adrb2_human
NAME
3PDS
ORGANISM
Human (Homo sapiens)
PROTEIN NAME
adrb2_human
SOURCE
SWISSPROT
SEQUENCE
Perspective:
Wild-type
Construct
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N-term | |||||||||
M
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G
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Q
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P
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G
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N
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G
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S
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A
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F
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10 |
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L
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L
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A
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P
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N
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G
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S
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H
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A
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P
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20 |
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|||||
TM1 | |||||||||
D
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H
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D
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V
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T
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Q
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E
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R
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D
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E
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30 |
V
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W
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V
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V
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G
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M
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G
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I
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V
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M
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40 |
S
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L
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I
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V
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L
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A
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I
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V
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F
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G
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50 |
N
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V
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L
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V
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I
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T
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A
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I
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A
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K
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60 |
ICL1 | TM2 | ||||||||
F
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E
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R
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L
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Q
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T
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V
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T
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N
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Y
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70 |
F
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I
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T
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S
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L
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A
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C
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A
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D
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L
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80 |
V
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M
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G
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L
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A
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V
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V
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P
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F
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G
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90 |
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|||||||||
A
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A
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H
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I
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L
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M
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K
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M
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W
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T
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100 |
ECL1 | TM3 | ||||||||
F
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G
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N
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F
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W
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C
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E
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F
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W
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T
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110 |
S
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I
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D
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V
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L
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C
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V
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T
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A
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S
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120 |
I
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E
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T
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L
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C
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V
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I
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A
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V
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D
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130 |
R
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Y
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F
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A
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I
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T
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S
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P
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F
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K
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140 |
ICL2 | TM4 | ||||||||
Y
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Q
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S
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L
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L
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T
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K
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N
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K
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A
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150 |
R
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V
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I
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I
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L
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M
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V
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W
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I
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V
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160 |
S
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G
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L
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T
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S
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F
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L
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P
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I
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Q
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170 |
ECL2 | |||||||||
M
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H
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W
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Y
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R
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A
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T
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H
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Q
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E
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180 |
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|||||||||
A
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I
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N
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C
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Y
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A
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N
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E
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T
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C
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190 |
TM5 | |||||||||
C
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D
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F
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F
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T
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N
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Q
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A
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Y
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A
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200 |
I
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A
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S
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S
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I
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V
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S
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F
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Y
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V
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210 |
P
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L
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V
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I
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M
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V
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F
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V
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Y
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S
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220 |
R
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V
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F
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Q
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E
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A
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K
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R
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Q
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L
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230 |
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Q
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K
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I
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D
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K
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S
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E
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G
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R
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F
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240 |
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ICL3 | |||||||||
H
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V
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Q
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N
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L
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S
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Q
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V
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E
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Q
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250 |
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D
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G
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R
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T
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G
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H
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G
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L
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R
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R
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260 |
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TM6 | |||||||||
S
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S
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K
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F
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C
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L
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K
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E
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H
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K
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270 |
A
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L
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K
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T
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L
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G
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I
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I
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M
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G
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280 |
T
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F
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T
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L
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C
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W
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L
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P
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F
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F
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290 |
I
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V
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N
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I
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V
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H
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V
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I
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Q
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D
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300 |
ECL3 | TM7 | ||||||||
N
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L
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I
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R
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K
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E
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V
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Y
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I
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L
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310 |
L
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N
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W
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I
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G
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Y
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V
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N
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S
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G
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320 |
H8 | |||||||||
F
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N
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P
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L
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I
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Y
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C
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R
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S
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P
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330 |
D
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F
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R
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I
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A
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F
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Q
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E
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L
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L
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340 |
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C-term | |||||||||
C
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L
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R
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R
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S
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S
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L
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K
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A
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Y
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350 |
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|
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G
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N
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G
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Y
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S
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S
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N
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G
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N
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T
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360 |
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|
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G
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E
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Q
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S
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G
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Y
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H
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V
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E
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Q
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370 |
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E
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K
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E
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N
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K
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L
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L
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C
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E
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D
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380 |
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|
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L
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P
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G
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T
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E
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D
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F
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V
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G
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H
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390 |
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|
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Q
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G
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T
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V
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P
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S
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D
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N
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I
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D
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400 |
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|
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S
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Q
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G
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R
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N
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C
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S
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T
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N
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D
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410 |
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|
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S
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L
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L
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||||||||
N-term | TM1 | ||||||||
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T
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Q
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Q
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R
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D
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E
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V
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W
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V
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33 |
V
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G
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M
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G
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I
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V
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M
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S
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L
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I
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43 |
V
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L
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A
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I
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V
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F
|
G
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N
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V
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L
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53 |
V
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I
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T
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A
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I
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A
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K
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F
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E
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R
|
63 |
ICL1 | TM2 | ||||||||
L
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Q
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T
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V
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T
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N
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Y
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F
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I
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T
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73 |
S
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L
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A
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C
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A
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D
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L
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V
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M
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G
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83 |
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|||||||||
L
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A
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V
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V
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P
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F
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G
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A
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A
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C
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93 |
ECL1 | |||||||||
I
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L
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M
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K
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M
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W
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T
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F
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G
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N
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103 |
TM3 | |||||||||
F
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W
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C
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E
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F
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W
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T
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S
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I
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D
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113 |
V
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L
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C
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V
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T
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A
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S
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I
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E
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T
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123 |
L
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C
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V
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I
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A
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V
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D
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R
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Y
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F
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133 |
ICL2 | |||||||||
A
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I
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T
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S
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P
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F
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K
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Y
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Q
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S
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143 |
TM4 | |||||||||
L
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L
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T
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K
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N
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K
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A
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R
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V
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I
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153 |
I
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L
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M
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V
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W
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I
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V
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S
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G
|
L
|
163 |
T
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S
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F
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L
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P
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I
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Q
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M
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H
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W
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173 |
ECL2 | |||||||||
Y
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R
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A
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T
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H
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Q
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E
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A
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I
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N
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183 |
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|||||||||
C
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Y
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A
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E
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E
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T
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C
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C
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D
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F
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193 |
TM5 | |||||||||
F
|
T
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N
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Q
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A
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Y
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A
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I
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A
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S
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203 |
S
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I
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V
|
S
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F
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Y
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V
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P
|
L
|
V
|
213 |
I
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M
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V
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F
|
V
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Y
|
S
|
R
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V
|
F
|
223 |
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|||||||||
Q
|
E
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A
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K
|
R
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Q
|
L
|
|
F
|
C
|
265 |
TM6 | |||||||||
L
|
K
|
E
|
H
|
K
|
A
|
L
|
K
|
T
|
L
|
275 |
G
|
I
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I
|
M
|
G
|
T
|
F
|
T
|
L
|
C
|
285 |
W
|
L
|
P
|
F
|
F
|
I
|
V
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N
|
I
|
V
|
295 |
ECL3 | |||||||||
H
|
V
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I
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Q
|
D
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N
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L
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I
|
R
|
K
|
305 |
TM7 | |||||||||
E
|
V
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Y
|
I
|
L
|
L
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N
|
W
|
I
|
G
|
315 |
Y
|
V
|
N
|
S
|
G
|
F
|
N
|
P
|
L
|
I
|
325 |
H8 | |||||||||
Y
|
C
|
R
|
S
|
P
|
D
|
F
|
R
|
I
|
A
|
335 |
F
|
Q
|
E
|
L
|
L
|
C
|
L
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R
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R
|
S
|
345 |
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|||||
C-term | |||||
S
|
L
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K
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||
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His(6) | |||||||||
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SCHEMATIC
Perspective:
Wild-type
Construct
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DIAGRAMS
MUTATIONS
Position | Wild-type | Mutated |
---|---|---|
27 | | E | Q |
93 | | H | C |
187 | | N | E |
DELETIONS
MODIFICATIONS
AUXILIARY PROTEINS
Protein_type: prot_cleavage
Name: TEV protease
Sequence: None
Deletions:
Positions: Within Receptor_0 23:23
Presence in Crystal:NO
Remarks: None
Protein_type: fusion
Name: T4 Lysozyme (T4L)
Sequence: None
Deletions:
Positions: Within Receptor_1 233:260
Presence in Crystal:YES
Remarks: None
Protein_type: tag
Name: His(6)
Sequence: None
Deletions:
Positions: C-term_2 None:None
Presence in Crystal:YES
Remarks: None
EXPRESSION
Expression method | Host cell type | Host cell | Remarks |
---|---|---|---|
Baculovirus | Sf9 | Insect | baculovirus transfer vector pVL1392+BestBac |
SOLUBILIZATION
Solvent type | Item | Concentration |
---|---|---|
detergent | DDM | 1.0 %w/v |
Remarks:
PURIFICATION
Purification type | Description |
---|---|
Alkylation by Iodoacetamide | None |
Proteolysis by TEV protease | None |
Detergent DDM exchanged with 0.1%(w/v)MNG-3 amphiphile | None |
Remarks:None
CRYSTALLIZATION
METHOD: in meso/LCP
TYPE: lipidic cubic phase (LCP) (monoolein (9.9 MAG))
PROTEIN CONCENTRATION: 50 mg/ml
Temperature: 20°C
ph : 6.7-6.7
Remarks:
Chemical lists
Type | Item | Concentration |
---|---|---|
Additional | ||
unknown | Li2SO4 | 200 mM |
unknown | MES,pH 6.7 | 100 mM |
unknown | 1,4-butandediol | 3.5 %v/v |
unknown | DMSO | 4 %(v/v) |
unknown | PEG400 | 24-27 %v/v |
Detergent | ||
detergent | LMNG | 0.1 % w/v |