051958	139748	0	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805813	139748	0	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25129880	192528	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	2	1	5	2.6	CN1CCN(c2cc(-c3ccc4ccccc4c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL522841	192528	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	2	1	5	2.6	CN1CCN(c2cc(-c3ccc4ccccc4c3)nc(N)n2)CC1	10.1021/jm8005959
	25130231	187121	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	309	2	1	7	1.2	CN1CCN(c2cc(-n3cnc4ccccc43)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495280	187121	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	309	2	1	7	1.2	CN1CCN(c2cc(-n3cnc4ccccc43)nc(N)n2)CC1	10.1021/jm8005959
	25131228	187011	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL494723	187011	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25128828	187669	2	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	237	4	2	5	1.3	CN(C)CCNc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL499631	187669	2	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	237	4	2	5	1.3	CN(C)CCNc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	58227163	175889	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175889	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175889	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25071319	188474	8	None	-1	2	Mouse	7.7	pEC50	=	7.7	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188474	8	None	-1	2	Mouse	7.7	pEC50	=	7.7	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1204	1901	114	None	-1	10	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	1247	1901	114	None	-1	10	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	1375	1901	114	None	-1	10	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	774	1901	114	None	-1	10	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	CHEMBL90	1901	114	None	-1	10	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	DB05381	1901	114	None	-1	10	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	25130233	170046	6	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3cn[nH]c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL445091	170046	6	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3cn[nH]c3)nc(N)n2)CC1	10.1021/jm8005959
	25128831	172044	0	None	-1	2	Rat	4.7	pEC50	=	4.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL449157	172044	0	None	-1	2	Rat	4.7	pEC50	=	4.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	25130574	186793	0	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(N)nc(-c3ccc(C#N)cc3)c2)CC1	10.1021/jm8005959
	CHEMBL493461	186793	0	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(N)nc(-c3ccc(C#N)cc3)c2)CC1	10.1021/jm8005959
	44568790	170848	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	2	2	5	1.4	CN[C@@H]1CCN(c2nc(N)nc3c2Cc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL446269	170848	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	2	2	5	1.4	CN[C@@H]1CCN(c2nc(N)nc3c2Cc2ccccc2-3)C1	10.1021/jm800670r
	155552061	175904	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175904	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175904	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	45257133	198222	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	198222	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	11846300	79856	3	None	-2	2	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79856	3	None	-2	2	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	1204	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3032915	15321	20	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL12160	15321	20	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	53321919	56184	0	None	-50	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632409	56184	0	None	-50	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10060213	102438	1	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL306077	102438	1	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	25128823	192115	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192115	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	25131230	192507	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	329	4	1	7	1.5	COc1cccc(OC)c1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL522679	192507	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	329	4	1	7	1.5	COc1cccc(OC)c1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	10037650	5138	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106158	5138	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	170389	205253	31	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL4634086	205253	31	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL91353	205253	31	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	155513612	175746	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	136141537	56187	1	None	-1	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56187	1	None	-1	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	136192874	56189	0	None	-3	3	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56189	0	None	-3	3	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	46228133	198371	0	None	-	1	Human	6.7	pEC50	=	6.7	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595639	198371	0	None	-	1	Human	6.7	pEC50	=	6.7	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10197870	5229	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106612	5229	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10197870	5229	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106612	5229	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10879463	5218	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106570	5218	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	1278	2119	79	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2119	79	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2119	79	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2119	79	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1278	2119	79	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2119	79	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2119	79	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2119	79	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	58227260	116630	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393546	116630	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	11846636	77598	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209628	77598	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	1204	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1247	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1375	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	774	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	CHEMBL90	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	DB05381	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	16061961	197997	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	197997	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	1204	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	1247	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	1375	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	774	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	CHEMBL90	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	DB05381	1901	114	None	-1	10	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	127052635	139677	0	None	4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805003	139677	0	None	4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130237	186699	2	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	1	1	5	0.4	CN1CCN(c2cc(I)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492864	186699	2	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	1	1	5	0.4	CN1CCN(c2cc(I)nc(N)n2)CC1	10.1021/jm8005959
	127051301	139670	0	None	4	3	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139670	0	None	4	3	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25131555	186596	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3cccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492227	186596	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3cccnc3)nc(N)n2)CC1	10.1021/jm8005959
	25130914	186671	0	None	15	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL492677	186671	0	None	15	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10879463	5218	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106570	5218	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	1613	2316	44	None	-16	8	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	205	2316	44	None	-16	8	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	3964	2316	44	None	-16	8	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	CHEMBL831	2316	44	None	-16	8	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	DB00408	2316	44	None	-16	8	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	753256	178770	10	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL473662	178770	10	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1021/jm8005959
	25131560	186763	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cnn(C)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493229	186763	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cnn(C)c3)nc(N)n2)CC1	10.1021/jm8005959
	1223	940	32	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	2790	940	32	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	CHEMBL14690	940	32	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	155512725	175667	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175667	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175667	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	10037650	5138	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106158	5138	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	25129881	186599	4	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3ccncc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492241	186599	4	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3ccncc3)nc(N)n2)CC1	10.1021/jm8005959
	44568855	192591	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	285	2	1	5	2.0	CC(C)(C)c1cc(N2CCNCC2)nc(-n2cccc2)n1	10.1021/jm8005959
	CHEMBL523335	192591	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	285	2	1	5	2.0	CC(C)(C)c1cc(N2CCNCC2)nc(-n2cccc2)n1	10.1021/jm8005959
	25128832	171446	0	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	5	1.2	CN1CCN(c2cc(C(C)(C)C)nc(O)n2)CC1	10.1021/jm8005959
	CHEMBL447151	171446	0	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	5	1.2	CN1CCN(c2cc(C(C)(C)C)nc(O)n2)CC1	10.1021/jm8005959
	25071940	186771	0	None	-	1	Rat	7.6	pEC50	=	7.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	186771	0	None	-	1	Rat	7.6	pEC50	=	7.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	127049156	139664	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804837	139664	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	11437850	187078	1	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3cccc(C#N)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495082	187078	1	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3cccc(C#N)c3)nc(N)n2)CC1	10.1021/jm8005959
	25129883	192121	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ccncc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL522019	192121	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ccncc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25133972	186767	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(-c3ccc(C#N)cc3)cc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493262	186767	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(-c3ccc(C#N)cc3)cc(N)n2)CC1	10.1021/jm8005959
	25130575	187006	1	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.6	CC1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494687	187006	1	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.6	CC1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	25130577	172029	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	4	1	6	1.0	CN(C)CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL449021	172029	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	4	1	6	1.0	CN(C)CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	10773349	8007	1	None	-89	2	Human	4.6	pEC50	=	4.6	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL109130	8007	1	None	-89	2	Human	4.6	pEC50	=	4.6	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	127052634	139779	0	None	6	2	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806146	139779	0	None	6	2	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	90667240	108991	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	356	9	3	4	2.8	Cc1[nH]cnc1CCCCN/C(=N\C#N)NCCSc1ccccc1	10.1039/C3MD00245D
	CHEMBL3220642	108991	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	356	9	3	4	2.8	Cc1[nH]cnc1CCCCN/C(=N\C#N)NCCSc1ccccc1	10.1039/C3MD00245D
	25130576	187043	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187043	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25128826	188229	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.5	CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL506187	188229	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.5	CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	25130576	187043	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187043	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	58227157	115417	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354815	115417	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	1204	1901	114	None	-1	10	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1247	1901	114	None	-1	10	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1375	1901	114	None	-1	10	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	774	1901	114	None	-1	10	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	CHEMBL90	1901	114	None	-1	10	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	DB05381	1901	114	None	-1	10	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1226	1988	16	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	41376	1988	16	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	CHEMBL12608	1988	16	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	25133970	187005	2	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL494679	187005	2	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130230	171889	2	None	3	2	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL448085	171889	2	None	3	2	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	1269	121	33	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	44480958	5597	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccncc1	10.1021/jm900526h
	CHEMBL1077859	5597	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccncc1	10.1021/jm900526h
	25131556	188284	7	None	3	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL507063	188284	7	None	3	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	127051047	139781	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806162	139781	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	45257134	198261	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	CHEMBL594949	198261	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	25131231	186604	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(I)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492288	186604	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(I)cc3)nc(N)n2)CC1	10.1021/jm8005959
	10381810	203463	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL7828	203463	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	155512725	175667	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175667	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175667	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	127052294	139772	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	139772	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1901	114	None	-97	10	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-97	10	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-97	10	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-97	10	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-97	10	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-97	10	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25128822	186898	0	None	-19	2	Human	6.5	pEC50	=	6.5	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL494093	186898	0	None	-19	2	Human	6.5	pEC50	=	6.5	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	155491015	175921	3	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	175921	3	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	175921	3	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	46890072	6832	0	None	-	1	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084355	6832	0	None	-	1	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7235	0	None	3	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7235	0	None	3	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	58227373	175934	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175934	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175934	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	21638785	188073	4	None	-1	2	Rat	7.5	pEC50	=	7.5	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188073	4	None	-1	2	Rat	7.5	pEC50	=	7.5	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	25130911	184647	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	236	2	1	6	-0.1	CN1CCN(c2cc(N(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL485802	184647	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	236	2	1	6	-0.1	CN1CCN(c2cc(N(C)C)nc(N)n2)CC1	10.1021/jm8005959
	16352	116683	11	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL339414	116683	11	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	1267	3744	43	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	25130579	187129	2	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	238	4	1	5	1.3	CN(C)CCOc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL495341	187129	2	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	238	4	1	5	1.3	CN(C)CCOc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	10678661	5290	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106931	5290	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	46228101	198116	0	None	-	1	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594022	198116	0	None	-	1	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	1249	1978	14	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	13499360	1978	14	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90019	1978	14	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1269	121	33	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	25129528	186603	0	None	-	1	Rat	7.5	pEC50	=	7.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(Cl)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492287	186603	0	None	-	1	Rat	7.5	pEC50	=	7.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(Cl)c3)nc(N)n2)CC1	10.1021/jm8005959
	44568923	192501	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	327	2	1	5	2.3	CN1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL522661	192501	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	327	2	1	5	2.3	CN1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)CC1	10.1021/jm8005959
	67087450	116634	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	116634	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	44547127	115418	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115418	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227148	116631	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	116631	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	10678661	5290	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106931	5290	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	25131557	192656	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	8	0.3	COc1ncc(-c2cc(N3CCN(C)CC3)nc(N)n2)cn1	10.1021/jm8005959
	CHEMBL523835	192656	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	8	0.3	COc1ncc(-c2cc(N3CCN(C)CC3)nc(N)n2)cn1	10.1021/jm8005959
	25130909	186797	0	None	1	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	CHEMBL493478	186797	0	None	1	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	44478385	5699	2	None	20	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078642	5699	2	None	20	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	46890549	6507	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083085	6507	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44480960	5554	2	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	248	6	3	3	1.2	CC(C)N/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077636	5554	2	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	248	6	3	3	1.2	CC(C)N/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	42625303	180184	0	None	-2089	6	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180184	0	None	-2089	6	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	56954425	81669	0	None	-13	2	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	584	23	6	4	4.0	N/C(=N\C(=O)CCCCCCCCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165643	81669	0	None	-13	2	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	584	23	6	4	4.0	N/C(=N\C(=O)CCCCCCCCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	25130913	169620	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	278	2	1	7	-0.4	CN1CCN(c2cc(N3CCOCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL444484	169620	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	278	2	1	7	-0.4	CN1CCN(c2cc(N3CCOCC3)nc(N)n2)CC1	10.1021/jm8005959
	1274	4001	12	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	4001	12	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	4001	12	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	155517880	176093	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176093	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176093	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	175904	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175904	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175904	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	25129180	186668	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	4	1	6	0.7	CCN(CC)c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL492662	186668	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	4	1	6	0.7	CCN(CC)c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	3228743	186798	5	None	-	1	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL493479	186798	5	None	-	1	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	12769844	108990	0	None	-3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	234	5	3	3	0.7	CN/C(=N/C#N)NCCCCc1nc[nH]c1C	10.1039/C3MD00245D
	CHEMBL3220641	108990	0	None	-3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	234	5	3	3	0.7	CN/C(=N/C#N)NCCCCc1nc[nH]c1C	10.1039/C3MD00245D
	25129181	186669	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	305	3	1	7	-0.0	CN1CCN(c2cc(N3CCC(N(C)C)C3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492663	186669	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	305	3	1	7	-0.0	CN1CCN(c2cc(N3CCC(N(C)C)C3)nc(N)n2)CC1	10.1021/jm8005959
	25128825	186981	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.2	CN1CCC(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494585	186981	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.2	CN1CCC(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	44478385	5699	2	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/acs.jmedchem.6b00120
	CHEMBL1078642	5699	2	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/acs.jmedchem.6b00120
	44478385	5699	2	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1039/C3MD00245D
	CHEMBL1078642	5699	2	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1039/C3MD00245D
	25129527	186831	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493720	186831	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm8005959
	24798596	198185	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594491	198185	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	127052292	139736	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805663	139736	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1274	4001	12	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	4001	12	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	4001	12	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	11846465	141400	0	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	CHEMBL387054	141400	0	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	25131559	187002	0	None	-23	2	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494667	187002	0	None	-23	2	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	25131561	186821	0	None	3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493664	186821	0	None	3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	46235428	6526	0	None	-263	3	Mouse	7.4	pEC50	=	7.4	Functional	Intrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6526	0	None	-263	3	Mouse	7.4	pEC50	=	7.4	Functional	Intrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1269	121	33	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	136030655	56188	0	None	-3	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56188	0	None	-3	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25129523	186704	40	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492884	186704	40	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25129523	186704	40	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	186704	40	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	70692530	75339	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL2047474	75339	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	91613	17804	31	None	-5	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL12620	17804	31	None	-5	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	58227326	116626	0	None	-	1	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393542	116626	0	None	-	1	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	58227373	175934	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175934	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175934	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	25071004	192116	0	None	1	2	Mouse	8.4	pEC50	=	8.4	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192116	0	None	1	2	Mouse	8.4	pEC50	=	8.4	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	42625303	180184	0	None	-2	6	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180184	0	None	-2	6	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	25128824	192575	0	None	1	2	Mouse	8.3	pEC50	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192575	0	None	1	2	Mouse	8.3	pEC50	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	10702196	108081	1	None	-1	2	Human	4.4	pEC50	=	4.4	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL320279	108081	1	None	-1	2	Human	4.4	pEC50	=	4.4	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	56954320	81667	0	None	-10	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	472	15	6	4	0.9	N/C(=N\C(=O)CCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165641	81667	0	None	-10	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	472	15	6	4	0.9	N/C(=N\C(=O)CCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	1267	3744	43	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	3035905	3744	43	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	CHEMBL260374	3744	43	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	25129524	186601	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	CHEMBL492274	186601	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	25130909	186797	0	None	-1	2	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	CHEMBL493478	186797	0	None	-1	2	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	1278	2119	79	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2119	79	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2119	79	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2119	79	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	25128833	187818	30	None	2	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	CHEMBL501882	187818	30	None	2	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	25129526	186830	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL493719	186830	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	24797248	198184	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594490	198184	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	127050434	139720	0	None	3	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	160	3	4	3	0.2	CC(=N)SCCNC(=N)N	10.1021/acs.jmedchem.6b00120
	CHEMBL3805514	139720	0	None	3	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	160	3	4	3	0.2	CC(=N)SCCNC(=N)N	10.1021/acs.jmedchem.6b00120
	25129169	186598	0	None	1	2	Mouse	7.3	pEC50	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186598	0	None	1	2	Mouse	7.3	pEC50	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	24798595	169808	2	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	276	2	1	6	0.8	CN1CCN(c2cc(N3CCCCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL444782	169808	2	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	276	2	1	6	0.8	CN1CCN(c2cc(N3CCCCC3)nc(N)n2)CC1	10.1021/jm8005959
	90667239	108989	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	8	3	4	2.5	Cc1nc(CCCN/C(=N\C#N)NCCSc2ccccc2)c[nH]1	10.1039/C3MD00245D
	CHEMBL3220640	108989	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	8	3	4	2.5	Cc1nc(CCCN/C(=N\C#N)NCCSc2ccccc2)c[nH]1	10.1039/C3MD00245D
	25130573	186302	2	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL489663	186302	2	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130581	186642	0	None	-3	2	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL492489	186642	0	None	-3	2	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	25130235	187004	1	None	-4	3	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187004	1	None	-4	3	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	46890546	6437	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6437	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228143	198400	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595880	198400	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	117724523	116613	2	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393529	116613	2	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	25130914	186671	0	None	-15	2	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492677	186671	0	None	-15	2	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25129179	187085	4	None	-	1	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	222	2	2	6	-0.1	CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL495112	187085	4	None	-	1	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	222	2	2	6	-0.1	CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25130236	187118	0	None	1	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	CHEMBL495265	187118	0	None	1	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	25068753	187854	15	None	1	2	Mouse	8.2	pEC50	=	8.2	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187854	15	None	1	2	Mouse	8.2	pEC50	=	8.2	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	53233805	61195	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770964	61195	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	46890545	7304	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086460	7304	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1274	4001	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	3063228	4001	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	CHEMBL1196470	4001	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	1274	4001	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	3063228	4001	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	CHEMBL1196470	4001	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	136141537	56187	1	None	-5	3	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56187	1	None	-5	3	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	11313837	2464	7	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1254	2464	7	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL175782	2464	7	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	10583206	108872	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321920	108872	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	1204	1901	114	None	-51	10	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-51	10	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-51	10	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-51	10	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-51	10	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-51	10	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	155513612	175746	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155512725	175667	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175667	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175667	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	58227163	175889	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175889	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175889	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25130580	173730	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.6	CNc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL455016	173730	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.6	CNc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	25129878	186752	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	324	3	1	7	1.4	COc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL493103	186752	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	324	3	1	7	1.4	COc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	127052295	139667	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	352	7	3	4	2.4	N#C/N=C(/NCCOc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804862	139667	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	352	7	3	4	2.4	N#C/N=C(/NCCOc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1901	114	None	-97	10	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-97	10	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-97	10	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-97	10	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-97	10	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-97	10	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	53233803	61193	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770962	61193	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	25129169	186598	0	None	-1	2	Rat	7.3	pEC50	=	7.3	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186598	0	None	-1	2	Rat	7.3	pEC50	=	7.3	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	127049856	139715	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805431	139715	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	90667238	108988	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	220	4	3	3	0.3	CN/C(=N/C#N)NCCCc1c[nH]c(C)n1	10.1039/C3MD00245D
	CHEMBL3220639	108988	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	220	4	3	3	0.3	CN/C(=N/C#N)NCCCc1c[nH]c(C)n1	10.1039/C3MD00245D
	565544	109255	9	None	-4466	2	Human	6.3	pEC50	=	6.3	Functional	Inhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptor	ChEMBL	125	2	2	2	0.5	CC(CN)c1cnc[nH]1	10.1021/jm0300025
	CHEMBL322988	109255	9	None	-4466	2	Human	6.3	pEC50	=	6.3	Functional	Inhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptor	ChEMBL	125	2	2	2	0.5	CC(CN)c1cnc[nH]1	10.1021/jm0300025
	10583206	108872	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321920	108872	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	25130915	186705	0	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL492885	186705	0	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	46228125	198305	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595215	198305	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	44482028	5548	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccn1	10.1021/jm900526h
	CHEMBL1077580	5548	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccn1	10.1021/jm900526h
	25131232	186707	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	308	2	1	6	1.7	Cc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL492900	186707	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	308	2	1	6	1.7	Cc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	25130235	187004	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187004	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187004	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187004	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	45257133	198222	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	198222	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25128824	192575	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192575	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	155517880	176093	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176093	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176093	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	45269820	193679	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202326	193679	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL551790	193679	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	25071004	192116	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192116	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	46890022	6968	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	8	3	4	4.3	Cc1cc(OCCCNCC2CCCNC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084869	6968	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	8	3	4	4.3	Cc1cc(OCCCNCC2CCCNC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228158	200472	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL609289	200472	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890547	6438	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082772	6438	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	1255	3146	11	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	6421522	3146	11	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL19173	3146	11	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	9974306	202769	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72372	202769	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	10060212	202829	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72769	202829	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	136192874	56189	0	None	1	3	Mouse	7.2	pEC50	=	7.2	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56189	0	None	1	3	Mouse	7.2	pEC50	=	7.2	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1236	2036	23	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1237	2036	23	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	156615	2036	23	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL268229	2036	23	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	25129175	186796	0	None	-	1	Rat	6.2	pEC50	=	6.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL493477	186796	0	None	-	1	Rat	6.2	pEC50	=	6.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	25130914	186671	0	None	15	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492677	186671	0	None	15	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25130908	171285	2	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL446896	171285	2	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	24797775	186662	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492631	186662	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	1204	1901	114	None	-51	10	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-51	10	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-51	10	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-51	10	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-51	10	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-51	10	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25130912	188153	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL505061	188153	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1021/jm8005959
	1204	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25149521	178474	0	None	9	2	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL471413	178474	0	None	9	2	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1247	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1375	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	774	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	CHEMBL90	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	DB05381	1901	114	None	-1	10	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1267	3744	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3744	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3744	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	1269	121	33	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	37463	121	33	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	CHEMBL275443	121	33	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	1267	3744	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3744	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3744	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	44482031	5566	1	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	234	6	3	3	0.8	CCN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077756	5566	1	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	234	6	3	3	0.8	CCN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	46890019	7231	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7231	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	156015638	177697	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177697	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177697	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	1279	2119	79	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	4908365	2119	79	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	CHEMBL129198	2119	79	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	10978141	5081	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL105803	5081	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	58227127	116627	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393543	116627	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	44481489	5637	0	None	18	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(\NCCCc1ccccc1)NCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1078210	5637	0	None	18	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(\NCCCc1ccccc1)NCCCc1c[nH]cn1	10.1021/jm900526h
	10419556	5286	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106913	5286	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	11086114	7313	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL108649	7313	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10820930	5722	0	None	-	1	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL107877	5722	0	None	-	1	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10106063	108783	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321860	108783	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10106063	108783	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	CHEMBL321860	108783	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	56954318	81666	0	None	2	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	444	13	6	4	0.1	N/C(=N\C(=O)CCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165640	81666	0	None	2	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	444	13	6	4	0.1	N/C(=N\C(=O)CCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	10106063	108783	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321860	108783	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10017000	202780	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72431	202780	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1901	114	None	-1	10	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-1	10	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-1	10	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-1	10	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-1	10	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-1	10	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	136192874	56189	0	None	-1	3	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56189	0	None	-1	3	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1267	3744	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	3035905	3744	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	CHEMBL260374	3744	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	1239	2002	23	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1240	2002	23	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	912	2002	23	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL268490	2002	23	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10978141	5081	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL105803	5081	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	1278	2119	79	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	1279	2119	79	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	4908365	2119	79	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	CHEMBL129198	2119	79	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	42625303	180184	0	None	-501	6	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180184	0	None	-501	6	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	10419556	5286	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106913	5286	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	11086114	7313	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL108649	7313	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	155513612	175746	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	25130230	171889	2	None	-3	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL448085	171889	2	None	-3	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	25133971	186766	2	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2nc(N)cc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL493261	186766	2	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2nc(N)cc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130229	188169	6	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	271	2	1	7	0.3	CN1CCN(c2cc(-c3cncnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL505346	188169	6	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	271	2	1	7	0.3	CN1CCN(c2cc(-c3cncnc3)nc(N)n2)CC1	10.1021/jm8005959
	56954515	81653	0	None	-2	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	546	17	6	6	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165628	81653	0	None	-2	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	546	17	6	6	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm201128q
	56954322	81668	0	None	-4	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	500	17	6	4	1.7	N/C(=N\C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165642	81668	0	None	-4	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	500	17	6	4	1.7	N/C(=N\C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	45257131	198226	0	None	-	1	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	198226	0	None	-	1	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	11846298	138751	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	CHEMBL379102	138751	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	25129176	172192	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2nc(N)ncc2-c2ccccc2)CC1	10.1021/jm8005959
	CHEMBL451108	172192	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2nc(N)ncc2-c2ccccc2)CC1	10.1021/jm8005959
	58227388	116622	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393538	116622	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	1223	940	32	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	2790	940	32	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	CHEMBL14690	940	32	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	10820929	107721	1	None	-30	2	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL319658	107721	1	None	-30	2	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	53233801	61216	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770985	61216	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	58227252	116628	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393544	116628	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	1204	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-51	10	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1274	4001	12	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	4001	12	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	4001	12	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	25130236	187118	0	None	-1	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	CHEMBL495265	187118	0	None	-1	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	1267	3744	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3744	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3744	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	25129877	168893	0	None	-	1	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(I)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL442865	168893	0	None	-	1	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(I)c3)nc(N)n2)CC1	10.1021/jm8005959
	44568847	186666	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	6	2.0	CN1CCN(c2cc(Sc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492652	186666	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	6	2.0	CN1CCN(c2cc(Sc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25101945	192504	0	None	1	2	Mouse	8.0	pEC50	=	8.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192504	0	None	1	2	Mouse	8.0	pEC50	=	8.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	25101945	192504	0	None	-1	2	Rat	8.0	pEC50	=	8.0	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192504	0	None	-1	2	Rat	8.0	pEC50	=	8.0	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	58227143	116625	1	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393541	116625	1	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	16061961	197997	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	197997	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	112936251	136742	1	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752085	136742	1	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	58227163	175889	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175889	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175889	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25131556	188284	7	None	-3	2	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL507063	188284	7	None	-3	2	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25128830	187130	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	234	1	0	4	1.5	CN1CCN(c2cc(C(C)(C)C)ncn2)CC1	10.1021/jm8005959
	CHEMBL495342	187130	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	234	1	0	4	1.5	CN1CCN(c2cc(C(C)(C)C)ncn2)CC1	10.1021/jm8005959
	127035835	136761	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752270	136761	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	11983344	5601	5	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	220	5	3	3	0.4	CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077862	5601	5	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	220	5	3	3	0.4	CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	25128829	186751	0	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	265	5	1	5	1.7	CN(C)CCCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL493100	186751	0	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	265	5	1	5	1.7	CN(C)CCCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	24745335	292	7	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	156013896	177665	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	44129134	197827	0	None	-	1	Human	8.0	pIC50	=	8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	197827	0	None	-	1	Human	8.0	pIC50	=	8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	56835171	69397	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69397	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	16082828	79850	0	None	-6	3	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213886	79850	0	None	-6	3	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	71525678	89295	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89295	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	71525679	89296	0	None	-	1	Human	6.9	pIC50	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89296	0	None	-	1	Human	6.9	pIC50	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	1278	2119	79	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	1279	2119	79	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	4908365	2119	79	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	CHEMBL129198	2119	79	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	10446295	2101	29	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2101	29	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2101	29	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	155563181	174731	0	None	-	1	Human	5.9	pIC50	=	5.9	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	498	12	1	7	5.1	CCOC(=O)C1=C(C)NC(C)=C(C(=O)OCC)C1c1ccc(OCCCCCN2CCCCC2)cc1	10.1021/acs.jmedchem.9b00937
	CHEMBL4573309	174731	0	None	-	1	Human	5.9	pIC50	=	5.9	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	498	12	1	7	5.1	CCOC(=O)C1=C(C)NC(C)=C(C(=O)OCC)C1c1ccc(OCCCCCN2CCCCC2)cc1	10.1021/acs.jmedchem.9b00937
	25147771	173666	0	None	-	1	Human	6.8	pIC50	=	6.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	173666	0	None	-	1	Human	6.8	pIC50	=	6.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	16082833	79937	0	None	-40	2	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214312	79937	0	None	-40	2	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	90645145	111686	0	None	-	1	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290578	111686	0	None	-	1	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1267	3744	43	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	3035905	3744	43	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	CHEMBL260374	3744	43	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	1278	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1278	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	1279	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	4908365	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	CHEMBL129198	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	3201696	15593	3	None	-22	2	Human	4.7	pIC50	=	4.7	Functional	Antagonist activity at human histamine H4 receptorAntagonist activity at human histamine H4 receptor	ChEMBL	348	5	0	6	2.1	CN1CCN(C(c2ccccc2)c2nnnn2Cc2ccccc2)CC1	10.1016/j.bmcl.2010.07.009
	CHEMBL1222728	15593	3	None	-22	2	Human	4.7	pIC50	=	4.7	Functional	Antagonist activity at human histamine H4 receptorAntagonist activity at human histamine H4 receptor	ChEMBL	348	5	0	6	2.1	CN1CCN(C(c2ccccc2)c2nnnn2Cc2ccccc2)CC1	10.1016/j.bmcl.2010.07.009
	1278	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	63868328	173679	2	None	-	1	Human	6.6	pIC50	=	6.6	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4549015	173679	2	None	-	1	Human	6.6	pIC50	=	6.6	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71548412	1878	14	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1878	14	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1878	14	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	16656871	7894	0	None	-	1	Human	6.5	pIC50	=	6.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090531	7894	0	None	-	1	Human	6.5	pIC50	=	6.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	16718567	8078	0	None	3311	2	Human	8.5	pIC50	=	8.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091874	8078	0	None	3311	2	Human	8.5	pIC50	=	8.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2119	79	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	155540438	171974	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4483783	171974	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	44129130	197875	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	197875	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11682208	8190	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092542	8190	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	25112105	8079	0	None	-	1	Human	8.4	pIC50	=	8.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091875	8079	0	None	-	1	Human	8.4	pIC50	=	8.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	24745335	292	7	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	1278	2119	79	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2119	79	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2119	79	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2119	79	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	16656872	7830	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090190	7830	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	122193095	123396	0	None	-	1	Human	6.4	pIC50	=	6.4	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123396	0	None	-	1	Human	6.4	pIC50	=	6.4	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	49841846	69395	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69395	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	49841719	69394	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69394	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	1278	2119	79	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	90645147	111684	0	None	-	1	Human	6.3	pIC50	=	6.3	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290576	111684	0	None	-	1	Human	6.3	pIC50	=	6.3	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	24745335	292	7	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	10446295	2101	29	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2101	29	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2101	29	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	122194625	123503	0	None	-	1	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629828	123503	0	None	-	1	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	1267	3744	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	3035905	3744	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	CHEMBL260374	3744	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	1267	3744	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3035905	3744	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	CHEMBL260374	3744	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	25147772	172837	4	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	172837	4	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	71548412	1878	14	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1878	14	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1878	14	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	11709443	8162	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092332	8162	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71519674	89299	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89299	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	16656874	8022	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091569	8022	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	49841845	69396	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69396	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	156013896	177665	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2119	79	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2119	79	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2119	79	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	16082831	79936	0	None	-100	3	Human	6.1	pIC50	=	6.1	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL214311	79936	0	None	-100	3	Human	6.1	pIC50	=	6.1	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	71525681	89298	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89298	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	1267	3744	43	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3744	43	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3744	43	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	58227148	116631	0	None	-	1	Human	10.1	pKd	=	10.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	116631	0	None	-	1	Human	10.1	pKd	=	10.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	59777258	109644	0	None	-	0	Human	10.0	pKd	=	10	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236561	109644	0	None	-	0	Human	10.0	pKd	=	10	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	24797604	109635	2	None	-	0	Human	9.9	pKd	=	9.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	CHEMBL3236552	109635	2	None	-	0	Human	9.9	pKd	=	9.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	24994542	109637	0	None	-	0	Human	9.6	pKd	=	9.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236554	109637	0	None	-	0	Human	9.6	pKd	=	9.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	24798144	109636	2	None	-	1	Human	9.5	pKd	=	9.5	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109636	2	None	-	1	Human	9.5	pKd	=	9.5	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	53257796	109658	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236575	109658	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	54587790	61211	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770980	61211	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	24745337	61210	0	None	-	0	Human	9.3	pKd	=	9.3	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770979	61210	0	None	-	0	Human	9.3	pKd	=	9.3	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	59777338	109639	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236556	109639	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	44547268	116610	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393526	116610	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227340	116616	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393532	116616	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	58227200	116619	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393535	116619	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	54585781	61232	0	None	-	0	Human	8.9	pKd	=	8.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771000	61232	0	None	-	0	Human	8.9	pKd	=	8.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	54583873	61200	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770969	61200	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54587787	61202	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770971	61202	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54581940	61223	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770992	61223	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25130912	188153	0	None	-	1	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL505061	188153	0	None	-	1	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	23653543	67706	2	None	-	0	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914541	67706	2	None	-	0	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	24994636	109648	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	CHEMBL3236565	109648	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	42624149	109651	1	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	109651	1	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	59777262	109655	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236572	109655	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	54584824	61208	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770977	61208	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227337	116637	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393553	116637	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	58227259	116640	0	None	-	1	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	116640	0	None	-	1	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	24798144	109636	2	None	-	1	Mouse	8.0	pKd	=	8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109636	2	None	-	1	Mouse	8.0	pKd	=	8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	24826766	67759	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914750	67759	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	9944571	116516	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338937	116516	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	24994634	2111	26	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2111	26	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2111	26	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	53258370	109652	1	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236569	109652	1	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	24994635	109653	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236570	109653	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	54582865	61190	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770959	61190	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	54584825	61221	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770990	61221	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	11587279	124531	3	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL364400	124531	3	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	46890627	6511	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083098	6511	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740470	109310	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3233048	109310	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	11687917	14796	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	CHEMBL120854	14796	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	9966927	18687	4	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128252	18687	4	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	54581938	61203	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770972	61203	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	67087450	116634	0	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	116634	0	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	11594278	14521	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL120591	14521	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	9923162	18751	5	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128613	18751	5	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	11120826	18730	1	None	-	0	Human	6.8	pKd	=	6.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128505	18730	1	None	-	0	Human	6.8	pKd	=	6.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	46890677	7210	0	None	-	0	Human	6.8	pKd	=	6.8	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086038	7210	0	None	-	0	Human	6.8	pKd	=	6.8	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7235	0	None	-3	4	Mouse	4.8	pKd	=	4.8	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7235	0	None	-3	4	Mouse	4.8	pKd	=	4.8	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740898	109645	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	CHEMBL3236562	109645	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	10468601	193918	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202324	193918	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL555434	193918	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	53233904	61196	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770965	61196	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	10446295	2101	29	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2101	29	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2101	29	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	4242975	18654	9	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128088	18654	9	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	9900313	19226	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129444	19226	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	9839217	72174	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199062	72174	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	11565330	96771	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	CHEMBL268525	96771	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	4906599	168097	10	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL436381	168097	10	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	24996854	109647	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	CHEMBL3236564	109647	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	42624150	109660	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236577	109660	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	24797956	109641	3	None	-	1	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	109641	3	None	-	1	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	24992735	109666	0	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	CHEMBL3236582	109666	0	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	10244243	140782	3	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL383384	140782	3	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	46890592	6315	0	None	-	2	Human	7.6	pKd	=	7.6	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6315	0	None	-	2	Human	7.6	pKd	=	7.6	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44547127	115418	0	None	-	1	Human	8.5	pKd	=	8.5	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115418	0	None	-	1	Human	8.5	pKd	=	8.5	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	24992077	109654	0	None	-	0	Human	8.4	pKd	=	8.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236571	109654	0	None	-	0	Human	8.4	pKd	=	8.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	10934567	19062	1	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL129026	19062	1	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	11630326	72226	0	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199231	72226	0	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	44547567	116617	2	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393533	116617	2	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	4060730	19183	51	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129199	19183	51	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	53378711	109661	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236578	109661	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	24994634	2111	26	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2111	26	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2111	26	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	54753437	67841	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915048	67841	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	42624149	109651	1	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	109651	1	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	46890590	6946	0	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6946	0	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11572877	66745	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL187285	66745	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	9992865	113928	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL333220	113928	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	11601630	132296	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL370135	132296	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	46890546	6437	0	None	-	1	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6437	0	None	-	1	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890590	6946	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6946	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	2063135	8293	9	None	-	1	Human	8.2	pKd	=	8.2	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1093180	8293	9	None	-	1	Human	8.2	pKd	=	8.2	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	11066922	19225	1	None	-	0	Human	8.2	pKd	=	8.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129436	19225	1	None	-	0	Human	8.2	pKd	=	8.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	24994634	2111	26	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2111	26	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2111	26	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	10083188	140403	3	None	-	0	Human	7.2	pKd	=	7.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL382276	140403	3	None	-	0	Human	7.2	pKd	=	7.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	46890019	7231	0	None	-	1	Human	7.2	pKd	=	7.2	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7231	0	None	-	1	Human	7.2	pKd	=	7.2	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1278	2119	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2119	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2119	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2119	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	10967174	19242	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19242	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	42624148	109657	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236574	109657	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	54583875	61226	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770995	61226	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227179	116632	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393548	116632	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	58227086	116641	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393557	116641	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2119	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2119	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2119	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2119	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	10967174	19242	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129540	19242	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	10988959	117605	4	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL340740	117605	4	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	10923241	18972	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128958	18972	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	9878733	116344	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338051	116344	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	11623296	116522	0	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338972	116522	0	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	16202258	67876	0	None	-	0	Human	10.5	pKi	=	10.5	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915536	67876	0	None	-	0	Human	10.5	pKi	=	10.5	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	24745506	67877	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915537	67877	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	44187557	193827	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	CHEMBL1202332	193827	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	CHEMBL553423	193827	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	135525668	67870	0	None	-	0	Human	8.0	pKi	=	8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915348	67870	0	None	-	0	Human	8.0	pKi	=	8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	136042270	69572	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938979	69572	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24745507	67878	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915538	67878	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	57401167	69526	0	None	-	0	Human	6.9	pKi	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938846	69526	0	None	-	0	Human	6.9	pKi	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400778	67868	0	None	-	0	Human	6.8	pKi	=	6.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915346	67868	0	None	-	0	Human	6.8	pKi	=	6.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	135525668	67870	0	None	-	0	Human	7.8	pKi	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915348	67870	0	None	-	0	Human	7.8	pKi	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57391926	69559	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938967	69559	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042269	69571	0	None	-	0	Human	7.7	pKi	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938978	69571	0	None	-	0	Human	7.7	pKi	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57402929	69524	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938844	69524	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57395538	67869	0	None	-	0	Human	6.6	pKi	=	6.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915347	67869	0	None	-	0	Human	6.6	pKi	=	6.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042267	69569	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938976	69569	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400777	67867	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915345	67867	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	57400777	67867	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915345	67867	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	49841846	69395	0	None	-	1	Human	8.5	pKi	=	8.5	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69395	0	None	-	1	Human	8.5	pKi	=	8.5	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	11688307	69560	0	None	-	0	Human	5.5	pKi	=	5.5	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938968	69560	0	None	-	0	Human	5.5	pKi	=	5.5	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	54754351	67875	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915535	67875	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	57400778	67868	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915346	67868	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	10446295	2101	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	1277	2101	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL185951	2101	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	10446295	2101	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	1277	2101	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL185951	2101	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	49841719	69394	0	None	-	1	Human	8.4	pKi	=	8.4	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69394	0	None	-	1	Human	8.4	pKi	=	8.4	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	57391927	69561	0	None	-	0	Human	5.4	pKi	=	5.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938969	69561	0	None	-	0	Human	5.4	pKi	=	5.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1278	2119	79	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2119	79	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2119	79	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2119	79	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	49841845	69396	0	None	-	1	Human	8.3	pKi	=	8.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69396	0	None	-	1	Human	8.3	pKi	=	8.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	57395538	67869	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915347	67869	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	136042265	69567	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938974	69567	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57395620	67874	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915534	67874	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	57395620	67874	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915534	67874	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	1278	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1278	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1279	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	4908365	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL129198	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1278	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2119	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1267	3744	43	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3744	43	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3744	43	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	56835171	69397	0	None	-	1	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69397	0	None	-	1	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	57390145	69563	0	None	-	0	Human	5.1	pKi	=	5.1	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938970	69563	0	None	-	0	Human	5.1	pKi	=	5.1	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	136042268	69570	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938977	69570	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042266	69568	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938975	69568	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	54754351	67875	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915535	67875	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	56950378	69525	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938845	69525	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	135	2496	38	None	-2	30	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1796	2496	38	None	-2	30	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	4184	2496	38	None	-2	30	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	CHEMBL6437	2496	38	None	-2	30	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	DB06148	2496	38	None	-2	30	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1225	1443	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	3958	1443	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	667477	1443	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	CHEMBL860	1443	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	DB01142	1443	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	5344	172901	98	None	60	5	Human	8.3	pIC50	=	8.3	Functional	NoneNone	Drug Central	267	3	2	5	1.7	Cc1noc(NS(=O)(=O)c2ccc(N)cc2)c1C	None
	CHEMBL453	172901	98	None	60	5	Human	8.3	pIC50	=	8.3	Functional	NoneNone	Drug Central	267	3	2	5	1.7	Cc1noc(NS(=O)(=O)c2ccc(N)cc2)c1C	None
	1272	818	0	None	-	1	Human	8.1	pIC50	None	8.1	Functional	UnclassifiedUnclassified	Guide to Pharmacology		None	None	None		None	15265943

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Ligands					Receptor			Assay information							Chemical information
Sel. page	Common name	GPCRdb ID	#Vendors	Reference ligand	Fold selectivity (Affinity)	# tested GPCRs (Affinity)	Species	p-value (-log)	Type	Activity Relation	Activity Value	Assay Type	Assay Description	Source	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles	DOI
	51354301	58584	0	None	-10	2	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	CHEMBL1688969	58584	0	None	-10	2	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	70692530	75339	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL2047474	75339	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	90667434	109001	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	2	3	1.9	CC(CC(=O)/N=C(\N)NCCCn1ccnc1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL3220892	109001	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	2	3	1.9	CC(CC(=O)/N=C(\N)NCCCn1ccnc1)c1ccccc1	10.1039/C3MD00245D
	44367568	45484	5	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@@H]2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153051	45484	5	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@@H]2CCNC2)c[nH]1	10.1021/jm030905y
	9877454	203901	1	None	-4	2	Human	5.0	pEC50	=	5.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL81644	203901	1	None	-4	2	Human	5.0	pEC50	=	5.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	155512725	175667	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175667	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175667	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	1267	3744	43	None	-33	25	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	3035905	3744	43	None	-33	25	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	CHEMBL260374	3744	43	None	-33	25	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	9793791	45759	3	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	137	2	2	2	0.5	NCC1CC1c1c[nH]cn1	10.1021/jm030905y
	CHEMBL153284	45759	3	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	137	2	2	2	0.5	NCC1CC1c1c[nH]cn1	10.1021/jm030905y
	155524115	175973	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175973	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175973	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	1204	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1247	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1375	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	774	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	DB05381	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1236	2036	23	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	1237	2036	23	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	156615	2036	23	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	CHEMBL268229	2036	23	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	46890592	6315	0	None	-	1	Mouse	5.9	pEC50	=	5.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6315	0	None	-	1	Mouse	5.9	pEC50	=	5.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7235	0	None	-4786	2	Mouse	7.9	pEC50	=	7.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7235	0	None	-4786	2	Mouse	7.9	pEC50	=	7.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1204	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1247	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1375	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	774	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	DB05381	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1204	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1247	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1375	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	774	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	DB05381	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1278	2119	79	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	1279	2119	79	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	4908365	2119	79	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	CHEMBL129198	2119	79	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	155524115	175973	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	175973	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	175973	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	11344458	203487	2	None	-14	2	Human	5.9	pEC50	=	5.9	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78497	203487	2	None	-14	2	Human	5.9	pEC50	=	5.9	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	1204	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1901	114	None	-3	23	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	24797956	109641	3	None	-	1	Human	5.9	pEC50	=	5.9	Binding	Agonist activity at human H4 receptorAgonist activity at human H4 receptor	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	109641	3	None	-	1	Human	5.9	pEC50	=	5.9	Binding	Agonist activity at human H4 receptorAgonist activity at human H4 receptor	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	58227373	175934	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175934	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175934	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	175904	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175904	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175904	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	46887115	8426	0	None	-	0	Human	5.9	pEC50	=	5.9	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)C[C@H](C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1094144	8426	0	None	-	0	Human	5.9	pEC50	=	5.9	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)C[C@H](C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	58227373	175934	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175934	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175934	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155517880	176093	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176093	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176093	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	25149335	183643	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm9000693
	CHEMBL483406	183643	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm9000693
	25149335	183643	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL483406	183643	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1039/C3MD00245D
	25149335	183643	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm800841w
	CHEMBL483406	183643	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm800841w
	45267230	194808	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202318	194808	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL563342	194808	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	42625223	179345	0	None	-	1	Human	6.8	pEC50	=	6.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	395	8	4	3	1.4	CN(CCC(=O)/N=C(\N)NCCCc1c[nH]cn1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm9000693
	CHEMBL474617	179345	0	None	-	1	Human	6.8	pEC50	=	6.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	395	8	4	3	1.4	CN(CCC(=O)/N=C(\N)NCCCc1c[nH]cn1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm9000693
	11240759	104734	5	None	-190	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL311473	104734	5	None	-190	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	9812843	203533	1	None	-4	2	Human	4.8	pEC50	=	4.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78838	203533	1	None	-4	2	Human	4.8	pEC50	=	4.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	11469514	103691	2	None	-64	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL309654	103691	2	None	-64	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	155524115	175973	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175973	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175973	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	127052293	139708	1	None	-	4	Mouse	6.8	pEC50	=	6.8	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139708	1	None	-	4	Mouse	6.8	pEC50	=	6.8	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	155513612	175746	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42626109	179188	0	None	-	1	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	324	6	4	2	1.5	N/C(=N\C(=O)Cc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL474447	179188	0	None	-	1	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	324	6	4	2	1.5	N/C(=N\C(=O)Cc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	155512725	175667	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175667	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175667	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	42625386	177778	0	None	-	1	Human	8.6	pEC50	=	8.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	6	3	2	0.6	CCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL465170	177778	0	None	-	1	Human	8.6	pEC50	=	8.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	6	3	2	0.6	CCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	46830167	8673	0	None	-	0	Human	8.6	pEC50	=	8.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096429	8673	0	None	-	0	Human	8.6	pEC50	=	8.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	11171320	45669	5	None	-	1	Human	5.7	pEC50	=	5.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	179	3	2	2	1.3	c1nc(CCC2CCCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153207	45669	5	None	-	1	Human	5.7	pEC50	=	5.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	179	3	2	2	1.3	c1nc(CCC2CCCNC2)c[nH]1	10.1021/jm030905y
	170389	205253	31	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	CHEMBL4634086	205253	31	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	CHEMBL91353	205253	31	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	46887041	8672	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096428	8672	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	155517880	176093	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176093	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176093	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	1204	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-15	23	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	127052293	139708	1	None	34	4	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139708	1	None	34	4	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11301441	95707	6	None	-	1	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL260549	95707	6	None	-	1	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/acs.jmedchem.8b00435
	1278	2119	79	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	1279	2119	79	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	4908365	2119	79	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	CHEMBL129198	2119	79	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	58227401	175877	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175877	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175877	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42625222	179190	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	367	7	5	3	0.7	N/C(=N\C(=O)CNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL474449	179190	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	367	7	5	3	0.7	N/C(=N\C(=O)CNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	1267	3744	43	None	-21	25	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-21	25	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-21	25	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	90667228	108981	0	None	-	0	Human	5.5	pEC50	=	5.5	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL3220629	108981	0	None	-	0	Human	5.5	pEC50	=	5.5	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1ccccc1	10.1039/C3MD00245D
	58227401	175877	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175877	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175877	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42625303	180184	0	None	-	1	Human	8.5	pEC50	=	8.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL475621	180184	0	None	-	1	Human	8.5	pEC50	=	8.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	42625304	178522	0	None	-	1	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	5	3	2	0.4	CC(C)C(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL471724	178522	0	None	-	1	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	5	3	2	0.4	CC(C)C(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	11286909	203573	2	None	-1	2	Human	5.5	pEC50	=	5.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL79126	203573	2	None	-1	2	Human	5.5	pEC50	=	5.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	9964223	203974	11	None	-36	2	Human	4.5	pEC50	=	4.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL82244	203974	11	None	-36	2	Human	4.5	pEC50	=	4.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	42626111	189102	0	None	-	0	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	381	8	5	3	1.1	N/C(=N\C(=O)CCNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL515428	189102	0	None	-	0	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	381	8	5	3	1.1	N/C(=N\C(=O)CCNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	1204	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-3	23	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	58227373	175934	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175934	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175934	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	175904	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175904	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175904	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	58227163	175889	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175889	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175889	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	127050776	139717	0	None	-	2	Mouse	5.4	pEC50	=	5.4	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	139717	0	None	-	2	Mouse	5.4	pEC50	=	5.4	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1901	114	None	-12	23	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-12	23	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-12	23	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-12	23	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-12	23	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-12	23	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	45269820	193679	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202326	193679	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL551790	193679	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	155512725	175667	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175667	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175667	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	16078981	43823	12	None	-	1	Human	5.4	pEC50	=	5.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	c1nc(CC2CCCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL151467	43823	12	None	-	1	Human	5.4	pEC50	=	5.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	c1nc(CC2CCCNC2)c[nH]1	10.1021/jm030905y
	155524115	175973	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175973	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175973	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	46887043	8675	0	None	-	0	Human	8.3	pEC50	=	8.3	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096431	8675	0	None	-	0	Human	8.3	pEC50	=	8.3	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	42625384	169206	0	None	-	1	Human	8.3	pEC50	=	8.3	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	209	4	3	2	-0.2	CC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL443896	169206	0	None	-	1	Human	8.3	pEC50	=	8.3	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	209	4	3	2	-0.2	CC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	44367567	168635	3	None	-	1	Human	6.4	pEC50	=	6.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@H]2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL440730	168635	3	None	-	1	Human	6.4	pEC50	=	6.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@H]2CCNC2)c[nH]1	10.1021/jm030905y
	58227163	175889	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175889	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175889	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	175746	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	175746	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	175746	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	156013896	177665	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	127049855	139698	0	None	-1	4	Human	6.3	pEC50	=	6.3	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	139698	0	None	-1	4	Human	6.3	pEC50	=	6.3	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11957573	1979	29	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	1250	1979	29	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	3692	1979	29	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	CHEMBL19439	1979	29	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	155513612	175746	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	127051301	139670	0	None	-	2	Mouse	6.3	pEC50	=	6.3	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139670	0	None	-	2	Mouse	6.3	pEC50	=	6.3	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1901	114	None	-3	23	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1247	1901	114	None	-3	23	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1375	1901	114	None	-3	23	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	774	1901	114	None	-3	23	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90	1901	114	None	-3	23	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	DB05381	1901	114	None	-3	23	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	46235428	6526	0	None	-	1	Mouse	6.3	pEC50	=	6.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6526	0	None	-	1	Mouse	6.3	pEC50	=	6.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1251	1983	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	3035842	1983	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	CHEMBL18661	1983	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	1251	1983	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	3035842	1983	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	CHEMBL18661	1983	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	11206058	103936	9	None	-10	2	Human	4.2	pEC50	=	4.2	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL310360	103936	9	None	-10	2	Human	4.2	pEC50	=	4.2	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	127049855	139698	0	None	-	4	Mouse	6.2	pEC50	=	6.2	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	139698	0	None	-	4	Mouse	6.2	pEC50	=	6.2	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25149708	187852	0	None	-	0	Human	8.2	pEC50	=	8.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	319	7	4	4	2.2	CC(CC(=O)NC(=N)NCCCc1c[nH]cn1)c1cccs1	10.1021/jm800841w
	CHEMBL502339	187852	0	None	-	0	Human	8.2	pEC50	=	8.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	319	7	4	4	2.2	CC(CC(=O)NC(=N)NCCCc1c[nH]cn1)c1cccs1	10.1021/jm800841w
	58227401	175877	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175877	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175877	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	46887079	8424	0	None	-	0	Human	5.2	pEC50	=	5.2	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)CC(C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1094142	8424	0	None	-	0	Human	5.2	pEC50	=	5.2	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)CC(C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	127051301	139670	0	None	5	2	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139670	0	None	5	2	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130914	186671	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL492677	186671	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	156015638	177697	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177697	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177697	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	58227163	175889	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175889	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175889	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25149613	178621	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	382	8	4	5	2.7	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1nccs1	10.1021/jm800841w
	CHEMBL472441	178621	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	382	8	4	5	2.7	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1nccs1	10.1021/jm800841w
	136773074	156823	0	None	-	1	Human	5.2	pEC50	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4077914	156823	0	None	-	1	Human	5.2	pEC50	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	42625221	188997	0	None	-	1	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	338	7	4	2	1.9	N/C(=N\C(=O)CCc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL514641	188997	0	None	-	1	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	338	7	4	2	1.9	N/C(=N\C(=O)CCc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	24798144	109636	2	None	-19	3	Mouse	8.1	pEC50	=	8.1	Binding	Partial agonist activity at mouse histamine H4 receptorPartial agonist activity at mouse histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109636	2	None	-19	3	Mouse	8.1	pEC50	=	8.1	Binding	Partial agonist activity at mouse histamine H4 receptorPartial agonist activity at mouse histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	10374457	28682	2	None	-	2	Human	5.2	pEC50	=	5.2	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	123	1	2	2	0.2	N[C@H]1C[C@@H]1c1c[nH]cn1	10.1021/jm030905y
	CHEMBL13795	28682	2	None	-	2	Human	5.2	pEC50	=	5.2	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	123	1	2	2	0.2	N[C@H]1C[C@@H]1c1c[nH]cn1	10.1021/jm030905y
	11819221	45503	3	None	-	1	Human	6.1	pEC50	=	6.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(CC2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153061	45503	3	None	-	1	Human	6.1	pEC50	=	6.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(CC2CCNC2)c[nH]1	10.1021/jm030905y
	1204	1901	114	None	-3	23	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	1247	1901	114	None	-3	23	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	1375	1901	114	None	-3	23	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	774	1901	114	None	-3	23	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	CHEMBL90	1901	114	None	-3	23	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	DB05381	1901	114	None	-3	23	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	90667229	108982	0	None	-	0	Human	6.1	pEC50	=	6.1	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	319	7	3	3	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1cccs1	10.1039/C3MD00245D
	CHEMBL3220630	108982	0	None	-	0	Human	6.1	pEC50	=	6.1	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	319	7	3	3	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1cccs1	10.1039/C3MD00245D
	11546127	95040	2	None	-	1	Human	5.1	pEC50	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL25725	95040	2	None	-	1	Human	5.1	pEC50	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	182527	110079	7	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	167	4	4	2	-0.2	N=C(N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL325327	110079	7	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	167	4	4	2	-0.2	N=C(N)NCCCc1c[nH]cn1	10.1021/jm9000693
	46887042	8674	0	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096430	8674	0	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	25149521	178474	0	None	-	1	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1021/jm800841w
	CHEMBL471413	178474	0	None	-	1	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1021/jm800841w
	155552061	175904	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175904	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175904	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	155517880	176093	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176093	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176093	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	1249	1978	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	13499360	1978	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	CHEMBL90019	1978	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	1249	1978	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	13499360	1978	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90019	1978	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	1204	1901	114	None	-3	23	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	1247	1901	114	None	-3	23	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	1375	1901	114	None	-3	23	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	774	1901	114	None	-3	23	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	CHEMBL90	1901	114	None	-3	23	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	DB05381	1901	114	None	-3	23	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	4024	1984	14	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	9989505	1984	14	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	CHEMBL82298	1984	14	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	1204	1901	114	None	-15	23	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1901	114	None	-15	23	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1901	114	None	-15	23	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1901	114	None	-15	23	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1901	114	None	-15	23	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1901	114	None	-15	23	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	16718567	8078	0	None	-	1	Human	9.0	pIC50	=	9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091874	8078	0	None	-	1	Human	9.0	pIC50	=	9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885297	8330	0	None	-	0	Human	6.0	pIC50	=	6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	318	1	1	6	2.5	CN1CCN(c2nc(O)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093438	8330	0	None	-	0	Human	6.0	pIC50	=	6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	318	1	1	6	2.5	CN1CCN(c2nc(O)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	17747460	61000	8	None	-	4	Human	5.0	pIC50	=	5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	640	14	0	6	7.8	O=c1c2ccccc2c(Cc2ccc(Cl)cc2)nn1C[C@H]1CCCN1CCCCc1ccc(OCCCN2CCCCCC2)cc1	10.1021/jm1013874
	CHEMBL1767164	61000	8	None	-	4	Human	5.0	pIC50	=	5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	640	14	0	6	7.8	O=c1c2ccccc2c(Cc2ccc(Cl)cc2)nn1C[C@H]1CCCN1CCCCc1ccc(OCCCN2CCCCCC2)cc1	10.1021/jm1013874
	46884945	8161	0	None	-	0	Human	5.0	pIC50	=	5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	330	1	0	5	3.6	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CC(C)N1C	10.1016/j.bmcl.2010.02.097
	CHEMBL1092331	8161	0	None	-	0	Human	5.0	pIC50	=	5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	330	1	0	5	3.6	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CC(C)N1C	10.1016/j.bmcl.2010.02.097
	71547931	182529	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3c3ncnn23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4794724	182529	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3c3ncnn23)C1	10.1021/acs.jmedchem.7b01855
	11335478	160418	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL4115693	160418	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	1224	1404	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	3100	1404	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	8980	1404	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	916	1404	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	CHEMBL657	1404	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	DB01075	1404	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	11709443	8162	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092332	8162	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71548405	181577	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	0	7	1.1	CN(C)C1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782441	181577	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	0	7	1.1	CN(C)C1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	46884998	7895	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	1	1	5	2.6	NC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090532	7895	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	1	1	5	2.6	NC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	25112105	8079	0	None	-	1	Human	7.9	pIC50	=	7.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091875	8079	0	None	-	1	Human	7.9	pIC50	=	7.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	71547681	182363	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.7	Clc1cnc2nc(N3CCNCC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4792733	182363	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.7	Clc1cnc2nc(N3CCNCC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	46885128	7888	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.2	CNC1(C)CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090519	7888	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.2	CNC1(C)CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885129	7889	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	1	5	3.2	Clc1ccc2oc3c(N4CC5CCCNC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090520	7889	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	1	5	3.2	Clc1ccc2oc3c(N4CC5CCCNC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	46885241	8191	0	None	-	0	Human	4.9	pIC50	=	4.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	2	1	5	3.8	CN[C@@H]1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092543	8191	0	None	-	0	Human	4.9	pIC50	=	4.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	2	1	5	3.8	CN[C@@H]1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	156013896	177665	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	16656869	8020	0	None	-	0	Human	5.8	pIC50	=	5.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.1	CNC1CCN(c2nc(C)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091559	8020	0	None	-	0	Human	5.8	pIC50	=	5.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.1	CNC1CCN(c2nc(C)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	124087	1362	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	7157	1362	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	814	1362	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	CHEMBL1172	1362	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	DB00967	1362	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	11566111	8262	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1CC2CCC1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092933	8262	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1CC2CCC1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71547932	182185	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3c3ncnn23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4790239	182185	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3c3ncnn23)CC1	10.1021/acs.jmedchem.7b01855
	13680692	182852	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	313	2	0	8	0.9	CN1CCN(c2nc3ccc([N+](=O)[O-])cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4798729	182852	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	313	2	0	8	0.9	CN1CCN(c2nc3ccc([N+](=O)[O-])cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885239	8110	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092064	8110	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2010.02.097
	71547762	182137	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.7	CN1CCN(c2nc3nc(Cl)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789645	182137	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.7	CN1CCN(c2nc3nc(Cl)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	2063135	8293	9	None	-	1	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093180	8293	9	None	-	1	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	11695388	8160	0	None	-	0	Human	5.7	pIC50	=	5.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.2	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CCN1C	10.1016/j.bmcl.2010.02.097
	CHEMBL1092330	8160	0	None	-	0	Human	5.7	pIC50	=	5.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.2	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CCN1C	10.1016/j.bmcl.2010.02.097
	9904236	13962	16	None	-	1	Human	5.7	pIC50	=	5.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL1197564	13962	16	None	-	1	Human	5.7	pIC50	=	5.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46884997	7639	0	None	-	0	Human	4.7	pIC50	=	4.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	0	5	3.2	CN(C)C1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088888	7639	0	None	-	0	Human	4.7	pIC50	=	4.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	0	5	3.2	CN(C)C1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1530	2151	44	None	-21379	20	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	3827	2151	44	None	-21379	20	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	7206	2151	44	None	-21379	20	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	CHEMBL534	2151	44	None	-21379	20	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	DB00920	2151	44	None	-21379	20	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	20889070	181290	5	None	-	0	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	302	1	0	6	1.7	CN1CCN(c2nc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778864	181290	5	None	-	0	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	302	1	0	6	1.7	CN1CCN(c2nc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	820836	102468	10	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	268	1	0	6	1.0	CN1CCN(c2nc3ccccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL306316	102468	10	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	268	1	0	6	1.0	CN1CCN(c2nc3ccccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	121237834	146826	0	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL3929465	146826	0	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46884996	7915	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	Clc1ccc2oc3c(N4CCN5CCCC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090655	7915	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	Clc1ccc2oc3c(N4CCN5CCCC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	71548223	181278	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	401	3	0	8	2.0	CN1CCN(c2nc3nc(OCC(F)(F)F)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778712	181278	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	401	3	0	8	2.0	CN1CCN(c2nc3nc(OCC(F)(F)F)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71547685	182408	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	294	1	0	8	0.3	CN1CCN(c2nc3ncc(C#N)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4793425	182408	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	294	1	0	8	0.3	CN1CCN(c2nc3ncc(C#N)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548412	1878	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1878	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1878	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	71547764	181771	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	328	1	0	8	0.9	CN1CCN(c2nc3nc(C#N)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4784683	181771	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	328	1	0	8	0.9	CN1CCN(c2nc3nc(C#N)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548498	181658	0	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	348	1	0	8	0.6	CN1CCN(c2nc3ncc(Br)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4783487	181658	0	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	348	1	0	8	0.6	CN1CCN(c2nc3ncc(Br)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548031	182326	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.4	CN1CCN(c2nc3ncc(C(F)(F)F)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4792352	182326	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.4	CN1CCN(c2nc3ncc(C(F)(F)F)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	16656871	7894	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090531	7894	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2119	79	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	1279	2119	79	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	4908365	2119	79	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	CHEMBL129198	2119	79	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	71548412	1878	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1878	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1878	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	71548319	182656	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	CN1CCCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4796300	182656	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	CN1CCCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885178	7632	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	336	2	1	5	3.2	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088859	7632	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	336	2	1	5	3.2	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2010.02.097
	71548224	181946	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	361	1	0	7	1.5	Cc1c(Br)cnc2nc(N3CCN(C)CC3)c3nncn3c12	10.1021/acs.jmedchem.7b01855
	CHEMBL4787243	181946	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	361	1	0	7	1.5	Cc1c(Br)cnc2nc(N3CCN(C)CC3)c3nncn3c12	10.1021/acs.jmedchem.7b01855
	71547599	181486	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3ncccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781409	181486	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3ncccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71547680	182245	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3cccnc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4791152	182245	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3cccnc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	11709905	8286	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	342	2	0	5	3.7	CN1CCN(c2nc(C3CC3)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093138	8286	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	342	2	0	5	3.7	CN1CCN(c2nc(C3CC3)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	16656872	7830	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090190	7830	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	71548027	181268	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	395	1	0	7	1.0	CN1CCN(c2nc3ncc(I)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778591	181268	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	395	1	0	7	1.0	CN1CCN(c2nc3ncc(I)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	3934857	8019	23	None	-	0	Human	7.4	pIC50	=	7.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.1	Cc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091558	8019	23	None	-	0	Human	7.4	pIC50	=	7.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.1	Cc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	71547282	182491	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1C	10.1021/acs.jmedchem.7b01855
	CHEMBL4794336	182491	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1C	10.1021/acs.jmedchem.7b01855
	1278	2119	79	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2119	79	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2119	79	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2119	79	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	89477685	182992	0	None	-	0	Human	5.4	pIC50	=	5.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	1	7	1.1	CN[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4800462	182992	0	None	-	0	Human	5.4	pIC50	=	5.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	1	7	1.1	CN[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	89477651	182866	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	315	1	0	7	1.2	CN1C[C@@H]2C[C@H]1CN2c1nc2ncc(Cl)cc2n2cnnc12	10.1021/acs.jmedchem.7b01855
	CHEMBL4798915	182866	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	315	1	0	7	1.2	CN1C[C@@H]2C[C@H]1CN2c1nc2ncc(Cl)cc2n2cnnc12	10.1021/acs.jmedchem.7b01855
	11638468	8263	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1C2CCC1CN(c1ncnc3c1oc1ccc(Cl)cc13)C2	10.1016/j.bmcl.2010.02.097
	CHEMBL1092934	8263	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1C2CCC1CN(c1ncnc3c1oc1ccc(Cl)cc13)C2	10.1016/j.bmcl.2010.02.097
	71547281	182705	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	2	0	7	1.5	CN(C)[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4796842	182705	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	2	0	7	1.5	CN(C)[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	1267	3744	43	None	-33	25	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	89477709	182112	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	1	7	0.9	Clc1cnc2nc(N3C[C@@H]4C[C@H]3CN4)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789385	182112	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	1	7	0.9	Clc1cnc2nc(N3C[C@@H]4C[C@H]3CN4)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547597	182549	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	283	1	0	7	0.7	Cc1cnc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4795091	182549	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	283	1	0	7	0.7	Cc1cnc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547848	182455	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	329	1	0	7	1.6	Clc1cnc2nc(N3CCN4CCCC4C3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4793974	182455	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	329	1	0	7	1.6	Clc1cnc2nc(N3CCN4CCCC4C3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547765	181906	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	3	0	8	1.5	CCOc1nc2nc(N3CCN(C)CC3)c3nncn3c2cc1Cl	10.1021/acs.jmedchem.7b01855
	CHEMBL4786816	181906	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	3	0	8	1.5	CCOc1nc2nc(N3CCN(C)CC3)c3nncn3c2cc1Cl	10.1021/acs.jmedchem.7b01855
	71547682	181620	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	275	1	1	7	0.5	NC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782945	181620	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	275	1	1	7	0.5	NC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	89477439	181494	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	290	2	1	8	0.1	CNC1CN(c2nc3ncc(Cl)cc3n3nnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781484	181494	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	290	2	1	8	0.1	CNC1CN(c2nc3ncc(Cl)cc3n3nnnc23)C1	10.1021/acs.jmedchem.7b01855
	58475282	181070	0	None	-	0	Human	6.2	pIC50	=	6.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	282	1	0	6	1.3	Cc1ccc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4776004	181070	0	None	-	0	Human	6.2	pIC50	=	6.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	282	1	0	6	1.3	Cc1ccc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71548411	181997	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	304	1	0	8	0.5	CN1CCN(c2nc3ncc(Cl)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4787983	181997	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	304	1	0	8	0.5	CN1CCN(c2nc3ncc(Cl)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	53380276	182222	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	0	5	2.3	CN1CCN(c2cc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4790726	182222	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	0	5	2.3	CN1CCN(c2cc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885181	8253	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	293	2	1	6	2.0	CNC1CCN(c2ncnc3c2oc2ccc(C#N)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092880	8253	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	293	2	1	6	2.0	CNC1CCN(c2ncnc3c2oc2ccc(C#N)cc23)C1	10.1016/j.bmcl.2010.02.097
	89477539	182128	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.9	N[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789543	182128	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.9	N[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	71515465	181562	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782303	181562	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	71547683	181222	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	1	0	7	1.2	CN1CCN(c2nc3ncc(Br)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778068	181222	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	1	0	7	1.2	CN1CCN(c2nc3ncc(Br)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	89477445	181468	0	None	-	0	Human	6.1	pIC50	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	1	7	1.1	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781143	181468	0	None	-	0	Human	6.1	pIC50	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	1	7	1.1	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1	10.1021/acs.jmedchem.7b01855
	71547678	182758	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4797517	182758	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	24994634	2111	26	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2111	26	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2111	26	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	11682208	8190	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092542	8190	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	46885177	8045	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	282	2	1	5	2.5	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091729	8045	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	282	2	1	5	2.5	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2119	79	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2119	79	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2119	79	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2119	79	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	16656874	8022	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091569	8022	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885298	8331	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	332	2	0	6	2.8	COc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093439	8331	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	332	2	0	6	2.8	COc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	16656870	7640	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CNC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088889	7640	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CNC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2119	79	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1279	2119	79	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	4908365	2119	79	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL129198	2119	79	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	71525681	89298	0	None	2	6	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89298	0	None	2	6	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846300	79856	3	None	-2	4	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79856	3	None	-2	4	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	156013896	177665	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	944	89	None	-57	91	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	944	89	None	-57	91	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	944	89	None	-57	91	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	944	89	None	-57	91	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	944	89	None	-57	91	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	24994634	2111	26	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2111	26	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2111	26	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	11846300	79856	3	None	-2	4	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79856	3	None	-2	4	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	71519674	89299	0	None	1	5	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89299	0	None	1	5	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	1204	1901	114	None	-12	23	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-12	23	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-12	23	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-12	23	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-12	23	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-12	23	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	135398737	944	89	None	-57	91	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	944	89	None	-57	91	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	944	89	None	-57	91	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	944	89	None	-57	91	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	944	89	None	-57	91	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	155513612	175746	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156015638	177697	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177697	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177697	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-3	23	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1247	1901	114	None	-3	23	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1375	1901	114	None	-3	23	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	774	1901	114	None	-3	23	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	CHEMBL90	1901	114	None	-3	23	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	DB05381	1901	114	None	-3	23	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	119570	3110	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	2233	3110	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	953	3110	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	CHEMBL301265	3110	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	DB00413	3110	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	168268716	192160	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5170276	192160	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221176	192160	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168268680	192164	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5172675	192164	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221210	192164	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	168268827	192177	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5179906	192177	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221314	192177	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	168268853	192183	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5180504	192183	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221353	192183	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	168273471	192199	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173079	192199	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221433	192199	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168273549	192201	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174342	192201	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221438	192201	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	168273640	192203	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5176229	192203	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221444	192203	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168273745	192206	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5178472	192206	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221451	192206	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	168274129	192207	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5176780	192207	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221468	192207	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168275750	192224	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5175558	192224	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221581	192224	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	168276867	192233	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174278	192233	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221653	192233	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	168277996	192244	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173373	192244	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221736	192244	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	168278471	192246	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183205	192246	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221757	192246	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	168278543	192249	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184846	192249	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221764	192249	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168280339	192263	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5188592	192263	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221885	192263	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168281536	192279	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5189006	192279	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221966	192279	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	168281746	192280	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184911	192280	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221974	192280	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	168283097	192291	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	CHEMBL5186770	192291	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222043	192291	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	168283321	192293	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183015	192293	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222057	192293	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	168283536	192298	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5187758	192298	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222074	192298	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	168284289	192304	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5194860	192304	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222123	192304	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168284480	192308	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5197548	192308	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222137	192308	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	168286141	192322	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5192993	192322	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222231	192322	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	168286426	192324	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5198795	192324	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222241	192324	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168290235	192366	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5201074	192366	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222491	192366	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168290815	192371	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5200771	192371	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222529	192371	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168291233	192377	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5200106	192377	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222556	192377	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	168292954	192395	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5202592	192395	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222660	192395	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	168294161	192404	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204739	192404	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222742	192404	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	168294445	192408	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208113	192408	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222768	192408	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	168294166	192412	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209081	192412	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222775	192412	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	168294449	192416	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204599	192416	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222793	192416	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	168294767	192417	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207281	192417	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222802	192417	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168294770	192418	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208327	192418	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222806	192418	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	168294776	192424	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209479	192424	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222831	192424	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	168295528	192429	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5206565	192429	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222872	192429	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168294178	192433	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5205197	192433	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222887	192433	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168295541	192438	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208845	192438	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222926	192438	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168295543	192439	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209021	192439	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222929	192439	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	168295803	192443	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207206	192443	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222937	192443	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168294182	192446	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208722	192446	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222960	192446	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	156013896	177665	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156015638	177697	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177697	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177697	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	175746	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	177665	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	944	89	None	-57	91	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	944	89	None	-57	91	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	944	89	None	-57	91	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	944	89	None	-57	91	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	944	89	None	-57	91	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	71525679	89296	0	None	6	6	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89296	0	None	6	6	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	155513612	175746	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	11846300	79856	3	None	-2	4	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79856	3	None	-2	4	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	24745335	292	7	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	1278	2119	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	1279	2119	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	4908365	2119	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	1223	940	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	2790	940	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	CHEMBL14690	940	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	1223	940	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	2790	940	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	CHEMBL14690	940	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	156013896	177665	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	175746	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	177665	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	56835171	69397	0	None	1	9	Human	7.4	pKd	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL1935574	69397	0	None	1	9	Human	7.4	pKd	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	155513612	175746	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	177665	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-15	23	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-15	23	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-15	23	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-15	23	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-15	23	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-15	23	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	156013896	177665	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-3	23	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1901	114	None	-3	23	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1901	114	None	-3	23	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1901	114	None	-3	23	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1901	114	None	-3	23	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1901	114	None	-3	23	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	156013896	177665	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	175746	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175746	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156013896	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156013896	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	944	89	None	-57	91	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	944	89	None	-57	91	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	944	89	None	-57	91	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	944	89	None	-57	91	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	944	89	None	-57	91	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	11846300	79856	3	None	-2	4	Human	7.1	pKd	=	7.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79856	3	None	-2	4	Human	7.1	pKd	=	7.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	156015638	177697	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177697	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177697	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156015638	177697	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177697	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177697	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	71525678	89295	0	None	5	6	Human	8.1	pKd	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89295	0	None	5	6	Human	8.1	pKd	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	156013896	177665	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	54587788	61206	0	None	-	1	Human	10.4	pKi	=	10.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	4	3	5	1.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770975	61206	0	None	-	1	Human	10.4	pKi	=	10.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	4	3	5	1.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	23653543	67706	2	None	-	1	Human	10.0	pKi	=	10	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914541	67706	2	None	-	1	Human	10.0	pKi	=	10	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	54587787	61202	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770971	61202	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	46235428	6526	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6526	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	54586763	61199	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	3	2	5	1.9	CN1CCN(c2cc(N)nc(NCC(C)(C)C)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770968	61199	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	3	2	5	1.9	CN1CCN(c2cc(N)nc(NCC(C)(C)C)c2)CC1	10.1016/j.bmcl.2011.03.017
	24826169	67698	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914462	67698	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	16718567	8078	0	None	-	1	Human	9.4	pKi	=	9.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1091874	8078	0	None	-	1	Human	9.4	pKi	=	9.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737950	67789	0	None	-	1	Human	9.2	pKi	=	9.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	361	2	2	6	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914781	67789	0	None	-	1	Human	9.2	pKi	=	9.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	361	2	2	6	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	58227148	116631	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	116631	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	57399746	67764	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914755	67764	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	54580885	61204	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	4	3	5	1.8	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770973	61204	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	4	3	5	1.8	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	24826766	67759	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914750	67759	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	59777338	109639	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236556	109639	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	1204	1901	114	None	-3	23	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1247	1901	114	None	-3	23	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1375	1901	114	None	-3	23	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	774	1901	114	None	-3	23	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	CHEMBL90	1901	114	None	-3	23	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	DB05381	1901	114	None	-3	23	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1223	940	32	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	940	32	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	940	32	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	54581940	61223	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770992	61223	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	24797598	61233	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771001	61233	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	11224088	135934	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1021/jm800876b
	CHEMBL373579	135934	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1021/jm800876b
	24798144	109636	2	None	19	3	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3236553	109636	2	None	19	3	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	58227342	116618	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393534	116618	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	11224088	135934	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	CHEMBL373579	135934	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	24825998	67769	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914760	67769	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)CC1	10.1016/j.bmcl.2011.08.014
	57396301	67791	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	2.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914783	67791	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	2.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2011.08.014
	127050310	140304	0	None	-	1	Human	9.0	pKi	=	9	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	347	3	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(C(C)(F)F)cc23)C1	10.1039/C6MD00108D
	CHEMBL3819260	140304	0	None	-	1	Human	9.0	pKi	=	9	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	347	3	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(C(C)(F)F)cc23)C1	10.1039/C6MD00108D
	46222048	8896	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1098230	8896	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1016/j.bmc.2010.04.052
	24994542	109637	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236554	109637	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	24948752	67705	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.4	Nc1nc(N2CCCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914540	67705	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.4	Nc1nc(N2CCCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	24825727	67707	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914542	67707	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	46222048	8896	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Inhibition of human recombinant histamine H4 receptor expressed in CHO cellsInhibition of human recombinant histamine H4 receptor expressed in CHO cells	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1021/jm901890s
	CHEMBL1098230	8896	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Inhibition of human recombinant histamine H4 receptor expressed in CHO cellsInhibition of human recombinant histamine H4 receptor expressed in CHO cells	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1021/jm901890s
	155524115	175973	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	175973	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	175973	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	24798144	109636	2	None	19	3	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109636	2	None	19	3	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	155524115	175973	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	175973	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	175973	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	24797604	109635	2	None	-	1	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	CHEMBL3236552	109635	2	None	-	1	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	44231618	5813	0	None	1	4	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1079516	5813	0	None	1	4	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	25128824	192575	0	None	3	2	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192575	0	None	3	2	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	24992077	109654	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236571	109654	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	44231618	5813	0	None	-1	4	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1079516	5813	0	None	-1	4	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	44129130	197875	0	None	2	2	Rat	8.8	pKi	=	8.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	197875	0	None	2	2	Rat	8.8	pKi	=	8.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1223	940	32	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	940	32	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	940	32	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	44187557	193827	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202332	193827	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL553423	193827	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1901	114	None	-3	23	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	1247	1901	114	None	-3	23	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	1375	1901	114	None	-3	23	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	774	1901	114	None	-3	23	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	CHEMBL90	1901	114	None	-3	23	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	DB05381	1901	114	None	-3	23	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	11957573	1979	29	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	1250	1979	29	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	3692	1979	29	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	CHEMBL19439	1979	29	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	16202258	67876	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915536	67876	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	66740859	109662	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	327	3	2	5	2.3	CN[C@@H]1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236579	109662	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	327	3	2	5	2.3	CN[C@@H]1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)C1	10.1021/jm401727m
	24798232	109643	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	1.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236560	109643	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	1.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCCN[C@H]3C2)n1	10.1021/jm401727m
	44231735	7703	0	None	1	4	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089390	7703	0	None	1	4	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	25129527	186831	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	186831	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	135525668	67870	0	None	263	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915348	67870	0	None	263	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	25217643	111678	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysis	ChEMBL	289	2	0	5	1.9	CN1CCN(c2ncnc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290570	111678	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysis	ChEMBL	289	2	0	5	1.9	CN1CCN(c2ncnc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1249	1978	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	13499360	1978	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90019	1978	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10176414	4000	13	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1246	4000	13	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL281576	4000	13	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10060212	202829	0	None	5	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72769	202829	0	None	5	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681807	89216	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	8	3	8	1.6	CC(C)CCc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375668	89216	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	8	3	8	1.6	CC(C)CCc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681804	89241	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1ccnc(N)n1)c1cc(-c2ccccc2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375770	89241	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1ccnc(N)n1)c1cc(-c2ccccc2)nc(N)n1	10.1021/jm301886t
	1223	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1223	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1249	1978	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	13499360	1978	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	CHEMBL90019	1978	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	53233802	61218	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770987	61218	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	70692529	75331	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	303	2	1	2	2.7	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047466	75331	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	303	2	1	2	2.7	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	46228994	197748	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	466	6	1	7	2.7	CC1CCCCN1S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591249	197748	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	466	6	1	7	2.7	CC1CCCCN1S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	1223	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	2790	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	CHEMBL14690	940	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	45268092	194340	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1743881	194340	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560111	194340	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45268093	194341	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202321	194341	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560112	194341	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45267230	194808	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202318	194808	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL563342	194808	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	24737954	67787	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(F)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914778	67787	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(F)c23)C1	10.1016/j.bmcl.2011.08.014
	24826522	67794	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914786	67794	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	24826168	67797	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914789	67797	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24745335	292	7	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	71525680	89301	0	None	97	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	284	3	2	6	1.3	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376806	89301	0	None	97	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	284	3	2	6	1.3	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	71525678	89295	0	None	5	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89295	0	None	5	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25101945	192504	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192504	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	136192886	56186	0	None	-1	4	Mouse	7.0	pKi	=	7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56186	0	None	-1	4	Mouse	7.0	pKi	=	7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	44129134	197827	0	None	-22	2	Rat	7.0	pKi	=	7	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	197827	0	None	-22	2	Rat	7.0	pKi	=	7	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11694543	75447	4	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	6	2	2	1.3	CCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204843	75447	4	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	6	2	2	1.3	CCNCCCCc1c[nH]cn1	10.1021/jm0504353
	11521414	140766	0	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	2	2	2.7	c1nc(CCCCNC2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL383324	140766	0	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	2	2	2.7	c1nc(CCCCNC2CCCCC2)c[nH]1	10.1021/jm0504353
	9812843	203533	1	None	-4	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1016/j.bmcl.2011.06.123
	CHEMBL78838	203533	1	None	-4	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1016/j.bmcl.2011.06.123
	53233904	61196	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770965	61196	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	39732699	75328	2	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	1	2	2	1.4	O=C(c1cc2cccc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047463	75328	2	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	1	2	2	1.4	O=C(c1cc2cccc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	70696623	75336	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	2	2	2	2.0	CNC1CN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047471	75336	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	2	2	2	2.0	CNC1CN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	51354298	58579	2	None	1	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	233	4	1	4	1.9	CC(C)(C)c1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688944	58579	2	None	1	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	233	4	1	4	1.9	CC(C)(C)c1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	45267237	194528	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202320	194528	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL561451	194528	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	53378933	109656	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	269	3	2	5	1.2	Nc1nc(Cc2ccccc2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236573	109656	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	269	3	2	5	1.2	Nc1nc(Cc2ccccc2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	11087453	75292	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL2047270	75292	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1039/C2MD20212C
	44395335	66727	0	None	25	2	Human	6.0	pKi	=	6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	266	4	2	3	1.5	CN(C)CCNC(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL187210	66727	0	None	25	2	Human	6.0	pKi	=	6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	266	4	2	3	1.5	CN(C)CCNC(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	10978141	5081	1	None	-4	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL105803	5081	1	None	-4	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	71681477	89228	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	398	7	4	8	3.3	Nc1nc(NCCNc2cc(-c3ccccc3)nc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375758	89228	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	398	7	4	8	3.3	Nc1nc(NCCNc2cc(-c3ccccc3)nc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	71681802	89239	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1cc(NCCNc2ccnc(N)n2)ncn1	10.1021/jm301886t
	CHEMBL2375769	89239	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1cc(NCCNc2ccnc(N)n2)ncn1	10.1021/jm301886t
	206876	18756	7	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL128637	18756	7	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	66572107	75287	1	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047265	75287	1	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	66572115	75307	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047442	75307	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	11469514	103691	2	None	-64	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL309654	103691	2	None	-64	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	46890591	6947	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	436	6	1	4	4.8	Cc1cc(OCCCN2CC3CCCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084811	6947	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	436	6	1	4	4.8	Cc1cc(OCCCN2CC3CCCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44275807	98513	0	None	-9120	10	Human	5.0	pKi	=	5	Binding	Binding affinity towards human histaminergic (H4) receptorBinding affinity towards human histaminergic (H4) receptor	ChEMBL	422	7	0	4	4.5	C[C@H]1CC[C@H](C)N1CCCOc1ccc(-c2ccc(C(=O)N3CCOCC3)cc2)cc1	10.1016/s0960-894x(03)00118-5
	CHEMBL27979	98513	0	None	-9120	10	Human	5.0	pKi	=	5	Binding	Binding affinity towards human histaminergic (H4) receptorBinding affinity towards human histaminergic (H4) receptor	ChEMBL	422	7	0	4	4.5	C[C@H]1CC[C@H](C)N1CCCOc1ccc(-c2ccc(C(=O)N3CCOCC3)cc2)cc1	10.1016/s0960-894x(03)00118-5
	4242418	116929	11	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	2	1	2	1.9	CN1CCN(Cc2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL339826	116929	11	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	2	1	2	1.9	CN1CCN(Cc2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	71681978	89223	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	310	5	3	5	1.7	CN(CCNc1ccnc(N)n1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm301886t
	CHEMBL2375753	89223	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	310	5	3	5	1.7	CN(CCNc1ccnc(N)n1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm301886t
	58227158	116633	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1cc(N2CCN(C)CC2)cnn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393549	116633	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1cc(N2CCN(C)CC2)cnn1	10.1016/j.bmcl.2014.12.027
	57391926	69559	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938967	69559	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11846300	79856	3	None	-2	4	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL213912	79856	3	None	-2	4	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2015.12.035
	24854583	95676	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	304	2	0	4	3.0	CN1CCN(c2nc3ccccc3nc2-c2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260372	95676	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	304	2	0	4	3.0	CN1CCN(c2nc3ccccc3nc2-c2ccccc2)CC1	10.1021/jm7014217
	16082860	138082	0	None	-7	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL377572	138082	0	None	-7	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	57391032	67758	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@H]3C[C@@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914749	67758	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@H]3C[C@@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25179297	173259	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL453877	173259	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	24759518	178729	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL473302	178729	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178342	186204	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489070	186204	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179297	173259	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL453877	173259	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25178342	186204	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489070	186204	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	71518535	86060	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	1	5	2.6	CN1CCN(c2nc(N(C)C)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314763	86060	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	1	5	2.6	CN1CCN(c2nc(N(C)C)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	10978141	5081	1	None	-4	2	Human	6.0	pKi	=	6.0	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL105803	5081	1	None	-4	2	Human	6.0	pKi	=	6.0	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	57393730	67542	0	None	-2754	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	579	8	2	6	5.0	O=C(c1cc2ccccc2[nH]1)N1CCN(CCN2CCC(Nc3nc4ccccc4n3Cc3ccc(F)cc3)CC2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910386	67542	0	None	-2754	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	579	8	2	6	5.0	O=C(c1cc2ccccc2[nH]1)N1CCN(CCN2CCC(Nc3nc4ccccc4n3Cc3ccc(F)cc3)CC2)CC1	10.1016/j.bmcl.2011.09.001
	24826681	67777	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914768	67777	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	127049855	139698	0	None	-1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	139698	0	None	-1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	57391869	62790	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774589	62790	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789349	62790	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	90645163	111698	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1cc(C)cc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290590	111698	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1cc(C)cc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	10656971	16244	4	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL123137	16244	4	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1016/j.bmcl.2011.07.114
	121238070	165934	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	4	1	7	1.4	CN1CCN(c2nc(N)nc(COc3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4278404	165934	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	4	1	7	1.4	CN1CCN(c2nc(N)nc(COc3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178555	173667	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454891	173667	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178555	173667	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454891	173667	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	155540201	172339	0	None	-67	5	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	513	16	2	4	5.5	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc(Cl)cc1	10.1039/C8MD00527C
	CHEMBL4516173	172339	0	None	-67	5	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	513	16	2	4	5.5	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc(Cl)cc1	10.1039/C8MD00527C
	1278	2119	79	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	832283	12292	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL118572	12292	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	832283	12292	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL118572	12292	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	10269628	138187	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	355	1	0	4	4.6	CC(C)N1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL377985	138187	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	355	1	0	4	4.6	CC(C)N1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	54582866	61219	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(N)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770988	61219	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(N)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2011.03.017
	25133970	187005	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL494679	187005	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	49841962	69385	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3c(-c4ccoc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935562	69385	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3c(-c4ccoc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	44547568	116614	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393530	116614	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	13731320	158104	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	2	2	2	4.0	O=C(Nc1cccc2ccccc12)N1CCC(c2c[nH]cn2)CC1	10.1016/j.ejmech.2016.09.074
	CHEMBL4092578	158104	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	2	2	2	4.0	O=C(Nc1cccc2ccccc12)N1CCC(c2c[nH]cn2)CC1	10.1016/j.ejmech.2016.09.074
	127035837	136892	0	None	-5	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	403	4	1	5	3.7	CN1CCN(c2nc(NCc3ccc(F)cc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753334	136892	0	None	-5	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	403	4	1	5	3.7	CN1CCN(c2nc(NCc3ccc(F)cc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	21842421	111679	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	270	2	1	6	0.9	CN1CCN(c2nc(N)nc(-c3ccccc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290571	111679	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	270	2	1	6	0.9	CN1CCN(c2nc(N)nc(-c3ccccc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10688021	155570	0	None	-1	2	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	332	8	1	3	4.3	O=C(/C=C/c1ccccc1)c1ccc(OCCCc2c[nH]cn2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4063170	155570	0	None	-1	2	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	332	8	1	3	4.3	O=C(/C=C/c1ccccc1)c1ccc(OCCCc2c[nH]cn2)cc1	10.1016/j.ejmech.2016.09.074
	9944571	116516	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL338937	116516	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9944571	116516	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338937	116516	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	23650961	3785	18	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	9276	3785	18	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	CHEMBL3301609	3785	18	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	DB12522	3785	18	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	24803710	149796	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	400	6	2	5	4.1	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(F)c(F)ccc2[nH]1	nan
	CHEMBL3953011	149796	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	400	6	2	5	4.1	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(F)c(F)ccc2[nH]1	nan
	25101945	192504	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192504	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	118720437	115413	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	275	3	2	5	1.0	CN[C@@H]1CCN(c2cc(CC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354811	115413	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	275	3	2	5	1.0	CN[C@@H]1CCN(c2cc(CC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	24737952	67792	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(OC)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914784	67792	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(OC)cc23)C1	10.1016/j.bmcl.2011.08.014
	9944571	116516	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338937	116516	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	24996854	109647	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	CHEMBL3236564	109647	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	71603142	89291	0	None	54	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	3	2	5	1.2	CCc1ccc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376796	89291	0	None	54	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	3	2	5	1.2	CCc1ccc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	44129282	197520	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)cc(Cl)c3n2)CC1	10.1021/jm901379s
	CHEMBL589746	197520	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)cc(Cl)c3n2)CC1	10.1021/jm901379s
	71603139	89288	0	None	13	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	2	2	5	1.5	Nc1nc(N2CCNCC2)c2ccc(C3CC3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376793	89288	0	None	13	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	2	2	5	1.5	Nc1nc(N2CCNCC2)c2ccc(C3CC3)cc2n1	10.1016/j.bmcl.2013.02.091
	45257132	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	155513612	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	44450493	96087	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	1	1	4	1.3	CN1CCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL262859	96087	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	1	1	4	1.3	CN1CCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	1278	2119	79	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	126963283	160416	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL4115677	160416	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	1224	1404	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	3100	1404	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	8980	1404	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	916	1404	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	CHEMBL657	1404	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	DB01075	1404	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	1241	2006	46	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	1242	2006	46	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	3614	2006	46	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	CHEMBL507	2006	46	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	49841609	69379	13	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3c(Br)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935556	69379	13	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3c(Br)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	51354871	58597	0	None	-22	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	290	6	0	4	3.3	c1c(CC2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1688982	58597	0	None	-22	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	290	6	0	4	3.3	c1c(CC2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	164618508	185350	0	None	-363	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	6	4	4	2.7	Cc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4869194	185350	0	None	-363	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	6	4	4	2.7	Cc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	164620678	185437	0	None	-177	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.7	CCC(NC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4870510	185437	0	None	-177	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.7	CCC(NC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	58227159	175811	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4534254	175811	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595907	175811	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	122193099	123400	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	374	3	1	6	2.1	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Br)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628046	123400	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	374	3	1	6	2.1	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Br)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	51354008	58574	0	None	-3	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	253	5	1	4	1.8	c1ccc(Cc2cn(CCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688939	58574	0	None	-3	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	253	5	1	4	1.8	c1ccc(Cc2cn(CCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	51354586	58594	0	None	-15	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	2.9	CC(c1cn(CCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	CHEMBL1688978	58594	0	None	-15	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	2.9	CC(c1cn(CCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	16082824	77594	0	None	1	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL209598	77594	0	None	1	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	54585778	61220	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(N)nc(NCCO)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770989	61220	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(N)nc(NCCO)n2)CC1	10.1016/j.bmcl.2011.03.017
	58227165	116620	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393536	116620	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	24853664	95894	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261517	95894	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	10773349	8007	1	None	-11	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL109130	8007	1	None	-11	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10715857	74666	1	None	-3	3	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	398	8	1	3	3.5	O=C(CCCc1ccc(I)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL20338	74666	1	None	-3	3	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	398	8	1	3	3.5	O=C(CCCc1ccc(I)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	16082820	79708	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213250	79708	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	10776393	5422	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1076267	5422	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL156912	5422	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	118725542	116621	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393537	116621	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	1278	2119	79	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2119	79	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2119	79	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2119	79	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	90645161	111696	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3cc(F)cc(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290588	111696	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3cc(F)cc(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1278	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	45257132	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	24994634	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	24994634	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	8984	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	CHEMBL3236549	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	24994635	109653	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236570	109653	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	24826442	67765	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1ccc2oc3c(N4CCN(C)CC4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914756	67765	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1ccc2oc3c(N4CCN(C)CC4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	1278	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1278	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1204	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1247	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1375	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	774	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	DB05381	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1278	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1279	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	4908365	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	CHEMBL129198	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1204	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1204	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	1247	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1247	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	1375	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1375	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	774	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	774	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	DB05381	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	DB05381	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	155513612	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	1226	1988	16	None	2	6	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	41376	1988	16	None	2	6	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	CHEMBL12608	1988	16	None	2	6	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	24745335	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	71603135	89284	0	None	26	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	2	2	5	2.3	Nc1nc(N2CCNCC2)c2ccc(C3CCCC3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376789	89284	0	None	26	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	2	2	5	2.3	Nc1nc(N2CCNCC2)c2ccc(C3CCCC3)cc2n1	10.1016/j.bmcl.2013.02.091
	71525589	89302	0	None	11	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	286	3	2	6	1.5	CN[C@@H]1CCN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376807	89302	0	None	11	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	286	3	2	6	1.5	CN[C@@H]1CCN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	24745335	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	24994634	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	24994634	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	3035746	187597	6	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL1237146	187597	6	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL498770	187597	6	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1204	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	155513612	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	175746	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1249	1978	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	13499360	1978	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90019	1978	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1253	2027	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	132937	2027	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19010	2027	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10023327	168450	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168450	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	10023327	168450	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168450	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	10023327	168450	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168450	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	1253	2027	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	132937	2027	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL19010	2027	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	1204	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	1247	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	1375	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	774	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	DB05381	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	11065080	19236	2	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL129488	19236	2	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	66573460	75316	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.2	Cc1cc(Cl)cc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047451	75316	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.2	Cc1cc(Cl)cc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	21638785	188073	4	None	-4	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL503605	188073	4	None	-4	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	44129132	197853	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	6	1	7	1.3	CN1CCN(c2nc(NCCS(=O)(=O)N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592188	197853	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	6	1	7	1.3	CN1CCN(c2nc(NCCS(=O)(=O)N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10176414	4000	13	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	1246	4000	13	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL281576	4000	13	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	45267233	193238	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202319	193238	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL541171	193238	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	1278	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1249	1978	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	13499360	1978	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90019	1978	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	25128823	192115	0	None	-2	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192115	0	None	-2	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	127050431	139785	0	None	4	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	139785	0	None	4	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11451949	67703	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	384	6	1	4	3.9	CN1CCN(CCCOc2ccc(-c3nc4cc(Cl)ccc4[nH]3)cc2)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914533	67703	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	384	6	1	4	3.9	CN1CCN(CCCOc2ccc(-c3nc4cc(Cl)ccc4[nH]3)cc2)CC1	10.1016/j.bmcl.2011.08.014
	46228126	200925	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.4	CN1CCN(c2cc(NCc3ccc(C(F)(F)F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL612131	200925	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.4	CN1CCN(c2cc(NCc3ccc(C(F)(F)F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	60150166	174152	0	None	-323	5	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	524	17	2	6	4.7	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc([N+](=O)[O-])cc1	10.1039/C8MD00527C
	CHEMBL4560123	174152	0	None	-323	5	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	524	17	2	6	4.7	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc([N+](=O)[O-])cc1	10.1039/C8MD00527C
	168295543	192439	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209021	192439	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222929	192439	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	164610648	184255	0	None	-120	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4852212	184255	0	None	-120	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	10820930	5722	0	None	1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL107877	5722	0	None	1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	24826443	67779	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914770	67779	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	10800875	6203	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1081718	6203	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL345828	6203	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	24745335	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	1223	940	32	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	10800875	6203	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1081718	6203	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL345828	6203	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	11206058	103936	9	None	-10	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL310360	103936	9	None	-10	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	1267	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3035905	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	CHEMBL260374	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3062060	10283	5	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	221	4	6	3	-1.0	N=C(N)NCc1cccc(CNC(=N)N)n1	10.1021/jm7014777
	CHEMBL1162346	10283	5	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	221	4	6	3	-1.0	N=C(N)NCc1cccc(CNC(=N)N)n1	10.1021/jm7014777
	11511026	15860	0	None	-208	4	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3ccc4ccccc4n3)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	CHEMBL1223683	15860	0	None	-208	4	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3ccc4ccccc4n3)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	1267	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	3035905	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	CHEMBL260374	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	10106063	108783	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting method	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	CHEMBL321860	108783	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting method	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	24826000	67801	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	2	2	6	3.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914793	67801	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	2	2	6	3.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)C1	10.1016/j.bmcl.2011.08.014
	1267	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	3035905	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	CHEMBL260374	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	1278	2119	79	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10106063	108783	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321860	108783	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	9974306	202769	0	None	-15	4	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72372	202769	0	None	-15	4	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	56849569	71268	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923033	71268	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963332	71268	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11499453	75858	0	None	-79	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	2	2	2.4	CC(C)CNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205834	75858	0	None	-79	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	2	2	2.4	CC(C)CNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11672850	76320	2	None	-10	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.1	CC(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206451	76320	2	None	-10	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.1	CC(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	1267	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	3035905	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	CHEMBL260374	3744	43	None	-33	25	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	10106063	108783	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321860	108783	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	5310982	93179	4	None	-1348	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	CHEMBL24665	93179	4	None	-1348	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	71681975	89220	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	296	5	4	5	1.4	Nc1nccc(NCCNC(=O)c2cc3ccccc3[nH]2)n1	10.1021/jm301886t
	CHEMBL2375750	89220	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	296	5	4	5	1.4	Nc1nccc(NCCNC(=O)c2cc3ccccc3[nH]2)n1	10.1021/jm301886t
	71681642	89235	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nc(NCCNc2ccnc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375765	89235	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nc(NCCNc2ccnc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	11286909	203573	2	None	-1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL79126	203573	2	None	-1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	10678661	5290	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106931	5290	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	11043445	56185	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632410	56185	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	168294449	192416	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204599	192416	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222793	192416	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	66572111	75293	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047271	75293	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	118720435	115412	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	225	3	2	5	0.3	CN[C@@H]1CCN(c2cc(CF)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354809	115412	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	225	3	2	5	0.3	CN[C@@H]1CCN(c2cc(CF)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	90645151	111680	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290572	111680	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2014.06.032
	16082833	79937	0	None	-23	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214312	79937	0	None	-23	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	54584826	61224	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770993	61224	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	57395538	67869	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915347	67869	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	57395538	67869	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915347	67869	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	25178343	172022	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL448962	172022	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178343	172022	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL448962	172022	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	57403269	67775	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	1	6	2.5	CN1CCN(c2nc(N)nc3c4cc(Cl)ccc4[s+]([O-])c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914766	67775	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	1	6	2.5	CN1CCN(c2nc(N)nc3c4cc(Cl)ccc4[s+]([O-])c23)CC1	10.1016/j.bmcl.2011.08.014
	24854705	95665	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	3	0	6	2.6	CN1CCN(c2nc3ccccc3nc2Oc2cccnc2)CC1	10.1021/jm7014217
	CHEMBL260274	95665	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	3	0	6	2.6	CN1CCN(c2nc3ccccc3nc2Oc2cccnc2)CC1	10.1021/jm7014217
	164619208	185080	0	None	-446	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccccc1F	10.1016/j.ejmech.2021.113190
	CHEMBL4864852	185080	0	None	-446	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccccc1F	10.1016/j.ejmech.2021.113190
	10678661	5290	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106931	5290	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	57402929	69524	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938844	69524	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1278	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	1279	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	4908365	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	CHEMBL129198	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	57392827	67781	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914772	67781	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	58227163	175889	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175889	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175889	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	1204	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	1247	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	1375	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	774	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	DB05381	1901	114	None	-3	23	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	16082856	80701	0	None	-2	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL215628	80701	0	None	-2	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	46929647	16579	0	None	-91	3	Human	5.9	pKi	=	5.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	CHEMBL1243394	16579	0	None	-91	3	Human	5.9	pKi	=	5.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	11858011	158457	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	9	0	3	4.6	O=C(c1ccccc1)c1ccc(OCCCCCN2CCCC2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4096444	158457	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	9	0	3	4.6	O=C(c1ccccc1)c1ccc(OCCCCCN2CCCC2)cc1	10.1016/j.ejmech.2016.09.074
	16352	116683	11	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL339414	116683	11	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	11240759	104734	5	None	-190	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL311473	104734	5	None	-190	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	24853662	95629	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	3	0	5	3.1	CN1CCN(c2nc3ccccc3nc2OC2CCCCC2)CC1	10.1021/jm7014217
	CHEMBL260043	95629	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	3	0	5	3.1	CN1CCN(c2nc3ccccc3nc2OC2CCCCC2)CC1	10.1021/jm7014217
	54584378	62829	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774595	62829	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789782	62829	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164626153	185796	0	None	-6456	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccc(F)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4875600	185796	0	None	-6456	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccc(F)c1	10.1016/j.ejmech.2021.113190
	54583874	61217	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N)nc(N3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770986	61217	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N)nc(N3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	54584378	62829	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774595	62829	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789782	62829	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	46890122	7292	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	421	9	2	5	4.3	Cc1cc(OCCCNCCc2cn(C)cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086344	7292	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	421	9	2	5	4.3	Cc1cc(OCCCNCCc2cn(C)cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	155568016	175464	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	4	1	6	1.1	CCC(CC)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4590367	175464	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	4	1	6	1.1	CCC(CC)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	58227319	116624	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393540	116624	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	56849571	71269	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923034	71269	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963347	71269	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	25179126	172841	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm800876b
	CHEMBL452849	172841	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm800876b
	25179126	172841	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm901379s
	CHEMBL452849	172841	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm901379s
	164609729	184515	0	None	-2187	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856059	184515	0	None	-2187	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	164622886	185360	0	None	-131	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4869389	185360	0	None	-131	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	164626509	185753	0	None	-3	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	358	3	4	4	3.2	N/C(=N\C(=O)NC1CCCCC1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4874969	185753	0	None	-3	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	358	3	4	4	3.2	N/C(=N\C(=O)NC1CCCCC1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	11043445	56185	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL1632410	56185	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	1204	1901	114	None	-12	23	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1901	114	None	-12	23	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1901	114	None	-12	23	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1901	114	None	-12	23	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1901	114	None	-12	23	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1901	114	None	-12	23	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	11609325	14361	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.3	CN1CCN(C(=O)c2nc3c(Cl)cc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL120221	14361	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.3	CN1CCN(C(=O)c2nc3c(Cl)cc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	24825796	67753	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	NC[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914744	67753	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	NC[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	46228100	198333	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	315	4	1	6	2.1	CN1CCN(c2cc(SCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595413	198333	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	315	4	1	6	2.1	CN1CCN(c2cc(SCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	44628631	67782	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3c(Cl)cc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914773	67782	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3c(Cl)cc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	71525679	89296	0	None	-9	6	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89296	0	None	-9	6	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	4060730	19183	51	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL129199	19183	51	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	24853594	95691	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	4	0	5	3.3	Cc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL260470	95691	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	4	0	5	3.3	Cc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	44414495	79609	0	None	-34	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	5	0	2	6.3	CC1CC(C)CN(Cc2ccc(-c3ccc(CN4CC(C)CC(C)C4)cc3)cc2)C1	10.1016/j.bmc.2008.10.029
	CHEMBL212869	79609	0	None	-34	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	5	0	2	6.3	CC1CC(C)CN(Cc2ccc(-c3ccc(CN4CC(C)CC(C)C4)cc3)cc2)C1	10.1016/j.bmc.2008.10.029
	127026017	136833	0	None	-12	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752972	136833	0	None	-12	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)cc1	10.1016/j.bmcl.2015.12.035
	164614907	184001	0	None	-436	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(F)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848697	184001	0	None	-436	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(F)cc1	10.1016/j.ejmech.2021.113190
	57390263	67845	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	330	1	2	7	1.8	Nc1nc(N2CC3CCNC3C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915052	67845	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	330	1	2	7	1.8	Nc1nc(N2CC3CCNC3C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	9900313	19226	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129444	19226	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9900313	19226	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129444	19226	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0341047
	1278	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2119	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	46890592	6315	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6315	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	9900313	19226	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129444	19226	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	156013896	177665	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177665	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177665	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25129523	186704	40	None	-1	2	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	186704	40	None	-1	2	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	1249	1978	14	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	13499360	1978	14	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	CHEMBL90019	1978	14	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	1204	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1247	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1375	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	774	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	DB05381	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	137655927	158342	0	None	17	2	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	403	7	2	3	5.2	O=C(NC(c1ccc(Cl)cc1)c1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL4095224	158342	0	None	17	2	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	403	7	2	3	5.2	O=C(NC(c1ccc(Cl)cc1)c1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	71603141	89290	0	None	14	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	5	1.2	CCc1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376795	89290	0	None	14	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	5	1.2	CCc1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	54585779	61222	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770991	61222	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25179303	188757	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCCC3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL512568	188757	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCCC3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	51354304	58588	0	None	-3	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCn2cc(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688972	58588	0	None	-3	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCn2cc(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	71519016	86042	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314501	86042	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	71518859	86068	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	294	2	3	5	1.6	Nc1nc(N2CCNCC2)c2cc(-c3ccccc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314772	86068	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	294	2	3	5	1.6	Nc1nc(N2CCNCC2)c2cc(-c3ccccc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	156014317	177702	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634728	177702	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650854	177702	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	11724853	96572	0	None	-19	4	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	340	0	0	4	3.7	CN1CCN(C2=Nc3cc(Cl)ccc3N(C)c3ccccc32)CC1	10.1021/jm051008s
	CHEMBL266723	96572	0	None	-19	4	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	340	0	0	4	3.7	CN1CCN(C2=Nc3cc(Cl)ccc3N(C)c3ccccc32)CC1	10.1021/jm051008s
	57398985	67541	0	None	-1122	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	569	12	2	7	4.4	COc1ccc(CN(CCN(C)CC(C)Nc2ccc3[nH]c(C(=O)N4CCN(C)CC4)cc3c2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910385	67541	0	None	-1122	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	569	12	2	7	4.4	COc1ccc(CN(CCN(C)CC(C)Nc2ccc3[nH]c(C(=O)N4CCN(C)CC4)cc3c2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	118725539	116607	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	2	0	4	1.8	CN1CCN(c2ccnc(N3CCCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393523	116607	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	2	0	4	1.8	CN1CCN(c2ccnc(N3CCCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	16082822	79570	0	None	-4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@@H]2C[C@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL212705	79570	0	None	-4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@@H]2C[C@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	46228160	198177	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	308	3	0	6	1.6	CN1CCN(c2ncnc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594468	198177	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	308	3	0	6	1.6	CN1CCN(c2ncnc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	2306318	86580	12	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1039/C5MD00022J
	CHEMBL232421	86580	12	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1039/C5MD00022J
	127036980	136822	0	None	-16	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752885	136822	0	None	-16	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	57391033	67778	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	287	1	2	6	1.5	Nc1nc(N2CCNCC2)c2oc3cccc(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914769	67778	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	287	1	2	6	1.5	Nc1nc(N2CCNCC2)c2oc3cccc(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	1236	2036	23	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	1237	2036	23	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	156615	2036	23	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	CHEMBL268229	2036	23	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	24797248	198184	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594490	198184	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	9951970	5426	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1076300	5426	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL154797	5426	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	121238066	166139	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	2	8	-1.0	CN1CCN(c2nc(N)nc(CN3C(=O)NC(C)(C)C3=O)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4282283	166139	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	2	8	-1.0	CN1CCN(c2nc(N)nc(CN3C(=O)NC(C)(C)C3=O)n2)CC1	10.1016/j.ejmech.2017.09.035
	10860228	116335	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338001	116335	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0341047
	10860228	116335	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338001	116335	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0502081
	3840250	136094	16	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	5	2.4	Cc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	CHEMBL3739776	136094	16	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	5	2.4	Cc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	56851447	71281	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1923026	71281	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1963433	71281	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	90645157	111692	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1Cl	10.1016/j.ejmech.2014.06.032
	CHEMBL3290584	111692	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1Cl	10.1016/j.ejmech.2014.06.032
	2971762	192624	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL523617	192624	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	44450401	155590	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	3	2	4	2.0	O=c1[nH]c2ccc(Cl)cc2nc1NCN1CCCC1	10.1021/jm7014217
	CHEMBL406345	155590	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	3	2	4	2.0	O=c1[nH]c2ccc(Cl)cc2nc1NCN1CCCC1	10.1021/jm7014217
	2971762	192624	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL523617	192624	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	164622190	185034	0	None	-93	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4864067	185034	0	None	-93	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCCc1ccccc1	10.1016/j.ejmech.2021.113190
	54586762	61198	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	4	2	5	2.0	CN1CCN(c2cc(N)nc(NCc3ccccc3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770967	61198	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	4	2	5	2.0	CN1CCN(c2cc(N)nc(NCc3ccccc3)c2)CC1	10.1016/j.bmcl.2011.03.017
	136066109	198750	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	46231557	198750	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	CHEMBL598291	198750	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	5477514	10282	1	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	408	4	4	5	2.6	C/N=C(\N)N/N=C(/C)c1ccc2sc3ccc(/C(C)=N\N/C(N)=N/C)cc3c2c1	10.1021/jm7014777
	CHEMBL1162345	10282	1	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	408	4	4	5	2.6	C/N=C(\N)N/N=C(/C)c1ccc2sc3ccc(/C(C)=N\N/C(N)=N/C)cc3c2c1	10.1021/jm7014777
	24826083	67805	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914797	67805	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	10988959	117605	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL340740	117605	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	4906599	168097	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL436381	168097	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10988959	117605	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL340740	117605	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0341047
	4906599	168097	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL436381	168097	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0341047
	57391819	69393	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935570	69393	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)c1	10.1016/j.bmcl.2011.10.104
	53233801	61216	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770985	61216	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	10988959	117605	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL340740	117605	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	4906599	168097	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL436381	168097	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	71519674	89299	0	None	-1	5	Mouse	7.8	pKi	=	7.8	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89299	0	None	-1	5	Mouse	7.8	pKi	=	7.8	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	56835171	69397	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL1935574	69397	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	71603138	89287	0	None	32	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	2	5	1.9	CNC1CN(c2nc(N)nc3cc(C(C)(C)C)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376792	89287	0	None	32	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	2	5	1.9	CNC1CN(c2nc(N)nc3cc(C(C)(C)C)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25128822	186898	0	None	-10	5	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	186898	0	None	-10	5	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	44129214	197522	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	538	7	2	7	3.7	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Br)c(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL589782	197522	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	538	7	2	7	3.7	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Br)c(Cl)cc3n2)CC1	10.1021/jm901379s
	136192874	56189	0	None	-1	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56189	0	None	-1	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25147772	172837	4	None	1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	172837	4	None	1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	136192874	56189	0	None	-1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56189	0	None	-1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1204	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1247	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1375	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	774	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	CHEMBL90	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	DB05381	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1223	940	32	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	940	32	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	940	32	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1204	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1247	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1375	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	774	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL90	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	DB05381	1901	114	None	-3	23	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1278	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	1279	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	4908365	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	CHEMBL129198	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	10967174	19242	3	None	-2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129540	19242	3	None	-2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70681963	75335	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	2	1	2	2.9	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047470	75335	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	2	1	2	2.9	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	4906599	168097	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL436381	168097	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1278	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	1279	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	4908365	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	CHEMBL129198	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	3035746	187597	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL1237146	187597	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL498770	187597	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	45269820	193679	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202326	193679	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL551790	193679	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1278	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	1279	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	4908365	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	CHEMBL129198	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	155491015	175921	3	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	175921	3	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	175921	3	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	25129525	187000	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1039/C2MD20212C
	CHEMBL494648	187000	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1039/C2MD20212C
	46228125	198305	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595215	198305	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890020	7287	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	8	3	4	4.5	Cc1cc(OCCCNCC2CCCCN2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086327	7287	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	8	3	4	4.5	Cc1cc(OCCCNCC2CCCCN2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	42641407	197705	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(Cl)cc(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL591013	197705	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(Cl)cc(Cl)cc3n2)CC1	10.1021/jm901379s
	127036981	136925	0	None	-1	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753621	136925	0	None	-1	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	135570464	178519	3	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	1	5	1.7	CN1CCN(c2nc(O)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL471712	178519	3	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	1	5	1.7	CN1CCN(c2nc(O)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354297	58578	0	None	-5	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	289	6	1	6	0.4	CN1CCN(Cc2cn(CCCc3c[nH]cn3)nn2)CC1	10.1021/jm1013488
	CHEMBL1688943	58578	0	None	-5	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	289	6	1	6	0.4	CN1CCN(Cc2cn(CCCc3c[nH]cn3)nn2)CC1	10.1021/jm1013488
	46890074	6525	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	403	8	1	3	6.2	Cc1cc(OCCCCCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083160	6525	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	403	8	1	3	6.2	Cc1cc(OCCCCCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	10107667	62835	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774599	62835	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789800	62835	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	25178755	176300	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCC[C@H]3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL461537	176300	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCC[C@H]3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	24759518	178729	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL473302	178729	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1267	3744	43	None	-33	25	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	118720439	115419	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	246	2	2	4	2.2	Nc1nc(C2CCCC2)cc(C2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354817	115419	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	246	2	2	4	2.2	Nc1nc(C2CCCC2)cc(C2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	24853527	166873	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	4	0	6	3.2	CN1CCN(c2nc3ccccc3nc2Oc2cccc(N(C)C)c2)CC1	10.1021/jm7014217
	CHEMBL429691	166873	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	4	0	6	3.2	CN1CCN(c2nc3ccccc3nc2Oc2cccc(N(C)C)c2)CC1	10.1021/jm7014217
	10107667	62835	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774599	62835	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789800	62835	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	10760516	62842	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774598	62842	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789829	62842	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	127050431	139785	0	None	4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	139785	0	None	4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	10760516	62842	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774598	62842	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789829	62842	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	57391034	67802	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914794	67802	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	57396299	67767	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	1	1	6	2.0	CN1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914758	67767	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	1	1	6	2.0	CN1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	57396300	67784	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	305	1	2	6	1.6	Nc1nc(N2CCNCC2)c2oc3ccc(F)c(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914775	67784	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	305	1	2	6	1.6	Nc1nc(N2CCNCC2)c2oc3ccc(F)c(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	46228161	198218	12	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	233	1	2	6	-0.3	CN1CCN(c2nc(N)nc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594694	198218	12	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	233	1	2	6	-0.3	CN1CCN(c2nc(N)nc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	1223	940	32	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	10023327	168450	0	None	-144	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168450	0	None	-144	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11615601	74670	0	None	-10	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	1	2	3.0	c1nc(CCCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203390	74670	0	None	-10	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	1	2	3.0	c1nc(CCCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	11600849	140014	0	None	-31	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL381074	140014	0	None	-31	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	66572108	75288	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3c(O)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047266	75288	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3c(O)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	9965450	75294	3	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3ccc(Cl)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047272	75294	3	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3ccc(Cl)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	45268970	194509	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202325	194509	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL561369	194509	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	45267238	194556	0	None	1	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	345	7	4	2	3.5	N=C(NC=C(c1ccccc1)c1ccccc1)NCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL561651	194556	0	None	1	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	345	7	4	2	3.5	N=C(NC=C(c1ccccc1)c1ccccc1)NCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1901	114	None	-3	23	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	127050019	140234	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	233	2	2	5	0.3	CN[C@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1039/C6MD00108D
	CHEMBL3818355	140234	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	233	2	2	5	0.3	CN[C@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1039/C6MD00108D
	1194	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	23786499	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	CHEMBL26116	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	127035835	136761	0	None	1	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752270	136761	0	None	1	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	70690368	75326	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	1	1	2	2.3	Cc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047461	75326	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	1	1	2	2.3	Cc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24854707	95848	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	338	3	0	5	3.3	CN1CCN(c2nc3ccccc3nc2Oc2ccc(F)cc2)CC1	10.1021/jm7014217
	CHEMBL261222	95848	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	338	3	0	5	3.3	CN1CCN(c2nc3ccccc3nc2Oc2ccc(F)cc2)CC1	10.1021/jm7014217
	57390224	67535	0	None	-776	3	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	503	8	1	5	4.7	COc1ccc(CN(CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910379	67535	0	None	-776	3	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	503	8	1	5	4.7	COc1ccc(CN(CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	4905344	35109	9	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2cc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL1437043	35109	9	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2cc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1016/j.ejmech.2012.06.016
	70690367	75324	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	3	2.0	COc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047459	75324	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	3	2.0	COc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	59777309	109665	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.3	CN[C@@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1021/jm401727m
	CHEMBL3236581	109665	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.3	CN[C@@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1021/jm401727m
	57400777	67867	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915345	67867	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	56835171	69397	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69397	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	3035746	187597	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL1237146	187597	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL498770	187597	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	53378928	115414	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	289	4	2	5	1.4	CN[C@@H]1CCN(c2cc(CCC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354812	115414	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	289	4	2	5	1.4	CN[C@@H]1CCN(c2cc(CCC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	44547564	116615	2	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	2.2	CN1CCN(c2ccnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393531	116615	2	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	2.2	CN1CCN(c2ccnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	57400777	67867	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915345	67867	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400738	67842	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.5	Nc1nc(N2CC[C@@H](N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915049	67842	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.5	Nc1nc(N2CC[C@@H](N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	56851445	71297	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923025	71297	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963551	71297	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	71603194	89292	0	None	35	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	3	2	5	1.6	CCc1ccc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376797	89292	0	None	35	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	3	2	5	1.6	CCc1ccc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	45257133	198222	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	198222	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	155540438	171974	0	None	7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4483783	171974	0	None	7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	57394625	67975	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	325	7	2	6	1.7	COc1ccccc1Cn1ccc(NCCc2cnc[nH]2)nc1=O	10.1016/j.bmcl.2011.07.114
	CHEMBL1916493	67975	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	325	7	2	6	1.7	COc1ccccc1Cn1ccc(NCCc2cnc[nH]2)nc1=O	10.1016/j.bmcl.2011.07.114
	124087	1362	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	7157	1362	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	814	1362	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	CHEMBL1172	1362	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	DB00967	1362	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	54587791	61214	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770983	61214	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	10515050	6172	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1081541	6172	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL350478	6172	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	73355143	91438	0	None	-22	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	323	8	2	4	2.8	O=C(NCCOc1ccccc1Cl)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL2413362	91438	0	None	-22	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	323	8	2	4	2.8	O=C(NCCOc1ccccc1Cl)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	56678756	65298	3	None	-	1	Human	5.8	pKi	=	5.8	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4	ChEMBL	391	5	1	4	4.2	CCC(=O)N[C@@H]1CC[C@H](C(=O)N(C)c2ccc(-c3nc4ccccc4o3)cc2)C1	nan
	CHEMBL1834199	65298	3	None	-	1	Human	5.8	pKi	=	5.8	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4	ChEMBL	391	5	1	4	4.2	CCC(=O)N[C@@H]1CC[C@H](C(=O)N(C)c2ccc(-c3nc4ccccc4o3)cc2)C1	nan
	137634349	155474	0	None	-741	2	Human	4.8	pKi	=	4.8	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	6	0	3	4.7	CC(=O)c1ccc(OCc2ccc(CN3CCCC(C)C3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4062122	155474	0	None	-741	2	Human	4.8	pKi	=	4.8	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	6	0	3	4.7	CC(=O)c1ccc(OCc2ccc(CN3CCCC(C)C3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	164626370	185891	0	None	-14	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	6	4	4	1.8	CCCCCCNC(=O)/N=C(\N)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	CHEMBL4876799	185891	0	None	-14	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	6	4	4	1.8	CCCCCCNC(=O)/N=C(\N)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	155568977	175578	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	281	6	3	3	2.6	c1ccc2[nH]c(CNCC[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	CHEMBL4592695	175578	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	281	6	3	3	2.6	c1ccc2[nH]c(CNCC[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	24854706	95532	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)c(Cl)c2)CC1	10.1021/jm7014217
	CHEMBL259536	95532	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)c(Cl)c2)CC1	10.1021/jm7014217
	1278	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1279	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	4908365	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	CHEMBL129198	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	46929554	16575	0	None	11	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	349	5	1	3	4.7	FC(F)(F)c1ccc(SCC(c2ccncc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243367	16575	0	None	11	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	349	5	1	3	4.7	FC(F)(F)c1ccc(SCC(c2ccncc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	4060730	19183	51	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129199	19183	51	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	49841608	69388	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935565	69388	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	1278	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	1279	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	4908365	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	CHEMBL129198	2119	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	4060730	19183	51	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129199	19183	51	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	45379805	197747	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	7	1.9	CN1CCN(c2nc(NCCS(=O)(=O)N3CCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591248	197747	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	7	1.9	CN1CCN(c2nc(NCCS(=O)(=O)N3CCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	164585473	183979	0	None	-3	5	Human	5.8	pKi	=	5.8	Binding	Binding affinity to H4 receptor (unknown origin)Binding affinity to H4 receptor (unknown origin)	ChEMBL	503	5	1	2	7.2	O=CN1CCC(c2ccc(-c3cc(C(=O)O)cc4cc(-c5ccc(C(F)(F)F)cc5)ccc34)cc2)CC1	10.1021/acs.jmedchem.1c00164
	CHEMBL4848380	183979	0	None	-3	5	Human	5.8	pKi	=	5.8	Binding	Binding affinity to H4 receptor (unknown origin)Binding affinity to H4 receptor (unknown origin)	ChEMBL	503	5	1	2	7.2	O=CN1CCC(c2ccc(-c3cc(C(=O)O)cc4cc(-c5ccc(C(F)(F)F)cc5)ccc34)cc2)CC1	10.1021/acs.jmedchem.1c00164
	71519177	86057	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314760	86057	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	112936262	136752	1	None	3	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2nc(-c3ccccc3)cc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752195	136752	1	None	3	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2nc(-c3ccccc3)cc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	1403136	179927	7	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL475331	179927	7	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm901379s
	164611143	184133	0	None	-75	4	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	414	6	4	4	2.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(F)(F)F	10.1016/j.ejmech.2021.113190
	CHEMBL4850448	184133	0	None	-75	4	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	414	6	4	4	2.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(F)(F)F	10.1016/j.ejmech.2021.113190
	137646744	157278	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	391	10	1	5	3.0	O=C(CCNC(=O)C(Cc1ccccc1)Cn1ccnc1)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4083336	157278	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	391	10	1	5	3.0	O=C(CCNC(=O)C(Cc1ccccc1)Cn1ccnc1)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	56835171	69397	0	None	-10	9	Rat	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from rat H4RDisplacement of [3H]histamine from rat H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69397	0	None	-10	9	Rat	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from rat H4RDisplacement of [3H]histamine from rat H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	90645149	111682	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1cccc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290574	111682	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1cccc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	46890021	7288	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.8	Cc1cc(OCCCNCC2CCCCN2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086328	7288	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.8	Cc1cc(OCCCNCC2CCCCN2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	118725541	116612	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	1	4	2.0	CCCCNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393528	116612	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	1	4	2.0	CCCCNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	16082858	79682	0	None	-8	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL213133	79682	0	None	-8	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	45379809	197708	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	420	5	1	8	2.1	CN1CCN(c2nc(NCCN3C(=O)CSC3=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591027	197708	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	420	5	1	8	2.1	CN1CCN(c2nc(NCCN3C(=O)CSC3=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	135398737	944	89	None	-57	91	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	944	89	None	-57	91	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	944	89	None	-57	91	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	944	89	None	-57	91	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	944	89	None	-57	91	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	10575070	67539	0	None	-79	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	439	7	1	3	4.7	O=C(c1cc2ccccc2[nH]1)N1CCN(CCOC(c2ccccc2)c2ccccc2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910383	67539	0	None	-79	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	439	7	1	3	4.7	O=C(c1cc2ccccc2[nH]1)N1CCN(CCOC(c2ccccc2)c2ccccc2)CC1	10.1016/j.bmcl.2011.09.001
	164622330	185280	0	None	-724	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4867962	185280	0	None	-724	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	1267	3744	43	None	-33	25	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	3035905	3744	43	None	-33	25	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	CHEMBL260374	3744	43	None	-33	25	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	122193098	123399	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628045	123399	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645162	111697	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	324	2	1	6	1.3	CN1CCN(c2nc(N)nc(-c3cc(F)c(F)c(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290589	111697	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	324	2	1	6	1.3	CN1CCN(c2nc(N)nc(-c3cc(F)c(F)c(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	24745507	67878	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915538	67878	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	45257133	198222	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	198222	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	24826165	67772	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914763	67772	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	1278	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	1279	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	4908365	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	CHEMBL129198	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	3032915	15321	20	None	-1	6	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1021/jm0341047
	CHEMBL12160	15321	20	None	-1	6	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1021/jm0341047
	127052294	139772	0	None	5	4	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	139772	0	None	5	4	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	57394550	67774	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	2	1	7	2.2	COc1ccc2c(c1)sc1c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914765	67774	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	2	1	7	2.2	COc1ccc2c(c1)sc1c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.08.014
	51354009	58575	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cc2cn(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688940	58575	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cc2cn(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	156011119	176548	0	None	-3	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	841	18	4	6	8.7	Cc1cc(OCCCNC(=O)CCCCCNC(=O)COc2ccc(/C=C/C3=[N+]4C(=Cc5ccc(-c6ccc[nH]6)n5[B-]4(F)F)C=C3)cc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4633688	176548	0	None	-3	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	841	18	4	6	8.7	Cc1cc(OCCCNC(=O)CCCCCNC(=O)COc2ccc(/C=C/C3=[N+]4C(=Cc5ccc(-c6ccc[nH]6)n5[B-]4(F)F)C=C3)cc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	51354296	58577	0	None	-4	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	274	6	1	5	1.6	c1nc(CCCn2cc(CN3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688942	58577	0	None	-4	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	274	6	1	5	1.6	c1nc(CCCn2cc(CN3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	1236	2036	23	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	1237	2036	23	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	156615	2036	23	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	CHEMBL268229	2036	23	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	756701	198135	29	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	218	1	1	5	0.1	CN1CCN(c2ncnc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594152	198135	29	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	218	1	1	5	0.1	CN1CCN(c2ncnc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	63868328	173679	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4549015	173679	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	58227381	176042	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4513989	176042	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597817	176042	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25128822	186898	0	None	-10	5	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL494093	186898	0	None	-10	5	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	25179124	173661	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm800876b
	CHEMBL454883	173661	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm800876b
	25179122	192670	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL523941	192670	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179124	173661	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm901379s
	CHEMBL454883	173661	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm901379s
	25179122	192670	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL523941	192670	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1265	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	6422124	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	CHEMBL14638	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	24994634	2111	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2111	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2111	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2111	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2111	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2111	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL4520788	212225	6	None	-89	25	Human	5.7	pKi	=	5.7	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000743a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000743a HRH4	ChEMBL		None	None	None		CNCc1ccccc1-c1csc([C@H](C)Nc2nc(C)nc3cc(OC)c(OC)cc23)c1	nan
	54581937	61194	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	3	1	5	1.3	CC(C)N(C)c1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770963	61194	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	3	1	5	1.3	CC(C)N(C)c1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	137646939	157196	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	335	5	3	4	3.6	CC(C)(CN=C(c1ccccc1O)c1ccccc1O)c1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL4082297	157196	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	335	5	3	4	3.6	CC(C)(CN=C(c1ccccc1O)c1ccccc1O)c1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	9878733	116344	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL338051	116344	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9878733	116344	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338051	116344	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	49841610	69389	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3cc(-c4ccccc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935566	69389	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3cc(-c4ccccc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	44547127	115418	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	44547127	115418	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115418	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354816	115418	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	24739219	67711	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	289	1	2	6	1.8	Nc1nc(N2CC(N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914546	67711	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	289	1	2	6	1.8	Nc1nc(N2CC(N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	24826265	67770	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.3	CN1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914761	67770	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.3	CN1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	9992865	113928	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL333220	113928	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	9878733	116344	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338051	116344	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	2063135	8293	9	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093180	8293	9	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	44129213	197702	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)c(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL590995	197702	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)c(Cl)cc3n2)CC1	10.1021/jm901379s
	71525681	89298	0	None	-2	6	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89298	0	None	-2	6	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	136141537	56187	1	None	2	3	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56187	1	None	2	3	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	136030655	56188	0	None	-2	3	Rat	7.7	pKi	=	7.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56188	0	None	-2	3	Rat	7.7	pKi	=	7.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1278	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1279	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	4908365	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL129198	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1251	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	3035842	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL18661	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1251	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	3035842	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	CHEMBL18661	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	1251	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	3035842	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	CHEMBL18661	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	11673377	138732	0	None	-1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	263	7	2	2	3.2	Clc1ccc(CNCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	CHEMBL379027	138732	0	None	-1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	263	7	2	2	3.2	Clc1ccc(CNCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	25129523	186704	40	None	1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	186704	40	None	1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	66572114	75298	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047276	75298	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70690369	75338	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	1	2	2	2.0	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047473	75338	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	1	2	2	2.0	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	9878733	116344	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL338051	116344	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1204	1901	114	None	-3	23	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1247	1901	114	None	-3	23	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1375	1901	114	None	-3	23	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	774	1901	114	None	-3	23	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	CHEMBL90	1901	114	None	-3	23	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	DB05381	1901	114	None	-3	23	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1278	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2119	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	11846298	138751	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	CHEMBL379102	138751	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	155538989	175755	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4513421	175755	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595438	175755	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	164608815	183872	0	None	-47	6	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	7	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4846638	183872	0	None	-47	6	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	7	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1016/j.ejmech.2021.113190
	164611342	184505	0	None	-416	6	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	346	6	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856005	184505	0	None	-416	6	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	346	6	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1016/j.ejmech.2021.113190
	155513612	175746	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156015838	177671	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4640263	177671	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650622	177671	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	127036982	136942	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	525	6	1	6	5.9	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3c2CCc2ccccc2-3)cc1-c1ccc(Cl)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753784	136942	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	525	6	1	6	5.9	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3c2CCc2ccccc2-3)cc1-c1ccc(Cl)cc1	10.1016/j.bmcl.2015.12.035
	57393769	67828	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	281	1	2	6	1.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915030	67828	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	281	1	2	6	1.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	11688307	69560	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938968	69560	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136141537	56187	1	None	-10	3	Rat	6.7	pKi	=	6.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56187	1	None	-10	3	Rat	6.7	pKi	=	6.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	44395364	66565	0	None	15	2	Human	6.7	pKi	=	6.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	276	2	1	2	3.3	CN1CCC(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186456	66565	0	None	15	2	Human	6.7	pKi	=	6.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	276	2	1	2	3.3	CN1CCC(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	10037650	5138	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106158	5138	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	135398737	944	89	None	-57	91	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	38	944	89	None	-57	91	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	722	944	89	None	-57	91	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	CHEMBL42	944	89	None	-57	91	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	DB00363	944	89	None	-57	91	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	11572015	74680	0	None	-50	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	9	2	2	2.5	CCCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL203441	74680	0	None	-50	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	9	2	2	2.5	CCCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11600780	75696	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	2	2	1.7	CC(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205732	75696	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	2	2	1.7	CC(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	1408942	64792	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	254	2	0	4	1.9	CN1CCN(c2cc(-c3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824046	64792	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	254	2	0	4	1.9	CN1CCN(c2cc(-c3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.06.123
	4060730	19183	51	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19183	51	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	25129527	186831	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	186831	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	66573462	75318	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	2	2	2	2.2	CNC1CN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047453	75318	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	2	2	2	2.2	CNC1CN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	767246	95571	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	242	1	0	4	1.7	Cc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL259745	95571	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	242	1	0	4	1.7	Cc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	155550732	176055	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4540849	176055	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4597895	176055	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	11313837	2464	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1254	2464	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL175782	2464	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	11313837	2464	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	1254	2464	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	CHEMBL175782	2464	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	71681480	89231	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	307	6	3	7	2.0	Nc1nc(NCCNc2ccncn2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375761	89231	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	307	6	3	7	2.0	Nc1nc(NCCNc2ccncn2)cc(-c2ccccc2)n1	10.1021/jm301886t
	57392460	69523	0	None	14	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL1938843	69523	0	None	14	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572104	75284	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ccncc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047262	75284	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ccncc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572112	75296	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3ccc([N+](=O)[O-])cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047274	75296	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3ccc([N+](=O)[O-])cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	44395361	66606	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	293	1	2	3	2.0	CN1CCN(C(=O)Nc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186640	66606	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	293	1	2	3	2.0	CN1CCN(C(=O)Nc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	10879463	5218	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106570	5218	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10945113	109141	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL322256	109141	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	127035836	136993	0	None	-5	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	4	1	5	3.9	Cc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3754176	136993	0	None	-5	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	4	1	5	3.9	Cc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	164618283	183961	0	None	-537	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	5	2.4	COc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848026	183961	0	None	-537	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	5	2.4	COc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	53233805	61195	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770964	61195	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	54585781	61232	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771000	61232	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	118720442	115421	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	3	5	1.4	Nc1cc(N2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354820	115421	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	3	5	1.4	Nc1cc(N2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	44547268	116610	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393526	116610	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227255	116623	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393539	116623	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	58227252	116628	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393544	116628	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	25112105	8079	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1091875	8079	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737949	67790	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914782	67790	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	24825996	67796	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914788	67796	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	59777341	109659	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	275	3	2	5	1.9	CN[C@@H]1CCN(c2cc(C3CCCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236576	109659	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	275	3	2	5	1.9	CN[C@@H]1CCN(c2cc(C3CCCCC3)nc(N)n2)C1	10.1021/jm401727m
	1278	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	24745335	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	24745335	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	53257796	109658	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236575	109658	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	24738269	67708	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.1	Nc1nc(N2CC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914543	67708	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.1	Nc1nc(N2CC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	1223	940	32	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	940	32	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	940	32	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	44231735	7703	0	None	-1	4	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089390	7703	0	None	-1	4	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	44462956	5523	0	None	2	2	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL1077276	5523	0	None	2	2	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	1278	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1278	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2119	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	137657169	159040	0	None	3	2	Human	8.6	pKi	=	8.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	8	1	4	4.1	O=[N+]([O-])c1cccc(C(OCCCc2c[nH]cn2)c2ccccc2)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4102970	159040	0	None	3	2	Human	8.6	pKi	=	8.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	8	1	4	4.1	O=[N+]([O-])c1cccc(C(OCCCc2c[nH]cn2)c2ccccc2)c1	10.1016/j.ejmech.2016.09.074
	59777258	109644	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236561	109644	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	25130914	186671	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL492677	186671	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	46890547	6438	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082772	6438	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	10446295	2101	29	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	1277	2101	29	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	CHEMBL185951	2101	29	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	68081305	109633	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	1	1	2	1.3	CN1CCN(C(=O)c2cc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	CHEMBL3236550	109633	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	1	1	2	1.3	CN1CCN(C(=O)c2cc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	24798144	109636	2	None	-19	3	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109636	2	None	-19	3	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	24738103	67788	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(OC)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914780	67788	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(OC)c23)C1	10.1016/j.bmcl.2011.08.014
	24745335	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	16362	3076	67	None	-562	29	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	2172	3076	67	None	-562	29	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	90	3076	67	None	-562	29	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	CHEMBL1423	3076	67	None	-562	29	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	DB01100	3076	67	None	-562	29	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	9904236	13962	16	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL1197564	13962	16	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46890589	6944	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	7	1	4	4.8	Cc1cc(OCCCN2CCCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084809	6944	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	7	1	4	4.8	Cc1cc(OCCCN2CCCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57396382	67977	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	9	2	7	3.8	COCc1ccc(CN2CC[C@H](Nc3cc(NCCC(C)(C)C)ncn3)C2)o1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916495	67977	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	9	2	7	3.8	COCc1ccc(CN2CC[C@H](Nc3cc(NCCC(C)(C)C)ncn3)C2)o1	10.1016/j.bmcl.2011.07.114
	57391122	67978	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	7	3	6	3.9	CC(C)(C)CCNc1cc(N[C@H]2CCN(Cc3cccc(F)c3O)C2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916496	67978	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	7	3	6	3.9	CC(C)(C)CCNc1cc(N[C@H]2CCN(Cc3cccc(F)c3O)C2)ncn1	10.1016/j.bmcl.2011.07.114
	57394628	67983	1	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	4	2	5	1.7	CC(C)(C)CCNc1cc(N2CCNCC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916500	67983	1	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	4	2	5	1.7	CC(C)(C)CCNc1cc(N2CCNCC2)ncn1	10.1016/j.bmcl.2011.07.114
	57397738	69521	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	245	1	0	5	0.1	CN1CCN(C(=O)c2cn3ccncc3n2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938841	69521	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	245	1	0	5	0.1	CN1CCN(C(=O)c2cn3ccncc3n2)CC1	10.1016/j.bmcl.2011.11.098
	10399535	117937	3	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	2	2	1.6	CC1CN(C(=O)c2cc3ccccc3[nH]2)CCN1	10.1021/jm0341047
	CHEMBL341087	117937	3	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	2	2	1.6	CC1CN(C(=O)c2cc3ccccc3[nH]2)CCN1	10.1021/jm0341047
	90645147	111684	0	None	13	3	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290576	111684	0	None	13	3	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	155567108	176062	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4586599	176062	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597946	176062	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	155536509	175898	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4473753	175898	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596582	175898	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	4687476	95864	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL261320	95864	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	9928073	201954	0	None	-67	3	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	343	0	0	4	4.1	CN1CCN(C2=Nc3cc(Cl)ccc3Sc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL67284	201954	0	None	-67	3	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	343	0	0	4	4.1	CN1CCN(C2=Nc3cc(Cl)ccc3Sc3ccccc32)CC1	10.1021/jm051008s
	90654501	109820	0	None	-21877	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	314	6	0	5	3.1	Cc1nnc(C)n1-c1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	CHEMBL3238444	109820	0	None	-21877	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	314	6	0	5	3.1	Cc1nnc(C)n1-c1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	4687476	95864	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1021/jm7014217
	CHEMBL261320	95864	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1021/jm7014217
	10879463	5218	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106570	5218	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	46228132	200459	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL609161	200459	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	46228071	198494	15	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL596562	198494	15	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	51354870	58596	0	None	-26	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	305	6	0	5	2.0	CN1CCN(CCCn2cc(CC3CCCCC3)nn2)CC1	10.1021/jm1013488
	CHEMBL1688981	58596	0	None	-26	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	305	6	0	5	2.0	CN1CCN(CCCn2cc(CC3CCCCC3)nn2)CC1	10.1021/jm1013488
	57391928	69566	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4ccc(Cl)nc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938973	69566	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4ccc(Cl)nc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	23650960	148597	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(F)ccc2[nH]1	nan
	CHEMBL3943309	148597	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(F)ccc2[nH]1	nan
	42624149	109651	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3236568	109651	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1016/j.bmcl.2014.10.013
	25218146	116605	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	2	0	4	2.6	CN1CCN(c2ccnc(-c3ccsc3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393521	116605	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	2	0	4	2.6	CN1CCN(c2ccnc(-c3ccsc3)c2)CC1	10.1016/j.bmcl.2014.12.027
	42624149	109651	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	109651	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	53258370	109652	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236569	109652	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	57394548	67756	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@@H]3CCCN[C@@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914747	67756	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@@H]3CCCN[C@@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	57396298	67761	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	1	1	6	1.8	CN1CCN(c2nc(N)nc3c2oc2cccc(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914752	67761	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	1	1	6	1.8	CN1CCN(c2nc(N)nc3c2oc2cccc(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	44628629	67776	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	269	1	2	6	1.4	Nc1nc(N2CCNCC2)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914767	67776	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	269	1	2	6	1.4	Nc1nc(N2CCNCC2)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	11630326	72226	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199231	72226	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	51354582	58590	0	None	2	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.3	CC(c1cn(CCCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	CHEMBL1688974	58590	0	None	2	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.3	CC(c1cn(CCCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	71603195	89293	0	None	8	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	3	2	5	1.9	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376798	89293	0	None	8	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	3	2	5	1.9	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	1251	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	3035842	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	CHEMBL18661	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	1530	2151	44	None	-21379	20	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	3827	2151	44	None	-21379	20	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	7206	2151	44	None	-21379	20	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	CHEMBL534	2151	44	None	-21379	20	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	DB00920	2151	44	None	-21379	20	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	57399107	67872	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1021/acs.jmedchem.5b00516
	CHEMBL1915532	67872	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1021/acs.jmedchem.5b00516
	57399107	67872	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915532	67872	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.125
	57399107	67872	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915532	67872	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.114
	57395619	67873	13	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915533	67873	13	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	57395619	67873	13	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915533	67873	13	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	168268680	192164	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5172675	192164	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221210	192164	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	1403136	179927	7	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL475331	179927	7	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm800876b
	1204	1901	114	None	-15	23	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-15	23	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-15	23	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-15	23	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-15	23	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-15	23	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	164610824	184527	0	None	-14	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	452	8	4	4	3.6	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856200	184527	0	None	-14	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	452	8	4	4	3.6	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	10945113	109141	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL322256	109141	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	21896704	158979	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	429	9	1	7	2.7	CC(=O)SCC(Cc1ccc2c(c1)OCO2)C(=O)NCC(=O)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4102243	158979	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	429	9	1	7	2.7	CC(=O)SCC(Cc1ccc2c(c1)OCO2)C(=O)NCC(=O)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	46912441	10801	0	None	-117	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	309	3	1	2	3.9	FC(F)(F)c1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1172754	10801	0	None	-117	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	309	3	1	2	3.9	FC(F)(F)c1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	118725538	116606	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	3	1	4	2.6	CN[C@@H]1CCN(c2ccnc(-c3ccsc3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393522	116606	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	3	1	4	2.6	CN[C@@H]1CCN(c2ccnc(-c3ccsc3)c2)C1	10.1016/j.bmcl.2014.12.027
	1204	1901	114	None	-3	23	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	155567030	175843	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4586133	175843	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4596108	175843	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	24854708	155182	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL405416	155182	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	51354587	58595	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	0	5	2.7	c1cn(CCCn2cc(CC3CCCCC3)nn2)cn1	10.1021/jm1013488
	CHEMBL1688979	58595	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	0	5	2.7	c1cn(CCCn2cc(CC3CCCCC3)nn2)cn1	10.1021/jm1013488
	10702196	108081	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL320279	108081	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	136016345	10286	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	377	5	4	6	2.1	CCOC(=O)c1ccc(NC2=CC(=O)/C(=N\NC(=N)N)c3ccccc32)cc1	10.1021/jm7014777
	CHEMBL1162352	10286	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	377	5	4	6	2.1	CCOC(=O)c1ccc(NC2=CC(=O)/C(=N\NC(=N)N)c3ccccc32)cc1	10.1021/jm7014777
	127052293	139708	1	None	34	4	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139708	1	None	34	4	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1251	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	3035842	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	CHEMBL18661	1983	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	11258459	83814	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	CHEMBL220933	83814	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	46228082	200692	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	340	5	2	6	2.5	CC(C)c1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL610626	200692	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	340	5	2	6	2.5	CC(C)c1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	49841721	69381	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	CHEMBL1935558	69381	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	25178551	173659	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454882	173659	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm800876b
	25178551	173659	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454882	173659	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm901379s
	45379807	197828	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	6	3.5	CN1CCN(c2nc(NCCC(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591970	197828	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	6	3.5	CN1CCN(c2nc(NCCC(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10037650	5138	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106158	5138	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	122194624	123502	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccccc1/C=C/c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629827	123502	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccccc1/C=C/c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2015.08.014
	57400700	67536	0	None	-3090	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	470	8	1	6	3.4	COc1ccc(CN(CCN2CCN(C(=O)c3nc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910380	67536	0	None	-3090	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	470	8	1	6	3.4	COc1ccc(CN(CCN2CCN(C(=O)c3nc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	164610421	184678	0	None	-7	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	332	5	4	4	2.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)Nc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4858567	184678	0	None	-7	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	332	5	4	4	2.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)Nc1ccccc1	10.1016/j.ejmech.2021.113190
	46890120	6459	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	416	6	1	4	5.2	Cc1cc(OCCCN2Cc3cccnc3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082851	6459	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	416	6	1	4	5.2	Cc1cc(OCCCN2Cc3cccnc3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	56851604	71285	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923028	71285	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963460	71285	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	24853663	95585	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	272	3	0	5	1.8	CCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL259839	95585	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	272	3	0	5	1.8	CCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	24278976	95804	56	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2nc3ccccc3nc2Cl)CC1	10.1021/jm7014217
	CHEMBL261010	95804	56	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2nc3ccccc3nc2Cl)CC1	10.1021/jm7014217
	24854704	95688	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	CHEMBL260456	95688	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	10923241	18972	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128958	18972	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10923241	18972	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128958	18972	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0341047
	56835171	69397	0	None	-1	9	Mouse	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from mouse H4RDisplacement of [3H]histamine from mouse H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69397	0	None	-1	9	Mouse	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from mouse H4RDisplacement of [3H]histamine from mouse H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	11687917	14796	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	CHEMBL120854	14796	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	10923241	18972	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128958	18972	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	45257131	198226	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	198226	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	57403350	67980	0	None	35	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	3	1	5	1.7	CN1CCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916498	67980	0	None	35	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	3	1	5	1.7	CN1CCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	24804058	143565	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	424	7	2	5	5.1	Cc1c(F)ccc2[nH]c(-c3cnc(NCCCC4CCN(C)CC4)nc3C(C)C)nc12	nan
	CHEMBL3903520	143565	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	424	7	2	5	5.1	Cc1c(F)ccc2[nH]c(-c3cnc(NCCCC4CCN(C)CC4)nc3C(C)C)nc12	nan
	126963284	144241	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL3909134	144241	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46890675	7162	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	1	4	4.4	Cc1cc(OCCCN2CC3CCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085803	7162	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	1	4	4.4	Cc1cc(OCCCN2CC3CCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	49841722	69382	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3c(-c4ccc(C#N)cc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935559	69382	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3c(-c4ccc(C#N)cc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	168276867	192233	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174278	192233	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221653	192233	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	24853598	95776	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	350	4	0	6	3.2	COc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL260851	95776	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	350	4	0	6	3.2	COc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	24853596	95851	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	354	3	0	5	3.8	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	CHEMBL261225	95851	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	354	3	0	5	3.8	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	164610289	184550	0	None	-38	4	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	7	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856602	184550	0	None	-38	4	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	7	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	57399827	67984	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916501	67984	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	90645155	111690	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290582	111690	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	90645155	111690	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL3290582	111690	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	46228114	198492	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596551	198492	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25178131	186355	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	381	5	1	5	3.7	CN1CCN(c2nc(NCCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL490072	186355	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	381	5	1	5	3.7	CN1CCN(c2nc(NCCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	90645152	111687	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	396	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(I)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290579	111687	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	396	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(I)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	49818718	69387	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935564	69387	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	54582867	61230	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770999	61230	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	25147771	173666	0	None	1	3	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	173666	0	None	1	3	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	11440060	205303	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	368	8	1	2	5.4	c1ccc(C(OCCCc2c[nH]cn2)(c2ccccc2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL91576	205303	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	368	8	1	2	5.4	c1ccc(C(OCCCc2c[nH]cn2)(c2ccccc2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	127051301	139670	0	None	5	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139670	0	None	5	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227216	116638	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393554	116638	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	550981	68689	12	None	2	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	280	5	2	3	2.9	OC(CNC1CC1)Cn1c2ccccc2c2ccccc21	10.1021/jm2011589
	CHEMBL1923533	68689	12	None	2	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	280	5	2	3	2.9	OC(CNC1CC1)Cn1c2ccccc2c2ccccc21	10.1021/jm2011589
	24804056	150119	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	420	8	2	5	5.1	CCCc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(C)ccc2[nH]1	nan
	CHEMBL3955597	150119	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	420	8	2	5	5.1	CCCc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(C)ccc2[nH]1	nan
	71461658	78647	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	4	0.5	CN1CCN(C(=O)c2nc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL2113162	78647	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	4	0.5	CN1CCN(C(=O)c2nc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	4024	1984	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	9989505	1984	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	CHEMBL82298	1984	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	122193101	123402	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.4	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C(F)(F)F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628048	123402	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.4	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C(F)(F)F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	155546701	173083	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	2	1	6	0.8	CN1CCN(c2nc(N)nc(CC(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4534504	173083	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	2	1	6	0.8	CN1CCN(c2nc(N)nc(CC(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	24798596	198185	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594491	198185	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	21193534	11160	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL1179089	11160	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL59679	11160	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	54587789	61207	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(NCCO)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770976	61207	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(NCCO)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	45257131	198226	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	198226	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	90655027	109638	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236555	109638	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	66740898	109645	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	CHEMBL3236562	109645	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	24826082	67799	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914791	67799	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	11587279	124531	3	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL364400	124531	3	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	1226	1988	16	None	2	6	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	41376	1988	16	None	2	6	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	CHEMBL12608	1988	16	None	2	6	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	10105117	103718	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL309819	103718	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10466840	202776	0	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CC/N=C(\S)NCCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72424	202776	0	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CC/N=C(\S)NCCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	1204	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1247	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1375	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	774	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	CHEMBL90	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	DB05381	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	71681476	89226	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	316	8	4	8	1.5	CC(C)CCc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375756	89226	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	316	8	4	8	1.5	CC(C)CCc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	4060730	19183	51	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm301886t
	CHEMBL129199	19183	51	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm301886t
	10923241	18972	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL128958	18972	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	4060730	19183	51	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129199	19183	51	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	22660616	75295	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3ccc(O)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047273	75295	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3ccc(O)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	71525588	89300	0	None	33	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.1	CNC1CN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376805	89300	0	None	33	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.1	CNC1CN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846300	79856	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm7014217
	CHEMBL213912	79856	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm7014217
	45271470	193380	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202329	193380	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL549481	193380	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	45270644	193801	0	None	3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	257	6	4	2	1.7	N=C(NCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmc.2009.04.007
	CHEMBL552694	193801	0	None	3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	257	6	4	2	1.7	N=C(NCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmc.2009.04.007
	45271464	193831	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202328	193831	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL553455	193831	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10468601	193918	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202324	193918	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL555434	193918	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45268095	194366	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202323	194366	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560313	194366	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1247	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1375	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	774	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	CHEMBL90	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	DB05381	1901	114	None	-3	23	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1274	4001	12	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	3063228	4001	12	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	CHEMBL1196470	4001	12	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	10988959	117605	4	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL340740	117605	4	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1039/C2MD20212C
	21638785	188073	4	None	-4	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188073	4	None	-4	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	127052294	139772	0	None	5	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	139772	0	None	5	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11751419	137355	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL376268	137355	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	11751419	137355	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2006.08.117
	CHEMBL376268	137355	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2006.08.117
	57402512	67825	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915022	67825	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	46228092	198266	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	299	4	1	6	1.4	CN1CCN(c2cc(OCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594973	198266	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	299	4	1	6	1.4	CN1CCN(c2cc(OCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	4024	1984	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	9989505	1984	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL82298	1984	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	1252	1986	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	9793868	1986	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL417096	1986	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1236	2036	23	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1237	2036	23	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	156615	2036	23	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL268229	2036	23	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1252	1986	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	9793868	1986	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	CHEMBL417096	1986	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	81371	12661	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187946	12661	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	CHEMBL534494	12661	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	52128564	75281	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	0	3	2.5	CN1CCN(C(=O)c2cc3cc(Cl)ccc3o2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047259	75281	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	0	3	2.5	CN1CCN(C(=O)c2cc3cc(Cl)ccc3o2)CC1	10.1016/j.ejmech.2012.06.016
	70684084	75317	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	2	2	2.6	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047452	75317	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	2	2	2.6	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	70692528	75323	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047458	75323	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70686224	75337	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	2	2.4	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047472	75337	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	2	2.4	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	51355156	58602	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	3.1	c1nc(CCCc2cn(C3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688989	58602	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	3.1	c1nc(CCCc2cn(C3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	9990475	56183	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	CHEMBL1632408	56183	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	CHEMBL2448450	56183	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	10583206	108872	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321920	108872	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	71681640	89233	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nc(Cl)cc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375763	89233	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nc(Cl)cc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	12438303	75280	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	260	1	0	3	2.3	CN1CCN(C(=O)c2cc3ccccc3s2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047258	75280	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	260	1	0	3	2.3	CN1CCN(C(=O)c2cc3ccccc3s2)CC1	10.1016/j.ejmech.2012.06.016
	22660598	75305	1	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047440	75305	1	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24854636	155776	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	296	1	0	4	2.4	CN1CCN(c2nc3ccccc3nc2C(F)(F)F)CC1	10.1021/jm7014217
	CHEMBL406558	155776	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	296	1	0	4	2.4	CN1CCN(c2nc3ccccc3nc2C(F)(F)F)CC1	10.1021/jm7014217
	10583206	108872	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321920	108872	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	44395263	66370	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	292	2	2	3	1.7	CN1CCN(NC(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL185546	66370	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	292	2	2	3	1.7	CN1CCN(NC(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	44395336	66457	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	280	4	1	3	1.9	CN(C)CCN(C)C(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL185948	66457	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	280	4	1	3	1.9	CN(C)CCN(C)C(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	44395290	124997	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	279	4	1	2	2.5	CN(C)CCN(C)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL364629	124997	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	279	4	1	2	2.5	CN(C)CCN(C)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	15984057	139591	0	None	-35481	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	348	5	0	2	5.3	c1cc(-c2ccc(CN3CCCCC3)cc2)ccc1CN1CCCCC1	10.1016/j.bmc.2008.10.029
	CHEMBL380101	139591	0	None	-35481	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	348	5	0	2	5.3	c1cc(-c2ccc(CN3CCCCC3)cc2)ccc1CN1CCCCC1	10.1016/j.bmc.2008.10.029
	71519348	86063	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	3	2	6	1.5	Nc1nc(N2CCNCC2)c2ccn(Cc3ccccc3)c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314766	86063	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	3	2	6	1.5	Nc1nc(N2CCNCC2)c2ccn(Cc3ccccc3)c2n1	10.1016/j.bmcl.2012.10.139
	122193106	123406	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	335	3	2	6	1.9	CN1CCN(c2nc(N)nc(/C=C/c3c[nH]c4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628052	123406	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	335	3	2	6	1.9	CN1CCN(c2nc(N)nc(/C=C/c3c[nH]c4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	122193095	123396	0	None	3	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123396	0	None	3	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	54585776	61192	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(N)nc(NCCO)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770961	61192	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(N)nc(NCCO)n2)C1	10.1016/j.bmcl.2011.03.017
	24994630	109642	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	2	5	1.6	Nc1nc(C2CCCC2)cc(N2CCC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236559	109642	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	2	5	1.6	Nc1nc(C2CCCC2)cc(N2CCC[C@@H](N)C2)n1	10.1021/jm401727m
	25178933	186354	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL490071	186354	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178933	186354	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL490071	186354	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	122193103	123404	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	341	4	1	8	1.3	CN1CCN(c2nc(N)nc(/C=C/c3cccc([N+](=O)[O-])c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628050	123404	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	341	4	1	8	1.3	CN1CCN(c2nc(N)nc(/C=C/c3cccc([N+](=O)[O-])c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	10446295	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	1277	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL185951	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	10446295	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	1277	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	CHEMBL185951	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	46890627	6511	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083098	6511	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11340020	83847	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	CHEMBL221210	83847	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	136042265	69567	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938974	69567	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11340020	83847	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL221210	83847	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	11189343	141445	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	420	6	1	4	4.9	Cc1cc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)ccc1OCCCN1CCN(C)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL387342	141445	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	420	6	1	4	4.9	Cc1cc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)ccc1OCCCN1CCN(C)CC1	10.1016/j.bmcl.2006.08.117
	57394549	67757	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914748	67757	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	10446295	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2101	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	24759624	95686	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	0	5	2.0	COc1nc2cc(Cl)ccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL260444	95686	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	0	5	2.0	COc1nc2cc(Cl)ccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	25147771	173666	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	173666	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	44129211	199290	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	383	5	1	7	1.5	CN1CCN(c2nc(NCCS(C)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL601946	199290	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	383	5	1	7	1.5	CN1CCN(c2nc(NCCS(C)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11821243	18664	8	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	2	1	2	1.9	CCN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128134	18664	8	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	2	1	2	1.9	CCN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	155549319	175750	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4538671	175750	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595399	175750	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	164620704	185501	0	None	-17	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	8	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)c1ccc(C(C)C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4871482	185501	0	None	-17	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	8	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)c1ccc(C(C)C)cc1	10.1016/j.ejmech.2021.113190
	127026016	136964	0	None	-13	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753967	136964	0	None	-13	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	71518861	86070	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	5	2.1	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314774	86070	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	5	2.1	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	122193102	123403	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	321	3	1	7	1.2	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628049	123403	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	321	3	1	7	1.2	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	63868018	174804	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	208	1	1	6	-0.5	Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4574940	174804	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	208	1	1	6	-0.5	Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71518536	86067	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	3	5	0.3	Cc1cc2c(N3CCNCC3)nc(N)nc2[nH]1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314771	86067	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	3	5	0.3	Cc1cc2c(N3CCNCC3)nc(N)nc2[nH]1	10.1016/j.bmcl.2012.10.139
	57391927	69561	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938969	69561	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	3991311	136338	9	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	296	2	0	5	2.7	CCc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	CHEMBL3741986	136338	9	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	296	2	0	5	2.7	CCc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	25178556	188496	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL509961	188496	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	44563707	200911	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccc(Cl)cc3nc2OCc2ccccc2)CC1	10.1021/jm800876b
	CHEMBL612059	200911	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccc(Cl)cc3nc2OCc2ccccc2)CC1	10.1021/jm800876b
	24853595	159624	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2cccc(Cl)c2)CC1	10.1021/jm7014217
	CHEMBL410925	159624	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2cccc(Cl)c2)CC1	10.1021/jm7014217
	155535098	175812	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4471442	175812	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595908	175812	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	10782821	95775	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	332	4	0	4	3.4	CCN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260850	95775	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	332	4	0	4	3.4	CCN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	164616851	184367	0	None	-446	5	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4853772	184367	0	None	-446	5	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	10591811	158806	0	None	4	2	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	9	1	2	4.7	c1ccc(-c2ccc(CCCOCCCc3c[nH]cn3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4100315	158806	0	None	4	2	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	9	1	2	4.7	c1ccc(-c2ccc(CCCOCCCc3c[nH]cn3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	1267	3744	43	None	-33	25	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	3035905	3744	43	None	-33	25	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	CHEMBL260374	3744	43	None	-33	25	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	24804219	148031	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	378	6	2	5	4.2	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cccc2[nH]1	nan
	CHEMBL3938862	148031	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	378	6	2	5	4.2	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cccc2[nH]1	nan
	11565330	96771	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	CHEMBL268525	96771	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	1267	3744	43	None	-33	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	3035905	3744	43	None	-33	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	CHEMBL260374	3744	43	None	-33	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	71525681	89298	0	None	-3	6	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89298	0	None	-3	6	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846634	77655	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209758	77655	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	57395496	67831	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915034	67831	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	51354302	58586	0	None	1	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688970	58586	0	None	1	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25130235	187004	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187004	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187004	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187004	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	57390225	67540	0	None	-3235	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	9	2	5	4.1	COc1ccc(CN(CCN[C@H]2CCN(C(=O)c3cc4ccccc4[nH]3)C2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910384	67540	0	None	-3235	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	9	2	5	4.1	COc1ccc(CN(CCN[C@H]2CCN(C(=O)c3cc4ccccc4[nH]3)C2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	55251397	172693	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	2	1	6	0.3	CC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4525008	172693	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	2	1	6	0.3	CC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	53233803	61193	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770962	61193	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	11293460	84419	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	434	6	1	4	5.1	CN1CCN(CCCOc2ccc(-c3nc4c(ccc5ccccc54)[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL223490	84419	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	434	6	1	4	5.1	CN1CCN(CCCOc2ccc(-c3nc4c(ccc5ccccc54)[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	42624148	109657	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236574	109657	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	11846300	79856	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm800876b
	CHEMBL213912	79856	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm800876b
	11846300	79856	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79856	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	217674	178701	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL473098	178701	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	217674	178701	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL473098	178701	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	156009715	177745	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634631	177745	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651103	177745	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	45257134	198261	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	CHEMBL594949	198261	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	155539562	172274	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	304	4	1	6	1.7	CN1CCN(c2nc(N)nc(CCC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4514667	172274	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	304	4	1	6	1.7	CN1CCN(c2nc(N)nc(CCC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	10083188	140403	3	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL382276	140403	3	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	9927983	79442	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL1795042	79442	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL212212	79442	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	49841612	69391	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3cc(-c4ccc(C#N)cc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935568	69391	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3cc(-c4ccc(C#N)cc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	54587786	61201	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770970	61201	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	24825897	67768	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	1	1	6	2.2	CN1CCN(c2nc(N)nc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914759	67768	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	1	1	6	2.2	CN1CCN(c2nc(N)nc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	71519674	89299	0	None	-3	5	Rat	7.5	pKi	=	7.5	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89299	0	None	-3	5	Rat	7.5	pKi	=	7.5	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	56851610	71283	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923031	71283	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963448	71283	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	127049856	139715	0	None	2	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805431	139715	0	None	2	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	10680721	5904	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1080089	5904	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL158085	5904	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	753256	178770	10	None	2	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL473662	178770	10	None	2	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46228133	198371	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595639	198371	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	29136870	68679	7	None	-46	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	301	2	0	7	0.7	CN1CCN(c2nc(N3CCCC3)c3cnn(C)c3n2)CC1	10.1021/jm2011589
	CHEMBL1923522	68679	7	None	-46	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	301	2	0	7	0.7	CN1CCN(c2nc(N3CCCC3)c3cnn(C)c3n2)CC1	10.1021/jm2011589
	71519176	86056	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	2	5	2.5	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314759	86056	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	2	5	2.5	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	155545717	172897	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	238	3	2	2	2.6	c1ccc2[nH]c(C[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	CHEMBL4529871	172897	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	238	3	2	2	2.6	c1ccc2[nH]c(C[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	156015791	177669	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4646286	177669	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650609	177669	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	24854638	154656	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL402470	154656	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCc2ccccc2)CC1	10.1021/jm7014217
	24854702	95611	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2cccnc2)CC1	10.1021/jm7014217
	CHEMBL259955	95611	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2cccnc2)CC1	10.1021/jm7014217
	24853592	155616	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	376	7	0	5	3.8	CN1CCN(c2nc3ccccc3nc2OCCCCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL406380	155616	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	376	7	0	5	3.8	CN1CCN(c2nc3ccccc3nc2OCCCCc2ccccc2)CC1	10.1021/jm7014217
	122193104	123370	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3627724	123370	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	137632569	155731	0	None	4	2	Human	8.5	pKi	=	8.5	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	418	7	1	2	4.8	Ic1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4065099	155731	0	None	4	2	Human	8.5	pKi	=	8.5	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	418	7	1	2	4.8	Ic1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	53233804	61197	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770966	61197	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	54583873	61200	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770969	61200	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54584824	61208	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770977	61208	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	54585780	61229	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770998	61229	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	58227326	116626	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393542	116626	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	9839217	72174	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199062	72174	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	54583873	61200	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL1770969	61200	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	54585781	61232	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	CHEMBL1771000	61232	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	71551636	89243	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	263	4	1	5	1.4	CC(C)CCc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375772	89243	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	263	4	1	5	1.4	CC(C)CCc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	25129527	186831	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm301886t
	CHEMBL493720	186831	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm301886t
	46881855	7664	0	None	15	4	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089040	7664	0	None	15	4	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	10967174	19242	3	None	2	2	Rat	8.5	pKi	=	8.5	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19242	3	None	2	2	Rat	8.5	pKi	=	8.5	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	46890166	7235	0	None	4786	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7235	0	None	4786	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1278	2119	79	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	71603136	89285	0	None	46	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	3	2	5	2.3	CNC1CN(c2nc(N)nc3cc(C4CCCC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376790	89285	0	None	46	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	3	2	5	2.3	CNC1CN(c2nc(N)nc3cc(C4CCCC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25109291	190792	9	None	-79	8	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	CHEMBL519240	190792	9	None	-79	8	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	127050309	140318	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	283	2	2	6	1.8	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1039/C6MD00108D
	CHEMBL3819470	140318	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	283	2	2	6	1.8	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1039/C6MD00108D
	53476465	67712	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	2	2	6	2.0	NCC1CN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914547	67712	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	2	2	6	2.0	NCC1CN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737947	67785	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	283	2	2	6	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914776	67785	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	283	2	2	6	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1016/j.bmcl.2011.08.014
	25071004	192116	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192116	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	25071004	192116	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192116	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	11957573	1979	29	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	1250	1979	29	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	3692	1979	29	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL19439	1979	29	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	24826358	67754	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	1	2	6	2.4	Nc1nc(N2CC3CCNC3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914745	67754	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	1	2	6	2.4	Nc1nc(N2CC3CCNC3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25101945	192504	0	None	3	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192504	0	None	3	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	58227401	175877	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175877	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175877	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	25068753	187854	15	None	1	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187854	15	None	1	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1278	2119	79	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1267	3744	43	None	-33	25	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3744	43	None	-33	25	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3744	43	None	-33	25	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	21638785	188073	4	None	4	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188073	4	None	4	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	24994636	109648	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	CHEMBL3236565	109648	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	24826441	67763	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1cccc2oc3c(N4CCN(C)CC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914754	67763	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1cccc2oc3c(N4CCN(C)CC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	10244243	140782	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL383384	140782	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	73353369	89283	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	6	0.4	CN1CCN(c2nc(N)nc3cccnc23)CC1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376788	89283	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	6	0.4	CN1CCN(c2nc(N)nc3cccnc23)CC1	10.1016/j.bmcl.2013.02.091
	11846636	77598	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209628	77598	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	155513612	175746	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	175746	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	175746	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	175746	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	206876	18756	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL128637	18756	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	206876	18756	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128637	18756	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	11249610	157424	0	None	-6309	2	Human	4.5	pKi	=	4.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	354	9	2	5	3.6	Nc1ccc(NCCc2ccc(OCCCN3CCCCC3)cc2)nc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4084820	157424	0	None	-6309	2	Human	4.5	pKi	=	4.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	354	9	2	5	3.6	Nc1ccc(NCCc2ccc(OCCCN3CCCCC3)cc2)nc1	10.1016/j.ejmech.2016.09.074
	137634777	155435	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	453	8	3	6	1.0	CCOC(=O)CN1Cc2ccccc2C[C@@H](NC(=O)C(Cc2ccccc2)C(=O)NO)C1=O	10.1016/j.ejmech.2016.09.074
	CHEMBL4061504	155435	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	453	8	3	6	1.0	CCOC(=O)CN1Cc2ccccc2C[C@@H](NC(=O)C(Cc2ccccc2)C(=O)NO)C1=O	10.1016/j.ejmech.2016.09.074
	164611474	184870	0	None	-478	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	6	4	5	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccs1	10.1016/j.ejmech.2021.113190
	CHEMBL4861704	184870	0	None	-478	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	6	4	5	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccs1	10.1016/j.ejmech.2021.113190
	164619427	185487	0	None	-7	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	5	4	4	3.1	N/C(=N\C(=O)NCCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4871221	185487	0	None	-7	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	5	4	4	3.1	N/C(=N\C(=O)NCCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	164627774	185983	0	None	-58	6	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	344	3	4	4	1.4	N/C(=N\C(=O)NCc1ccccc1)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	CHEMBL4878215	185983	0	None	-58	6	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	344	3	4	4	1.4	N/C(=N\C(=O)NCc1ccccc1)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	24994634	2111	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2111	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2111	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2111	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2111	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2111	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	155552341	175810	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543928	175810	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595906	175810	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	44129283	199342	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3ccc(Cl)c(Cl)c3n2)CC1	10.1021/jm901379s
	CHEMBL602330	199342	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3ccc(Cl)c(Cl)c3n2)CC1	10.1021/jm901379s
	11846299	138690	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	CHEMBL378788	138690	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	10934567	19062	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	CHEMBL129026	19062	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	10934567	19062	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL129026	19062	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	25128833	187818	30	None	-4	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL501882	187818	30	None	-4	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1016/j.bmcl.2011.10.104
	24826354	67798	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914790	67798	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	10934567	19062	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL129026	19062	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	16006947	138528	0	None	1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL378619	138528	0	None	1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	136030655	56188	0	None	-3	3	Human	7.5	pKi	=	7.5	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56188	0	None	-3	3	Human	7.5	pKi	=	7.5	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	9923162	18751	5	None	199	2	Human	7.5	pKi	=	7.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL128613	18751	5	None	199	2	Human	7.5	pKi	=	7.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	170389	205253	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634086	205253	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL91353	205253	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1274	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	3063228	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	CHEMBL1196470	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	170389	205253	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL4634086	205253	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL91353	205253	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681808	89218	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	3	8	3.1	CC(C)CNc1nccc(NCCN(C)c2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375748	89218	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	3	8	3.1	CC(C)CNc1nccc(NCCN(C)c2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	1274	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	3063228	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	CHEMBL1196470	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	56851608	71286	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	CHEMBL1923030	71286	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	CHEMBL1963461	71286	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	1274	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	3063228	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	CHEMBL1196470	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	1278	2119	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2119	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2119	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2119	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	66572105	75285	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047263	75285	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572106	75286	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3c(Cl)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047264	75286	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3c(Cl)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573463	75319	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	2	3.1	Cc1cc(Cl)cc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047454	75319	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	2	3.1	Cc1cc(Cl)cc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70696622	75334	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047469	75334	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10988959	117605	4	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL340740	117605	4	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10087533	194336	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202322	194336	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560068	194336	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	46929648	16580	0	None	-6	3	Human	6.5	pKi	=	6.5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	CHEMBL1243395	16580	0	None	-6	3	Human	6.5	pKi	=	6.5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	137650793	156900	0	None	-1096	2	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	484	7	1	4	4.5	O=C(NCCCCN1CCN(c2cccc(Cl)c2Cl)CC1)c1cncc(Br)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4078910	156900	0	None	-1096	2	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	484	7	1	4	4.5	O=C(NCCCCN1CCN(c2cccc(Cl)c2Cl)CC1)c1cncc(Br)c1	10.1016/j.ejmech.2016.09.074
	46912442	10803	0	None	-575	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	271	4	1	3	2.9	COc1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1172775	10803	0	None	-575	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	271	4	1	3	2.9	COc1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	57392460	69523	0	None	14	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938843	69523	0	None	14	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.bmcl.2011.11.098
	136192886	56186	0	None	-6	4	Rat	6.5	pKi	=	6.5	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56186	0	None	-6	4	Rat	6.5	pKi	=	6.5	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1248	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	3077	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	90045	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	CHEMBL12344	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	1239	2002	23	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1240	2002	23	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	912	2002	23	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL268490	2002	23	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	71681976	89221	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	5	4	5	2.0	Nc1nccc(NCCNC(=O)c2cc3cc(Cl)ccc3[nH]2)n1	10.1021/jm301886t
	CHEMBL2375751	89221	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	5	4	5	2.0	Nc1nccc(NCCNC(=O)c2cc3cc(Cl)ccc3[nH]2)n1	10.1021/jm301886t
	10023327	168450	0	None	-144	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168450	0	None	-144	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11622672	74669	2	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	1	2	2.6	c1nc(CCCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203389	74669	2	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	1	2	2.6	c1nc(CCCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	11679919	75190	0	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204413	75190	0	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11701199	75221	0	None	-3	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	1	2	2.1	CCN(CC)CCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204615	75221	0	None	-3	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	1	2	2.1	CCN(CC)CCCCc1c[nH]cn1	10.1021/jm0504353
	1248	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	3077	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	90045	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	CHEMBL12344	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	4060730	19183	51	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19183	51	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	10377814	75290	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3c(N)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047268	75290	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3c(N)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1248	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	3077	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	90045	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	CHEMBL12344	1396	27	None	-15	6	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	4563101	66425	11	None	1	2	Human	5.5	pKi	=	5.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	265	4	2	2	2.1	CN(C)CCNC(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL185842	66425	11	None	1	2	Human	5.5	pKi	=	5.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	265	4	2	2	2.1	CN(C)CCNC(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	71681977	89222	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	344	5	3	5	2.4	CN(CCNc1ccnc(N)n1)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1021/jm301886t
	CHEMBL2375752	89222	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	344	5	3	5	2.4	CN(CCNc1ccnc(N)n1)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1021/jm301886t
	71681479	89230	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	393	9	5	9	2.7	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375760	89230	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	393	9	5	9	2.7	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	3063453	12663	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187950	12663	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL534498	12663	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	112936341	136949	1	None	-12	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2nc(NCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753849	136949	1	None	-12	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2nc(NCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	164628701	185885	0	None	-186	3	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	5	4	4	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4876743	185885	0	None	-186	3	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	5	4	4	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC1CCCCC1	10.1016/j.ejmech.2021.113190
	57402928	69522	0	None	9	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	0	4	1.4	CN1CCN(C(=O)c2cn3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938842	69522	0	None	9	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	0	4	1.4	CN1CCN(C(=O)c2cn3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.11.098
	121238071	166687	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	271	2	1	7	0.3	CN1CCN(c2nc(N)nc(-c3ccncc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4292486	166687	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	271	2	1	7	0.3	CN1CCN(c2nc(N)nc(-c3ccncc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	11120826	18730	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128505	18730	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	11120826	18730	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128505	18730	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0341047
	49841720	69380	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3c(-c4ccccc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935557	69380	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3c(-c4ccccc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	44275465	98764	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL281590	98764	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	46890545	7304	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086460	7304	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11120826	18730	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128505	18730	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	57399829	67988	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	235	7	2	5	1.3	CN(C)CCNc1cc(NCC2CC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916505	67988	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	235	7	2	5	1.3	CN(C)CCNc1cc(NCC2CC2)ncn1	10.1016/j.bmcl.2011.07.114
	44126357	115415	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(N)nc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354813	115415	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(N)nc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	57393772	67835	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Br)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915041	67835	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Br)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	24854637	95872	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc3ccccc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261363	95872	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc3ccccc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	11738520	138736	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	CHEMBL1794919	138736	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	CHEMBL379044	138736	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	60150162	173294	0	None	-524	5	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	479	16	2	4	4.8	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccccc1	10.1039/C8MD00527C
	CHEMBL4539444	173294	0	None	-524	5	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	479	16	2	4	4.8	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccccc1	10.1039/C8MD00527C
	164624107	185140	0	None	-70	6	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	9	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4865765	185140	0	None	-70	6	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	9	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCc1ccccc1	10.1016/j.ejmech.2021.113190
	164608941	183801	0	None	-75	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4845733	183801	0	None	-75	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	57392890	67974	0	None	-1	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	370	7	1	5	2.5	COc1cccc(C(O)(COc2ccccc2C)CN2CCN(C)CC2)c1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916492	67974	0	None	-1	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	370	7	1	5	2.5	COc1cccc(C(O)(COc2ccccc2C)CN2CCN(C)CC2)c1	10.1016/j.bmcl.2011.07.114
	21029584	188279	4	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncnc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL506969	188279	4	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncnc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	1267	3744	43	None	-33	25	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	3035905	3744	43	None	-33	25	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	CHEMBL260374	3744	43	None	-33	25	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	90655029	109646	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.4	Nc1nc(C2CCCC2)cc(N2C[C@@H]3CNC[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236563	109646	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.4	Nc1nc(C2CCCC2)cc(N2C[C@@H]3CNC[C@@H]3C2)n1	10.1021/jm401727m
	71518862	86071	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	342	2	2	5	2.6	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314775	86071	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	342	2	2	5	2.6	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	44547567	116617	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393533	116617	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	24826163	67824	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915020	67824	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	57393773	67837	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	1	7	1.8	CN1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915044	67837	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	1	7	1.8	CN1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	11572877	66745	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL187285	66745	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	44129210	197804	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	7	2	7	1.4	CNS(=O)(=O)CCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591711	197804	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	7	2	7	1.4	CNS(=O)(=O)CCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	156015638	177697	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177697	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177697	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25179127	188491	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL509852	188491	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25179127	188491	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL509852	188491	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	71518863	86072	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	338	3	2	6	2.0	COc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314776	86072	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	338	3	2	6	2.0	COc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	8070960	155151	10	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	258	2	0	5	1.4	COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL405044	155151	10	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	258	2	0	5	1.4	COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	25130576	187043	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130576	187043	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187043	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187043	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	86277887	123294	22	None	-	1	Human	5.5	pKi	=	5.5	Binding	GPCRScan assay: inhibition of H4GPCRScan assay: inhibition of H4	ChEMBL	802	18	0	11	7.4	COc1ccc(CC[C@@H](OC(=O)[C@@H]2CCCCN2C(=O)[C@H](c2cc(OC)c(OC)c(OC)c2)C2CCCCC2)c2cccc(OCCN3CCOCC3)c2)cc1OC	10.6019/CHEMBL5058648
	CHEMBL3623630	123294	22	None	-	1	Human	5.5	pKi	=	5.5	Binding	GPCRScan assay: inhibition of H4GPCRScan assay: inhibition of H4	ChEMBL	802	18	0	11	7.4	COc1ccc(CC[C@@H](OC(=O)[C@@H]2CCCCN2C(=O)[C@H](c2cc(OC)c(OC)c(OC)c2)C2CCCCC2)c2cccc(OCCN3CCOCC3)c2)cc1OC	10.6019/CHEMBL5058648
	168294445	192408	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208113	192408	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222768	192408	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	25130235	187004	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	187004	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187004	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	187004	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	46228076	200415	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	354	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(C(C)(C)C)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL608864	200415	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	354	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(C(C)(C)C)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46228158	200472	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL609289	200472	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	57402514	67833	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Br)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915039	67833	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Br)cc3c2n1	10.1016/j.bmcl.2011.08.014
	164619039	185566	0	None	-17	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	4	4	4	3.0	N/C(=N\C(=O)NCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4872277	185566	0	None	-17	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	4	4	4	3.0	N/C(=N\C(=O)NCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	57392889	67973	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	379	4	1	5	2.2	CN1CCN(c2cc(C(=O)NCc3cccc(C(F)(F)F)c3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916491	67973	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	379	4	1	5	2.2	CN1CCN(c2cc(C(=O)NCc3cccc(C(F)(F)F)c3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	25130235	187004	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	187004	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	57393774	67838	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.4	Nc1nc(N2CCNCC2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915045	67838	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.4	Nc1nc(N2CCNCC2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	10083188	140403	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL382276	140403	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	1278	2119	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	1279	2119	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	4908365	2119	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	CHEMBL129198	2119	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	71603137	89286	0	None	23	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	1	2	5	1.9	CC(C)(C)c1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376791	89286	0	None	23	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	1	2	5	1.9	CC(C)(C)c1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25179125	188437	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL509142	188437	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179125	188437	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL509142	188437	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	127051047	139781	0	None	2	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806162	139781	0	None	2	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	7015108	198224	8	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	3	0	7	1.7	CN1CCN(c2ncnc3c2nnn3Cc2ccc(Cl)cc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594729	198224	8	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	3	0	7	1.7	CN1CCN(c2ncnc3c2nnn3Cc2ccc(Cl)cc2)CC1	10.1016/j.bmc.2009.08.059
	46228070	198043	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	271	4	0	5	1.1	CN1CCN(Cc2cn(Cc3ccccc3)nn2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593448	198043	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	271	4	0	5	1.1	CN1CCN(Cc2cn(Cc3ccccc3)nn2)CC1	10.1016/j.bmc.2009.08.059
	122193100	123401	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	314	3	1	6	1.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc(F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628047	123401	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	314	3	1	6	1.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc(F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	155513612	175746	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	127050018	140237	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	221	2	2	5	0.5	CN[C@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1039/C6MD00108D
	CHEMBL3818402	140237	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	221	2	2	5	0.5	CN[C@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1039/C6MD00108D
	24994950	109664	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.5	CN[C@@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1021/jm401727m
	CHEMBL3236580	109664	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.5	CN[C@@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1021/jm401727m
	54583875	61226	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770995	61226	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	90655028	109640	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236557	109640	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@H](N)C2)n1	10.1021/jm401727m
	59777262	109655	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236572	109655	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	155512725	175667	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175667	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175667	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	46929553	16574	0	None	-5	2	Human	6.4	pKi	=	6.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2cccnc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243366	16574	0	None	-5	2	Human	6.4	pKi	=	6.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2cccnc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	71519011	86037	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	376	2	2	5	3.3	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)c(Cl)c4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314496	86037	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	376	2	2	5	3.3	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)c(Cl)c4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	24826598	67827	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	2	7	1.9	COc1ccc2oc3c(N4CCC[C@H](N)C4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915027	67827	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	2	7	1.9	COc1ccc2oc3c(N4CCC[C@H](N)C4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	25178939	186267	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm800876b
	CHEMBL489464	186267	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm800876b
	24853529	95677	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	336	3	0	5	3.5	CN1CCN(c2nc3ccccc3nc2Sc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260388	95677	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	336	3	0	5	3.5	CN1CCN(c2nc3ccccc3nc2Sc2ccccc2)CC1	10.1021/jm7014217
	25178939	186267	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm901379s
	CHEMBL489464	186267	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm901379s
	164611757	184320	0	None	-50	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	2.8	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4853107	184320	0	None	-50	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	2.8	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	112936357	136834	1	None	-18	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752978	136834	1	None	-18	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	71519346	86061	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	2	6	-0.0	Cn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314764	86061	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	2	6	-0.0	Cn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	58227144	116639	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2cnnc(NC3CC4CCC3C4)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393555	116639	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2cnnc(NC3CC4CCC3C4)c2)CC1	10.1016/j.bmcl.2014.12.027
	57399018	67840	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	332	1	1	7	2.1	CN1CCCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915047	67840	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	332	1	1	7	2.1	CN1CCCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	16082831	79936	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL214311	79936	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	25178554	172855	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm800876b
	CHEMBL452870	172855	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm800876b
	25178554	172855	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm901379s
	CHEMBL452870	172855	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm901379s
	164610892	184615	0	None	-52	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	8	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CC1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4857560	184615	0	None	-52	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	8	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CC1CCCCC1	10.1016/j.ejmech.2021.113190
	71518864	86073	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	Cc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314777	86073	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	Cc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	122194626	123504	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629829	123504	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2015.08.014
	57399019	67844	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	2	2	7	1.4	NCC1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915051	67844	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	2	2	7	1.4	NCC1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	90645146	111685	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	288	2	1	6	1.0	CN1CCN(c2nc(N)nc(-c3ccc(F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290577	111685	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	288	2	1	6	1.0	CN1CCN(c2nc(N)nc(-c3ccc(F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	54584374	62794	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774590	62794	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789360	62794	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	122193105	123405	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3ccc4ccccc4c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628051	123405	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3ccc4ccccc4c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	921599	19255	44	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0341047
	CHEMBL129660	19255	44	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0341047
	921599	19255	44	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0502081
	CHEMBL129660	19255	44	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0502081
	4060730	19183	51	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL129199	19183	51	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.02.097
	25178553	172854	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm800876b
	CHEMBL452869	172854	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm800876b
	25178553	172854	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm901379s
	CHEMBL452869	172854	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm901379s
	11846465	141400	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	CHEMBL387054	141400	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	56851606	71256	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1923029	71256	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1963244	71256	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	51354877	58601	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCc2cn(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688988	58601	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCc2cn(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	137658819	158763	0	None	-7585	2	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	467	9	1	5	3.9	COc1cc2c(cc1OC)C(C)=C(C(=O)NCCCCN1CCN(c3ccccc3F)CC1)C2	10.1016/j.ejmech.2016.09.074
	CHEMBL4099779	158763	0	None	-7585	2	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	467	9	1	5	3.9	COc1cc2c(cc1OC)C(C)=C(C(=O)NCCCCN1CCN(c3ccccc3F)CC1)C2	10.1016/j.ejmech.2016.09.074
	54584374	62794	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774590	62794	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789360	62794	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	58227207	116635	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393551	116635	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	51354872	59883	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1688983	59883	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1739923	59883	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	68081433	109634	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	248	1	1	3	0.7	CN1CCN(C(=O)c2nc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	CHEMBL3236551	109634	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	248	1	1	3	0.7	CN1CCN(C(=O)c2nc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	57399747	67795	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	2	2	6	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914787	67795	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	2	2	6	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)C1	10.1016/j.bmcl.2011.08.014
	25129169	186598	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186598	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	25129169	186598	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL492231	186598	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	3032915	15321	20	None	-1	6	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL12160	15321	20	None	-1	6	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681806	89215	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	331	8	4	9	1.0	CC(C)CNc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375667	89215	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	331	8	4	9	1.0	CC(C)CNc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681639	89232	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	321	6	3	7	2.3	Cc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375762	89232	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	321	6	3	7	2.3	Cc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	11492393	3997	4	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	1263	3997	4	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	CHEMBL206346	3997	4	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	11557610	74098	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	7	2	2	2.7	c1nc(CCCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL202744	74098	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	7	2	2	2.7	c1nc(CCCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	11615406	138962	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	2	2	2.3	c1nc(CCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL379447	138962	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	2	2	2.3	c1nc(CCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	66572116	75308	1	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	2	2	1.5	Cc1cccc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047443	75308	1	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	2	2	1.5	Cc1cccc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70681961	75320	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.6	CN1CCCN(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047455	75320	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.6	CN1CCCN(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70696621	75327	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	2	2.2	O=C(c1cc2cccc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047462	75327	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	2	2.2	O=C(c1cc2cccc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	70692531	75340	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.0	Cc1ccc(F)c2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047475	75340	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.0	Cc1ccc(F)c2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24759623	95902	16	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm800876b
	CHEMBL261551	95902	16	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm800876b
	25129169	186598	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186598	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	24759623	95902	16	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261551	95902	16	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	10429016	194486	0	None	-5	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	366	7	3	3	3.6	N=C(NCCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL561250	194486	0	None	-5	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	366	7	3	3	3.6	N=C(NCCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1223	940	32	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	71519012	86038	0	None	218	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.8	Nc1nc(N2CCNCC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314497	86038	0	None	218	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.8	Nc1nc(N2CCNCC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	10934158	108090	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL320331	108090	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	71681478	89229	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	4	8	3.2	CC(C)CCc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375759	89229	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	4	8	3.2	CC(C)CCc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	11629829	75669	0	None	-19	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	5	1	2	2.2	c1nc(CCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL205583	75669	0	None	-19	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	5	1	2	2.2	c1nc(CCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	25831	12898	26	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	CHEMBL1189742	12898	26	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	CHEMBL539343	12898	26	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	1278	2119	79	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2119	79	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2119	79	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2119	79	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	66573456	75310	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	2	2	2	1.5	CNC1CN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047445	75310	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	2	2	2	1.5	CNC1CN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	43398194	75321	21	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	CN1CCCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047456	75321	21	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	CN1CCCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70694582	75325	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047460	75325	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70686223	75330	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	2	2	2	1.4	CNC1CN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047465	75330	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	2	2	2	1.4	CNC1CN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	70692530	75339	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047474	75339	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1238	2051	11	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	6603865	2051	11	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL11919	2051	11	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	44417445	12693	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1188154	12693	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL535387	12693	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	66572102	75282	1	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ncccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047260	75282	1	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ncccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	24854634	155615	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	400	4	0	4	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2CCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL406379	155615	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	400	4	0	4	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2CCc2ccccc2)CC1	10.1021/jm7014217
	24853528	166529	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	329	6	0	6	1.7	CN(C)CCCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL428964	166529	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	329	6	0	6	1.7	CN(C)CCCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	137659490	158650	0	None	11	2	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	420	7	1	2	6.1	Brc1ccc(C(OCCCc2c[nH]cn2)c2ccc3ccccc3c2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4098585	158650	0	None	11	2	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	420	7	1	2	6.1	Brc1ccc(C(OCCCc2c[nH]cn2)c2ccc3ccccc3c2)cc1	10.1016/j.ejmech.2016.09.074
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	24803709	145397	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cc(F)cc2[nH]1	nan
	CHEMBL3917992	145397	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cc(F)cc2[nH]1	nan
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	49841846	69395	0	None	18	3	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69395	0	None	18	3	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	44129130	197875	0	None	-2	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL592379	197875	0	None	-2	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.10.104
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	24745337	61210	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770979	61210	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227340	116616	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393532	116616	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	44129133	197704	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	398	6	2	7	1.0	CNS(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591011	197704	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	398	6	2	7	1.0	CNS(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	1223	940	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	2790	940	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	CHEMBL14690	940	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	1223	940	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	2790	940	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	CHEMBL14690	940	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	25129169	186598	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL492231	186598	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	44129134	197827	0	None	22	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	197827	0	None	22	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25129169	186598	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186598	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	1278	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	1279	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	4908365	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	24745506	67877	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915537	67877	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	136042266	69568	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938975	69568	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	136042268	69570	0	None	186	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938977	69570	0	None	186	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	25068753	187854	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187854	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1278	2119	79	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1279	2119	79	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	4908365	2119	79	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	CHEMBL129198	2119	79	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	25068753	187854	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187854	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	2063135	8293	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1093180	8293	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	24826437	67755	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@H]3CCCN[C@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914746	67755	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@H]3CCCN[C@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25128823	192115	0	None	2	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192115	0	None	2	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	1204	1901	114	None	-3	23	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1901	114	None	-3	23	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1901	114	None	-3	23	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1901	114	None	-3	23	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1901	114	None	-3	23	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1901	114	None	-3	23	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1278	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	1279	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	4908365	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	CHEMBL129198	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	44129130	197875	0	None	-2	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	197875	0	None	-2	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	54581941	61228	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	288	2	2	6	0.8	Nc1nc(N2CCCC2)cc(N2CC3CCCNC3C2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770997	61228	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	288	2	2	6	0.8	Nc1nc(N2CCCC2)cc(N2CC3CCCNC3C2)n1	10.1016/j.bmcl.2011.03.017
	24826680	67786	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914777	67786	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	11601630	132296	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL370135	132296	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	137640214	156425	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	394	6	1	2	5.5	O=C(NCCCCN1Cc2cccc3cccc(c23)C1)c1ccc2ccccc2c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4072893	156425	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	394	6	1	2	5.5	O=C(NCCCCN1Cc2cccc3cccc(c23)C1)c1ccc2ccccc2c1	10.1016/j.ejmech.2016.09.074
	71519013	86039	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	3	2	5	2.5	CCN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314498	86039	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	3	2	5	2.5	CCN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	168284289	192304	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5194860	192304	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222123	192304	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	3076002	178702	2	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL473099	178702	2	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	3076002	178702	2	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL473099	178702	2	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	90645150	111681	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290573	111681	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	25130576	187043	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130576	187043	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187043	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187043	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	10018335	71668	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL197477	71668	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	57393771	67832	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	311	2	2	7	1.5	COc1ccc2oc3c(N4C[C@@H]5C[C@H]4CN5)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915035	67832	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	311	2	2	7	1.5	COc1ccc2oc3c(N4C[C@@H]5C[C@H]4CN5)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	10545988	62817	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774593	62817	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789558	62817	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	46228071	198494	15	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596562	198494	15	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmc.2009.08.059
	1204	1901	114	None	-15	23	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1901	114	None	-15	23	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1901	114	None	-15	23	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1901	114	None	-15	23	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1901	114	None	-15	23	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1901	114	None	-15	23	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	46890590	6946	0	None	1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6946	0	None	1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57400735	67826	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	2	6	2.9	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915026	67826	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	2	6	2.9	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	56851441	71255	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923023	71255	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963243	71255	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	25147772	172837	4	None	-1	3	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL452847	172837	4	None	-1	3	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2015.12.035
	16061961	197997	0	None	7	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	197997	0	None	7	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10381810	203463	0	None	-2	5	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL7828	203463	0	None	-2	5	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	137647794	157438	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	464	8	1	6	4.2	COc1ccc2c(c1)c(CC(=O)NCCCn1ccnc1)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4085023	157438	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	464	8	1	6	4.2	COc1ccc2c(c1)c(CC(=O)NCCCn1ccnc1)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	10545988	62817	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774593	62817	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789558	62817	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	122193096	123397	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628043	123397	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645154	111689	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	1.9	CN1CCN(c2nc(N)nc(-c3ccc(C(F)(F)F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290581	111689	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	1.9	CN1CCN(c2nc(N)nc(-c3ccc(C(F)(F)F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10777275	5818	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079553	5818	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL156005	5818	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	1267	3744	43	None	-33	25	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	3035905	3744	43	None	-33	25	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	CHEMBL260374	3744	43	None	-33	25	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	127036978	136757	0	None	1	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752225	136757	0	None	1	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	164612037	184795	0	None	-81283	20	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	326	8	4	4	1.8	CCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4860528	184795	0	None	-81283	20	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	326	8	4	4	1.8	CCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	1224	1404	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	3100	1404	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	8980	1404	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	916	1404	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	CHEMBL657	1404	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	DB01075	1404	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	9883231	112887	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.0	CN1CCN(C(=O)c2nc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL331773	112887	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.0	CN1CCN(C(=O)c2nc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	1267	3744	43	None	-33	25	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	3035905	3744	43	None	-33	25	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	CHEMBL260374	3744	43	None	-33	25	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	1267	3744	43	None	-33	25	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	3035905	3744	43	None	-33	25	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	CHEMBL260374	3744	43	None	-33	25	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	24992735	109666	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	CHEMBL3236582	109666	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	24826081	67771	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914762	67771	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	90645153	111688	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290580	111688	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2014.06.032
	25178344	186303	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489664	186303	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10934158	108090	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL320331	108090	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	24853593	95612	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccncc2)CC1	10.1021/jm7014217
	CHEMBL259956	95612	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccncc2)CC1	10.1021/jm7014217
	54586765	61213	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(NCCO)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770982	61213	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(NCCO)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	44275465	98764	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2013.02.091
	CHEMBL281590	98764	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2013.02.091
	24826080	67773	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	1	1	6	3.2	CN1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914764	67773	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	1	1	6	3.2	CN1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	71519349	86064	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	6	1.8	Cn1c(-c2ccc(F)cc2)cc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314767	86064	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	6	1.8	Cn1c(-c2ccc(F)cc2)cc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	57393770	67829	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915031	67829	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	155534519	175909	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4470527	175909	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596640	175909	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	51354873	58598	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688984	58598	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	51354874	58599	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	335	6	1	4	3.5	Clc1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1Cl	10.1021/jm1013488
	CHEMBL1688985	58599	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	335	6	1	4	3.5	Clc1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1Cl	10.1021/jm1013488
	168292954	192395	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5202592	192395	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222660	192395	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	11779175	62836	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774600	62836	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789801	62836	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164612760	184135	0	None	-38	4	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4850490	184135	0	None	-38	4	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	11779175	62836	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774600	62836	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789801	62836	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	58227085	116629	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393545	116629	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	1267	3744	43	None	-33	25	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	3035905	3744	43	None	-33	25	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	CHEMBL260374	3744	43	None	-33	25	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	11846464	78051	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	CHEMBL211170	78051	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	118720433	115410	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	206	3	1	4	0.8	CCc1cc(N2CC[C@@H](NC)C2)ncn1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354807	115410	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	206	3	1	4	0.8	CCc1cc(N2CC[C@@H](NC)C2)ncn1	10.1016/j.bmcl.2014.10.013
	51354581	58589	0	None	-4	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	275	10	1	4	3.1	CCCCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688973	58589	0	None	-4	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	275	10	1	4	3.1	CCCCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	9877454	203901	1	None	-4	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL81644	203901	1	None	-4	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	1613	2316	44	None	-112	44	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	205	2316	44	None	-112	44	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	3964	2316	44	None	-112	44	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	CHEMBL831	2316	44	None	-112	44	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	DB00408	2316	44	None	-112	44	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	164609493	183854	0	None	-2630	6	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	350	6	4	5	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccco1	10.1016/j.ejmech.2021.113190
	CHEMBL4846380	183854	0	None	-2630	6	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	350	6	4	5	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccco1	10.1016/j.ejmech.2021.113190
	71518698	86066	2	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	218	1	3	5	-0.1	Nc1nc(N2CCNCC2)c2cc[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314770	86066	2	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	218	1	3	5	-0.1	Nc1nc(N2CCNCC2)c2cc[nH]c2n1	10.1016/j.bmcl.2012.10.139
	4242975	18654	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	CHEMBL128088	18654	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	4242975	18654	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL128088	18654	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	16061961	197997	0	None	7	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	197997	0	None	7	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	11258458	83040	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	CHEMBL219930	83040	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	57398183	67710	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	347	4	3	7	1.8	Nc1nc(N2CC[C@@H](NCCO)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914545	67710	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	347	4	3	7	1.8	Nc1nc(N2CC[C@@H](NCCO)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	4242975	18654	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128088	18654	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	118720438	115416	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	220	3	1	4	1.4	CN[C@@H]1CCN(c2ccnc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354814	115416	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	220	3	1	4	1.4	CN[C@@H]1CCN(c2ccnc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	24854640	95613	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccccn2)CC1	10.1021/jm7014217
	CHEMBL259957	95613	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccccn2)CC1	10.1021/jm7014217
	135589846	141288	3	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	356	2	2	5	3.1	OCCN1CCN(C2=Nc3cc(Cl)ccc3Nc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL386333	141288	3	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	356	2	2	5	3.1	OCCN1CCN(C2=Nc3cc(Cl)ccc3Nc3ccccc32)CC1	10.1021/jm051008s
	24826439	67780	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	2	2	7	1.4	COc1cccc2oc3c(N4CCNCC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914771	67780	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	2	2	7	1.4	COc1cccc2oc3c(N4CCNCC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	10446295	2101	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	1277	2101	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL185951	2101	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	10446295	2101	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	1277	2101	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL185951	2101	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	10023327	168450	0	None	-144	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168450	0	None	-144	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	1267	3744	43	None	-33	25	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	3035905	3744	43	None	-33	25	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	CHEMBL260374	3744	43	None	-33	25	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	155556721	173982	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	248	2	1	6	0.6	CC(C)=Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4556243	173982	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	248	2	1	6	0.6	CC(C)=Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	24854639	95689	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	5	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260458	95689	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	5	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCCc2ccccc2)CC1	10.1021/jm7014217
	11344458	203487	2	None	-14	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78497	203487	2	None	-14	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	51354588	59855	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	CHEMBL1688980	59855	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	CHEMBL1739759	59855	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	16106	117643	28	None	-20	11	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL340801	117643	28	None	-20	11	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	10.1021/jm051008s
	46881855	7664	0	None	-15	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089040	7664	0	None	-15	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	71519014	86040	0	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	2	5	2.9	CC(C)N1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314499	86040	0	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	2	5	2.9	CC(C)N1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	122193094	123395	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628041	123395	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	155513612	175746	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175746	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175746	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175746	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	45379808	199534	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	362	5	2	6	1.6	CC(=O)NCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL603621	199534	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	362	5	2	6	1.6	CC(=O)NCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	168286426	192324	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5198795	192324	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222241	192324	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	164620139	185206	0	None	-56	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	436	8	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4866734	185206	0	None	-56	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	436	8	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	136042267	69569	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938976	69569	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11623296	116522	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338972	116522	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	11066922	19225	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129436	19225	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	11066922	19225	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129436	19225	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0341047
	49841845	69396	0	None	19	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69396	0	None	19	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	25068753	187854	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.10.104
	CHEMBL502347	187854	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.10.104
	1223	940	32	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	2790	940	32	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	CHEMBL14690	940	32	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	1278	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	1278	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	1279	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	1279	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	4908365	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	4908365	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	CHEMBL129198	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	CHEMBL129198	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	58227086	116641	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393557	116641	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	1279	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	4908365	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	CHEMBL129198	2119	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	2063135	8293	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1021/jm401727m
	CHEMBL1093180	8293	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1021/jm401727m
	23650959	109632	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	410	7	1	4	5.3	Cc1cnc(OCCCCC2CCN(C)CC2)cc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/jm401727m
	CHEMBL3236548	109632	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	410	7	1	4	5.3	Cc1cnc(OCCCCC2CCN(C)CC2)cc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/jm401727m
	11066922	19225	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129436	19225	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	155524115	175973	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	175973	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	175973	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	70696620	75315	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	1	2	3	1.8	CN1CCN(C(=O)c2cc3cc(Cl)cc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047450	75315	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	1	2	3	1.8	CN1CCN(C(=O)c2cc3cc(Cl)cc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	25129523	186704	40	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	186704	40	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	76311195	105571	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	284	2	2	6	1.1	CN1CCN(c2cc(-c3ccc(N)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL3133130	105571	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	284	2	2	6	1.1	CN1CCN(c2cc(-c3ccc(N)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25129523	186704	40	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	186704	40	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25128824	192575	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192575	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	155524115	175973	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175973	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175973	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	25128824	192575	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192575	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	25071004	192116	0	None	-1	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192116	0	None	-1	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	11705395	15876	0	None	-1	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	CHEMBL1223749	15876	0	None	-1	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	24826162	67800	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914792	67800	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	71519674	89299	0	None	1	5	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89299	0	None	1	5	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	46890072	6832	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084355	6832	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	24994634	2111	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2111	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2111	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2111	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2111	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2111	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	44129056	197919	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	474	7	1	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592663	197919	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	474	7	1	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25128822	186898	0	None	10	5	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	186898	0	None	10	5	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	11554490	15877	0	None	-5	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.3	FC(F)(F)c1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL1223750	15877	0	None	-5	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.3	FC(F)(F)c1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1	10.1016/j.ejmech.2016.09.074
	24797956	109641	3	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	109641	3	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	24825514	186272	0	None	5	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	256	4	1	2	3.6	CC(C)(C)CC(=O)c1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	CHEMBL489493	186272	0	None	5	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	256	4	1	2	3.6	CC(C)(C)CC(=O)c1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	24825515	186734	0	None	-1	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	238	2	1	1	3.4	CC(C)(C)C#Cc1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	CHEMBL493014	186734	0	None	-1	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	238	2	1	1	3.4	CC(C)(C)C#Cc1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	11301441	95707	6	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm800876b
	CHEMBL260549	95707	6	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm800876b
	11301441	95707	6	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL260549	95707	6	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm7014217
	24825791	67713	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914548	67713	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25109291	190792	9	None	-501	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	CHEMBL519240	190792	9	None	-501	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	45379922	197882	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	552	7	2	7	2.8	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(I)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592434	197882	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	552	7	2	7	2.8	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(I)ccc3n2)CC1	10.1021/jm901379s
	54585777	61205	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	5	3	5	2.1	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770974	61205	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	5	3	5	2.1	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)c2)C1	10.1016/j.bmcl.2011.03.017
	118720441	115420	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	3	4	2.5	Nc1cc(C2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354819	115420	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	3	4	2.5	Nc1cc(C2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	24826518	67762	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2cccc(C(F)(F)F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914753	67762	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2cccc(C(F)(F)F)c23)CC1	10.1016/j.bmcl.2011.08.014
	1278	2119	79	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2119	79	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2119	79	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2119	79	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	136192886	56186	0	None	1	4	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56186	0	None	1	4	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136141537	56187	1	None	-2	3	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56187	1	None	-2	3	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	71525678	89295	0	None	-5	6	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89295	0	None	-5	6	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	1267	3744	43	None	-14	25	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	3035905	3744	43	None	-14	25	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	CHEMBL260374	3744	43	None	-14	25	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	1223	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1269	121	33	None	213	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	213	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	213	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	1255	3146	11	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	6421522	3146	11	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL19173	3146	11	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	10017000	202780	0	None	1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72431	202780	0	None	1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1223	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	940	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	71681644	89237	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	412	9	4	8	3.8	CC(C)CNc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375767	89237	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	412	9	4	8	3.8	CC(C)CNc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	71681805	89242	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	426	9	3	8	3.8	CC(C)CNc1nccc(NCCN(C)c2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375771	89242	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	426	9	3	8	3.8	CC(C)CNc1nccc(NCCN(C)c2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	11506928	74093	0	None	-1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	2	2	3.0	c1nc(CCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL202728	74093	0	None	-1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	2	2	3.0	c1nc(CCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	11600779	75695	0	None	-6	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205731	75695	0	None	-6	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	11536119	138178	0	None	-15	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	249	7	2	2	3.4	c1nc(CCCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL377954	138178	0	None	-15	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	249	7	2	2	3.4	c1nc(CCCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	4242975	18654	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL128088	18654	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	45379921	197524	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	426	6	1	7	3.6	CN1CCN(c2nc(NCCc3ccc([N+](=O)[O-])cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL589800	197524	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	426	6	1	7	3.6	CN1CCN(c2nc(NCCc3ccc([N+](=O)[O-])cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	9972181	193547	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202331	193547	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL550757	193547	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10063835	62793	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774601	62793	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789356	62793	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	46890200	7081	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	442	9	1	4	5.4	CN(C)CCN(C)CCCOc1ccc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)cc1Cl	10.1016/j.bmcl.2010.04.017
	CHEMBL1085403	7081	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	442	9	1	4	5.4	CN(C)CCN(C)CCCOc1ccc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)cc1Cl	10.1016/j.bmcl.2010.04.017
	1267	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-15	23	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-15	23	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-15	23	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-15	23	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-15	23	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-15	23	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1267	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	71681803	89186	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)ncn1	10.1021/jm301886t
	CHEMBL2375363	89186	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)ncn1	10.1021/jm301886t
	11672774	3998	6	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	1245	3998	6	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	CHEMBL206548	3998	6	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	11564628	75180	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	193	5	1	2	1.8	c1nc(CCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL204360	75180	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	193	5	1	2	1.8	c1nc(CCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	11629931	75958	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	5	1	2	2.6	c1nc(CCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL205868	75958	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	5	1	2	2.6	c1nc(CCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	70686222	75329	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	2	2	2	1.7	CN[C@@H]1CCN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047464	75329	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	2	2	2	1.7	CN[C@@H]1CCN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	135398737	944	89	None	-57	91	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	38	944	89	None	-57	91	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	722	944	89	None	-57	91	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	CHEMBL42	944	89	None	-57	91	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	DB00363	944	89	None	-57	91	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	44395314	123169	0	None	-3	2	Human	5.3	pKi	=	5.3	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	2	2	2	3.2	CN1CCC(C(O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL362163	123169	0	None	-3	2	Human	5.3	pKi	=	5.3	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	2	2	2	3.2	CN1CCC(C(O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	126688	400	28	None	1	3	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	4025	400	28	None	1	3	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	CHEMBL293762	400	28	None	1	3	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	66572110	75291	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047269	75291	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	70684085	75322	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	325	1	1	2	3.0	CN1CCCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047457	75322	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	325	1	1	2	3.0	CN1CCCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10063835	62793	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774601	62793	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789356	62793	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	206876	18756	7	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1039/C2MD20212C
	CHEMBL128637	18756	7	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1039/C2MD20212C
	57395620	67874	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915534	67874	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	57395620	67874	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915534	67874	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	90645160	111695	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3c(F)cccc3F)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290587	111695	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3c(F)cccc3F)n2)CC1	10.1016/j.ejmech.2014.06.032
	57390145	69563	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938970	69563	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	49865668	15875	0	None	-51	4	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	390	8	1	5	4.3	COc1ccccc1N1CCN(CCCCNc2nccc3ccccc23)CC1	10.1016/j.ejmech.2016.09.074
	CHEMBL1223748	15875	0	None	-51	4	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	390	8	1	5	4.3	COc1ccccc1N1CCN(CCCCNc2nccc3ccccc23)CC1	10.1016/j.ejmech.2016.09.074
	54586764	61209	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N(C)C(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770978	61209	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N(C)C(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	44546966	116609	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	4	1	4	1.9	CC(C)CNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393525	116609	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	4	1	4	1.9	CC(C)CNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	9964223	203974	11	None	-36	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL82244	203974	11	None	-36	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	90645148	111683	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290575	111683	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	56950378	69525	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938845	69525	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	25071940	186771	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	186771	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	90645145	111686	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL3290578	111686	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	66741667	109649	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236566	109649	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	122194623	123501	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	296	3	1	6	1.4	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629826	123501	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	296	3	1	6	1.4	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645145	111686	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290578	111686	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10083861	15332	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)nc2c1	10.1021/jm0502081
	CHEMBL121679	15332	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)nc2c1	10.1021/jm0502081
	121238067	166558	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4290148	166558	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	57394636	67704	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	284	1	0	5	2.6	CN1CCN(c2ncnc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914534	67704	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	284	1	0	5	2.6	CN1CCN(c2ncnc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	71525587	89297	0	None	177	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	255	2	2	7	-0.1	CNC1CN(c2nc(N)nc3cc(C#N)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376802	89297	0	None	177	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	255	2	2	7	-0.1	CNC1CN(c2nc(N)nc3cc(C#N)cnc23)C1	10.1016/j.bmcl.2013.02.091
	753256	178770	10	None	2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL473662	178770	10	None	2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmcl.2012.10.139
	46228143	198400	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595880	198400	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	24745335	292	7	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	24745335	292	7	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	51354583	58591	0	None	2	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.1	c1nc(CCCn2cc(CC3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688975	58591	0	None	2	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.1	c1nc(CCCn2cc(CC3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	71518860	86069	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	2	2	5	2.0	CN1CCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314773	86069	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	2	2	5	2.0	CN1CCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	46228113	200388	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	328	5	2	7	1.4	COc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL608717	200388	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	328	5	2	7	1.4	COc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	164611807	184401	0	None	-162	4	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4854278	184401	0	None	-162	4	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	49841718	69392	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)cc1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935569	69392	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)cc1	10.1016/j.bmcl.2011.10.104
	58227143	116625	1	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393541	116625	1	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	67087450	116634	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	116634	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	46890071	6831	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.7	Cc1cc(OCCCNCC2CCCN(C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084354	6831	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.7	Cc1cc(OCCCNCC2CCCN(C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740470	109310	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3233048	109310	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	2306318	86580	12	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL232421	86580	12	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	121238068	156668	0	None	-281	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	1	6	2.0	CN1CCN(c2nc(N)nc(Cc3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4076046	156668	0	None	-281	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	1	6	2.0	CN1CCN(c2nc(N)nc(Cc3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178341	186227	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489271	186227	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178341	186227	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489271	186227	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25178556	188496	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL509961	188496	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	23649429	95850	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	2	0	5	2.7	COc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL261224	95850	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	2	0	5	2.7	COc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	44478385	5699	2	None	9	4	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078642	5699	2	None	9	4	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	25178344	186303	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489664	186303	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	24853597	95849	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1cccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)c1	10.1021/jm7014217
	CHEMBL261223	95849	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1cccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)c1	10.1021/jm7014217
	8070044	155411	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	300	4	0	5	2.4	CC(C)COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL406123	155411	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	300	4	0	5	2.4	CC(C)COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	16061844	61212	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(NCc3ccccc3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770981	61212	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(NCc3ccccc3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	57403351	67981	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2cc(NCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916499	67981	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2cc(NCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	156011443	177766	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4636487	177766	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651225	177766	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25178339	186201	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489061	186201	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354585	58593	0	None	-5	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	245	5	1	4	2.0	c1nc(CCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688977	58593	0	None	-5	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	245	5	1	4	2.0	c1nc(CCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	51354876	59884	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1739924	59884	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL3216267	59884	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	12741697	95713	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL260565	95713	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	25178339	186201	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489061	186201	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	919019	136209	7	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2[nH]c3c(N4CCN(C)CC4)ncnc3c2c1	10.1039/C5MD00022J
	CHEMBL3740837	136209	7	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2[nH]c3c(N4CCN(C)CC4)ncnc3c2c1	10.1039/C5MD00022J
	929854	197407	8	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	289	4	2	4	2.7	CN(C)CCNc1ncnc2c1[nH]c1ccc(Cl)cc12	10.1039/C5MD00022J
	CHEMBL588176	197407	8	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	289	4	2	4	2.7	CN(C)CCNc1ncnc2c1[nH]c1ccc(Cl)cc12	10.1039/C5MD00022J
	168278543	192249	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184846	192249	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221764	192249	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168295803	192443	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207206	192443	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222937	192443	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	57402471	67534	0	None	-549	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	8	1	5	4.0	COc1ccc(CN(CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910378	67534	0	None	-549	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	8	1	5	4.0	COc1ccc(CN(CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	164614645	184549	0	None	-57	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	CHEMBL4856572	184549	0	None	-57	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	57394627	67979	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	5	2	5	2.4	CN1CC[C@H](Nc2cc(NCCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916497	67979	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	5	2	5	2.4	CN1CC[C@H](Nc2cc(NCCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	71519179	86059	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	3	4	5	2.1	Nc1nc(N[C@H]2CCNC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314762	86059	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	3	4	5	2.1	Nc1nc(N[C@H]2CCNC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	1204	1901	114	None	1	23	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1901	114	None	1	23	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1901	114	None	1	23	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1901	114	None	1	23	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1901	114	None	1	23	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1901	114	None	1	23	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1278	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1278	2119	79	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2119	79	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2119	79	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2119	79	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	44538833	69376	21	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@H]2CCCC[C@H]2O1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935450	69376	21	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@H]2CCCC[C@H]2O1	10.1016/j.bmcl.2011.10.104
	49841719	69394	0	None	38	3	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69394	0	None	38	3	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	11846300	79856	3	None	-2	4	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL213912	79856	3	None	-2	4	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2011.10.104
	11301441	95707	6	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260549	95707	6	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1016/j.bmcl.2011.10.104
	54582865	61190	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770959	61190	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	54584825	61221	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770990	61221	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25130912	188153	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL505061	188153	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	54584825	61221	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL1770990	61221	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	58227157	115417	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354815	115417	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	58227200	116619	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393535	116619	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	58227337	116637	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393553	116637	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	24745335	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	11957573	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1250	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3692	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19439	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	44595856	89227	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375757	89227	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	1278	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	1279	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	4908365	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	CHEMBL129198	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	11957573	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	1250	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	3692	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL19439	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	25129523	186704	40	None	1	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL492884	186704	40	None	1	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.06.123
	1278	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2119	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	11066922	19225	1	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129436	19225	1	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573458	75312	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047447	75312	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573459	75313	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047448	75313	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	9860842	75314	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)cc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047449	75314	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)cc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	11957573	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	1250	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	3692	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL19439	1979	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	71525681	89298	0	None	2	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89298	0	None	2	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	136042270	69572	0	None	426	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938979	69572	0	None	426	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1204	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	1247	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	1375	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	774	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL90	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	DB05381	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	25178552	172839	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452848	172839	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178552	172839	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL452848	172839	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	90645158	111693	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(Cl)cc1-c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290585	111693	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(Cl)cc1-c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2014.06.032
	57399828	67985	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	345	5	1	5	3.8	CC(C)(C)CCNc1cc(N2CCN(C3CCCCC3)CC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916502	67985	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	345	5	1	5	3.8	CC(C)(C)CCNc1cc(N2CCN(C3CCCCC3)CC2)ncn1	10.1016/j.bmcl.2011.07.114
	805994	173783	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL455133	173783	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm800876b
	805994	173783	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL455133	173783	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm901379s
	168286141	192322	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5192993	192322	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222231	192322	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	46890167	7178	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN1CCC(N(C)CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085872	7178	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN1CCC(N(C)CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	9966927	18687	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128252	18687	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9966927	18687	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128252	18687	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0341047
	9966927	18687	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128252	18687	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	12686479	161246	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1021/jm7014217
	CHEMBL414183	161246	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1021/jm7014217
	1265	929	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	6422124	929	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	CHEMBL14638	929	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	1265	929	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	6422124	929	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	CHEMBL14638	929	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	57390262	67843	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	290	1	2	7	1.2	Nc1nc(N2CC(N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915050	67843	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	290	1	2	7	1.2	Nc1nc(N2CC(N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	155552102	175989	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4576393	175989	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597352	175989	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	25178936	186200	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489060	186200	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178936	186200	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489060	186200	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	137646103	157364	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	393	8	3	3	3.7	O=C(O)C(Cc1cccc2ccccc12)NC(=O)[C@@H](CS)Cc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4084275	157364	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	393	8	3	3	3.7	O=C(O)C(Cc1cccc2ccccc12)NC(=O)[C@@H](CS)Cc1ccccc1	10.1016/j.ejmech.2016.09.074
	42624149	109651	1	None	-3	3	Mouse	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	109651	1	None	-3	3	Mouse	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	58227127	116627	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393543	116627	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	1204	1901	114	None	-12	23	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-12	23	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-12	23	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-12	23	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-12	23	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-12	23	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	44395308	66494	0	None	39	2	Human	7.2	pKi	=	7.2	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	369	1	1	2	2.2	CN1CCN(C(=O)c2cc3cc(I)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186108	66494	0	None	39	2	Human	7.2	pKi	=	7.2	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	369	1	1	2	2.2	CN1CCN(C(=O)c2cc3cc(I)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	155552061	175904	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175904	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175904	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	1204	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10017821	103310	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL308913	103310	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1901	114	None	-3	23	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10023327	168450	0	None	-144	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168450	0	None	-144	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11499454	74071	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	6	2	2	2.5	CC(C)(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL202638	74071	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	6	2	2	2.5	CC(C)(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	10446240	74649	1	None	-50	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	277	8	2	2	3.6	Clc1ccc(CNCCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	CHEMBL203263	74649	1	None	-50	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	277	8	2	2	3.6	Clc1ccc(CNCCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	11622495	76224	0	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206303	76224	0	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	1267	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	3035905	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	CHEMBL260374	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	12686479	161246	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL414183	161246	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1016/j.bmcl.2011.06.123
	25129523	186704	40	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	186704	40	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25129527	186831	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	186831	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	70681962	75332	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.3	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047467	75332	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.3	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCNCC1	10.1016/j.ejmech.2012.06.016
	70688339	75333	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047468	75333	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1267	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	3035905	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	CHEMBL260374	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	1267	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	1267	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	3035905	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	3035905	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	CHEMBL260374	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	CHEMBL260374	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	24854635	95895	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	310	1	0	4	3.0	Cc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL261519	95895	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	310	1	0	4	3.0	Cc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	10085528	193524	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202330	193524	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL550570	193524	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10041484	193580	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	316	9	3	3	3.6	N=C(NCCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL551047	193580	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	316	9	3	3	3.6	N=C(NCCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1267	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-33	25	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1252	1986	16	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	9793868	1986	16	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL417096	1986	16	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	71681641	89234	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	350	8	4	8	2.2	CC(C)CCc1cc(NCCNc2cc(Cl)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375764	89234	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	350	8	4	8	2.2	CC(C)CCc1cc(NCCNc2cc(Cl)nc(N)n2)nc(N)n1	10.1021/jm301886t
	11492189	75653	0	None	-10	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	1	2	2.5	CCN(CC)CCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205511	75653	0	None	-10	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	1	2	2.5	CCN(CC)CCCCCc1c[nH]cn1	10.1021/jm0504353
	14712103	61556	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	201	5	2	2	1.7	c1ccc(CNCCc2c[nH]cn2)cc1	10.1016/j.bmcl.2011.06.123
	CHEMBL17740	61556	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	201	5	2	2	1.7	c1ccc(CNCCc2c[nH]cn2)cc1	10.1016/j.bmcl.2011.06.123
	12741697	95713	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL260565	95713	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.06.123
	9793921	64797	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	164	2	1	1	2.5	c1nc(CC2CCCCC2)c[nH]1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824228	64797	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	164	2	1	1	2.5	c1nc(CC2CCCCC2)c[nH]1	10.1016/j.bmcl.2011.06.123
	24764570	75309	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	2	1.9	CN[C@@H]1CCN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047444	75309	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	2	1.9	CN[C@@H]1CCN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	25178938	186177	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm901379s
	CHEMBL488885	186177	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm901379s
	933006	136050	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2c(c1)[nH]c1c(N3CCN(C)CC3)ncnc12	10.1039/C5MD00022J
	CHEMBL3739430	136050	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2c(c1)[nH]c1c(N3CCN(C)CC3)ncnc12	10.1039/C5MD00022J
	10419556	5286	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106913	5286	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	168273549	192201	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174342	192201	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221438	192201	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	66572103	75283	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cnccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047261	75283	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cnccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	164627020	185826	0	None	-144	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	422	7	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4876025	185826	0	None	-144	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	422	7	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	10515993	5884	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079918	5884	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL154944	5884	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	54587792	61225	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.2	Nc1nc(N2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770994	61225	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.2	Nc1nc(N2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1016/j.bmcl.2011.03.017
	90645156	111691	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	313	3	1	7	0.9	CN1CCN(c2nc(N)nc(-c3ccc(N(C)C)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290583	111691	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	313	3	1	7	0.9	CN1CCN(c2nc(N)nc(-c3ccc(N(C)C)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	763018	136211	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	6	1.3	Cc1nnc2c(N3CCN(C)CC3)nc3ccccc3n12	10.1039/C5MD00022J
	CHEMBL3740846	136211	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	6	1.3	Cc1nnc2c(N3CCN(C)CC3)nc3ccccc3n12	10.1039/C5MD00022J
	44414498	79586	0	None	-173	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	7	0	2	6.9	CN(Cc1ccc(-c2ccc(CN(C)C3CCCCC3)cc2)cc1)C1CCCCC1	10.1016/j.bmc.2008.10.029
	CHEMBL212775	79586	0	None	-173	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	7	0	2	6.9	CN(Cc1ccc(-c2ccc(CN(C)C3CCCCC3)cc2)cc1)C1CCCCC1	10.1016/j.bmc.2008.10.029
	44414433	138999	0	None	-8	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	352	13	2	2	5.9	CCCCCNCc1ccc(-c2ccc(CNCCCCC)cc2)cc1	10.1016/j.bmc.2008.10.029
	CHEMBL379545	138999	0	None	-8	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	352	13	2	2	5.9	CCCCCNCc1ccc(-c2ccc(CNCCCCC)cc2)cc1	10.1016/j.bmc.2008.10.029
	10419556	5286	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106913	5286	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	164623155	184993	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	352	3	4	4	3.4	N/C(=N\C(=O)Nc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4863393	184993	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	352	3	4	4	3.4	N/C(=N\C(=O)Nc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	57395260	69386	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2nc(N)nc(N3CCN(C)CC3)c2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935563	69386	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2nc(N)nc(N3CCN(C)CC3)c2c1	10.1016/j.bmcl.2011.10.104
	11408069	83848	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	CHEMBL221211	83848	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	11408069	83848	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL221211	83848	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	24825995	67803	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914795	67803	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	1267	3744	43	None	-21	25	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-21	25	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-21	25	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	762810	198225	11	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	262	2	0	5	2.0	CCc1cc2c(N3CCN(C)CC3)ncnc2s1	10.1016/j.bmc.2009.08.059
	CHEMBL594730	198225	11	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	262	2	0	5	2.0	CCc1cc2c(N3CCN(C)CC3)ncnc2s1	10.1016/j.bmc.2009.08.059
	51354299	58580	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	247	8	1	4	2.4	CCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688945	58580	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	247	8	1	4	2.4	CCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	136773074	156823	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4077914	156823	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	57400473	69384	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935561	69384	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)c1	10.1016/j.bmcl.2011.10.104
	57397301	67538	0	None	-891	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	560	11	1	6	4.6	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910382	67538	0	None	-891	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	560	11	1	6	4.6	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	127026018	136967	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753985	136967	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	73346048	91435	0	None	-15	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	7	2	3	3.0	O=C(NCCc1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL2413359	91435	0	None	-15	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	7	2	3	3.0	O=C(NCCc1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164617783	184007	0	None	-104	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	8	4	4	2.8	CCC(CNC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848815	184007	0	None	-104	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	8	4	4	2.8	CCC(CNC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	71519499	86065	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	1	6	2.1	CN1CCN(c2nc(N)nc3c2cc(-c2ccc(F)cc2)n3C)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314768	86065	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	1	6	2.1	CN1CCN(c2nc(N)nc3c2cc(-c2ccc(F)cc2)n3C)CC1	10.1016/j.bmcl.2012.10.139
	54583872	61191	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770960	61191	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)n2)C1	10.1016/j.bmcl.2011.03.017
	1278	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	1279	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	4908365	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	CHEMBL129198	2119	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	58227388	116622	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393538	116622	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	155517880	176093	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176093	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176093	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	155517880	176093	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176093	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176093	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	46890626	6510	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@@H]3CCCN[C@@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083097	6510	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@@H]3CCCN[C@@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	25178938	186177	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm800876b
	CHEMBL488885	186177	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm800876b
	9812843	203533	1	None	-4	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78838	203533	1	None	-4	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	647957	28864	12	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	2	0	6	0.4	Cc1cc(N2CCN(C)CC2)nc(N2CCOCC2)n1	10.1039/C5MD00022J
	CHEMBL1381010	28864	12	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	2	0	6	0.4	Cc1cc(N2CCN(C)CC2)nc(N2CCOCC2)n1	10.1039/C5MD00022J
	747187	136115	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	249	4	1	3	2.8	Cc1cc2nc(SCCN(C)C)[nH]c2cc1C	10.1039/C5MD00022J
	CHEMBL3739949	136115	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	249	4	1	3	2.8	Cc1cc2nc(SCCN(C)C)[nH]c2cc1C	10.1039/C5MD00022J
	168268716	192160	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5170276	192160	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221176	192160	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168268853	192183	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5180504	192183	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221353	192183	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	5013	99773	28	None	-16	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2ccc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL288591	99773	28	None	-16	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2ccc3ccccc3n2)CC1	10.1021/jm7014217
	10243990	5817	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079552	5817	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL155830	5817	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	11065080	19236	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1007/s00044-004-0106-7
	CHEMBL129488	19236	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1007/s00044-004-0106-7
	11065080	19236	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0341047
	CHEMBL129488	19236	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0341047
	117724523	116613	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393529	116613	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	11594278	14521	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL120591	14521	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	11065080	19236	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL129488	19236	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	44462956	5523	0	None	-2	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL1077276	5523	0	None	-2	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	71603140	89289	0	None	97	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	3	2	5	1.5	CNC1CN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376794	89289	0	None	97	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	3	2	5	1.5	CNC1CN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25147772	172837	4	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	172837	4	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25147772	172837	4	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL452847	172837	4	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	44129131	197773	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	440	8	1	7	2.1	CCN(CC)S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591500	197773	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	440	8	1	7	2.1	CCN(CC)S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	25071319	188474	8	None	9	2	Rat	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188474	8	None	9	2	Rat	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	16082828	79850	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213886	79850	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	57398091	67766	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	3.0	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914757	67766	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	3.0	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	57401517	67783	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)c(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914774	67783	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)c(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	3035746	187597	6	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL1237146	187597	6	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL498770	187597	6	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	136030655	56188	0	None	2	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56188	0	None	2	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136192874	56189	0	None	1	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56189	0	None	1	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1204	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1247	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1375	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	774	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1204	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	53321919	56184	0	None	-3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632409	56184	0	None	-3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10060213	102438	1	None	3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL306077	102438	1	None	3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	155491015	175921	3	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	175921	3	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	175921	3	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	44609412	89244	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	322	6	4	8	1.6	Nc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375773	89244	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	322	6	4	8	1.6	Nc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	25129523	186704	40	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm301886t
	CHEMBL492884	186704	40	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm301886t
	51354584	58592	0	None	11	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	6	1	4	2.4	c1nc(CCCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688976	58592	0	None	11	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	6	1	4	2.4	c1nc(CCCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	76314798	105572	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	314	3	1	7	1.4	CN1CCN(c2cc(-c3ccc([N+](=O)[O-])cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL3133131	105572	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	314	3	1	7	1.4	CN1CCN(c2cc(-c3ccc([N+](=O)[O-])cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	136042269	69571	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938978	69571	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	71525678	89295	0	None	-7	6	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89295	0	None	-7	6	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25178937	186202	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL489062	186202	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25071319	188474	8	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188474	8	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	44129209	200344	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	586	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccc(I)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL608373	200344	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	586	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccc(I)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11013044	18722	1	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	333	4	1	2	3.2	O=C(c1cc2ccccc2[nH]1)N1CCN(CCc2ccccc2)CC1	10.1021/jm0341047
	CHEMBL128470	18722	1	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	333	4	1	2	3.2	O=C(c1cc2ccccc2[nH]1)N1CCN(CCc2ccccc2)CC1	10.1021/jm0341047
	122194625	123503	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629828	123503	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	52952957	62833	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774594	62833	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789799	62833	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	24854703	166334	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	364	5	0	6	3.0	COc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL428606	166334	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	364	5	0	6	3.0	COc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	156013005	176925	0	None	-2	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	899	20	2	7	10.6	Cc1cc(OCCCNC(=O)CCCCCN2/C(=C\C=C\C=C\C3=[N+](CCCCS(=O)(=O)[O-])c4ccccc4C3(C)C)C(C)(C)c3ccccc32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4639472	176925	0	None	-2	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	899	20	2	7	10.6	Cc1cc(OCCCNC(=O)CCCCCN2/C(=C\C=C\C=C\C3=[N+](CCCCS(=O)(=O)[O-])c4ccccc4C3(C)C)C(C)(C)c3ccccc32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	168268827	192177	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5179906	192177	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221314	192177	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	52952957	62833	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774594	62833	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789799	62833	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	25071319	188474	8	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188474	8	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	46228101	198116	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594022	198116	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46228083	198059	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	374	5	2	6	3.1	CN1CCN(c2cc(NCc3ccc(-c4ccccc4)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593551	198059	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	374	5	2	6	3.1	CN1CCN(c2cc(NCc3ccc(-c4ccccc4)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890549	6507	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083085	6507	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	112936352	136969	1	None	-17	2	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2cc(-c3ccccc3)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3754008	136969	1	None	-17	2	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2cc(-c3ccccc3)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	155563489	174757	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.7	CCC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4573929	174757	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.7	CCC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71518865	86035	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	333	2	2	6	1.8	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(C#N)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314494	86035	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	333	2	2	6	1.8	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(C#N)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	57401167	69526	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938846	69526	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24826679	67760	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914751	67760	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	168294161	192404	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204739	192404	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222742	192404	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	168294770	192418	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208327	192418	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222806	192418	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	44450438	95675	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc3ccccc3nc2NCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260371	95675	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc3ccccc3nc2NCc2ccccc2)CC1	10.1021/jm7014217
	71528209	86544	0	None	-1148	7	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	465	6	0	3	5.8	CC1=CC(C)=[N+]2C1=C(c1ccc(OCCCN3CCCCC3)cc1)c1c(C)cc(C)n1[B-]2(F)F	10.1021/ml300383n
	CHEMBL2323582	86544	0	None	-1148	7	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	465	6	0	3	5.8	CC1=CC(C)=[N+]2C1=C(c1ccc(OCCCN3CCCCC3)cc1)c1c(C)cc(C)n1[B-]2(F)F	10.1021/ml300383n
	121238069	158580	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting methodDisplacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.04.033
	CHEMBL4097856	158580	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting methodDisplacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.04.033
	24826268	67709	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	3	2	6	2.8	CCN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914544	67709	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	3	2	6	2.8	CCN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	56851443	71284	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923024	71284	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963459	71284	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	121238069	158580	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4097856	158580	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178132	186356	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL490073	186356	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25178132	186356	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL490073	186356	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	168283321	192293	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183015	192293	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222057	192293	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	24759622	169112	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL443764	169112	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm800876b
	24759622	169112	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm7014217
	24759622	169112	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL443764	169112	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL443764	169112	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm901379s
	164622259	185148	0	None	-7244	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	336	6	4	5	1.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccco1	10.1016/j.ejmech.2021.113190
	CHEMBL4865965	185148	0	None	-7244	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	336	6	4	5	1.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccco1	10.1016/j.ejmech.2021.113190
	24826167	67804	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914796	67804	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	54581938	61203	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770972	61203	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	46890019	7231	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7231	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	24994945	109650	1	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CN[C@@H]1CCN(c2cc(C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236567	109650	1	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CN[C@@H]1CCN(c2cc(C)nc(N)n2)C1	10.1021/jm401727m
	1223	940	32	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	940	32	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	940	32	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	51354301	58584	0	None	-10	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688969	58584	0	None	-10	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	164609017	183843	0	None	-13	6	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	10	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4846201	183843	0	None	-13	6	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	10	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCCc1ccccc1	10.1016/j.ejmech.2021.113190
	168294166	192412	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209081	192412	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222775	192412	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	168294178	192433	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5205197	192433	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222887	192433	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	127026019	136935	0	None	-6	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	378	2	0	6	1.7	CN1CCN(c2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753705	136935	0	None	-6	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	378	2	0	6	1.7	CN1CCN(c2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	11546127	95040	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL25725	95040	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	10715823	5853	0	None	-20	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	281	10	2	3	3.3	CCCCC(CC)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079747	5853	0	None	-20	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	281	10	2	3	3.3	CCCCC(CC)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	1267	3744	43	None	-33	25	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3744	43	None	-33	25	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3744	43	None	-33	25	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	25071319	188474	8	None	-9	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.06.123
	CHEMBL509540	188474	8	None	-9	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.06.123
	25109291	190792	9	None	-501	8	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1016/j.bmcl.2011.06.123
	CHEMBL519240	190792	9	None	-501	8	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1016/j.bmcl.2011.06.123
	1204	1901	114	None	-15	23	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1901	114	None	-15	23	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1901	114	None	-15	23	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1901	114	None	-15	23	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1901	114	None	-15	23	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1901	114	None	-15	23	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10446295	2101	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	1277	2101	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL185951	2101	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	1267	3744	43	None	-21	25	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3744	43	None	-21	25	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3744	43	None	-21	25	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	937120	174529	8	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	244	1	0	3	1.8	CN1CCN(C(=O)c2cc3ccccc3o2)CC1	10.1016/j.bmc.2020.115924
	CHEMBL4568949	174529	8	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	244	1	0	3	1.8	CN1CCN(C(=O)c2cc3ccccc3o2)CC1	10.1016/j.bmc.2020.115924
	71681974	89219	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1cc(-c2ccccc2)nc(N)n1)c1ccnc(N)n1	10.1021/jm301886t
	CHEMBL2375749	89219	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1cc(-c2ccccc2)nc(N)n1)c1ccnc(N)n1	10.1021/jm301886t
	71681475	89225	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	317	8	5	9	1.0	CC(C)CNc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375755	89225	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	317	8	5	9	1.0	CC(C)CNc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681643	89236	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375766	89236	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	71681645	89238	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375768	89238	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	11679993	75664	0	None	-25	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.0	CC(C)CNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205559	75664	0	None	-25	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.0	CC(C)CNCCCCc1c[nH]cn1	10.1021/jm0504353
	11087453	75292	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047270	75292	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572113	75297	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3ccc(N)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047275	75297	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3ccc(N)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573457	75311	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	2	1	2	2.4	Cc1cccc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047446	75311	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	2	1	2	2.4	Cc1cccc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70688340	75341	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3c(F)ccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047476	75341	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3c(F)ccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	51354875	58600	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688986	58600	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25178937	186202	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL489062	186202	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	11846635	78270	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	CHEMBL211242	78270	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	4060730	19183	51	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19183	51	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	90645159	111694	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	2.2	CN1CCN(c2nc(N)nc(-c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290586	111694	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	2.2	CN1CCN(c2nc(N)nc(-c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	162671115	182365	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	266	2	0	3	1.9	CN1CCN(CC2Cc3cc(Cl)ccc3O2)CC1	10.1016/j.bmc.2020.115924
	CHEMBL4792753	182365	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	266	2	0	3	1.9	CN1CCN(CC2Cc3cc(Cl)ccc3O2)CC1	10.1016/j.bmc.2020.115924
	10197870	5229	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106612	5229	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	91613	17804	31	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL12620	17804	31	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	71681474	89224	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	246	5	4	8	-0.0	Nc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375754	89224	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	246	5	4	8	-0.0	Nc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	11637064	74605	0	None	-25	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	1	2	2.2	c1nc(CCCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203228	74605	0	None	-25	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	1	2	2.2	c1nc(CCCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	11708125	76166	3	None	-251	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	5	1	2	1.3	CN(C)CCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206000	76166	3	None	-251	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	5	1	2	1.3	CN(C)CCCCc1c[nH]cn1	10.1021/jm0504353
	11499376	137974	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	5	2	2	2.1	CC(C)(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL377491	137974	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	5	2	2	2.1	CC(C)(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	56672434	64793	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	1	2	3.3	CN1CCC(=Cc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824048	64793	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	1	2	3.3	CN1CCC(=Cc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2011.06.123
	66572109	75289	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3c([N+](=O)[O-])cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047267	75289	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3c([N+](=O)[O-])cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10957150	75306	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cccc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047441	75306	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cccc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	3939214	126124	7	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	262	1	1	1	3.4	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCCC1	10.1016/j.bmcl.2004.08.035
	CHEMBL365257	126124	7	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	262	1	1	1	3.4	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCCC1	10.1016/j.bmcl.2004.08.035
	11086114	7313	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL108649	7313	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	2247	502	77	None	-117	41	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	249	502	77	None	-117	41	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	2603	502	77	None	-117	41	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	CHEMBL296419	502	77	None	-117	41	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	DB00637	502	77	None	-117	41	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	44417471	12662	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187948	12662	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	CHEMBL534496	12662	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	164614841	184692	0	None	-223	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	7	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	CHEMBL4858849	184692	0	None	-223	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	7	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	9923162	18751	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128613	18751	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10967174	19242	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129540	19242	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	1204	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	9923162	18751	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128613	18751	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0341047
	10967174	19242	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19242	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	1278	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	54580746	61062	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.1	Nc1nc(N2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770160	61062	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.1	Nc1nc(N2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2011.03.017
	1278	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1279	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	4908365	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL129198	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	58227157	115417	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354815	115417	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	58227179	116632	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393548	116632	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	58227259	116640	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	116640	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	42624150	109660	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236577	109660	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	24827129	67793	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2c(Cl)cc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914785	67793	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2c(Cl)cc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	9923162	18751	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128613	18751	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	10967174	19242	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129540	19242	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	44129212	197772	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.3	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(C(F)(F)F)cccc3n2)CC1	10.1021/jm901379s
	CHEMBL591496	197772	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.3	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(C(F)(F)F)cccc3n2)CC1	10.1021/jm901379s
	24825522	187060	0	None	-6	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	283	3	1	3	3.3	CC1CC(C)(C)N=C(c2cccc(Cc3c[nH]cn3)c2)O1	10.1021/jm7014149
	CHEMBL494982	187060	0	None	-6	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	283	3	1	3	3.3	CC1CC(C)(C)N=C(c2cccc(Cc3c[nH]cn3)c2)O1	10.1021/jm7014149
	51354300	58581	0	None	15	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688946	58581	0	None	15	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	58227373	175934	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175934	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175934	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	168294182	192446	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208722	192446	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222960	192446	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	46890546	6437	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6437	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228069	198466	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	3	1	7	1.2	CN1CCN(c2nc(N)nc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596348	198466	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	3	1	7	1.2	CN1CCN(c2nc(N)nc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	63868172	175482	2	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	222	2	1	6	-0.2	CCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4590628	175482	2	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	222	2	1	6	-0.2	CCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	164614549	184360	0	None	-575	6	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	340	9	4	4	2.2	CCCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4853668	184360	0	None	-575	6	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	340	9	4	4	2.2	CCCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	164621681	184998	0	None	-64	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	444	7	4	5	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(OC(F)(F)F)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4863523	184998	0	None	-64	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	444	7	4	5	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(OC(F)(F)F)cc1	10.1016/j.ejmech.2021.113190
	164627241	185847	0	None	-269	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	6	4	4	3.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Br)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4876267	185847	0	None	-269	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	6	4	4	3.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Br)cc1	10.1016/j.ejmech.2021.113190
	11086114	7313	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL108649	7313	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	71519347	86062	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	274	4	2	6	1.2	CCCCn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314765	86062	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	274	4	2	6	1.2	CCCCn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	54754351	67875	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915535	67875	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	54754351	67875	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915535	67875	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	58227242	116636	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2cnnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393552	116636	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2cnnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	121238067	166558	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2017.09.035
	CHEMBL4290148	166558	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2017.09.035
	57400778	67868	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915346	67868	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	54584827	61227	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770996	61227	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	57400778	67868	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915346	67868	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400589	69565	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)cnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938972	69565	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)cnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	90645164	111699	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1C	10.1016/j.ejmech.2014.06.032
	CHEMBL3290591	111699	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1C	10.1016/j.ejmech.2014.06.032
	57399016	67834	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915040	67834	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	46890548	6506	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083084	6506	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46929551	16565	0	None	-52	2	Human	6.1	pKi	=	6.1	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2ccccn2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243335	16565	0	None	-52	2	Human	6.1	pKi	=	6.1	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2ccccn2)c2c[nH]cn2)cc1	10.1021/jm100643t
	5159263	10285	11	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	259	4	3	4	0.8	Cc1cc(C)nc(N/C(N)=N/CCc2c[nH]cn2)n1	10.1021/jm7014777
	CHEMBL1162351	10285	11	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	259	4	3	4	0.8	Cc1cc(C)nc(N/C(N)=N/CCc2c[nH]cn2)n1	10.1021/jm7014777
	71525679	89296	0	None	-6	6	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89296	0	None	-6	6	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25178130	189153	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL515870	189153	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	25178130	189153	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL515870	189153	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	164622153	184986	0	None	-181	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	6	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Cl)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4863347	184986	0	None	-181	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	6	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Cl)cc1	10.1016/j.ejmech.2021.113190
	54581939	61215	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770984	61215	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	122193095	123396	0	None	-3	2	Mouse	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assayDisplacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123396	0	None	-3	2	Mouse	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assayDisplacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	58227260	116630	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393546	116630	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	57398176	67986	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	273	2	0	5	1.4	CN1CCN(c2cc(N3[C@@H]4CCC[C@@H]3C4)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916503	67986	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	273	2	0	5	1.4	CN1CCN(c2cc(N3[C@@H]4CCC[C@@H]3C4)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	127050776	139717	0	None	6	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	139717	0	None	6	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	164627189	185739	0	None	-831	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	298	6	4	4	1.0	CCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4874758	185739	0	None	-831	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	298	6	4	4	1.0	CCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	71519015	86041	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	246	1	2	5	0.7	CN1CCCN(c2nc(N)nc3[nH]ccc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314500	86041	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	246	1	2	5	0.7	CN1CCCN(c2nc(N)nc3[nH]ccc23)CC1	10.1016/j.bmcl.2012.10.139
	57400737	67836	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Cl)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915042	67836	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Cl)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	57395494	67830	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915032	67830	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	57395499	67839	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	2	7	1.8	C[C@H]1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CCN1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915046	67839	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	2	7	1.8	C[C@H]1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CCN1	10.1016/j.bmcl.2011.08.014
	46890677	7210	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086038	7210	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	56851612	71257	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923032	71257	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963245	71257	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	56851602	71259	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923027	71259	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963260	71259	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	25178345	172874	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccco3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452925	172874	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccco3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354303	58587	0	None	-1	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.7	c1nc(CCCn2cc(C3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688971	58587	0	None	-1	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.7	c1nc(CCCn2cc(C3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25147771	173666	0	None	-1	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454890	173666	0	None	-1	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10656971	16244	4	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL123137	16244	4	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1021/jm7014217
	25179123	188511	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL510181	188511	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	10197870	5229	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106612	5229	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	168273471	192199	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173079	192199	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221433	192199	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	57402472	67537	0	None	-1819	3	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	526	11	1	6	4.0	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910381	67537	0	None	-1819	3	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	526	11	1	6	4.0	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	44576985	10284	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	212	4	7	4	-1.3	CC(/C=C(\C)NNC(=N)N)=N/NC(=N)N	10.1021/jm7014777
	CHEMBL1162347	10284	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	212	4	7	4	-1.3	CC(/C=C(\C)NNC(=N)N)=N/NC(=N)N	10.1021/jm7014777
	71519010	86036	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	386	2	2	5	2.7	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Br)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314495	86036	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	386	2	2	5	2.7	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Br)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	1204	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	1247	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	1375	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	774	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	54587790	61211	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770980	61211	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	54753437	67841	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL1915048	67841	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2014.12.027
	11750684	83047	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	CHEMBL219982	83047	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	54753437	67841	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1021/jm401727m
	CHEMBL1915048	67841	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1021/jm401727m
	24825794	67714	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914549	67714	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	71525679	89296	0	None	6	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89296	0	None	6	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25071940	186771	0	None	5	2	Rat	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	186771	0	None	5	2	Rat	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	71525586	89294	0	None	22	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	2	1	6	0.9	CCc1cnc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376799	89294	0	None	22	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	2	1	6	0.9	CCc1cnc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	44129135	197749	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	454	6	1	8	1.1	CN1CCN(c2nc(NCCS(=O)(=O)N3CCOCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591250	197749	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	454	6	1	8	1.1	CN1CCN(c2nc(NCCS(=O)(=O)N3CCOCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	53378711	109661	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236578	109661	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	54753437	67841	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915048	67841	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	135525668	67870	0	None	263	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915348	67870	0	None	263	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	49841611	69390	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935567	69390	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	1248	1396	27	None	-15	6	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	3077	1396	27	None	-15	6	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	90045	1396	27	None	-15	6	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	CHEMBL12344	1396	27	None	-15	6	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	11615753	72593	6	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3sccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL200546	72593	6	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3sccc3[nH]2)CC1	10.1021/jm0502081
	10444761	123320	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3ccsc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL362495	123320	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3ccsc3[nH]2)CC1	10.1021/jm0502081
	16082826	79903	0	None	3	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@H]2C[C@@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214156	79903	0	None	3	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@H]2C[C@@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	122193097	123398	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628044	123398	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	57390344	67879	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	248	5	3	6	0.3	CNC1CN(c2cc(N)nc(NCC3CC3)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915539	67879	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	248	5	3	6	0.3	CNC1CN(c2cc(N)nc(NCC3CC3)n2)C1	10.1016/j.bmcl.2011.07.125
	49841607	69378	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	CHEMBL1935555	69378	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	12686451	202351	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL1743969	202351	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL6992	202351	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	51354010	58576	0	None	-1	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	281	7	1	4	2.6	c1ccc(Cc2cn(CCCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688941	58576	0	None	-1	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	281	7	1	4	2.6	c1ccc(Cc2cn(CCCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	127036979	136963	0	None	1	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753962	136963	0	None	1	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	118720434	115411	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	241	3	2	5	0.6	CN[C@@H]1CCN(c2cc(CCl)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354808	115411	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	241	3	2	5	0.6	CN[C@@H]1CCN(c2cc(CCl)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	11212855	83815	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL220934	83815	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	57401602	67987	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	242	1	0	4	1.3	Cc1ccc(C#N)c(N2CC3CN(C)CC3C2)n1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916504	67987	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	242	1	0	4	1.3	Cc1ccc(C#N)c(N2CC3CN(C)CC3C2)n1	10.1016/j.bmcl.2011.07.114
	156010537	177734	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634235	177734	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651053	177734	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	90654503	109823	0	None	-123	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	483	9	1	4	4.9	O=C1NC(c2ccccc2)(c2ccccc2)C(=O)N1Cc1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	CHEMBL3238447	109823	0	None	-123	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	483	9	1	4	4.9	O=C1NC(c2ccccc2)(c2ccccc2)C(=O)N1Cc1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	57394626	67976	0	None	14	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916494	67976	0	None	14	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	49841723	69383	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)cc1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935560	69383	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)cc1	10.1016/j.bmcl.2011.10.104
	112936251	136742	1	None	1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752085	136742	1	None	1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	25178340	186203	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm800876b
	CHEMBL489069	186203	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm800876b
	25179123	188511	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL510181	188511	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25178340	186203	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm901379s
	CHEMBL489069	186203	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm901379s
	164618690	185574	0	None	-13	5	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	3.4	CCCCCCNC(=O)/N=C(\N)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4872375	185574	0	None	-13	5	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	3.4	CCCCCCNC(=O)/N=C(\N)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	44547126	116611	1	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393527	116611	1	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	118725540	116608	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	1.9	CN[C@@H]1CCN(c2ccnc(N3CCCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393524	116608	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	1.9	CN[C@@H]1CCN(c2ccnc(N3CCCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	46890676	7163	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	1	4	4.0	Cc1cc(OCCCN2CC3CN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085804	7163	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	1	4	4.0	Cc1cc(OCCCN2CC3CN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57393651	69564	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3cc4cccnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938971	69564	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3cc4cccnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24853666	155577	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	1	1	4	1.7	CN1CCCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL406327	155577	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	1	1	4	1.7	CN1CCCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	112936267	136771	1	None	-2	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2cc(-c3ccccc3)nc(NCCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752383	136771	1	None	-2	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2cc(-c3ccccc3)nc(NCCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	127051301	139670	0	None	5	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139670	0	None	5	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130914	186671	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL492677	186671	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	71519178	86058	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314761	86058	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	127052293	139708	1	None	34	4	Human	7.0	pKi	=	7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139708	1	None	34	4	Human	7.0	pKi	=	7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1530	2151	44	None	-21379	20	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	3827	2151	44	None	-21379	20	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	7206	2151	44	None	-21379	20	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	CHEMBL534	2151	44	None	-21379	20	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	DB00920	2151	44	None	-21379	20	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	124087	1362	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	7157	1362	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	814	1362	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	CHEMBL1172	1362	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	DB00967	1362	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	71548412	1878	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	9879	1878	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	CHEMBL4790724	1878	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	71548412	1878	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	9879	1878	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	CHEMBL4790724	1878	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	1278	2119	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1279	2119	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	4908365	2119	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	CHEMBL129198	2119	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1204	1901	114	None	-12	23	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1901	114	None	-12	23	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1901	114	None	-12	23	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1901	114	None	-12	23	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1901	114	None	-12	23	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1901	114	None	-12	23	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1236	2036	23	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1237	2036	23	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	156615	2036	23	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	CHEMBL268229	2036	23	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1204	1901	114	None	-15	23	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1901	114	None	-15	23	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1901	114	None	-15	23	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1901	114	None	-15	23	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1901	114	None	-15	23	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1901	114	None	-15	23	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1241	2006	46	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1242	2006	46	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	3614	2006	46	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	CHEMBL507	2006	46	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1204	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1204	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1204	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1204	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1204	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1247	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1247	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1247	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1247	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1247	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1375	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1375	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1375	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1375	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1375	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	774	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	774	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	774	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	774	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	774	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	DB05381	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	DB05381	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	DB05381	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	DB05381	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	DB05381	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1901	114	None	-3	23	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	None	214372	0	3H-Histamine	4	6	Guinea pig	10.0	pKi	=	10	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	1204	1901	114	3H-Histamine	1	23	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-Histamine	1	23	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-Histamine	1	23	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-Histamine	1	23	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-Histamine	1	23	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-Histamine	1	23	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	None	214372	0	3H-Histamine	4	6	Guinea pig	9.8	pKi	=	9.8	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	179	396	107	3H-Histamine	-4365	50	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	2159	396	107	3H-Histamine	-4365	50	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	963	396	107	3H-Histamine	-4365	50	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	CHEMBL243712	396	107	3H-Histamine	-4365	50	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	DB06288	396	107	3H-Histamine	-4365	50	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	242	467	117	3H-Histamine	-7244	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	34	467	117	3H-Histamine	-7244	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	60795	467	117	3H-Histamine	-7244	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	CHEMBL1112	467	117	3H-Histamine	-7244	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	DB01238	467	117	3H-Histamine	-7244	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	1231	924	112	3H-Histamine	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	1231	924	112	3H-PYRILAMINE	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	2756	924	112	3H-Histamine	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	2756	924	112	3H-PYRILAMINE	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	645	924	112	3H-Histamine	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	645	924	112	3H-PYRILAMINE	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	CHEMBL30	924	112	3H-Histamine	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	CHEMBL30	924	112	3H-PYRILAMINE	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	DB00501	924	112	3H-Histamine	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	DB00501	924	112	3H-PYRILAMINE	-81	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	277	1274	55	3H-Histamine	-301	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1274	55	3H-Histamine	-301	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1274	55	3H-Histamine	-301	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1274	55	3H-Histamine	-301	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1274	55	3H-Histamine	-301	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1248	1396	27	3H-PYRILAMINE	-15	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	3077	1396	27	3H-PYRILAMINE	-15	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	90045	1396	27	3H-PYRILAMINE	-15	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	CHEMBL12344	1396	27	3H-PYRILAMINE	-15	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	1224	1404	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	3100	1404	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	8980	1404	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	916	1404	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	CHEMBL657	1404	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	DB01075	1404	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	1212	1632	45	3H-Histamine	-19952	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	204	1632	45	3H-Histamine	-19952	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	3372	1632	45	3H-Histamine	-19952	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	CHEMBL726	1632	45	3H-Histamine	-19952	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	DB00623	1632	45	3H-Histamine	-19952	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	1353	1880	85	3H-Histamine	-6918	85	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	3559	1880	85	3H-Histamine	-6918	85	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	86	1880	85	3H-Histamine	-6918	85	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	CHEMBL54	1880	85	3H-Histamine	-6918	85	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	DB00502	1880	85	3H-Histamine	-6918	85	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	135398745	2869	108	3H-Histamine	-5128	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	47	2869	108	3H-Histamine	-5128	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	CHEMBL715	2869	108	3H-Histamine	-5128	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	DB00334	2869	108	3H-Histamine	-5128	65	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	119570	3110	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	2233	3110	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	953	3110	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	CHEMBL301265	3110	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	DB00413	3110	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	2337	3205	72	3H-Histamine	-1122	62	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	50	3205	72	3H-Histamine	-1122	62	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	5002	3205	72	3H-Histamine	-1122	62	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	CHEMBL716	3205	72	3H-Histamine	-1122	62	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	DB01224	3205	72	3H-Histamine	-1122	62	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	1234	3239	0	3H-Histamine	-162	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	3001055	3239	0	3H-Histamine	-162	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	CHEMBL512	3239	0	3H-Histamine	-162	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	DB00863	3239	0	3H-Histamine	-162	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	2389	3279	114	3H-Histamine	-25703	67	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	5073	3279	114	3H-Histamine	-25703	67	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	96	3279	114	3H-Histamine	-25703	67	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	CHEMBL85	3279	114	3H-Histamine	-25703	67	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	DB00734	3279	114	3H-Histamine	-25703	67	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	6917970	3635	54	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	8370	3635	54	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	CHEMBL487387	3635	54	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	213	3792	50	3H-Histamine	-549	43	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	2717	3792	50	3H-Histamine	-549	43	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	5533	3792	50	3H-Histamine	-549	43	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	CHEMBL621	3792	50	3H-Histamine	-549	43	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	DB00656	3792	50	3H-Histamine	-549	43	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	185	3943	54	3H-Histamine	-30902	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	5311271	3943	54	3H-Histamine	-30902	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	CHEMBL74355	3943	54	3H-Histamine	-30902	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	DB16351	3943	54	3H-Histamine	-30902	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	2865	4079	67	3H-Histamine	-20417	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	59	4079	67	3H-Histamine	-20417	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	60854	4079	67	3H-Histamine	-20417	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	CHEMBL708	4079	67	3H-Histamine	-20417	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	DB00246	4079	67	3H-Histamine	-20417	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	10297	26905	29	3H-Histamine	-38	43	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	C[C@H](N)[C@H](O)c1ccccc1	None
	CHEMBL136560	26905	29	3H-Histamine	-38	43	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	C[C@H](N)[C@H](O)c1ccccc1	None
	156391	46537	95	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	CHEMBL1200806	46537	95	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	CHEMBL154	46537	95	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	10624	69957	17	3H-Histamine	-102	32	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	138543650	69957	17	3H-Histamine	-102	32	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	CHEMBL194378	69957	17	3H-Histamine	-102	32	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	21830793	91403	5	3H-8-OH-DPAT	-66069	46	Bovine	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	0	8	0.6	COc1ccccc1N1CCN(CCCCn2ncc(=O)n(C)c2=O)CC1	None
	CHEMBL2413154	91403	5	3H-8-OH-DPAT	-66069	46	Bovine	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	0	8	0.6	COc1ccccc1N1CCN(CCCCn2ncc(=O)n(C)c2=O)CC1	None
	46780481	107045	18	3H-Histamine	-58884	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	9903970	107045	18	3H-Histamine	-58884	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	CHEMBL3187365	107045	18	3H-Histamine	-58884	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	CHEMBL3544974	107045	18	3H-Histamine	-58884	53	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	446220	132998	13	3H-Histamine	-1778	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	303	3	0	5	1.9	COC(=O)[C@H]1[C@@H](OC(=O)c2ccccc2)C[C@@H]2CC[C@H]1N2C	None
	CHEMBL370805	132998	13	3H-Histamine	-1778	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	303	3	0	5	1.9	COC(=O)[C@H]1[C@@H](OC(=O)c2ccccc2)C[C@@H]2CC[C@H]1N2C	None
	4495	194860	87	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	308	5	1	5	2.8	CS(=O)(=O)Nc1ccc([N+](=O)[O-])cc1Oc1ccccc1	None
	CHEMBL56367	194860	87	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	308	5	1	5	2.8	CS(=O)(=O)Nc1ccc([N+](=O)[O-])cc1Oc1ccccc1	None
	54677470	198867	110	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	CHEMBL1256873	198867	110	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	CHEMBL599	198867	110	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	162265	200587	19	3H-Histamine	-239	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	4786	200587	19	3H-Histamine	-239	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	CHEMBL61006	200587	19	3H-Histamine	-239	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	4054	203790	64	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	CHEMBL1699	203790	64	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	CHEMBL807	203790	64	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	11954259	214201	0	3H-Histamine	-33113	43	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	443	5	0	5	3.5	CN1CCN(CC1)CCC=C2C3=CC=CC=C3SC4=C2C=C(C=C4)S(=O)(=O)N(C)C	None
	None	214217	0	3H-Histamine	-1	27	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	329	2	0	4	3.6	CC1=NC=CN1CC2CCC3=C(C2=O)C4=CC=CC=C4N3C.Cl	None
	104911	214249	0	3H-Histamine	-41686	36	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	530	7	0	5	5.1	COC1=CC=CC=C1N2CCN(CC2)CCN(C3=CC=CC=N3)C(=O)C4CCCCC4.Cl.Cl.Cl	None
	None	214372	0	3H-PYRILAMINE	-7585	6	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	25137849	214425	0	3H-Histamine	-4	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	165	3	2	2	1.3	CC(C(C1=CC=CC=C1)O)NC	None
	71290	214425	0	3H-Histamine	-4	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	165	3	2	2	1.3	CC(C(C1=CC=CC=C1)O)NC	None
	None	214560	0	3H-Histamine	-1	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	240	7	4	6	-0.8	C(C(C(=O)O)N)SSCC(C(=O)O)N	None
	None	214561	0	3H-Histamine	-1	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	149	4	2	3	0.2	CSCCC(C(=O)O)N	None
	None	214562	0	3H-Histamine	-1	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	135	3	3	3	-0.3	C(CS)C(C(=O)O)N	None
	None	214563	0	3H-Histamine	-1	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	121	2	3	3	-0.7	C(C(C(=O)O)N)S	None
	None	214564	0	3H-Histamine	-1	28	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	117	0	1	3	-0.0	C1CSC(=O)C1N	None
	None	214565	0	3H-Histamine	-1	39	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	153	3	3	3	-1.4	C(C(C(=O)O)N)S(=O)O	None
	None	214566	0	3H-Histamine	-1	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	169	3	3	4	-1.7	C(C(C(=O)O)N)S(=O)(=O)O	None
	None	214574	0	3H-Histamine	-13	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	149	2	1	2	1.2	CC(C(=O)C1=CC=CC=C1)N	None
	1576	214575	0	3H-Histamine	-16	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	163	3	1	2	1.5	CC(C(=O)C1=CC=CC=C1)NC	None
	135269	214639	0	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	222	5	1	3	2.5	CCCCC(=O)OC1=CC=CC=C1C(=O)O	None
	23681059	214640	0	3H-Histamine	-1	29	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	CC(C1=CC2=C(C=C1)C=C(C=C2)OC)C(=O)O	None
	123619	214644	0	3H-Histamine	-1412	27	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	358	3	0	4	4.2	CC1=NC=C(C=C1)C2=NC=C(C=C2C3=CC=C(C=C3)S(=O)(=O)C)Cl	None
	119828	214645	0	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	370	5	1	5	3.5	CCC(=O)NS(=O)(=O)C1=CC=C(C=C1)C2=C(ON=C2C3=CC=CC=C3)C	None
	None	214648	0	3H-Histamine	-7	29	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	517	8	2	5	5.2	CC1=C(C2=C(N1C(=O)C3=CC=C(C=C3)Cl)C=CC(=C2)OC)CC(=O)NCCC4=CC=C(C=C4)NC(=O)C	None
	None	214747	0	3H-Histamine	-4570	27	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	347	6	0	3	5.0	CC(=O)N(CC1=CC=CC=C1OC)C2=CC=CC=C2OC3=CC=CC=C3	None
	135409468	2004	63	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	333	2004	63	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	CHEMBL845	2004	63	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	1204	1901	114	3H-Histamine	1	23	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-Histamine	1	23	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-Histamine	1	23	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-Histamine	1	23	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-Histamine	1	23	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-Histamine	1	23	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1225	1443	24	3H-PYRILAMINE	-371	21	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	3958	1443	24	3H-PYRILAMINE	-371	21	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	667477	1443	24	3H-PYRILAMINE	-371	21	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	CHEMBL860	1443	24	3H-PYRILAMINE	-371	21	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	DB01142	1443	24	3H-PYRILAMINE	-371	21	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	1226	1988	16	3H-Histamine	2	6	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	41376	1988	16	3H-Histamine	2	6	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	CHEMBL12608	1988	16	3H-Histamine	2	6	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	1223	940	32	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	940	32	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	940	32	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1251	1983	34	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1983	34	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1983	34	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1253	2027	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	132937	2027	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	CHEMBL19010	2027	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	None	214502	0	3H-Histamine	-13	18	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	None	214502	0	3H-N-alpha-methylhistamine	-13	18	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	1223	940	32	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	940	32	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	940	32	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1267	3744	43	3H-Histamine	-33	25	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3744	43	3H-N-alpha-methylhistamine	-33	25	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3744	43	3H-Histamine	-33	25	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3744	43	3H-N-alpha-methylhistamine	-33	25	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3744	43	3H-Histamine	-33	25	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3744	43	3H-N-alpha-methylhistamine	-33	25	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1547484	927	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	654	927	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	9072	927	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	CHEMBL43064	927	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	DB00568	927	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	45037031	214395	0	3H-Histamine	-70	4	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@H](CC1=CN=CN1)N.Br.Br	None
	1223	940	32	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	940	32	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	940	32	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1204	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1901	114	3H-N-alpha-methylhistamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-N-alpha-methylhistamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-N-alpha-methylhistamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-N-alpha-methylhistamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-N-alpha-methylhistamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-N-alpha-methylhistamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	2286	3134	48	3H-PYRILAMINE	-10	29	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	4927	3134	48	3H-PYRILAMINE	-10	29	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	7282	3134	48	3H-PYRILAMINE	-10	29	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	CHEMBL643	3134	48	3H-PYRILAMINE	-10	29	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	DB01069	3134	48	3H-PYRILAMINE	-10	29	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	1204	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-Histamine	-3	23	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	3032915	15321	20	3H-Histamine	-1	6	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15321	20	3H-Histamine	-1	6	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	1204	1901	114	3H-JNJ 7777120	-3	23	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-JNJ 7777120	-3	23	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-JNJ 7777120	-3	23	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-JNJ 7777120	-3	23	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-JNJ 7777120	-3	23	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-JNJ 7777120	-3	23	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1251	1983	34	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1251	1983	34	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1251	1983	34	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1983	34	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1983	34	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1983	34	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1983	34	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1983	34	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1983	34	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	None	214502	0	125I-iodophenpropit	-13	18	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	None	214502	0	3H-JNJ 7777120	-13	18	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	103	4089	56	3H-Histamine	-89	53	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2875	4089	56	3H-Histamine	-89	53	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	5736	4089	56	3H-Histamine	-89	53	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL285802	4089	56	3H-Histamine	-89	53	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB09225	4089	56	3H-Histamine	-89	53	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	135398737	944	89	3H-Histamine	-57	91	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	3H-Histamine	-57	91	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	3H-Histamine	-57	91	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	3H-Histamine	-57	91	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	3H-Histamine	-57	91	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	1204	1901	114	3H-Histamine	-3	23	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-Histamine	-3	23	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-Histamine	-3	23	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-Histamine	-3	23	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-Histamine	-3	23	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-Histamine	-3	23	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	11957573	1979	29	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1979	29	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1979	29	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1979	29	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	277	1274	55	3H-PYRILAMINE	-301	45	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1274	55	3H-PYRILAMINE	-301	45	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1274	55	3H-PYRILAMINE	-301	45	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1274	55	3H-PYRILAMINE	-301	45	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1274	55	3H-PYRILAMINE	-301	45	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1241	2006	46	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	1242	2006	46	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	3614	2006	46	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	CHEMBL507	2006	46	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	None	214372	0	3H-Histamine	-7585	6	Human	6.6	pKi	=	6.6	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	100	3745	52	3H-Histamine	-56	55	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	2637	3745	52	3H-Histamine	-56	55	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	5452	3745	52	3H-Histamine	-56	55	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	CHEMBL479	3745	52	3H-Histamine	-56	55	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	DB00679	3745	52	3H-Histamine	-56	55	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	1204	1901	114	125I-iodophenpropit	-3	23	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	125I-iodophenpropit	-3	23	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	125I-iodophenpropit	-3	23	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	125I-iodophenpropit	-3	23	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	125I-iodophenpropit	-3	23	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	125I-iodophenpropit	-3	23	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1226	1988	16	3H-N-alpha-methylhistamine	2	6	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	41376	1988	16	3H-N-alpha-methylhistamine	2	6	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	CHEMBL12608	1988	16	3H-N-alpha-methylhistamine	2	6	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	45037031	214395	0	3H-N-alpha-methylhistamine	-70	4	Human	6.6	pKi	=	6.6	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@H](CC1=CN=CN1)N.Br.Br	None
	1267	3744	43	3H-Histamine	-33	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3744	43	3H-Histamine	-33	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3744	43	3H-Histamine	-33	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1210	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	1213	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2725	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	33036	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	4411	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	616	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	6976	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	716121	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	90475904	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1201353	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1554789	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL505	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB01114	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB13679	905	45	3H-PYRILAMINE	-186	20	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	135398737	944	89	125I-iodophenpropit	-57	91	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	125I-iodophenpropit	-57	91	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	125I-iodophenpropit	-57	91	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	125I-iodophenpropit	-57	91	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	125I-iodophenpropit	-57	91	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	1239	2002	23	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	1240	2002	23	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	912	2002	23	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	CHEMBL268490	2002	23	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	180	397	50	3H-PYRILAMINE	-32	38	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	200	397	50	3H-PYRILAMINE	-32	38	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	2160	397	50	3H-PYRILAMINE	-32	38	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	CHEMBL629	397	50	3H-PYRILAMINE	-32	38	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	DB00321	397	50	3H-PYRILAMINE	-32	38	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	1204	1901	114	3H-PYRILAMINE	-3	23	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-PYRILAMINE	-3	23	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-PYRILAMINE	-3	23	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-PYRILAMINE	-3	23	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-PYRILAMINE	-3	23	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-PYRILAMINE	-3	23	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	None	214372	0	3H-Histamine	-7585	6	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	1248	1396	27	3H-Histamine	-15	6	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	3077	1396	27	3H-Histamine	-15	6	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	90045	1396	27	3H-Histamine	-15	6	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	CHEMBL12344	1396	27	3H-Histamine	-15	6	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	11957573	1979	29	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1979	29	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1979	29	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1979	29	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	921599	19255	44	3H-Histamine	234	2	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	None
	CHEMBL129660	19255	44	3H-Histamine	234	2	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	None
	1204	1901	114	3H-Histamine	-3	23	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-Histamine	-3	23	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-Histamine	-3	23	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-Histamine	-3	23	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-Histamine	-3	23	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-Histamine	-3	23	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1267	3744	43	125I-iodophenpropit	-33	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3744	43	125I-iodophenpropit	-33	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3744	43	125I-iodophenpropit	-33	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3032915	15321	20	3H-Histamine	-1	6	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	3032915	15321	20	3H-N-alpha-methylhistamine	-1	6	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15321	20	3H-Histamine	-1	6	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15321	20	3H-N-alpha-methylhistamine	-1	6	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	135398737	944	89	3H-JNJ 7777120	-57	91	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	3H-JNJ 7777120	-57	91	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	3H-JNJ 7777120	-57	91	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	3H-JNJ 7777120	-57	91	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	3H-JNJ 7777120	-57	91	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	45073439	214396	0	3H-N-alpha-methylhistamine	-75	4	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@@H](CC1=CN=CN1)N.Br.Br	None
	1224	1404	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	3100	1404	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	8980	1404	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	916	1404	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	CHEMBL657	1404	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	DB01075	1404	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	2782	31784	62	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL1407943	31784	62	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL3216363	31784	62	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	1223	940	32	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	940	32	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	940	32	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	4761	13330	39	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1193	13330	39	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1580221	13330	39	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	1267	3744	43	3H-Histamine	-33	25	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3744	43	3H-Histamine	-33	25	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3744	43	3H-Histamine	-33	25	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3744	43	3H-Histamine	-33	25	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3744	43	3H-Histamine	-33	25	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3744	43	3H-Histamine	-33	25	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	115237	55296	113	None	-162	54	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	CHEMBL1621	55296	113	None	-162	54	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	2247	502	77	None	-117	41	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	249	502	77	None	-117	41	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	2603	502	77	None	-117	41	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	CHEMBL296419	502	77	None	-117	41	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	DB00637	502	77	None	-117	41	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	5625	215971	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	456	6	3	6	2.7	CC(C)NC1=C(N=CC=C1)N1CCN(CC1)C(=O)C1=CC2=C(N1)C=CC(NS(C)(=O)=O)=C2	None
	1613	2316	44	None	-112	44	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	205	2316	44	None	-112	44	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	3964	2316	44	None	-112	44	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	CHEMBL831	2316	44	None	-112	44	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	DB00408	2316	44	None	-112	44	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	16106	117643	28	None	-20	11	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	None
	CHEMBL340801	117643	28	None	-20	11	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	None
	4761	13330	39	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1193	13330	39	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1580221	13330	39	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	135398737	944	89	None	-54	91	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	None	-54	91	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	None	-54	91	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	None	-54	91	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	None	-54	91	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	100	3745	52	None	-56	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	2637	3745	52	None	-56	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	5452	3745	52	None	-56	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	CHEMBL479	3745	52	None	-56	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	DB00679	3745	52	None	-56	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	16362	3076	67	None	-562	29	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	2172	3076	67	None	-562	29	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	90	3076	67	None	-562	29	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	CHEMBL1423	3076	67	None	-562	29	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	DB01100	3076	67	None	-562	29	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	135398737	944	89	None	-44	91	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	None	-44	91	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	None	-44	91	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	None	-44	91	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	None	-44	91	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	103	4089	56	None	-89	53	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2875	4089	56	None	-89	53	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	5736	4089	56	None	-89	53	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL285802	4089	56	None	-89	53	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB09225	4089	56	None	-89	53	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2726	906	64	3H-PYRILAMINE	-70	72	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	906	64	3H-PYRILAMINE	-70	72	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	906	64	3H-PYRILAMINE	-70	72	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	906	64	3H-PYRILAMINE	-70	72	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	906	64	3H-PYRILAMINE	-70	72	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	2726	906	64	3H-Histamine	-70	72	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	906	64	3H-Histamine	-70	72	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	906	64	3H-Histamine	-70	72	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	906	64	3H-Histamine	-70	72	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	906	64	3H-Histamine	-70	72	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	1613	2316	44	3H-Histamine	-112	44	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	205	2316	44	3H-Histamine	-112	44	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	3964	2316	44	3H-Histamine	-112	44	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	CHEMBL831	2316	44	3H-Histamine	-112	44	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	DB00408	2316	44	3H-Histamine	-112	44	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	135398737	944	89	3H-Histamine	-57	91	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	3H-Histamine	-57	91	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	3H-Histamine	-57	91	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	3H-Histamine	-57	91	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	3H-Histamine	-57	91	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	11957573	1979	29	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1979	29	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1979	29	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1979	29	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	2782	31784	62	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL1407943	31784	62	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL3216363	31784	62	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	1210	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	1213	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2725	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	33036	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	4411	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	616	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	6976	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	716121	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	90475904	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1201353	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1554789	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL505	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB01114	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB13679	905	45	None	-186	20	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2286	3134	48	None	-10	29	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	4927	3134	48	None	-10	29	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	7282	3134	48	None	-10	29	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	CHEMBL643	3134	48	None	-10	29	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	DB01069	3134	48	None	-10	29	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	2726	906	64	None	-70	72	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	906	64	None	-70	72	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	906	64	None	-70	72	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	906	64	None	-70	72	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	906	64	None	-70	72	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	1204	1901	114	None	-15	23	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	None	-15	23	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	None	-15	23	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	None	-15	23	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	None	-15	23	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	None	-15	23	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1901	114	None	-12	23	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	None	-12	23	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	None	-12	23	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	None	-12	23	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	None	-12	23	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	None	-12	23	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1223	940	32	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1223	940	32	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	940	32	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	940	32	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	940	32	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	940	32	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	135	2496	38	3H-PYRILAMINE	-389	57	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1796	2496	38	3H-PYRILAMINE	-389	57	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	4184	2496	38	3H-PYRILAMINE	-389	57	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	CHEMBL6437	2496	38	3H-PYRILAMINE	-389	57	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	DB06148	2496	38	3H-PYRILAMINE	-389	57	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1267	3744	43	3H-JNJ 7777120	-33	25	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3744	43	3H-JNJ-5207852	-33	25	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3744	43	3H-JNJ 7777120	-33	25	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3744	43	3H-JNJ-5207852	-33	25	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3744	43	3H-JNJ 7777120	-33	25	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3744	43	3H-JNJ-5207852	-33	25	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1204	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1901	114	3H-Histamine	-3	23	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	3H-Histamine	-3	23	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	3H-Histamine	-3	23	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	3H-Histamine	-3	23	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	3H-Histamine	-3	23	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	3H-Histamine	-3	23	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	180	397	50	None	-32	38	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	200	397	50	None	-32	38	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	2160	397	50	None	-32	38	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	CHEMBL629	397	50	None	-32	38	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	DB00321	397	50	None	-32	38	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	277	1274	55	None	-301	45	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1274	55	None	-301	45	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1274	55	None	-301	45	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1274	55	None	-301	45	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1274	55	None	-301	45	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1204	1901	114	None	1	23	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1901	114	None	1	23	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1901	114	None	1	23	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1901	114	None	1	23	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1901	114	None	1	23	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1901	114	None	1	23	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	135398737	944	89	None	-57	91	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	None	-57	91	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	None	-57	91	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	None	-57	91	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	None	-57	91	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	135398737	944	89	3H-Histamine	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	3H-Histamine	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	3H-Histamine	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	3H-Histamine	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	3H-Histamine	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	135398737	944	89	3H-PYRILAMINE	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	944	89	3H-PYRILAMINE	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	944	89	3H-PYRILAMINE	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	944	89	3H-PYRILAMINE	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	944	89	3H-PYRILAMINE	-57	91	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	115237	55296	113	3H-Histamine	-162	54	Human	5.1	pKi	=	5.1	Binding	NoneNone	PDSP KiDatabase	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	CHEMBL1621	55296	113	3H-Histamine	-162	54	Human	5.1	pKi	=	5.1	Binding	NoneNone	PDSP KiDatabase	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	1251	1983	34	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1983	34	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1983	34	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	23650961	3785	18	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	9276	3785	18	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	CHEMBL3301609	3785	18	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	DB12522	3785	18	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	1265	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	1265	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	1265	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	6422124	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	6422124	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	6422124	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	CHEMBL14638	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	CHEMBL14638	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	CHEMBL14638	929	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	1267	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	1267	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	1267	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	1267	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1267	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	1267	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	3035905	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	3035905	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	3035905	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	3035905	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	3035905	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	CHEMBL260374	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	CHEMBL260374	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	CHEMBL260374	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	CHEMBL260374	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	CHEMBL260374	3744	43	None	-33	25	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	10446295	2101	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	1277	2101	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	CHEMBL185951	2101	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	1223	940	32	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	940	32	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	940	32	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1274	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	3063228	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	CHEMBL1196470	4001	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	1269	121	33	None	213	3	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	1269	121	33	None	213	3	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	37463	121	33	None	213	3	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	37463	121	33	None	213	3	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	CHEMBL275443	121	33	None	213	3	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	CHEMBL275443	121	33	None	213	3	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	1223	940	32	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	940	32	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	940	32	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1223	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	1223	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	1223	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1223	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	1223	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	2790	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	2790	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	2790	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	2790	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	CHEMBL14690	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	CHEMBL14690	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	CHEMBL14690	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	CHEMBL14690	940	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	24994634	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	24994634	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	8984	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	8984	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	CHEMBL3236549	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	CHEMBL3236549	2111	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	45257132	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	8983	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	CHEMBL595180	3626	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	1278	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1278	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	1278	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	1279	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1279	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	1279	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	4908365	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	4908365	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	4908365	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	CHEMBL129198	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	CHEMBL129198	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	CHEMBL129198	2119	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	117072554	1992	0	None	81	4	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	450	5	2	6	2.3	CN1CCN(CC1)C1=CC(=NC(N1)N)c1ccc2c(c1)CN(CC2)C(=O)CCC1CCCC1	26084539
	8982	1992	0	None	81	4	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	450	5	2	6	2.3	CN1CCN(CC1)C1=CC(=NC(N1)N)c1ccc2c(c1)CN(CC2)C(=O)CCC1CCCC1	26084539
	24745335	292	7	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	8985	292	7	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	CHEMBL1915540	292	7	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	DB15027	292	7	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	5048	3084	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	8924	3084	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	9948102	3084	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	CHEMBL462605	3084	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	DB11642	3084	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	441082	1140	40	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	8981	1140	40	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	CHEMBL191703	1140	40	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	11697697	2510	19	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	7346	2510	19	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	CHEMBL476323	2510	19	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	DB11910	2510	19	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	1547484	927	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	654	927	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	9072	927	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	CHEMBL43064	927	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	DB00568	927	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	10058807	1987	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	1276	1987	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	CHEMBL2261340	1987	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	135398737	944	89	None	-54	91	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	944	89	None	-54	91	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	944	89	None	-54	91	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	944	89	None	-54	91	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	944	89	None	-54	91	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	1238	2051	11	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	6603865	2051	11	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	CHEMBL11919	2051	11	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	1248	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	1248	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	1248	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	1248	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	3077	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	3077	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	3077	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	3077	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	90045	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	90045	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	90045	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	90045	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	CHEMBL12344	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	CHEMBL12344	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	CHEMBL12344	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	CHEMBL12344	1396	27	None	-15	6	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	135398737	944	89	None	-44	91	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	944	89	None	-44	91	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	944	89	None	-44	91	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	944	89	None	-44	91	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	944	89	None	-44	91	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	11313837	2464	7	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	1254	2464	7	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	CHEMBL175782	2464	7	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	124846	1915	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	1273	1915	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	CHEMBL67601	1915	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	12656	2009	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	1270	2009	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	CHEMBL12704	2009	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	1194	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	23786499	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	CHEMBL26116	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	1229	732	0	None	-19	6	Rat	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	732	0	None	-19	6	Rat	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1229	732	0	None	-15	6	Mouse	6.1	pKi	None	6.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	732	0	None	-15	6	Mouse	6.1	pKi	None	6.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1236	2036	23	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2036	23	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2036	23	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2036	23	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1241	2006	46	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	2006	46	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	2006	46	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	2006	46	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1268	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	15947036
	1268	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	16432504
	92613	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	15947036
	92613	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	16432504
	1236	2036	23	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2036	23	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2036	23	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2036	23	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	135398737	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	135398737	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	135398737	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	135398737	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	38	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	38	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	38	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	722	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	722	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	722	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	CHEMBL42	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	CHEMBL42	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	CHEMBL42	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	DB00363	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	DB00363	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	DB00363	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	944	89	None	-57	91	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	1241	2006	46	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	2006	46	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	2006	46	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	2006	46	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1236	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1236	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	1236	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	1236	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1237	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	1237	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	1237	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	156615	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	156615	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	156615	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	CHEMBL268229	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	CHEMBL268229	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	CHEMBL268229	2036	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1271	2005	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	44292270	2005	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	CHEMBL46296	2005	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	1239	2002	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	1239	2002	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	1240	2002	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	1240	2002	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	912	2002	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	912	2002	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	CHEMBL268490	2002	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	CHEMBL268490	2002	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	1229	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179434
	1229	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179436
	1229	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11181941
	1229	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1229	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	15947036
	5311293	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179434
	5311293	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179436
	5311293	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11181941
	5311293	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	732	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	15947036
	1204	1901	114	None	-12	23	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1901	114	None	-12	23	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1901	114	None	-12	23	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1901	114	None	-12	23	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1901	114	None	-12	23	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1901	114	None	-12	23	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1901	114	None	-15	23	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1901	114	None	-15	23	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1901	114	None	-15	23	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1901	114	None	-15	23	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1901	114	None	-15	23	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1901	114	None	-15	23	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1241	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1241	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1241	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	1242	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1242	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	3614	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	3614	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	CHEMBL507	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	CHEMBL507	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	2006	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	1267	3744	43	None	-21	25	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1267	3744	43	None	-14	25	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3744	43	None	-21	25	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3744	43	None	-14	25	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3744	43	None	-21	25	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3744	43	None	-14	25	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1266	2026	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	3960	2026	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	5311186	2026	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	1226	1988	16	None	2	6	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	1226	1988	16	None	2	6	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	41376	1988	16	None	2	6	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	41376	1988	16	None	2	6	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	CHEMBL12608	1988	16	None	2	6	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	CHEMBL12608	1988	16	None	2	6	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	1251	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	1251	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	1251	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	3035842	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	3035842	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	3035842	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	CHEMBL18661	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	CHEMBL18661	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	CHEMBL18661	1983	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	1204	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1204	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1204	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1204	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1204	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1204	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1247	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1247	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1247	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1247	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1247	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1247	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1375	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1375	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1375	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1375	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1375	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1375	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	774	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	774	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	774	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	774	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	774	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	774	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	CHEMBL90	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	DB05381	1901	114	None	-3	23	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	11957573	1979	29	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	11957573	1979	29	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1979	29	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1979	29	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1979	29	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1979	29	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1979	29	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1979	29	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	11957573	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	11957573	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	11957573	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	11957573	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	1250	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	1250	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	1250	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	3692	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	3692	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	3692	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	CHEMBL19439	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	CHEMBL19439	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	CHEMBL19439	1979	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071

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