GPR4 - Gs

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Danio rerio

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8ZFJ

RESOLUTION

3.1 Å

REFERENCE

PUBLICATION DATE

Aug. 7, 2024

3D VIEW

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LIGAND INTERACTIONS

0 interactions (Click to see)

LIGANDS

Apo (no ligand) - Apo (no ligand)

PHYSIOLOGICAL LIGANDS

No physiological ligands available



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Ionic TM3 R 123 3x50 G.H5 E 375 G.H5.24
Polar ICL3 S 221 - G.H5 R 368 G.H5.17
Hydrophobic ICL3 S 221 - G.H5 R 368 G.H5.17
Van-der-waals ICL3 S 221 - G.H5 R 368 G.H5.17
Hydrophobic ICL2 L 131 - G.H5 F 359 G.H5.08
Van-der-waals ICL2 L 131 - G.H5 F 359 G.H5.08
Hydrophobic ICL2 L 131 - G.S3 V 210 G.S3.01
Hydrophobic ICL2 P 130 - G.H5 Q 367 G.H5.16
Hydrophobic TM2 L 60 2x39 G.H5 Y 374 G.H5.23
Hydrophobic ICL3 T 222 - G.H5 L 371 G.H5.20
Hydrophobic ICL3 S 221 - G.S6 Y 343 G.S6.02
Van-der-waals ICL3 S 221 - G.S6 Y 343 G.S6.02
Polar TM3 A 128 3x55 G.H5 R 363 G.H5.12
Van-der-waals TM3 A 128 3x55 G.H5 R 363 G.H5.12
Hydrophobic TM5 Y 209 5x58 G.H5 L 376 G.H5.25
Polar TM3 V 127 3x54 G.H5 Q 367 G.H5.16
Hydrophobic TM3 V 127 3x54 G.H5 Q 367 G.H5.16
Van-der-waals TM3 V 127 3x54 G.H5 Q 367 G.H5.16
Hydrophobic ICL2 L 131 - G.H5 R 363 G.H5.12
Polar TM1 Q 53 1x60 G.H5 E 375 G.H5.24
Hydrophobic ICL2 P 130 - G.H5 R 363 G.H5.12
Van-der-waals ICL2 P 130 - G.H5 R 363 G.H5.12
Polar TM4 R 138 4x38 G.HN Q 28 G.HN.52
Polar TM5 N 219 5x68 G.H5 D 364 G.H5.13
Hydrophobic TM7 N 298 7x57 G.H5 E 375 G.H5.24
Hydrophobic TM6 I 230 6x33 G.H5 L 376 G.H5.25
Van-der-waals TM6 I 230 6x33 G.H5 L 376 G.H5.25
Polar TM3 R 123 3x50 G.H5 Y 374 G.H5.23
Hydrophobic TM3 R 123 3x50 G.H5 Y 374 G.H5.23
Van-der-waals TM3 R 123 3x50 G.H5 Y 374 G.H5.23
Hydrophobic TM6 I 230 6x33 G.H5 L 377 G.H5.26
Van-der-waals TM6 I 230 6x33 G.H5 L 377 G.H5.26
Hydrophobic TM2 L 60 2x39 G.H5 E 375 G.H5.24
Hydrophobic TM3 D 122 3x49 G.H5 Y 374 G.H5.23
Van-der-waals TM7 Y 294 7x53 G.H5 E 375 G.H5.24
Polar ICL1 N 58 - G.H5 Q 373 G.H5.22
Hydrophobic ICL1 N 58 - G.H5 Q 373 G.H5.22
Van-der-waals ICL1 N 58 - G.H5 Q 373 G.H5.22
Hydrophobic ICL3 S 221 - G.h4s6 Y 341 G.h4s6.20
Hydrophobic TM6 L 233 6x36 G.H5 L 376 G.H5.25
Hydrophobic ICL2 L 131 - G.H5 I 366 G.H5.15
Hydrophobic TM3 V 127 3x54 G.H5 L 376 G.H5.25
Polar ICL2 R 137 - G.H5 H 370 G.H5.19
Aromatic ICL2 R 137 - G.H5 H 370 G.H5.19
Hydrophobic ICL2 R 137 - G.H5 H 370 G.H5.19
Van-der-waals ICL2 R 137 - G.H5 H 370 G.H5.19
Hydrophobic ICL3 V 220 - G.h4s6 Y 341 G.h4s6.20
Polar ICL3 V 220 - G.H5 D 364 G.H5.13
Hydrophobic ICL3 V 220 - G.H5 D 364 G.H5.13
Van-der-waals ICL3 V 220 - G.H5 D 364 G.H5.13
Polar ICL2 A 134 - G.hns1 R 31 G.hns1.02
Hydrophobic ICL2 A 134 - G.hns1 R 31 G.hns1.02
Van-der-waals ICL2 A 134 - G.hns1 R 31 G.hns1.02
Polar TM3 A 126 3x53 G.H5 H 370 G.H5.19
Hydrophobic TM3 A 126 3x53 G.H5 H 370 G.H5.19
Van-der-waals TM3 A 126 3x53 G.H5 H 370 G.H5.19
Polar ICL2 R 137 - G.H5 Y 374 G.H5.23
Hydrophobic ICL2 R 137 - G.H5 Y 374 G.H5.23
Van-der-waals ICL2 R 137 - G.H5 Y 374 G.H5.23
Ionic TM6 E 226 6x29 G.H5 R 368 G.H5.17
Polar TM6 E 226 6x29 G.H5 R 368 G.H5.17
Hydrophobic TM6 E 226 6x29 G.H5 R 368 G.H5.17
Van-der-waals TM6 E 226 6x29 G.H5 R 368 G.H5.17
Hydrophobic TM5 V 216 5x65 G.H5 L 371 G.H5.20
Van-der-waals TM5 V 216 5x65 G.H5 L 371 G.H5.20
Hydrophobic TM2 L 64 2x43 G.H5 E 375 G.H5.24
Hydrophobic TM3 V 127 3x54 G.H5 L 371 G.H5.20
Hydrophobic ICL2 L 131 - G.S1 H 34 G.S1.02
Van-der-waals ICL2 L 131 - G.S1 H 34 G.S1.02
Hydrophobic TM3 A 126 3x53 G.H5 Y 374 G.H5.23
Van-der-waals TM3 A 126 3x53 G.H5 Y 374 G.H5.23
Hydrophobic TM3 R 123 3x50 G.H5 L 376 G.H5.25
Polar TM5 N 219 5x68 G.H5 Q 367 G.H5.16
Hydrophobic TM5 N 219 5x68 G.H5 Q 367 G.H5.16
Van-der-waals TM5 N 219 5x68 G.H5 Q 367 G.H5.16
Polar TM2 E 59 2x38 G.H5 Y 374 G.H5.23
Hydrophobic TM2 E 59 2x38 G.H5 Y 374 G.H5.23
Van-der-waals TM2 E 59 2x38 G.H5 Y 374 G.H5.23
Hydrophobic ICL3 T 222 - G.H5 R 368 G.H5.17
Van-der-waals ICL3 T 222 - G.H5 R 368 G.H5.17
Hydrophobic TM6 I 230 6x33 G.H5 L 371 G.H5.20
Hydrophobic ICL3 V 220 - G.S6 Y 343 G.S6.02
Hydrophobic TM5 I 212 5x61 G.H5 L 376 G.H5.25
Polar TM5 A 215 5x64 G.H5 Q 367 G.H5.16
Hydrophobic TM5 A 215 5x64 G.H5 Q 367 G.H5.16
Hydrophobic TM6 E 226 6x29 G.H5 L 377 G.H5.26
Hydrophobic ICL2 P 130 - G.H5 I 366 G.H5.15
Hydrophobic ICL2 P 130 - G.H5 H 370 G.H5.19
Van-der-waals ICL2 P 130 - G.H5 H 370 G.H5.19
Hydrophobic TM3 V 127 3x54 G.H5 H 370 G.H5.19