US28 - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Human cytomegalovirus

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

7RKM

RESOLUTION

3.5 Å

REFERENCE

10.1126/SCIADV.ABL5442

PUBLICATION DATE

Jan. 26, 2022

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

80 interactions (Click to see)

LIGANDS

Fractalkine - Agonist pubchem

PHYSIOLOGICAL LIGANDS

No physiological ligands available



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic TM4 V 141 4x39 G.HN R 24 G.HN.48
Van-der-waals TM4 V 141 4x39 G.HN R 24 G.HN.48
Hydrophobic ICL3 R 221 - G.h4s6 T 316 G.h4s6.10
Ionic ICL3 R 221 - G.h4s6 D 315 G.h4s6.09
Polar ICL3 R 221 - G.h4s6 D 315 G.h4s6.09
Hydrophobic ICL3 R 221 - G.h4s6 D 315 G.h4s6.09
Hydrophobic TM6 I 226 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 I 226 6x33 G.H5 L 353 G.H5.25
Aromatic ICL2 Y 138 - G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 Y 138 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 Y 138 - G.hns1 R 32 G.hns1.03
Ionic TM4 K 142 4x40 G.HN E 25 G.HN.49
Polar TM4 K 142 4x40 G.HN E 25 G.HN.49
Hydrophobic TM4 K 142 4x40 G.HN E 25 G.HN.49
Van-der-waals TM4 K 142 4x40 G.HN E 25 G.HN.49
Polar ICL2 Y 138 - G.H5 N 347 G.H5.19
Hydrophobic ICL2 Y 138 - G.H5 N 347 G.H5.19
Van-der-waals ICL2 Y 138 - G.H5 N 347 G.H5.19
Hydrophobic TM5 S 218 5x68 G.H5 I 344 G.H5.16
Van-der-waals ICL2 Y 138 - G.H5 I 343 G.H5.15
Hydrophobic H8 G 295 8x47 G.H5 F 354 G.H5.26
Van-der-waals H8 G 295 8x47 G.H5 F 354 G.H5.26
Aromatic TM6 H 222 6x29 G.H5 F 354 G.H5.26
Hydrophobic TM6 H 222 6x29 G.H5 F 354 G.H5.26
Van-der-waals TM6 H 222 6x29 G.H5 F 354 G.H5.26
Polar ICL3 Q 219 - G.S6 Y 320 G.S6.02
Van-der-waals ICL3 Q 219 - G.S6 Y 320 G.S6.02
Hydrophobic TM3 R 129 3x50 G.H5 L 353 G.H5.25
Hydrophobic TM3 I 133 3x54 G.H5 I 344 G.H5.16
Polar H8 T 296 8x48 G.H5 F 354 G.H5.26
Van-der-waals H8 T 296 8x48 G.H5 F 354 G.H5.26
Polar TM6 R 225 6x32 G.H5 F 354 G.H5.26
Van-der-waals TM6 R 225 6x32 G.H5 F 354 G.H5.26
Polar TM3 R 129 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM3 R 129 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM6 I 226 6x33 G.H5 F 354 G.H5.26
Van-der-waals TM6 I 226 6x33 G.H5 F 354 G.H5.26
Hydrophobic TM3 A 132 3x53 G.H5 C 351 G.H5.23
Hydrophobic TM3 I 133 3x54 G.H5 N 347 G.H5.19
Hydrophobic ICL2 M 136 - G.H5 I 343 G.H5.15
Polar TM4 V 141 4x39 G.HN E 28 G.HN.52
Hydrophobic TM4 V 141 4x39 G.HN E 28 G.HN.52
Van-der-waals TM4 V 141 4x39 G.HN E 28 G.HN.52
Polar TM3 A 132 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 A 132 3x53 G.H5 N 347 G.H5.19
Van-der-waals ICL3 Q 219 - G.H5 K 345 G.H5.17
Hydrophobic TM5 V 215 5x65 G.H5 L 348 G.H5.20
Van-der-waals TM5 V 215 5x65 G.H5 L 348 G.H5.20
Hydrophobic TM6 I 226 6x33 G.H5 L 348 G.H5.20
Van-der-waals TM6 I 226 6x33 G.H5 L 348 G.H5.20
Polar H8 G 295 8x47 G.H5 G 352 G.H5.24
Hydrophobic TM5 I 211 5x61 G.H5 L 353 G.H5.25
Ionic H8 K 297 8x49 G.H5 D 350 G.H5.22
Polar H8 K 297 8x49 G.H5 D 350 G.H5.22
Hydrophobic TM5 I 214 5x64 G.H5 I 344 G.H5.16
Hydrophobic ICL2 M 136 - G.H5 T 340 G.H5.12
Van-der-waals TM7 V 294 7x56 G.H5 G 352 G.H5.24
Hydrophobic TM3 I 133 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 I 133 3x54 G.H5 L 348 G.H5.20