EP3 receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8GDC

RESOLUTION

3.5 Å

REFERENCE

10.1073/PNAS.2216329120

PUBLICATION DATE

Jan. 10, 2024

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

39 interactions (Click to see)

LIGANDS

PGE2 - Agonist pubchem gtoplig chembl_ligand drugbank drug_central gtoplig

PHYSIOLOGICAL LIGANDS

PGE1, PGI2, thromboxane A2, PGF2α, PGD2, PGE2



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM3 I 159 3x54 G.H5 I 344 G.H5.16
Polar TM3 R 155 3x50 G.H5 G 352 G.H5.24
Ionic TM5 R 259 5x68 G.H5 D 337 G.H5.09
Polar TM5 R 259 5x68 G.H5 D 337 G.H5.09
Hydrophobic TM6 E 279 6x32 G.H5 F 354 G.H5.26
Van-der-waals TM6 E 279 6x32 G.H5 F 354 G.H5.26
Hydrophobic TM3 A 158 3x53 G.H5 L 348 G.H5.20
Ionic TM5 R 259 5x68 G.H5 D 341 G.H5.13
Hydrophobic TM3 E 154 3x49 G.H5 C 351 G.H5.23
Hydrophobic TM3 I 159 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 I 159 3x54 G.H5 L 348 G.H5.20
Hydrophobic ICL2 H 163 34x51 G.H5 I 343 G.H5.15
Hydrophobic ICL2 H 163 34x51 G.S3 L 194 G.S3.01
Hydrophobic TM3 R 155 3x50 G.H5 L 353 G.H5.25
Hydrophobic ICL2 S 167 34x55 G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 S 167 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic TM6 I 276 6x29 G.H5 L 348 G.H5.20
Polar TM6 Q 283 6x36 G.H5 L 353 G.H5.25
Van-der-waals TM6 Q 283 6x36 G.H5 L 353 G.H5.25
Hydrophobic TM6 L 284 6x37 G.H5 L 353 G.H5.25
Van-der-waals TM6 L 284 6x37 G.H5 L 353 G.H5.25
Hydrophobic ICL2 P 162 34x50 G.H5 T 340 G.H5.12
Polar ICL2 P 162 34x50 G.H5 N 347 G.H5.19
Van-der-waals ICL2 P 162 34x50 G.H5 N 347 G.H5.19
Hydrophobic ICL2 Y 165 34x53 G.H5 N 347 G.H5.19
Van-der-waals ICL2 Y 165 34x53 G.H5 N 347 G.H5.19
Van-der-waals ICL2 S 167 34x55 G.HN E 28 G.HN.52
Polar TM3 A 158 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 A 158 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL1 K 86 - G.H5 D 350 G.H5.22
Van-der-waals ICL1 K 86 - G.H5 D 350 G.H5.22
Hydrophobic ICL2 Y 165 34x53 G.H5 D 350 G.H5.22
Hydrophobic TM2 F 88 2x39 G.H5 C 351 G.H5.23
Van-der-waals TM2 F 88 2x39 G.H5 C 351 G.H5.23
Hydrophobic TM2 F 88 2x39 G.H5 G 352 G.H5.24
Hydrophobic TM3 R 155 3x50 G.H5 C 351 G.H5.23
Van-der-waals TM3 R 155 3x50 G.H5 C 351 G.H5.23
Hydrophobic ICL2 P 162 34x50 G.H5 I 344 G.H5.16
Van-der-waals ICL2 P 162 34x50 G.H5 I 344 G.H5.16
Hydrophobic TM6 I 276 6x29 G.H5 F 354 G.H5.26
Hydrophobic TM6 T 280 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 T 280 6x33 G.H5 L 353 G.H5.25
Hydrophobic TM6 W 273 6x26 G.H5 L 348 G.H5.20
Polar ICL2 H 163 34x51 G.H5 T 340 G.H5.12
Hydrophobic ICL2 H 163 34x51 G.H5 T 340 G.H5.12
Van-der-waals ICL2 H 163 34x51 G.H5 T 340 G.H5.12
Polar ICL3 Q 272 - G.H5 K 345 G.H5.17
Hydrophobic TM6 W 273 6x26 G.H5 K 345 G.H5.17
Van-der-waals TM6 W 273 6x26 G.H5 K 345 G.H5.17
Polar ICL3 Q 272 - G.h4s6 D 315 G.h4s6.09
Hydrophobic ICL3 Q 272 - G.h4s6 D 315 G.h4s6.09
Van-der-waals ICL3 Q 272 - G.h4s6 D 315 G.h4s6.09
Hydrophobic TM6 W 273 6x26 G.H5 I 344 G.H5.16
Van-der-waals TM6 W 273 6x26 G.H5 I 344 G.H5.16