VPAC1 receptor - Gs

GPCR CLASS (FAMILY)

Class B1 (Secretin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8E3Z

RESOLUTION

2.7 Å

REFERENCE

10.1038/S41467-022-34629-3

PUBLICATION DATE

Nov. 23, 2022

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

80 interactions (Click to see)

LIGANDS

Vasoactive intestinal polypeptide - Agonist pubchem chembl_ligand pubchem drugbank

PHYSIOLOGICAL LIGANDS

PHM, VIP, PACAP-38, PACAP-27, GHRH, PHI, secretin



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Ionic TM5 K 322 5x64 G.H5 D 381 G.H5.13
Hydrophobic TM5 K 322 5x64 G.H5 D 381 G.H5.13
Van-der-waals TM5 K 322 5x64 G.H5 D 381 G.H5.13
Polar TM6 S 342 6x41 G.H5 L 393 G.H5.25
Aromatic ICL2 F 248 - G.S1 H 41 G.S1.02
Hydrophobic ICL2 F 248 - G.S1 H 41 G.S1.02
Polar TM3 A 245 3x59 G.H5 R 380 G.H5.12
Van-der-waals TM3 A 245 3x59 G.H5 R 380 G.H5.12
Aromatic ICL2 F 248 - G.H5 R 380 G.H5.12
Hydrophobic ICL2 F 248 - G.H5 R 380 G.H5.12
Hydrophobic TM3 L 243 3x57 G.H5 H 387 G.H5.19
Van-der-waals TM3 L 243 3x57 G.H5 H 387 G.H5.19
Hydrophobic ICL3 I 328 - G.H4 L 346 G.H4.16
Hydrophobic TM3 L 243 3x57 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 F 248 - G.S3 F 219 G.S3.03
Polar TM2 R 174 2x46 G.H5 Q 390 G.H5.22
Hydrophobic TM2 R 174 2x46 G.H5 Q 390 G.H5.22
Van-der-waals TM2 R 174 2x46 G.H5 Q 390 G.H5.22
Hydrophobic ICL3 D 327 - G.S6 P 361 G.S6.03
Van-der-waals ICL3 D 327 - G.S6 P 361 G.S6.03
Polar ICL2 S 247 - G.H5 Q 384 G.H5.16
Hydrophobic ICL2 S 247 - G.H5 Q 384 G.H5.16
Van-der-waals ICL2 S 247 - G.H5 Q 384 G.H5.16
Hydrophobic TM5 K 322 5x64 G.H5 L 388 G.H5.20
Van-der-waals TM5 K 322 5x64 G.H5 L 388 G.H5.20
Hydrophobic TM6 L 346 6x45 G.H5 L 393 G.H5.25
Polar ICL3 R 329 - G.hgh4 D 323 G.hgh4.13
Hydrophobic ICL3 R 329 - G.hgh4 D 323 G.hgh4.13
Van-der-waals ICL3 R 329 - G.hgh4 D 323 G.hgh4.13
Aromatic TM2 R 174 2x46 G.H5 Y 391 G.H5.23
Hydrophobic TM2 R 174 2x46 G.H5 Y 391 G.H5.23
Van-der-waals TM2 R 174 2x46 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 F 248 - G.H5 C 379 G.H5.11
Polar TM5 K 322 5x64 G.H5 Q 384 G.H5.16
Hydrophobic TM5 K 322 5x64 G.H5 Q 384 G.H5.16
Van-der-waals TM5 K 322 5x64 G.H5 Q 384 G.H5.16
Ionic ICL3 D 327 - G.H4 R 342 G.H4.12
Hydrophobic ICL2 F 248 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 F 248 - G.H5 I 383 G.H5.15
Polar TM5 K 322 5x64 G.H5 R 385 G.H5.17
Hydrophobic TM5 K 322 5x64 G.H5 R 385 G.H5.17
Hydrophobic TM3 Y 239 3x53 G.H5 Y 391 G.H5.23
Van-der-waals TM3 Y 239 3x53 G.H5 Y 391 G.H5.23
Hydrophobic TM5 L 323 5x65 G.H5 L 394 G.H5.26
Hydrophobic TM3 L 240 3x54 G.H5 Y 391 G.H5.23
Van-der-waals TM3 L 240 3x54 G.H5 Y 391 G.H5.23
Polar ICL2 S 247 - G.H5 R 380 G.H5.12
Hydrophobic ICL2 S 247 - G.H5 R 380 G.H5.12
Van-der-waals ICL2 S 247 - G.H5 R 380 G.H5.12
Hydrophobic TM3 L 240 3x54 G.H5 L 393 G.H5.25
Hydrophobic ICL2 S 247 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 S 247 - G.H5 I 383 G.H5.15
Aromatic ICL2 F 248 - G.H5 F 376 G.H5.08
Hydrophobic ICL2 F 248 - G.H5 F 376 G.H5.08
Van-der-waals ICL2 F 248 - G.H5 F 376 G.H5.08
Hydrophobic TM5 L 319 5x61 G.H5 L 394 G.H5.26
Hydrophobic H8 N 392 8x47 G.H5 E 392 G.H5.24
Hydrophobic TM6 L 345 6x44 G.H5 L 393 G.H5.25
Van-der-waals TM6 L 345 6x44 G.H5 L 393 G.H5.25
Hydrophobic TM4 R 252 4x40 G.HN Q 35 G.HN.52
Van-der-waals TM4 R 252 4x40 G.HN Q 35 G.HN.52
Hydrophobic TM3 L 244 3x58 G.H5 L 388 G.H5.20
Van-der-waals TM3 L 244 3x58 G.H5 L 388 G.H5.20
Polar H8 G 393 8x48 G.H5 E 392 G.H5.24
Van-der-waals H8 G 393 8x48 G.H5 E 392 G.H5.24
Hydrophobic TM5 L 319 5x61 G.H5 L 388 G.H5.20
Hydrophobic ICL3 I 328 - G.H4 R 342 G.H4.12
Van-der-waals ICL3 I 328 - G.H4 R 342 G.H4.12
Polar TM3 L 244 3x58 G.H5 Q 384 G.H5.16
Hydrophobic TM3 L 244 3x58 G.H5 Q 384 G.H5.16
Van-der-waals TM3 L 244 3x58 G.H5 Q 384 G.H5.16
Hydrophobic ICL2 F 248 - G.S3 V 217 G.S3.01
Van-der-waals ICL2 F 248 - G.S3 V 217 G.S3.01
Hydrophobic ICL3 D 327 - G.H4 L 346 G.H4.16
Van-der-waals ICL3 D 327 - G.H4 L 346 G.H4.16
Hydrophobic ICL3 P 326 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 P 326 - G.H5 R 385 G.H5.17