EP4 receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

RESOLUTION

3.1 Å

REFERENCE

PUBLICATION DATE

Jan. 3, 2024

3D VIEW

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LIGAND INTERACTIONS

LIGANDS

PHYSIOLOGICAL LIGANDS

PGE1, PGI2, thromboxane A2, PGF, PGD2, PGE2



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

, , , GPCR
Interaction cut-off: strict (stick), loose (line)

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Interaction
type
GPCR
Segment

AA

Number
Generic
number

segment

AA

Number
Generic
number
Polar ICL2 Y 127 34x53 G.H5 C 351 G.H5.23
Hydrophobic ICL2 Y 127 34x53 G.H5 C 351 G.H5.23
Van-der-waals ICL2 Y 127 34x53 G.H5 C 351 G.H5.23
Hydrophobic ICL2 Y 127 34x53 G.H5 N 347 G.H5.19
Van-der-waals ICL2 Y 127 34x53 G.H5 N 347 G.H5.19
Hydrophobic TM6 M 270 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 M 270 6x33 G.H5 L 353 G.H5.25
Polar TM3 A 120 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 A 120 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL1 T 52 - G.H5 D 350 G.H5.22
Van-der-waals ICL1 T 52 - G.H5 D 350 G.H5.22
Polar ICL2 Y 127 34x53 G.H5 D 350 G.H5.22
Hydrophobic ICL2 Y 127 34x53 G.H5 D 350 G.H5.22
Van-der-waals ICL2 Y 127 34x53 G.H5 D 350 G.H5.22
Hydrophobic TM2 F 54 2x39 G.H5 G 352 G.H5.24
Van-der-waals TM2 F 54 2x39 G.H5 G 352 G.H5.24
Hydrophobic TM3 A 120 3x53 G.H5 C 351 G.H5.23
Hydrophobic TM3 I 121 3x54 G.H5 I 344 G.H5.16
Polar ICL2 A 124 34x50 G.H5 N 347 G.H5.19
Van-der-waals ICL2 A 124 34x50 G.H5 N 347 G.H5.19
Hydrophobic ICL2 Y 125 34x51 G.H5 I 343 G.H5.15
Hydrophobic TM5 M 213 5x68 G.H5 I 344 G.H5.16
Polar ICL2 Y 125 34x51 G.H5 T 340 G.H5.12
Hydrophobic ICL2 Y 125 34x51 G.H5 T 340 G.H5.12
Polar ICL2 S 128 34x54 G.H5 N 347 G.H5.19
Hydrophobic ICL2 S 128 34x54 G.H5 N 347 G.H5.19
Hydrophobic ICL2 A 124 34x50 G.H5 I 344 G.H5.16
Hydrophobic ICL2 Y 125 34x51 G.H5 F 336 G.H5.08
Van-der-waals ICL2 Y 125 34x51 G.H5 F 336 G.H5.08
Hydrophobic ICL2 Y 125 34x51 G.S3 L 194 G.S3.01
Aromatic ICL2 H 129 34x55 G.hns1 R 32 G.hns1.03
Hydrophobic ICL2 H 129 34x55 G.hns1 R 32 G.hns1.03
Polar TM2 T 53 2x38 G.H5 D 350 G.H5.22
Van-der-waals TM2 T 53 2x38 G.H5 D 350 G.H5.22
Hydrophobic TM6 M 270 6x33 G.H5 F 354 G.H5.26
Van-der-waals TM6 M 270 6x33 G.H5 F 354 G.H5.26
Hydrophobic ICL1 T 52 - G.H5 K 349 G.H5.21
Van-der-waals ICL1 T 52 - G.H5 K 349 G.H5.21
Hydrophobic TM3 I 121 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 I 121 3x54 G.H5 L 348 G.H5.20
Polar TM2 F 54 2x39 G.H5 D 350 G.H5.22
Hydrophobic TM3 A 120 3x53 G.H5 L 348 G.H5.20
Polar TM3 R 117 3x50 G.H5 G 352 G.H5.24
Hydrophobic TM3 R 117 3x50 G.H5 C 351 G.H5.23
Hydrophobic TM2 F 54 2x39 G.H5 C 351 G.H5.23
Van-der-waals TM2 F 54 2x39 G.H5 C 351 G.H5.23
Hydrophobic TM3 E 116 3x49 G.H5 C 351 G.H5.23
Van-der-waals TM3 E 116 3x49 G.H5 C 351 G.H5.23