MRGPRX2 - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

7S8M

RESOLUTION

2.5 Å

REFERENCE

10.1038/S41586-021-04126-6

PUBLICATION DATE

Nov. 17, 2021

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

29 interactions (Click to see)

LIGANDS

Cortistatin 14 - Agonist pubchem

PHYSIOLOGICAL LIGANDS

PAMP-20



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar TM3 S 130 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 S 130 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 S 130 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL2 P 134 34x50 G.H5 I 344 G.H5.16
Van-der-waals ICL2 P 134 34x50 G.H5 I 344 G.H5.16
Hydrophobic ICL3 R 214 - G.H5 D 341 G.H5.13
Van-der-waals ICL3 R 214 - G.H5 D 341 G.H5.13
Hydrophobic ICL2 I 135 34x51 G.s2s3 D 193 G.s2s3.02
Ionic ICL3 R 214 - G.h4s6 E 318 G.h4s6.12
Polar ICL3 R 214 - G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL3 R 214 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 R 214 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL2 R 138 34x54 G.hns1 R 32 G.hns1.03
Hydrophobic TM6 L 221 6x33 G.H5 L 348 G.H5.20
Hydrophobic TM7 G 283 7x57 G.H5 F 354 G.H5.26
Van-der-waals TM7 G 283 7x57 G.H5 F 354 G.H5.26
Polar TM2 N 62 2x37 G.H5 D 350 G.H5.22
Hydrophobic TM3 S 130 3x53 G.H5 C 351 G.H5.23
Hydrophobic ICL2 P 134 34x50 G.H5 T 340 G.H5.12
Hydrophobic ICL3 L 216 - G.H5 I 344 G.H5.16
Polar ICL2 R 138 34x54 G.hns1 A 31 G.hns1.02
Hydrophobic TM6 T 224 6x36 G.H5 L 353 G.H5.25
Polar ICL2 R 138 34x54 G.S1 E 33 G.S1.01
Van-der-waals ICL2 R 138 34x54 G.S1 E 33 G.S1.01
Hydrophobic TM6 L 221 6x33 G.H5 F 354 G.H5.26
Polar ICL2 Y 137 34x53 G.H5 C 351 G.H5.23
Hydrophobic ICL2 Y 137 34x53 G.H5 C 351 G.H5.23
Van-der-waals ICL2 Y 137 34x53 G.H5 C 351 G.H5.23
Hydrophobic ICL3 L 216 - G.H5 L 348 G.H5.20
Hydrophobic ICL2 I 135 34x51 G.S3 L 194 G.S3.01
Van-der-waals ICL2 I 135 34x51 G.S3 L 194 G.S3.01
Hydrophobic TM3 R 127 3x50 G.H5 L 353 G.H5.25
Van-der-waals TM3 R 127 3x50 G.H5 L 353 G.H5.25
Polar ICL3 G 215 - G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL3 G 215 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 G 215 - G.h4s6 E 318 G.h4s6.12
Hydrophobic ICL2 Y 137 34x53 G.H5 N 347 G.H5.19
Polar ICL2 C 139 34x55 G.s2s3 D 193 G.s2s3.02
Hydrophobic ICL2 I 135 34x51 G.H5 I 343 G.H5.15
Van-der-waals ICL2 I 135 34x51 G.H5 I 343 G.H5.15
Hydrophobic TM2 F 64 2x39 G.H5 C 351 G.H5.23
Aromatic ICL3 R 214 - G.S6 Y 320 G.S6.02
Hydrophobic ICL3 R 214 - G.S6 Y 320 G.S6.02
Hydrophobic ICL2 R 138 34x54 G.H5 I 343 G.H5.15
Hydrophobic TM3 V 131 3x54 G.H5 I 344 G.H5.16
Polar TM3 R 127 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 127 3x50 G.H5 C 351 G.H5.23
Van-der-waals TM3 R 127 3x50 G.H5 C 351 G.H5.23
Polar ICL2 P 134 34x50 G.H5 N 347 G.H5.19
Hydrophobic TM6 L 221 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 L 221 6x33 G.H5 L 353 G.H5.25
Hydrophobic ICL2 P 134 34x50 G.H5 I 343 G.H5.15
Hydrophobic TM3 V 131 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 V 131 3x54 G.H5 L 348 G.H5.20
Hydrophobic ICL2 R 140 34x56 G.s2s3 D 193 G.s2s3.02
Hydrophobic TM5 L 205 5x61 G.H5 L 353 G.H5.25
Hydrophobic ICL3 L 216 - G.H5 K 345 G.H5.17
Polar ICL3 R 214 - G.S6 I 319 G.S6.01
Van-der-waals ICL3 R 214 - G.S6 I 319 G.S6.01
Polar ICL2 C 139 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 C 139 34x55 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 C 139 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 I 135 34x51 G.H5 F 336 G.H5.08