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Ligand source activities (1 row/activity)

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Ligands (move mouse cursor over ligand name to see structure)					Receptor		Activity									Chemical information
Sel. page	Common name No matches found	GPCRdb ID No matches found	Reference ligand No matches found	Vendors	Species No matches found		Assay Type No matches found	Activity Type No matches found	Activity Relation No matches found	Activity Value	p-value (-log)	Fold selectivity	Tested GPCRs	Assay Description No matches found	Source No matches found	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles No matches found	DOI No matches found

Ligands (move mouse cursor over ligand name to see structure)					Receptor	Activity									Chemical information
Sel. page	Common name No matches found	GPCRdb ID No matches found	Reference ligand No matches found	Vendors	Species No matches found	Assay Type No matches found	Activity Type No matches found	Activity Relation No matches found	Activity Value	p-value (-log)	Fold selectivity	Tested GPCRs	Assay Description No matches found	Source No matches found	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles No matches found	DOI No matches found
	138107701	187570	None	33	Human	Functional	pEC50	=	9.5	9.5	1	7	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	360	8	3	3	3.5	CC#CCC(C)[C@H](O)/C=C/[C@@H]1[C@H]2C/C(=C/CCCC(=O)O)C[C@H]2C[C@H]1O	10.1016/j.ejmech.2022.114154
	5311181	187570	None	33	Human	Functional	pEC50	=	9.5	9.5	1	7	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	360	8	3	3	3.5	CC#CCC(C)[C@H](O)/C=C/[C@@H]1[C@H]2C/C(=C/CCCC(=O)O)C[C@H]2C[C@H]1O	10.1016/j.ejmech.2022.114154
	5311181.0	187570	None	33	Human	Functional	pEC50	=	9.5	9.5	1	7	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	360	8	3	3	3.5	CC#CCC(C)[C@H](O)/C=C/[C@@H]1[C@H]2C/C(=C/CCCC(=O)O)C[C@H]2C[C@H]1O	10.1016/j.ejmech.2022.114154
	CHEMBL494	187570	None	33	Human	Functional	pEC50	=	9.5	9.5	1	7	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	360	8	3	3	3.5	CC#CCC(C)[C@H](O)/C=C/[C@@H]1[C@H]2C/C(=C/CCCC(=O)O)C[C@H]2C[C@H]1O	10.1016/j.ejmech.2022.114154
	DB01088	187570	None	33	Human	Functional	pEC50	=	9.5	9.5	1	7	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	360	8	3	3	3.5	CC#CCC(C)[C@H](O)/C=C/[C@@H]1[C@H]2C/C(=C/CCCC(=O)O)C[C@H]2C[C@H]1O	10.1016/j.ejmech.2022.114154
	127052613	140293	None	0	Human	Functional	pEC50	=	8.9	8.9	-1	6	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	431	7	3	7	3.1	O=C(O)c1csc([C@H]2CC[C@H]3[C@H](C[C@@H](O)[C@@H]3/C=C/[C@@H](O)COc3ccccc3)O2)n1	10.1021/acsmedchemlett.5b00455
	CHEMBL3804978	140293	None	0	Human	Functional	pEC50	=	8.9	8.9	-1	6	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	431	7	3	7	3.1	O=C(O)c1csc([C@H]2CC[C@H]3[C@H](C[C@@H](O)[C@@H]3/C=C/[C@@H](O)COc3ccccc3)O2)n1	10.1021/acsmedchemlett.5b00455
	127052614	140313	None	0	Human	Functional	pEC50	=	6.8	6.8	-40	6	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	415	7	2	6	4.1	O=C(O)c1csc([C@H]2CC[C@H]3[C@H](C[C@@H](O)[C@@H]3/C=C/CCOc3ccccc3)O2)n1	10.1021/acsmedchemlett.5b00455
	CHEMBL3805176	140313	None	0	Human	Functional	pEC50	=	6.8	6.8	-40	6	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	415	7	2	6	4.1	O=C(O)c1csc([C@H]2CC[C@H]3[C@H](C[C@@H](O)[C@@H]3/C=C/CCOc3ccccc3)O2)n1	10.1021/acsmedchemlett.5b00455
	58681361	144769	None	0	Human	Functional	pEC50	=	5.7	5.7	-19	3	Agonist activity at human recombinant EP1 receptor expressed in HEK293 cells assessed as effect on calcium accumulation by Fluo-4 AM dye based FLIPR assayAgonist activity at human recombinant EP1 receptor expressed in HEK293 cells assessed as effect on calcium accumulation by Fluo-4 AM dye based FLIPR assay	ChEMBL	318	8	2	3	3.5	Cc1ccc([C@H]2[C@H](O)CC(=O)[C@@H]2CCCCCCC(=O)O)cc1	nan
	CHEMBL3908432	144769	None	0	Human	Functional	pEC50	=	5.7	5.7	-19	3	Agonist activity at human recombinant EP1 receptor expressed in HEK293 cells assessed as effect on calcium accumulation by Fluo-4 AM dye based FLIPR assayAgonist activity at human recombinant EP1 receptor expressed in HEK293 cells assessed as effect on calcium accumulation by Fluo-4 AM dye based FLIPR assay	ChEMBL	318	8	2	3	3.5	Cc1ccc([C@H]2[C@H](O)CC(=O)[C@@H]2CCCCCCC(=O)O)cc1	nan
	1955	16	None	0	Human	Functional	pEC50	=	4.7	4.7	-2089	5	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)	ChEMBL	428	13	4	5	3.6	O[C@@H](COc1cccc(c1)Cl)CC[C@H]1[C@H](O)C[C@@H]([C@@H]1CCCCCCC(=O)O)O	10.1016/s0960-894x(00)00273-0
	5311240	16	None	0	Human	Functional	pEC50	=	4.7	4.7	-2089	5	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)	ChEMBL	428	13	4	5	3.6	O[C@@H](COc1cccc(c1)Cl)CC[C@H]1[C@H](O)C[C@@H]([C@@H]1CCCCCCC(=O)O)O	10.1016/s0960-894x(00)00273-0
	CHEMBL36041	16	None	0	Human	Functional	pEC50	=	4.7	4.7	-2089	5	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)	ChEMBL	428	13	4	5	3.6	O[C@@H](COc1cccc(c1)Cl)CC[C@H]1[C@H](O)C[C@@H]([C@@H]1CCCCCCC(=O)O)O	10.1016/s0960-894x(00)00273-0
	56839536	143256	None	0	Human	Functional	pEC50	=	7.7	7.7	-1	7	Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.	ChEMBL	604	15	2	7	5.1	CCCCCCCCNC(=O)c1coc([C@@H]2CCCN2Cc2cc(F)ccc2CCC(=O)NS(=O)(=O)C(F)(F)F)n1	nan
	CHEMBL3896035	143256	None	0	Human	Functional	pEC50	=	7.7	7.7	-1	7	Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.	ChEMBL	604	15	2	7	5.1	CCCCCCCCNC(=O)c1coc([C@@H]2CCCN2Cc2cc(F)ccc2CCC(=O)NS(=O)(=O)C(F)(F)F)n1	nan
	56839342	149095	None	0	Human	Functional	pEC50	=	7.7	7.7	-1	7	Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.	ChEMBL	646	13	2	7	6.0	O=C(CCc1ccc(Cl)cc1CN1CCC[C@H]1c1nc(C(=O)NCCCCC2CCCCC2)co1)NS(=O)(=O)C(F)(F)F	nan
	CHEMBL3942394	149095	None	0	Human	Functional	pEC50	=	7.7	7.7	-1	7	Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.	ChEMBL	646	13	2	7	6.0	O=C(CCc1ccc(Cl)cc1CN1CCC[C@H]1c1nc(C(=O)NCCCCC2CCCCC2)co1)NS(=O)(=O)C(F)(F)F	nan
	145977227	164102	None	0	Human	Functional	pEC50	=	5.7	5.7	-1995	4	Agonist activity at recombinant human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level measured at 3 secs time interval by fura-2-AM dye based fluorescence assayAgonist activity at recombinant human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level measured at 3 secs time interval by fura-2-AM dye based fluorescence assay	ChEMBL	437	10	2	6	5.2	C[C@@](O)(C/C=C/[C@H]1CCC(=O)[C@@H]1CCSc1nc(C(=O)O)cs1)CC1CCCC1	10.1016/j.bmc.2017.11.035
	CHEMBL4208379	164102	None	0	Human	Functional	pEC50	=	5.7	5.7	-1995	4	Agonist activity at recombinant human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level measured at 3 secs time interval by fura-2-AM dye based fluorescence assayAgonist activity at recombinant human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level measured at 3 secs time interval by fura-2-AM dye based fluorescence assay	ChEMBL	437	10	2	6	5.2	C[C@@](O)(C/C=C/[C@H]1CCC(=O)[C@@H]1CCSc1nc(C(=O)O)cs1)CC1CCCC1	10.1016/j.bmc.2017.11.035
	11955358	153171	None	0	Human	Functional	pEC50	=	6.7	6.7	-1	3	Agonist activity at human recombinant EP1 receptor expressed in HEK293 cells assessed as effect on calcium accumulation by Fluo-4 AM dye based FLIPR assayAgonist activity at human recombinant EP1 receptor expressed in HEK293 cells assessed as effect on calcium accumulation by Fluo-4 AM dye based FLIPR assay	ChEMBL	364	8	2	2	4.7	O=C(O)CCCCCC[C@@H]1[C@@H](c2ccc3c(c2)CCC3)[C@H](O)C[C@H]1Cl	nan
	CHEMBL3976116	153171	None	0	Human	Functional	pEC50	=	6.7	6.7	-1	3	Agonist activity at human recombinant EP1 receptor expressed in HEK293 cells assessed as effect on calcium accumulation by Fluo-4 AM dye based FLIPR assayAgonist activity at human recombinant EP1 receptor expressed in HEK293 cells assessed as effect on calcium accumulation by Fluo-4 AM dye based FLIPR assay	ChEMBL	364	8	2	2	4.7	O=C(O)CCCCCC[C@@H]1[C@@H](c2ccc3c(c2)CCC3)[C@H](O)C[C@H]1Cl	nan
	156022045	178305	None	0	Human	Functional	pEC50	=	5.6	5.6	-4	3	Agonist activity at human EP1 receptor expressed in HEK293 cells by calcium-5 dye based FLIPR assayAgonist activity at human EP1 receptor expressed in HEK293 cells by calcium-5 dye based FLIPR assay	ChEMBL	351	14	2	4	3.8	CCCCCC(O)CCc1cccc(=O)n1CCCCCCC(=O)O	10.1016/j.bmcl.2020.127104
	CHEMBL4649582	178305	None	0	Human	Functional	pEC50	=	5.6	5.6	-4	3	Agonist activity at human EP1 receptor expressed in HEK293 cells by calcium-5 dye based FLIPR assayAgonist activity at human EP1 receptor expressed in HEK293 cells by calcium-5 dye based FLIPR assay	ChEMBL	351	14	2	4	3.8	CCCCCC(O)CCc1cccc(=O)n1CCCCCCC(=O)O	10.1016/j.bmcl.2020.127104
	2720	3854	None	49	Human	Functional	pEC50	=	6.5	6.5	-478	5	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	390	10	3	4	3.6	CCCCC[C@@H](CC[C@H]1[C@H](O)C[C@H]2[C@@H]1Cc1cccc(c1C2)OCC(=O)O)O	10.1016/j.ejmech.2022.114154
	5820	3854	None	49	Human	Functional	pEC50	=	6.5	6.5	-478	5	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	390	10	3	4	3.6	CCCCC[C@@H](CC[C@H]1[C@H](O)C[C@H]2[C@@H]1Cc1cccc(c1C2)OCC(=O)O)O	10.1016/j.ejmech.2022.114154
	6918140	3854	None	49	Human	Functional	pEC50	=	6.5	6.5	-478	5	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	390	10	3	4	3.6	CCCCC[C@@H](CC[C@H]1[C@H](O)C[C@H]2[C@@H]1Cc1cccc(c1C2)OCC(=O)O)O	10.1016/j.ejmech.2022.114154
	6918140.0	3854	None	49	Human	Functional	pEC50	=	6.5	6.5	-478	5	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	390	10	3	4	3.6	CCCCC[C@@H](CC[C@H]1[C@H](O)C[C@H]2[C@@H]1Cc1cccc(c1C2)OCC(=O)O)O	10.1016/j.ejmech.2022.114154
	CHEMBL1237119	3854	None	49	Human	Functional	pEC50	=	6.5	6.5	-478	5	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	390	10	3	4	3.6	CCCCC[C@@H](CC[C@H]1[C@H](O)C[C@H]2[C@@H]1Cc1cccc(c1C2)OCC(=O)O)O	10.1016/j.ejmech.2022.114154
	DB00374	3854	None	49	Human	Functional	pEC50	=	6.5	6.5	-478	5	Agonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysisAgonist activity at human EP1 expressed in HEK293 cells assessed as increase in cAMP level incubated for 20 mins measured after 60 mins addition of cAMP detect reagent by HTRF analysis	ChEMBL	390	10	3	4	3.6	CCCCC[C@@H](CC[C@H]1[C@H](O)C[C@H]2[C@@H]1Cc1cccc(c1C2)OCC(=O)O)O	10.1016/j.ejmech.2022.114154
	1883	3082	None	47	Human	Functional	pEC50	=	8.4	8.4	-1	12	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/acsmedchemlett.5b00455
	1916	3082	None	47	Human	Functional	pEC50	=	8.4	8.4	-1	12	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/acsmedchemlett.5b00455
	5280360	3082	None	47	Human	Functional	pEC50	=	8.4	8.4	-1	12	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/acsmedchemlett.5b00455
	5280360.0	3082	None	47	Human	Functional	pEC50	=	8.4	8.4	-1	12	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/acsmedchemlett.5b00455
	913	3082	None	47	Human	Functional	pEC50	=	8.4	8.4	-1	12	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/acsmedchemlett.5b00455
	CHEMBL548	3082	None	47	Human	Functional	pEC50	=	8.4	8.4	-1	12	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/acsmedchemlett.5b00455
	DB00917	3082	None	47	Human	Functional	pEC50	=	8.4	8.4	-1	12	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/acsmedchemlett.5b00455
	6441607	155169	None	22	Human	Functional	pEC50	=	6.5	6.5	-12	4	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)	ChEMBL	382	12	4	4	3.7	CCCCC(C)(C)[C@H](O)/C=C/[C@H]1[C@H](O)C[C@H](O)[C@@H]1C/C=C\CCCC(=O)O	10.1016/s0960-894x(00)00273-0
	CHEMBL40183	155169	None	22	Human	Functional	pEC50	=	6.5	6.5	-12	4	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)	ChEMBL	382	12	4	4	3.7	CCCCC(C)(C)[C@H](O)/C=C/[C@H]1[C@H](O)C[C@H](O)[C@@H]1C/C=C\CCCC(=O)O	10.1016/s0960-894x(00)00273-0
	10389527	153434	None	17	Human	Functional	pEC50	=	4.5	4.5	-1122	2	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)	ChEMBL	460	13	4	4	4.6	O=C(O)CCCCCC[C@H]1[C@@H](O)C[C@@H](O)[C@@H]1CC[C@@H](O)CCc1cccc(C(F)(F)F)c1	10.1016/s0960-894x(00)00273-0
	CHEMBL39784	153434	None	17	Human	Functional	pEC50	=	4.5	4.5	-1122	2	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=100%)	ChEMBL	460	13	4	4	4.6	O=C(O)CCCCCC[C@H]1[C@@H](O)C[C@@H](O)[C@@H]1CC[C@@H](O)CCc1cccc(C(F)(F)F)c1	10.1016/s0960-894x(00)00273-0
	126495398	140298	None	0	Human	Functional	pEC50	=	5.4	5.4	-416	3	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	403	7	2	6	3.9	O=C(O)c1csc([C@H]2CC[C@H]3[C@H](C[C@@H](O)[C@@H]3CCCOc3ccccc3)O2)n1	10.1021/acsmedchemlett.5b00455
	CHEMBL3805044	140298	None	0	Human	Functional	pEC50	=	5.4	5.4	-416	3	Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysisAgonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis	ChEMBL	403	7	2	6	3.9	O=C(O)c1csc([C@H]2CC[C@H]3[C@H](C[C@@H](O)[C@@H]3CCCOc3ccccc3)O2)n1	10.1021/acsmedchemlett.5b00455
	56649302	152755	None	0	Human	Functional	pEC50	=	7.3	7.3	-1	6	Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.	ChEMBL	630	13	2	7	5.5	O=C(CCc1ccc(F)cc1CN1CCC[C@H]1c1nc(C(=O)NCCCCC2CCCCC2)co1)NS(=O)(=O)C(F)(F)F	nan
	CHEMBL3972583	152755	None	0	Human	Functional	pEC50	=	7.3	7.3	-1	6	Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.Cell Based Assay: Ca2+ signaling studies were performed using a FLIPR TETRA system (Molecular Devices, Sunnyvale, Calif., USA) in the 384-format. This is a high-throughput instrument for cell-based assays to monitor Ca2+ signaling associated with GPCRs and ion channels. Cells were seeded at a density of 5×104 cells/well in BioCoat poly-D-lysine coated, black wall, clear bottom 384-well plates (BD Biosciences, Franklin lakes, NJ, USA) and allowed to attach overnight in an incubator at 37° C. The cells were then washed twice with HBSS-HEPES buffer (Hanks' balanced salt solution without bicarbonate and phenol red, 20 mM HEPES, pH 7.4) using an ELx405 Select CW Microplate Washer (BioTek, Winooski, Vt., USA). After 60 min of dye-loading in the dark using the Ca2+-sensitive dye Fluo-4AM (Invitrogen, Carlsbad, Calif., USA), at a final concentration of 2×10^−6M, the plates were washed 4 times with HBSS-HEPES buffer to remove excess dye and leaving 50 μl of buffer in each well. The plates were then placed in the FLIPR TETRA instrument and allowed to equilibrate at 37° C. AGN-211377 was added in a 25 μl volume to each well to give final concentrations of 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM, and 30 μM; or 0.067 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.67 μM, and 1 μM for cells over-expressing TP receptors. After 4.5 minutes, a 7-point serial dilution of the standard agonist for the corresponding receptor, in a 25 μl volume was injected at the final concentrations from 10^−11M to 10^−5M in 10-fold serial dilution increments for cells expressing human recombinant DP1, EP1, EP2, EP3, EP4, FP, and IP receptors. The dose range for the standard agonist for human recombinant TP receptors was from 10^−12M to 10^−6M. HBSS-HEPES buffer was used as the negative control for the standard agonists. Cells were excited with LED (light emitting diode) excitation at 470-495 nm and emission was measured through an emission filter at 515-575 nm. Assay plates were read for 3.5 minutes using the FLIPRTETRA.	ChEMBL	630	13	2	7	5.5	O=C(CCc1ccc(F)cc1CN1CCC[C@H]1c1nc(C(=O)NCCCCC2CCCCC2)co1)NS(=O)(=O)C(F)(F)F	nan
	1884	3083	None	40	Human	Functional	pEC50	=	6.2	6.2	-22	9	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)	ChEMBL	354	12	4	4	3.0	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)C[C@@H]([C@@H]1C/C=C\CCCC(=O)O)O)O	10.1016/s0960-894x(00)00273-0
	5280363	3083	None	40	Human	Functional	pEC50	=	6.2	6.2	-22	9	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)	ChEMBL	354	12	4	4	3.0	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)C[C@@H]([C@@H]1C/C=C\CCCC(=O)O)O)O	10.1016/s0960-894x(00)00273-0
	912	3083	None	40	Human	Functional	pEC50	=	6.2	6.2	-22	9	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)	ChEMBL	354	12	4	4	3.0	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)C[C@@H]([C@@H]1C/C=C\CCCC(=O)O)O)O	10.1016/s0960-894x(00)00273-0
	CHEMBL815	3083	None	40	Human	Functional	pEC50	=	6.2	6.2	-22	9	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)	ChEMBL	354	12	4	4	3.0	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)C[C@@H]([C@@H]1C/C=C\CCCC(=O)O)O)O	10.1016/s0960-894x(00)00273-0
	DB12789	3083	None	40	Human	Functional	pEC50	=	6.2	6.2	-22	9	Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)Functional activity in RAT-1cells, transiently-transfected with human Prostaglandin E receptor EP1 (% of control ligand, 17-phi-PGE2=80%)	ChEMBL	354	12	4	4	3.0	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)C[C@@H]([C@@H]1C/C=C\CCCC(=O)O)O)O	10.1016/s0960-894x(00)00273-0

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Ligands (move mouse cursor over ligand name to see structure)					Receptor		Activity									Chemical information
Sel. page	Common name No matches found	GPCRdb ID No matches found	Reference ligand No matches found	Vendors	Species No matches found		Assay Type No matches found	Activity Type No matches found	Activity Relation No matches found	Activity Value	p-value (-log)	Fold selectivity	Tested GPCRs	Assay Description No matches found	Source No matches found	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles No matches found	DOI No matches found

Ligands (move mouse cursor over ligand name to see structure)					Receptor	Activity									Chemical information
Sel. page	Common name No matches found	GPCRdb ID No matches found	Reference ligand No matches found	Vendors	Species No matches found	Assay Type No matches found	Activity Type No matches found	Activity Relation No matches found	Activity Value	p-value (-log)	Fold selectivity	Tested GPCRs	Assay Description No matches found	Source No matches found	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles No matches found	DOI No matches found
	10409554	149609	None	0	Human	Binding	pEC50	=	6.0	6.0	-	0	Radioligand Binding: HEK-293 cells stably expressing the human or feline FP receptor, or EP1, EP2, EP3, or EP4 receptors were washed with TME buffer, scraped from the bottom of the flasks, and homogenized for 30 sec using a Brinkman PT 10/35 polytron. TME buffer was added to achieve a final 40 ml volume in the centrifuge tubes (the composition of TME is 100 mM TRIS base, 20 mM MgCl2, 2M EDTA; 10 N HCl is added to achieve a pH of 7.4).The cell homogenate was centrifuged at 19000 r.p.m. for 20 min at 4 C. using a Beckman Ti-60 rotor. The resultant pellet was resuspended in TME buffer to give a final 1 mg/ml protein concentration, as determined by Biorad assay. Radioligand binding competition assays vs. [3H-]17-phenyl PGF2 (5 nM) were performed in a 100 ul volume for 60 min. Binding reactions were started by adding plasma membrane fraction. The reaction was terminated by the addition of 4 ml ice-cold TRIS-HCl buffer and rapid filtration through glass fiber GF/B filters using a Brandel cell.Radioligand Binding: HEK-293 cells stably expressing the human or feline FP receptor, or EP1, EP2, EP3, or EP4 receptors were washed with TME buffer, scraped from the bottom of the flasks, and homogenized for 30 sec using a Brinkman PT 10/35 polytron. TME buffer was added to achieve a final 40 ml volume in the centrifuge tubes (the composition of TME is 100 mM TRIS base, 20 mM MgCl2, 2M EDTA; 10 N HCl is added to achieve a pH of 7.4).The cell homogenate was centrifuged at 19000 r.p.m. for 20 min at 4 C. using a Beckman Ti-60 rotor. The resultant pellet was resuspended in TME buffer to give a final 1 mg/ml protein concentration, as determined by Biorad assay. Radioligand binding competition assays vs. [3H-]17-phenyl PGF2 (5 nM) were performed in a 100 ul volume for 60 min. Binding reactions were started by adding plasma membrane fraction. The reaction was terminated by the addition of 4 ml ice-cold TRIS-HCl buffer and rapid filtration through glass fiber GF/B filters using a Brandel cell.	ChEMBL	414	11	3	4	3.9	CC1(C)C(=O)[C@H](C/C=C\CCCC(=O)O)[C@@H](/C=C/C(O)CCc2ccccc2)[C@@H]1O	nan
	CHEMBL3946494	149609	None	0	Human	Binding	pEC50	=	6.0	6.0	-	0	Radioligand Binding: HEK-293 cells stably expressing the human or feline FP receptor, or EP1, EP2, EP3, or EP4 receptors were washed with TME buffer, scraped from the bottom of the flasks, and homogenized for 30 sec using a Brinkman PT 10/35 polytron. TME buffer was added to achieve a final 40 ml volume in the centrifuge tubes (the composition of TME is 100 mM TRIS base, 20 mM MgCl2, 2M EDTA; 10 N HCl is added to achieve a pH of 7.4).The cell homogenate was centrifuged at 19000 r.p.m. for 20 min at 4 C. using a Beckman Ti-60 rotor. The resultant pellet was resuspended in TME buffer to give a final 1 mg/ml protein concentration, as determined by Biorad assay. Radioligand binding competition assays vs. [3H-]17-phenyl PGF2 (5 nM) were performed in a 100 ul volume for 60 min. Binding reactions were started by adding plasma membrane fraction. The reaction was terminated by the addition of 4 ml ice-cold TRIS-HCl buffer and rapid filtration through glass fiber GF/B filters using a Brandel cell.Radioligand Binding: HEK-293 cells stably expressing the human or feline FP receptor, or EP1, EP2, EP3, or EP4 receptors were washed with TME buffer, scraped from the bottom of the flasks, and homogenized for 30 sec using a Brinkman PT 10/35 polytron. TME buffer was added to achieve a final 40 ml volume in the centrifuge tubes (the composition of TME is 100 mM TRIS base, 20 mM MgCl2, 2M EDTA; 10 N HCl is added to achieve a pH of 7.4).The cell homogenate was centrifuged at 19000 r.p.m. for 20 min at 4 C. using a Beckman Ti-60 rotor. The resultant pellet was resuspended in TME buffer to give a final 1 mg/ml protein concentration, as determined by Biorad assay. Radioligand binding competition assays vs. [3H-]17-phenyl PGF2 (5 nM) were performed in a 100 ul volume for 60 min. Binding reactions were started by adding plasma membrane fraction. The reaction was terminated by the addition of 4 ml ice-cold TRIS-HCl buffer and rapid filtration through glass fiber GF/B filters using a Brandel cell.	ChEMBL	414	11	3	4	3.9	CC1(C)C(=O)[C@H](C/C=C\CCCC(=O)O)[C@@H](/C=C/C(O)CCc2ccccc2)[C@@H]1O	nan
	10409554	149609	None	0	Human	Binding	pEC50	=	7.6	7.6	-	0	Radioligand Binding: HEK-293 cells stably expressing the human or feline FP receptor, or EP1, EP2, EP3, or EP4 receptors were washed with TME buffer, scraped from the bottom of the flasks, and homogenized for 30 sec using a Brinkman PT 10/35 polytron. TME buffer was added to achieve a final 40 ml volume in the centrifuge tubes (the composition of TME is 100 mM TRIS base, 20 mM MgCl2, 2M EDTA; 10 N HCl is added to achieve a pH of 7.4).The cell homogenate was centrifuged at 19000 r.p.m. for 20 min at 4 C. using a Beckman Ti-60 rotor. The resultant pellet was resuspended in TME buffer to give a final 1 mg/ml protein concentration, as determined by Biorad assay. Radioligand binding competition assays vs. [3H-]17-phenyl PGF2 (5 nM) were performed in a 100 ul volume for 60 min. Binding reactions were started by adding plasma membrane fraction. The reaction was terminated by the addition of 4 ml ice-cold TRIS-HCl buffer and rapid filtration through glass fiber GF/B filters using a Brandel cell.Radioligand Binding: HEK-293 cells stably expressing the human or feline FP receptor, or EP1, EP2, EP3, or EP4 receptors were washed with TME buffer, scraped from the bottom of the flasks, and homogenized for 30 sec using a Brinkman PT 10/35 polytron. TME buffer was added to achieve a final 40 ml volume in the centrifuge tubes (the composition of TME is 100 mM TRIS base, 20 mM MgCl2, 2M EDTA; 10 N HCl is added to achieve a pH of 7.4).The cell homogenate was centrifuged at 19000 r.p.m. for 20 min at 4 C. using a Beckman Ti-60 rotor. The resultant pellet was resuspended in TME buffer to give a final 1 mg/ml protein concentration, as determined by Biorad assay. Radioligand binding competition assays vs. [3H-]17-phenyl PGF2 (5 nM) were performed in a 100 ul volume for 60 min. Binding reactions were started by adding plasma membrane fraction. The reaction was terminated by the addition of 4 ml ice-cold TRIS-HCl buffer and rapid filtration through glass fiber GF/B filters using a Brandel cell.	ChEMBL	414	11	3	4	3.9	CC1(C)C(=O)[C@H](C/C=C\CCCC(=O)O)[C@@H](/C=C/C(O)CCc2ccccc2)[C@@H]1O	nan
	CHEMBL3946494	149609	None	0	Human	Binding	pEC50	=	7.6	7.6	-	0	Radioligand Binding: HEK-293 cells stably expressing the human or feline FP receptor, or EP1, EP2, EP3, or EP4 receptors were washed with TME buffer, scraped from the bottom of the flasks, and homogenized for 30 sec using a Brinkman PT 10/35 polytron. TME buffer was added to achieve a final 40 ml volume in the centrifuge tubes (the composition of TME is 100 mM TRIS base, 20 mM MgCl2, 2M EDTA; 10 N HCl is added to achieve a pH of 7.4).The cell homogenate was centrifuged at 19000 r.p.m. for 20 min at 4 C. using a Beckman Ti-60 rotor. The resultant pellet was resuspended in TME buffer to give a final 1 mg/ml protein concentration, as determined by Biorad assay. Radioligand binding competition assays vs. [3H-]17-phenyl PGF2 (5 nM) were performed in a 100 ul volume for 60 min. Binding reactions were started by adding plasma membrane fraction. The reaction was terminated by the addition of 4 ml ice-cold TRIS-HCl buffer and rapid filtration through glass fiber GF/B filters using a Brandel cell.Radioligand Binding: HEK-293 cells stably expressing the human or feline FP receptor, or EP1, EP2, EP3, or EP4 receptors were washed with TME buffer, scraped from the bottom of the flasks, and homogenized for 30 sec using a Brinkman PT 10/35 polytron. TME buffer was added to achieve a final 40 ml volume in the centrifuge tubes (the composition of TME is 100 mM TRIS base, 20 mM MgCl2, 2M EDTA; 10 N HCl is added to achieve a pH of 7.4).The cell homogenate was centrifuged at 19000 r.p.m. for 20 min at 4 C. using a Beckman Ti-60 rotor. The resultant pellet was resuspended in TME buffer to give a final 1 mg/ml protein concentration, as determined by Biorad assay. Radioligand binding competition assays vs. [3H-]17-phenyl PGF2 (5 nM) were performed in a 100 ul volume for 60 min. Binding reactions were started by adding plasma membrane fraction. The reaction was terminated by the addition of 4 ml ice-cold TRIS-HCl buffer and rapid filtration through glass fiber GF/B filters using a Brandel cell.	ChEMBL	414	11	3	4	3.9	CC1(C)C(=O)[C@H](C/C=C\CCCC(=O)O)[C@@H](/C=C/C(O)CCc2ccccc2)[C@@H]1O	nan
	11340370	80613	None	15	Human	Binding	pIC50	=	9.9	9.9	-	1	Displacement of [3H]PGE2 from human EP1 receptor expressed in CHO cellsDisplacement of [3H]PGE2 from human EP1 receptor expressed in CHO cells	ChEMBL	468	6	1	4	5.6	Cc1cc(C(=O)O)nn1Cc1cc(Br)ccc1OCc1ccc(Cl)cc1Cl	10.1016/j.bmcl.2009.02.112
	CHEMBL2110364	80613	None	15	Human	Binding	pIC50	=	9.9	9.9	-	1	Displacement of [3H]PGE2 from human EP1 receptor expressed in CHO cellsDisplacement of [3H]PGE2 from human EP1 receptor expressed in CHO cells	ChEMBL	468	6	1	4	5.6	Cc1cc(C(=O)O)nn1Cc1cc(Br)ccc1OCc1ccc(Cl)cc1Cl	10.1016/j.bmcl.2009.02.112
	CHEMBL214971	80613	None	15	Human	Binding	pIC50	=	9.9	9.9	-	1	Displacement of [3H]PGE2 from human EP1 receptor expressed in CHO cellsDisplacement of [3H]PGE2 from human EP1 receptor expressed in CHO cells	ChEMBL	468	6	1	4	5.6	Cc1cc(C(=O)O)nn1Cc1cc(Br)ccc1OCc1ccc(Cl)cc1Cl	10.1016/j.bmcl.2009.02.112
	11340370	80613	None	15	Human	Binding	pIC50	=	9.9	9.9	-	1	Displacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cellsDisplacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cells	ChEMBL	468	6	1	4	5.6	Cc1cc(C(=O)O)nn1Cc1cc(Br)ccc1OCc1ccc(Cl)cc1Cl	10.1016/j.bmcl.2006.06.086
	CHEMBL2110364	80613	None	15	Human	Binding	pIC50	=	9.9	9.9	-	1	Displacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cellsDisplacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cells	ChEMBL	468	6	1	4	5.6	Cc1cc(C(=O)O)nn1Cc1cc(Br)ccc1OCc1ccc(Cl)cc1Cl	10.1016/j.bmcl.2006.06.086
	CHEMBL214971	80613	None	15	Human	Binding	pIC50	=	9.9	9.9	-	1	Displacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cellsDisplacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cells	ChEMBL	468	6	1	4	5.6	Cc1cc(C(=O)O)nn1Cc1cc(Br)ccc1OCc1ccc(Cl)cc1Cl	10.1016/j.bmcl.2006.06.086
	10007859	166584	None	0	Human	Binding	pIC50	=	9.4	9.4	5011	2	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	554	7	2	4	6.7	CC(=O)Nc1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2F)c1	10.1016/j.bmcl.2006.10.078
	CHEMBL427844	166584	None	0	Human	Binding	pIC50	=	9.4	9.4	5011	2	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	554	7	2	4	6.7	CC(=O)Nc1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2F)c1	10.1016/j.bmcl.2006.10.078
	10459580	85707	None	0	Human	Binding	pIC50	=	9.4	9.4	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membraneDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membrane	ChEMBL	527	7	1	4	6.8	COc1ccc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2F)cc1C(=O)O	10.1016/j.bmcl.2006.11.059
	CHEMBL228586	85707	None	0	Human	Binding	pIC50	=	9.4	9.4	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membraneDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membrane	ChEMBL	527	7	1	4	6.8	COc1ccc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2F)cc1C(=O)O	10.1016/j.bmcl.2006.11.059
	44426669	142157	None	0	Human	Binding	pIC50	=	9.4	9.4	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membraneDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membrane	ChEMBL	554	7	2	4	6.7	CC(=O)Nc1ccc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2F)cc1C(=O)O	10.1016/j.bmcl.2006.11.059
	CHEMBL387969	142157	None	0	Human	Binding	pIC50	=	9.4	9.4	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membraneDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membrane	ChEMBL	554	7	2	4	6.7	CC(=O)Nc1ccc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2F)cc1C(=O)O	10.1016/j.bmcl.2006.11.059
	44430700	143982	None	0	Human	Binding	pIC50	=	9.3	9.3	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	512	6	2	4	6.4	Cc1ccc(-c2cc(Br)ccc2OCc2ccc(F)cc2F)n1-c1cc(N)cc(C(=O)O)c1	10.1016/j.bmcl.2006.10.078
	CHEMBL390189	143982	None	0	Human	Binding	pIC50	=	9.3	9.3	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	512	6	2	4	6.4	Cc1ccc(-c2cc(Br)ccc2OCc2ccc(F)cc2F)n1-c1cc(N)cc(C(=O)O)c1	10.1016/j.bmcl.2006.10.078
	11189804	80611	None	0	Human	Binding	pIC50	=	9.3	9.3	-	1	Displacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cellsDisplacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cells	ChEMBL	436	6	1	4	4.6	Cc1cc(C(=O)O)nn1Cc1cc(Br)ccc1OCc1ccc(F)cc1F	10.1016/j.bmcl.2006.06.086
	CHEMBL214967	80611	None	0	Human	Binding	pIC50	=	9.3	9.3	-	1	Displacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cellsDisplacement of [3H]PGE2 from EP1 receptor expressed in CHO-K1 cells	ChEMBL	436	6	1	4	4.6	Cc1cc(C(=O)O)nn1Cc1cc(Br)ccc1OCc1ccc(F)cc1F	10.1016/j.bmcl.2006.06.086
	59179887	105891	None	0	Human	Binding	pIC50	=	9.2	9.2	-	1	Antagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assayAntagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assay	ChEMBL	389	3	1	5	4.9	O=C(O)c1csc(-n2nc(-c3ccccc3)c3ccc(C(F)(F)F)cc32)n1	10.1016/j.bmcl.2014.01.052
	CHEMBL3127163	105891	None	0	Human	Binding	pIC50	=	9.2	9.2	-	1	Antagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assayAntagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assay	ChEMBL	389	3	1	5	4.9	O=C(O)c1csc(-n2nc(-c3ccccc3)c3ccc(C(F)(F)F)cc32)n1	10.1016/j.bmcl.2014.01.052
	10099424	142147	None	0	Human	Binding	pIC50	=	9.2	9.2	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membraneDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membrane	ChEMBL	563	8	1	4	7.4	Cc1ccc(-c2cc(Br)ccc2OCc2ccc(F)cc2F)n1-c1ccc(OC(F)F)c(C(=O)O)c1	10.1016/j.bmcl.2006.11.059
	CHEMBL387878	142147	None	0	Human	Binding	pIC50	=	9.2	9.2	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membraneDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membrane	ChEMBL	563	8	1	4	7.4	Cc1ccc(-c2cc(Br)ccc2OCc2ccc(F)cc2F)n1-c1ccc(OC(F)F)c(C(=O)O)c1	10.1016/j.bmcl.2006.11.059
	44430704	167867	None	0	Human	Binding	pIC50	=	9.1	9.1	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	536	7	2	4	6.6	CC(=O)Nc1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2)c1	10.1016/j.bmcl.2006.10.078
	CHEMBL430360	167867	None	0	Human	Binding	pIC50	=	9.1	9.1	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	536	7	2	4	6.6	CC(=O)Nc1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2)c1	10.1016/j.bmcl.2006.10.078
	10324022	85709	None	0	Human	Binding	pIC50	=	9.1	9.1	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membraneDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membrane	ChEMBL	508	6	2	4	6.5	Cc1c(N)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2)cc1C(=O)O	10.1016/j.bmcl.2006.11.059
	CHEMBL228593	85709	None	0	Human	Binding	pIC50	=	9.1	9.1	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membraneDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHOK1 cell membrane	ChEMBL	508	6	2	4	6.5	Cc1c(N)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2)cc1C(=O)O	10.1016/j.bmcl.2006.11.059
	59179305	103940	None	0	Human	Binding	pIC50	=	9.1	9.1	-	0	Antagonist activity human EP1 receptor assessed as inhibition of PGE2-induced effect by reporter gene assayAntagonist activity human EP1 receptor assessed as inhibition of PGE2-induced effect by reporter gene assay	ChEMBL	359	3	1	5	4.3	O=C(O)c1csc(-n2nc(-c3ccccc3)c3c2-c2ccccc2C3)n1	10.1016/j.bmcl.2013.10.065
	CHEMBL3092131	103940	None	0	Human	Binding	pIC50	=	9.1	9.1	-	0	Antagonist activity human EP1 receptor assessed as inhibition of PGE2-induced effect by reporter gene assayAntagonist activity human EP1 receptor assessed as inhibition of PGE2-induced effect by reporter gene assay	ChEMBL	359	3	1	5	4.3	O=C(O)c1csc(-n2nc(-c3ccccc3)c3c2-c2ccccc2C3)n1	10.1016/j.bmcl.2013.10.065
	68258993	105885	None	0	Human	Binding	pIC50	=	9	9.0	-	0	Antagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assayAntagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assay	ChEMBL	335	3	1	5	4.2	Cc1ccc2c(-c3ccccc3)nn(-c3nc(C(=O)O)cs3)c2c1	10.1016/j.bmcl.2014.01.052
	CHEMBL3127157	105885	None	0	Human	Binding	pIC50	=	9	9.0	-	0	Antagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assayAntagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assay	ChEMBL	335	3	1	5	4.2	Cc1ccc2c(-c3ccccc3)nn(-c3nc(C(=O)O)cs3)c2c1	10.1016/j.bmcl.2014.01.052
	10051605	79712	None	0	Human	Binding	pIC50	=	9	9.0	-	0	Inhibition of [3H]PGE2 binding to EP1 receptor expressed in CHO cellsInhibition of [3H]PGE2 binding to EP1 receptor expressed in CHO cells	ChEMBL	497	6	1	3	6.8	Cc1ccc(-c2cc(Br)ccc2OCc2c(F)cccc2F)n1-c1cccc(C(=O)O)c1	10.1016/j.bmcl.2006.04.073
	CHEMBL211534	79712	None	0	Human	Binding	pIC50	=	9	9.0	-	0	Inhibition of [3H]PGE2 binding to EP1 receptor expressed in CHO cellsInhibition of [3H]PGE2 binding to EP1 receptor expressed in CHO cells	ChEMBL	497	6	1	3	6.8	Cc1ccc(-c2cc(Br)ccc2OCc2c(F)cccc2F)n1-c1cccc(C(=O)O)c1	10.1016/j.bmcl.2006.04.073
	59179936	105887	None	0	Human	Binding	pIC50	=	9.0	9.0	-	0	Antagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assayAntagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assay	ChEMBL	351	4	1	6	3.9	COc1ccc2c(-c3ccccc3)nn(-c3nc(C(=O)O)cs3)c2c1	10.1016/j.bmcl.2014.01.052
	CHEMBL3127159	105887	None	0	Human	Binding	pIC50	=	9.0	9.0	-	0	Antagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assayAntagonist activity at human EP1 receptor assessed as inhibition of PGE-induced effect by reporter gene assay	ChEMBL	351	4	1	6	3.9	COc1ccc2c(-c3ccccc3)nn(-c3nc(C(=O)O)cs3)c2c1	10.1016/j.bmcl.2014.01.052
	1883	3082	None	47	Human	Binding	pIC50	=	9.0	9.0	-12	24	Binding affinity to EP1 receptorBinding affinity to EP1 receptor	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/jm9018756
	1916	3082	None	47	Human	Binding	pIC50	=	9.0	9.0	-12	24	Binding affinity to EP1 receptorBinding affinity to EP1 receptor	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/jm9018756
	5280360	3082	None	47	Human	Binding	pIC50	=	9.0	9.0	-12	24	Binding affinity to EP1 receptorBinding affinity to EP1 receptor	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/jm9018756
	5280360.0	3082	None	47	Human	Binding	pIC50	=	9.0	9.0	-12	24	Binding affinity to EP1 receptorBinding affinity to EP1 receptor	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/jm9018756
	913	3082	None	47	Human	Binding	pIC50	=	9.0	9.0	-12	24	Binding affinity to EP1 receptorBinding affinity to EP1 receptor	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/jm9018756
	CHEMBL548	3082	None	47	Human	Binding	pIC50	=	9.0	9.0	-12	24	Binding affinity to EP1 receptorBinding affinity to EP1 receptor	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/jm9018756
	DB00917	3082	None	47	Human	Binding	pIC50	=	9.0	9.0	-12	24	Binding affinity to EP1 receptorBinding affinity to EP1 receptor	ChEMBL	352	12	3	4	3.3	CCCCC[C@@H](/C=C/[C@H]1[C@H](O)CC(=O)[C@@H]1C/C=C\CCCC(=O)O)O	10.1021/jm9018756
	44430703	88082	None	0	Human	Binding	pIC50	=	8.9	8.9	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	518	7	2	4	6.5	CC(=O)Nc1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccccc2)c1	10.1016/j.bmcl.2006.10.078
	CHEMBL234519	88082	None	0	Human	Binding	pIC50	=	8.9	8.9	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	518	7	2	4	6.5	CC(=O)Nc1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccccc2)c1	10.1016/j.bmcl.2006.10.078
	44430706	88201	None	0	Human	Binding	pIC50	=	8.9	8.9	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	508	7	2	4	6.7	CC(=O)Nc1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(C(F)(F)F)ccc2OCc2ccccc2)c1	10.1016/j.bmcl.2006.10.078
	CHEMBL234727	88201	None	0	Human	Binding	pIC50	=	8.9	8.9	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	508	7	2	4	6.7	CC(=O)Nc1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(C(F)(F)F)ccc2OCc2ccccc2)c1	10.1016/j.bmcl.2006.10.078
	44430708	144033	None	0	Human	Binding	pIC50	=	8.9	8.9	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	568	7	1	4	6.8	CC(=O)N(C)c1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2F)c1	10.1016/j.bmcl.2006.10.078
	CHEMBL390238	144033	None	0	Human	Binding	pIC50	=	8.9	8.9	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	568	7	1	4	6.8	CC(=O)N(C)c1cc(C(=O)O)cc(-n2c(C)ccc2-c2cc(Br)ccc2OCc2ccc(F)cc2F)c1	10.1016/j.bmcl.2006.10.078
	44430710	166856	None	0	Human	Binding	pIC50	=	8.9	8.9	-	0	Displacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranesDisplacement of [3H]PGE2 from human recombinant EP1 receptor expressed in CHO cell membranes	ChEMBL	594	7	1	4	7.3	Cc1ccc(-c2cc(Br)ccc2OCc2ccc(F)cc2F)n1-c1cc(C(=O)O)cc(N2CCCCC2=O)c1	10.1016/j.bmcl.2006.10.078

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Ligand source activities (1 row/activity)