PAC1 receptor - Gs

GPCR CLASS (FAMILY)

Class B1 (Secretin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8E3X

RESOLUTION

2.3 Å

REFERENCE

10.1038/S41467-022-34629-3

PUBLICATION DATE

Nov. 23, 2022

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

107 interactions (Click to see)

LIGANDS

[Ac-His1]PACAP-27 - Agonist gtoplig chembl_ligand pubchem

PHYSIOLOGICAL LIGANDS

PACAP-27, PACAP-38, VIP, PHV, PHM, PHI



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL2 F 259 - G.H5 C 379 G.H5.11
Ionic ICL3 D 339 - G.H4 R 342 G.H4.12
Hydrophobic H8 N 404 8x47 G.H5 E 392 G.H5.24
Van-der-waals H8 N 404 8x47 G.H5 E 392 G.H5.24
Polar TM2 R 185 2x46 G.H5 Q 390 G.H5.22
Hydrophobic TM2 R 185 2x46 G.H5 Q 390 G.H5.22
Hydrophobic TM3 Y 250 3x53 G.H5 Y 391 G.H5.23
Van-der-waals TM3 Y 250 3x53 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 F 259 - G.S3 V 217 G.S3.01
Van-der-waals ICL2 F 259 - G.S3 V 217 G.S3.01
Hydrophobic ICL2 F 259 - G.H5 R 380 G.H5.12
Van-der-waals ICL2 F 259 - G.H5 R 380 G.H5.12
Hydrophobic TM5 L 331 5x61 G.H5 L 388 G.H5.20
Hydrophobic TM6 R 350 6x37 G.H5 L 394 G.H5.26
Polar ICL3 S 337 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 S 337 - G.H5 R 385 G.H5.17
Hydrophobic TM7 Y 400 7x57 G.H5 Y 391 G.H5.23
Hydrophobic TM3 L 251 3x54 G.H5 L 393 G.H5.25
Polar TM6 R 350 6x37 G.H5 L 393 G.H5.25
Hydrophobic TM6 R 350 6x37 G.H5 L 393 G.H5.25
Hydrophobic ICL2 F 259 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 F 259 - G.H5 I 383 G.H5.15
Hydrophobic ICL3 M 340 - G.S6 Y 360 G.S6.02
Polar TM5 K 334 5x64 G.H5 R 385 G.H5.17
Hydrophobic TM5 K 334 5x64 G.H5 R 385 G.H5.17
Van-der-waals TM5 K 334 5x64 G.H5 R 385 G.H5.17
Hydrophobic ICL3 M 340 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 M 340 - G.H5 R 385 G.H5.17
Hydrophobic TM5 L 331 5x61 G.H5 L 393 G.H5.25
Hydrophobic TM5 L 335 5x65 G.H5 L 394 G.H5.26
Hydrophobic ICL2 T 258 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 T 258 - G.H5 I 383 G.H5.15
Polar ICL2 T 258 - G.H5 Q 384 G.H5.16
Hydrophobic ICL2 T 258 - G.H5 Q 384 G.H5.16
Van-der-waals ICL2 T 258 - G.H5 Q 384 G.H5.16
Hydrophobic TM3 L 254 3x57 G.H5 Y 391 G.H5.23
Polar TM3 V 256 3x59 G.H5 R 380 G.H5.12
Polar ICL2 T 258 - G.H5 R 380 G.H5.12
Hydrophobic ICL2 T 258 - G.H5 R 380 G.H5.12
Van-der-waals ICL2 T 258 - G.H5 R 380 G.H5.12
Hydrophobic TM5 L 331 5x61 G.H5 L 394 G.H5.26
Van-der-waals TM5 L 331 5x61 G.H5 L 394 G.H5.26
Polar TM5 K 334 5x64 G.H5 Q 384 G.H5.16
Hydrophobic TM5 K 334 5x64 G.H5 Q 384 G.H5.16
Van-der-waals TM5 K 334 5x64 G.H5 Q 384 G.H5.16
Ionic TM5 K 334 5x64 G.H5 D 381 G.H5.13
Polar TM5 K 334 5x64 G.H5 D 381 G.H5.13
Hydrophobic TM5 K 334 5x64 G.H5 D 381 G.H5.13
Van-der-waals TM5 K 334 5x64 G.H5 D 381 G.H5.13
Aromatic ICL2 F 259 - G.S1 H 41 G.S1.02
Hydrophobic ICL2 F 259 - G.S1 H 41 G.S1.02
Van-der-waals ICL2 F 259 - G.S1 H 41 G.S1.02
Hydrophobic TM6 L 358 6x45 G.H5 L 393 G.H5.25
Polar H8 G 405 8x48 G.H5 E 392 G.H5.24
Van-der-waals H8 G 405 8x48 G.H5 E 392 G.H5.24
Polar TM3 L 255 3x58 G.H5 Q 384 G.H5.16
Hydrophobic TM3 L 255 3x58 G.H5 Q 384 G.H5.16
Van-der-waals TM3 L 255 3x58 G.H5 Q 384 G.H5.16
Polar TM6 S 354 6x41 G.H5 L 393 G.H5.25
Van-der-waals TM6 S 354 6x41 G.H5 L 393 G.H5.25
Hydrophobic TM3 L 251 3x54 G.H5 Y 391 G.H5.23
Van-der-waals TM3 L 251 3x54 G.H5 Y 391 G.H5.23
Hydrophobic TM3 L 254 3x57 G.H5 H 387 G.H5.19
Aromatic TM2 R 185 2x46 G.H5 Y 391 G.H5.23
Hydrophobic TM2 R 185 2x46 G.H5 Y 391 G.H5.23
Van-der-waals TM2 R 185 2x46 G.H5 Y 391 G.H5.23
Hydrophobic ICL3 D 339 - G.H4 L 346 G.H4.16
Van-der-waals ICL3 D 339 - G.H4 L 346 G.H4.16
Hydrophobic TM3 L 255 3x58 G.H5 L 388 G.H5.20
Van-der-waals TM3 L 255 3x58 G.H5 L 388 G.H5.20
Hydrophobic ICL3 M 340 - G.h4s6 Y 358 G.h4s6.20
Van-der-waals ICL3 M 340 - G.h4s6 Y 358 G.h4s6.20
Hydrophobic ICL2 F 259 - G.S3 F 219 G.S3.03
Hydrophobic TM6 L 357 6x44 G.H5 L 393 G.H5.25
Hydrophobic TM5 K 334 5x64 G.H5 L 388 G.H5.20
Aromatic ICL2 F 259 - G.H5 F 376 G.H5.08
Hydrophobic ICL2 F 259 - G.H5 F 376 G.H5.08
Van-der-waals ICL2 F 259 - G.H5 F 376 G.H5.08
Hydrophobic TM2 H 189 2x50 G.H5 Y 391 G.H5.23
Polar TM6 R 350 6x37 G.H5 E 392 G.H5.24