Refined structure (6GDG) of Gs - A2A receptor

GPCR CLASS (FAMILY)

A (Rhodopsin)

SPECIES

Homo sapiens

G PROTEIN FAMILY

Gs

DATE

2024-02-01

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Structure
GPCR
Gα
Gβ
Gγ
Model
AlphaFold2-Multimer

REFINED G PROTEIN RESIDUES

Residue backbones - 225 from structure (57.1%), 169 from model (42.9%)
Residue sidechains - 183 from structure (46.4%), 211 from model (53.6%)
Segment Seq.num. AA CGN# Backbone from Sidechain from
G.HN 1 M G.HN.01 AF2 AF2
G.HN 2 G G.HN.02 AF2 AF2
G.HN 3 C G.HN.09 AF2 AF2
G.HN 4 L G.HN.10 AF2 AF2
G.HN 5 G G.HN.11 AF2 AF2
G.HN 6 N G.HN.22 AF2 AF2
G.HN 7 S G.HN.23 AF2 AF2
G.HN 8 K G.HN.24 AF2 AF2
G.HN 9 T G.HN.26 AF2 AF2
G.HN 10 E G.HN.27 AF2 AF2
G.HN 11 D G.HN.28 AF2 AF2
G.HN 12 Q G.HN.29 Structure AF2
G.HN 13 R G.HN.30 Structure AF2
G.HN 14 N G.HN.31 Structure Structure
G.HN 15 E G.HN.32 Structure AF2
G.HN 16 E G.HN.33 Structure Structure
G.HN 17 K G.HN.34 Structure Structure
G.HN 18 A G.HN.35 Structure Structure
G.HN 19 Q G.HN.36 Structure Structure
G.HN 20 R G.HN.37 Structure AF2
G.HN 21 E G.HN.38 Structure Structure
G.HN 22 A G.HN.39 Structure Structure
G.HN 23 N G.HN.40 Structure Structure
G.HN 24 K G.HN.41 Structure AF2
G.HN 25 K G.HN.42 Structure Structure
G.HN 26 I G.HN.43 Structure AF2
G.HN 27 E G.HN.44 Structure Structure
G.HN 28 K G.HN.45 Structure AF2
G.HN 29 Q G.HN.46 Structure Structure
G.HN 30 L G.HN.47 Structure Structure
G.HN 31 Q G.HN.48 Structure Structure
G.HN 32 K G.HN.49 Structure AF2
G.HN 33 D G.HN.50 Structure AF2
G.HN 34 K G.HN.51 Structure Structure
G.HN 35 Q G.HN.52 Structure AF2
G.HN 36 V G.HN.53 Structure Structure
G.hns1 37 Y G.hns1.01 Structure Structure
G.hns1 38 R G.hns1.02 Structure AF2
G.hns1 39 A G.hns1.03 Structure Structure
G.S1 40 T G.S1.01 Structure Structure
G.S1 41 H G.S1.02 Structure Structure
G.S1 42 R G.S1.03 Structure Structure
G.S1 43 L G.S1.04 Structure Structure
G.S1 44 L G.S1.05 Structure Structure
G.S1 45 L G.S1.06 Structure Structure
G.S1 46 L G.S1.07 Structure Structure
G.s1h1 47 G G.s1h1.01 Structure Structure
G.s1h1 48 A G.s1h1.02 Structure Structure
G.s1h1 49 G G.s1h1.03 Structure AF2
G.s1h1 50 E G.s1h1.04 Structure AF2
G.s1h1 51 S G.s1h1.05 Structure Structure
G.s1h1 52 G G.s1h1.06 Structure Structure
G.H1 53 K G.H1.01 Structure Structure
G.H1 54 S G.H1.02 Structure Structure
G.H1 55 T G.H1.03 Structure Structure
G.H1 56 I G.H1.04 Structure Structure
G.H1 57 V G.H1.05 Structure Structure
G.H1 58 K G.H1.06 AF2 AF2
G.H1 59 Q G.H1.07 AF2 AF2
G.H1 60 M G.H1.08 AF2 AF2
G.H1 61 R G.H1.09 AF2 AF2
G.H1 62 I G.H1.10 AF2 AF2
G.H1 63 L G.H1.11 AF2 AF2
G.H1 64 H G.H1.12 AF2 AF2
G.h1ha 65 V G.h1ha.01 AF2 AF2
G.h1ha 66 N G.h1ha.02 AF2 AF2
G.h1ha 67 G G.h1ha.03 AF2 AF2
G.h1ha 68 F G.h1ha.04 AF2 AF2
G.h1ha 69 N G.h1ha.05 AF2 AF2
G.h1ha 70 G G.h1ha.06 AF2 AF2
G.h1ha 71 E G.h1ha.07 AF2 AF2
G.h1ha 72 G G.h1ha.08 AF2 AF2
G.h1ha 73 G G.h1ha.09 AF2 AF2
G.h1ha 74 E G.h1ha.10 AF2 AF2
G.h1ha 75 E G.h1ha.11 AF2 AF2
G.h1ha 76 D G.h1ha.12 AF2 AF2
G.h1ha 77 P G.h1ha.13 AF2 AF2
G.h1ha 78 Q G.h1ha.14 AF2 AF2
G.h1ha 79 A G.h1ha.15 AF2 AF2
G.h1ha 80 A G.h1ha.16 AF2 AF2
G.h1ha 81 R G.h1ha.17 AF2 AF2
G.h1ha 82 S G.h1ha.18 AF2 AF2
G.h1ha 83 N G.h1ha.19 AF2 AF2
G.h1ha 84 S G.h1ha.20 AF2 AF2
H.HA 85 D H.HA.01 AF2 AF2
H.HA 86 G H.HA.02 AF2 AF2
H.HA 87 E H.HA.03 AF2 AF2
H.HA 88 K H.HA.04 AF2 AF2
H.HA 89 A H.HA.05 AF2 AF2
H.HA 90 T H.HA.06 AF2 AF2
H.HA 91 K H.HA.07 AF2 AF2
H.HA 92 V H.HA.08 AF2 AF2
H.HA 93 Q H.HA.09 AF2 AF2
H.HA 94 D H.HA.10 AF2 AF2
H.HA 95 I H.HA.11 AF2 AF2
H.HA 96 K H.HA.12 AF2 AF2
H.HA 97 N H.HA.13 AF2 AF2
H.HA 98 N H.HA.14 AF2 AF2
H.HA 99 L H.HA.15 AF2 AF2
H.HA 100 K H.HA.16 AF2 AF2
H.HA 101 E H.HA.17 AF2 AF2
H.HA 102 A H.HA.18 AF2 AF2
H.HA 103 I H.HA.19 AF2 AF2
H.HA 104 E H.HA.20 AF2 AF2
H.HA 105 T H.HA.21 AF2 AF2
H.HA 106 I H.HA.22 AF2 AF2
H.HA 107 V H.HA.23 AF2 AF2
H.HA 108 A H.HA.24 AF2 AF2
H.HA 109 A H.HA.25 AF2 AF2
H.HA 110 M H.HA.26 AF2 AF2
H.HA 111 S H.HA.27 AF2 AF2
H.HA 112 N H.HA.28 AF2 AF2
H.HA 113 L H.HA.29 AF2 AF2
H.hahb 114 V H.hahb.01 AF2 AF2
H.hahb 115 P H.hahb.02 AF2 AF2
H.hahb 116 P H.hahb.03 AF2 AF2
H.hahb 117 V H.hahb.04 AF2 AF2
H.hahb 118 E H.hahb.05 AF2 AF2
H.hahb 119 L H.hahb.06 AF2 AF2
H.hahb 120 A H.hahb.07 AF2 AF2
H.hahb 121 N H.hahb.08 AF2 AF2
H.hahb 122 P H.hahb.09 AF2 AF2
H.HB 123 E H.HB.01 AF2 AF2
H.HB 124 N H.HB.02 AF2 AF2
H.HB 125 Q H.HB.03 AF2 AF2
H.HB 126 F H.HB.04 AF2 AF2
H.HB 127 R H.HB.05 AF2 AF2
H.HB 128 V H.HB.06 AF2 AF2
H.HB 129 D H.HB.07 AF2 AF2
H.HB 130 Y H.HB.08 AF2 AF2
H.HB 131 I H.HB.09 AF2 AF2
H.HB 132 L H.HB.10 AF2 AF2
H.HB 133 S H.HB.11 AF2 AF2
H.HB 134 V H.HB.12 AF2 AF2
H.HB 135 M H.HB.13 AF2 AF2
H.HB 136 N H.HB.14 AF2 AF2
H.hbhc 137 V H.hbhc.01 AF2 AF2
H.hbhc 138 P H.hbhc.02 AF2 AF2
H.hbhc 139 D H.hbhc.03 AF2 AF2
H.hbhc 140 F H.hbhc.04 AF2 AF2
H.hbhc 141 D H.hbhc.13 AF2 AF2
H.hbhc 142 F H.hbhc.14 AF2 AF2
H.hbhc 143 P H.hbhc.15 AF2 AF2
H.HC 144 P H.HC.01 AF2 AF2
H.HC 145 E H.HC.02 AF2 AF2
H.HC 146 F H.HC.03 AF2 AF2
H.HC 147 Y H.HC.04 AF2 AF2
H.HC 148 E H.HC.05 AF2 AF2
H.HC 149 H H.HC.06 AF2 AF2
H.HC 150 A H.HC.07 AF2 AF2
H.HC 151 K H.HC.08 AF2 AF2
H.HC 152 A H.HC.09 AF2 AF2
H.HC 153 L H.HC.10 AF2 AF2
H.HC 154 W H.HC.11 AF2 AF2
H.HC 155 E H.HC.12 AF2 AF2
H.hchd 156 D H.hchd.01 AF2 AF2
H.HD 157 E H.HD.01 AF2 AF2
H.HD 158 G H.HD.02 AF2 AF2
H.HD 159 V H.HD.03 AF2 AF2
H.HD 160 R H.HD.04 AF2 AF2
H.HD 161 A H.HD.05 AF2 AF2
H.HD 162 C H.HD.06 AF2 AF2
H.HD 163 Y H.HD.07 AF2 AF2
H.HD 164 E H.HD.08 AF2 AF2
H.HD 165 R H.HD.09 AF2 AF2
H.HD 166 S H.HD.10 AF2 AF2
H.HD 167 N H.HD.11 AF2 AF2
H.HD 168 E H.HD.12 AF2 AF2
H.hdhe 169 Y H.hdhe.01 AF2 AF2
H.hdhe 170 Q H.hdhe.02 AF2 AF2
H.hdhe 171 L H.hdhe.03 AF2 AF2
H.hdhe 172 I H.hdhe.04 AF2 AF2
H.hdhe 173 D H.hdhe.05 AF2 AF2
H.HE 174 C H.HE.01 AF2 AF2
H.HE 175 A H.HE.02 AF2 AF2
H.HE 176 Q H.HE.03 AF2 AF2
H.HE 177 Y H.HE.04 AF2 AF2
H.HE 178 F H.HE.05 AF2 AF2
H.HE 179 L H.HE.06 AF2 AF2
H.HE 180 D H.HE.07 AF2 AF2
H.HE 181 K H.HE.08 AF2 AF2
H.HE 182 I H.HE.09 AF2 AF2
H.HE 183 D H.HE.10 AF2 AF2
H.HE 184 V H.HE.11 AF2 AF2
H.HE 185 I H.HE.12 AF2 AF2
H.HE 186 K H.HE.13 AF2 AF2
H.hehf 187 Q H.hehf.01 AF2 AF2
H.hehf 188 A H.hehf.02 AF2 AF2
H.hehf 189 D H.hehf.03 AF2 AF2
H.hehf 190 Y H.hehf.04 AF2 AF2
H.hehf 191 V H.hehf.05 AF2 AF2
H.hehf 192 P H.hehf.06 AF2 AF2
H.hehf 193 S H.hehf.07 AF2 AF2
H.HF 194 D H.HF.01 AF2 AF2
H.HF 195 Q H.HF.02 AF2 AF2
H.HF 196 D H.HF.03 AF2 AF2
H.HF 197 L H.HF.04 AF2 AF2
H.HF 198 L H.HF.05 AF2 AF2
H.HF 199 R H.HF.06 AF2 AF2
G.hfs2 200 C G.hfs2.01 AF2 AF2
G.hfs2 201 R G.hfs2.02 AF2 AF2
G.hfs2 202 V G.hfs2.03 AF2 AF2
G.hfs2 203 L G.hfs2.04 AF2 AF2
G.hfs2 204 T G.hfs2.05 AF2 AF2
G.hfs2 205 S G.hfs2.06 AF2 AF2
G.hfs2 206 G G.hfs2.07 Structure Structure
G.S2 207 I G.S2.01 Structure AF2
G.S2 208 F G.S2.02 Structure Structure
G.S2 209 E G.S2.03 Structure Structure
G.S2 210 T G.S2.04 Structure Structure
G.S2 211 K G.S2.05 Structure AF2
G.S2 212 F G.S2.06 Structure Structure
G.S2 213 Q G.S2.07 Structure AF2
G.S2 214 V G.S2.08 Structure Structure
G.s2s3 215 D G.s2s3.01 Structure Structure
G.s2s3 216 K G.s2s3.02 Structure AF2
G.S3 217 V G.S3.01 Structure Structure
G.S3 218 N G.S3.02 Structure Structure
G.S3 219 F G.S3.03 Structure Structure
G.S3 220 H G.S3.04 Structure Structure
G.S3 221 M G.S3.05 Structure Structure
G.S3 222 F G.S3.06 Structure Structure
G.S3 223 D G.S3.07 Structure Structure
G.S3 224 V G.S3.08 Structure Structure
G.s3h2 225 G G.s3h2.01 Structure Structure
G.s3h2 226 G G.s3h2.02 Structure Structure
G.s3h2 227 Q G.s3h2.03 Structure AF2
G.H2 228 R G.H2.01 Structure Structure
G.H2 229 D G.H2.02 Structure Structure
G.H2 230 E G.H2.03 Structure Structure
G.H2 231 R G.H2.04 Structure Structure
G.H2 232 R G.H2.05 Structure Structure
G.H2 233 K G.H2.06 Structure Structure
G.H2 234 W G.H2.07 Structure Structure
G.H2 235 I G.H2.08 Structure Structure
G.H2 236 Q G.H2.09 Structure Structure
G.H2 237 C G.H2.10 Structure Structure
G.h2s4 238 F G.h2s4.01 Structure Structure
G.h2s4 239 N G.h2s4.02 Structure Structure
G.h2s4 240 D G.h2s4.03 Structure Structure
G.h2s4 241 V G.h2s4.04 Structure Structure
G.h2s4 242 T G.h2s4.05 Structure Structure
G.S4 243 A G.S4.01 Structure Structure
G.S4 244 I G.S4.02 Structure Structure
G.S4 245 I G.S4.03 Structure Structure
G.S4 246 F G.S4.04 Structure Structure
G.S4 247 V G.S4.05 Structure Structure
G.S4 248 V G.S4.06 Structure Structure
G.S4 249 A G.S4.07 Structure AF2
G.s4h3 250 S G.s4h3.01 Structure Structure
G.s4h3 251 S G.s4h3.02 Structure Structure
G.s4h3 252 S G.s4h3.03 Structure AF2
G.s4h3 253 Y G.s4h3.04 Structure Structure
G.s4h3 254 N G.s4h3.05 Structure Structure
G.s4h3 255 M G.s4h3.06 AF2 AF2
G.s4h3 256 V G.s4h3.07 AF2 AF2
G.s4h3 257 I G.s4h3.08 AF2 AF2
G.s4h3 258 R G.s4h3.09 AF2 AF2
G.s4h3 259 E G.s4h3.10 AF2 AF2
G.s4h3 260 D G.s4h3.11 AF2 AF2
G.s4h3 261 N G.s4h3.12 AF2 AF2
G.s4h3 262 Q G.s4h3.13 AF2 AF2
G.s4h3 263 T G.s4h3.14 AF2 AF2
G.s4h3 264 N G.s4h3.15 AF2 AF2
G.H3 265 R G.H3.01 Structure Structure
G.H3 266 L G.H3.02 Structure Structure
G.H3 267 Q G.H3.03 Structure Structure
G.H3 268 E G.H3.04 Structure AF2
G.H3 269 A G.H3.05 Structure Structure
G.H3 270 L G.H3.06 Structure Structure
G.H3 271 N G.H3.07 Structure Structure
G.H3 272 L G.H3.08 Structure Structure
G.H3 273 F G.H3.09 Structure Structure
G.H3 274 K G.H3.10 Structure AF2
G.H3 275 S G.H3.11 Structure Structure
G.H3 276 I G.H3.12 Structure Structure
G.H3 277 W G.H3.13 Structure Structure
G.H3 278 N G.H3.14 Structure Structure
G.H3 279 N G.H3.15 Structure Structure
G.H3 280 R G.H3.16 Structure Structure
G.H3 281 W G.H3.17 Structure Structure
G.H3 282 L G.H3.18 Structure Structure
G.h3s5 283 R G.h3s5.01 Structure Structure
G.h3s5 284 T G.h3s5.02 Structure Structure
G.h3s5 285 I G.h3s5.03 Structure Structure
G.S5 286 S G.S5.01 Structure Structure
G.S5 287 V G.S5.02 Structure Structure
G.S5 288 I G.S5.03 Structure Structure
G.S5 289 L G.S5.04 Structure Structure
G.S5 290 F G.S5.05 Structure Structure
G.S5 291 L G.S5.06 Structure Structure
G.S5 292 N G.S5.07 Structure Structure
G.s5hg 293 K G.s5hg.01 Structure AF2
G.HG 294 Q G.HG.01 Structure Structure
G.HG 295 D G.HG.02 Structure Structure
G.HG 296 L G.HG.03 Structure Structure
G.HG 297 L G.HG.04 Structure Structure
G.HG 298 A G.HG.05 Structure Structure
G.HG 299 E G.HG.06 Structure AF2
G.HG 300 K G.HG.07 Structure AF2
G.HG 301 V G.HG.08 Structure Structure
G.HG 302 L G.HG.09 Structure Structure
G.HG 303 A G.HG.10 Structure Structure
G.HG 304 G G.HG.11 Structure Structure
G.HG 305 K G.HG.12 Structure AF2
G.HG 306 S G.HG.13 Structure Structure
G.HG 307 K G.HG.14 Structure AF2
G.HG 308 I G.HG.15 Structure Structure
G.HG 309 E G.HG.16 Structure AF2
G.HG 310 D G.HG.17 Structure Structure
G.hgh4 311 Y G.hgh4.01 Structure Structure
G.hgh4 312 F G.hgh4.02 Structure Structure
G.hgh4 313 P G.hgh4.03 Structure Structure
G.hgh4 314 E G.hgh4.04 Structure AF2
G.hgh4 315 F G.hgh4.05 Structure Structure
G.hgh4 316 A G.hgh4.06 Structure Structure
G.hgh4 317 R G.hgh4.07 Structure Structure
G.hgh4 318 Y G.hgh4.08 Structure Structure
G.hgh4 319 T G.hgh4.09 Structure Structure
G.hgh4 320 T G.hgh4.10 Structure Structure
G.hgh4 321 P G.hgh4.11 Structure Structure
G.hgh4 322 E G.hgh4.12 Structure AF2
G.hgh4 323 D G.hgh4.13 Structure AF2
G.hgh4 324 A G.hgh4.14 Structure Structure
G.hgh4 325 T G.hgh4.15 Structure Structure
G.hgh4 326 P G.hgh4.16 Structure Structure
G.hgh4 327 E G.hgh4.17 Structure Structure
G.hgh4 328 P G.hgh4.18 Structure Structure
G.hgh4 329 G G.hgh4.19 Structure Structure
G.hgh4 330 E G.hgh4.20 Structure AF2
G.hgh4 331 D G.hgh4.21 Structure Structure
G.H4 332 P G.H4.01 Structure Structure
G.H4 333 R G.H4.02 Structure Structure
G.H4 334 V G.H4.03 Structure Structure
G.H4 335 T G.H4.04 Structure Structure
G.H4 336 R G.H4.05 Structure AF2
G.H4 337 A G.H4.06 Structure Structure
G.H4 338 K G.H4.07 Structure Structure
G.H4 339 Y G.H4.08 Structure Structure
G.H4 340 F G.H4.10 Structure Structure
G.H4 341 I G.H4.11 Structure Structure
G.H4 342 R G.H4.12 Structure Structure
G.H4 343 D G.H4.13 Structure AF2
G.H4 344 E G.H4.14 Structure Structure
G.H4 345 F G.H4.15 Structure Structure
G.H4 346 L G.H4.16 Structure Structure
G.H4 347 R G.H4.17 Structure Structure
G.h4s6 348 I G.h4s6.01 Structure Structure
G.h4s6 349 S G.h4s6.02 Structure Structure
G.h4s6 350 T G.h4s6.03 Structure Structure
G.h4s6 351 A G.h4s6.04 Structure Structure
G.h4s6 352 S G.h4s6.08 Structure Structure
G.h4s6 353 G G.h4s6.09 Structure Structure
G.h4s6 354 D G.h4s6.10 Structure AF2
G.h4s6 355 G G.h4s6.11 Structure Structure
G.h4s6 356 R G.h4s6.12 Structure Structure
G.h4s6 357 H G.h4s6.13 Structure Structure
G.h4s6 358 Y G.h4s6.20 Structure Structure
G.S6 359 C G.S6.01 Structure Structure
G.S6 360 Y G.S6.02 Structure Structure
G.S6 361 P G.S6.03 Structure Structure
G.S6 362 H G.S6.04 Structure Structure
G.S6 363 F G.S6.05 Structure Structure
G.s6h5 364 T G.s6h5.01 Structure Structure
G.s6h5 365 C G.s6h5.02 Structure Structure
G.s6h5 366 A G.s6h5.03 Structure Structure
G.s6h5 367 V G.s6h5.04 Structure AF2
G.s6h5 368 D G.s6h5.05 Structure Structure
G.H5 369 T G.H5.01 Structure Structure
G.H5 370 E G.H5.02 Structure Structure
G.H5 371 N G.H5.03 Structure Structure
G.H5 372 I G.H5.04 Structure AF2
G.H5 373 R G.H5.05 Structure Structure
G.H5 374 R G.H5.06 Structure AF2
G.H5 375 V G.H5.07 Structure AF2
G.H5 376 F G.H5.08 Structure Structure
G.H5 377 N G.H5.09 Structure Structure
G.H5 378 D G.H5.10 Structure AF2
G.H5 379 C G.H5.11 Structure Structure
G.H5 380 R G.H5.12 Structure Structure
G.H5 381 D G.H5.13 Structure Structure
G.H5 382 I G.H5.14 Structure Structure
G.H5 383 I G.H5.15 Structure Structure
G.H5 384 Q G.H5.16 Structure Structure
G.H5 385 R G.H5.17 Structure AF2
G.H5 386 M G.H5.18 Structure AF2
G.H5 387 H G.H5.19 Structure Structure
G.H5 388 L G.H5.20 Structure Structure
G.H5 389 R G.H5.21 Structure Structure
G.H5 390 Q G.H5.22 Structure Structure
G.H5 391 Y G.H5.23 Structure Structure
G.H5 392 E G.H5.24 Structure Structure
G.H5 393 L G.H5.25 Structure Structure
G.H5 394 L G.H5.26 Structure Structure

REFINED GPCR RESIDUES

Residue backbones - 263 from structure (81.2%), 61 from model (18.8%)
Residue sidechains - 224 from structure (69.1%), 100 from model (30.9%)
Segment Seq.num. AA GPCRdb# Backbone from Sidechain from
TM1 1 M 1.27x27 AF2 AF2
TM1 2 P 1.28x28 AF2 AF2
TM1 3 I 1.29x29 AF2 AF2
TM1 4 M 1.30x30 AF2 AF2
TM1 5 G 1.31x31 AF2 AF2
TM1 6 S 1.32x32 AF2 AF2
TM1 7 S 1.33x33 AF2 AF2
TM1 8 V 1.34x34 Structure AF2
TM1 9 Y 1.35x35 Structure AF2
TM1 10 I 1.36x36 Structure Structure
TM1 11 T 1.37x37 Structure AF2
TM1 12 V 1.38x38 Structure AF2
TM1 13 E 1.39x39 Structure AF2
TM1 14 L 1.40x40 Structure Structure
TM1 15 A 1.41x41 Structure Structure
TM1 16 I 1.42x42 Structure AF2
TM1 17 A 1.43x43 Structure Structure
TM1 18 V 1.44x44 Structure AF2
TM1 19 L 1.45x45 Structure AF2
TM1 20 A 1.46x46 Structure Structure
TM1 21 I 1.47x47 Structure Structure
TM1 22 L 1.48x48 Structure AF2
TM1 23 G 1.49x49 Structure Structure
TM1 24 N 1.50x50 Structure AF2
TM1 25 V 1.51x51 Structure Structure
TM1 26 L 1.52x52 Structure Structure
TM1 27 V 1.53x53 Structure Structure
TM1 28 C 1.54x54 Structure Structure
TM1 29 W 1.55x55 Structure Structure
TM1 30 A 1.56x56 Structure Structure
TM1 31 V 1.57x57 Structure Structure
TM1 32 W 1.58x58 Structure Structure
TM1 33 L 1.59x59 Structure AF2
TM1 34 N 1.60x60 Structure Structure
ICL1 35 S 12.48x48 Structure Structure
ICL1 36 N 12.49x49 Structure Structure
ICL1 37 L 12.50x50 Structure Structure
ICL1 38 Q 12.51x51 Structure Structure
TM2 39 N 2.37x37 Structure AF2
TM2 40 V 2.38x38 Structure Structure
TM2 41 T 2.39x39 Structure Structure
TM2 42 N 2.40x40 Structure AF2
TM2 43 Y 2.41x41 Structure Structure
TM2 44 F 2.42x42 Structure Structure
TM2 45 V 2.43x43 Structure Structure
TM2 46 V 2.44x44 Structure Structure
TM2 47 S 2.45x45 Structure Structure
TM2 48 L 2.46x46 Structure Structure
TM2 49 A 2.47x47 Structure Structure
TM2 50 A 2.48x48 Structure Structure
TM2 51 A 2.49x49 Structure Structure
TM2 52 D 2.50x50 Structure AF2
TM2 53 I 2.51x51 Structure Structure
TM2 54 A 2.52x52 Structure Structure
TM2 55 V 2.53x53 Structure Structure
TM2 56 G 2.54x54 Structure Structure
TM2 57 V 2.55x55 Structure Structure
TM2 58 L 2.56x551 Structure Structure
TM2 59 A 2.57x56 Structure Structure
TM2 60 I 2.58x57 Structure Structure
TM2 61 P 2.59x58 Structure Structure
TM2 62 F 2.60x59 Structure Structure
TM2 63 A 2.61x60 Structure Structure
TM2 64 I 2.62x61 Structure Structure
TM2 65 T 2.63x62 Structure Structure
TM2 66 I 2.64x63 Structure Structure
TM2 67 S 2.65x64 Structure Structure
TM2 68 T 2.66x65 Structure Structure
TM2 69 G 2.67x66 Structure Structure
ECL1 70 F 23.50x50 Structure Structure
ECL1 71 C 23.51x51 Structure Structure
ECL1 72 A 23.52x52 Structure Structure
TM3 73 A 3.21x21 Structure Structure
TM3 74 C 3.22x22 Structure Structure
TM3 75 H 3.23x23 Structure AF2
TM3 76 G 3.24x24 Structure Structure
TM3 77 C 3.25x25 Structure Structure
TM3 78 L 3.26x26 Structure Structure
TM3 79 F 3.27x27 Structure Structure
TM3 80 I 3.28x28 Structure Structure
TM3 81 A 3.29x29 Structure Structure
TM3 82 C 3.30x30 Structure Structure
TM3 83 F 3.31x31 Structure Structure
TM3 84 V 3.32x32 Structure Structure
TM3 85 L 3.33x33 Structure Structure
TM3 86 V 3.34x34 Structure Structure
TM3 87 L 3.35x35 Structure Structure
TM3 88 T 3.36x36 Structure Structure
TM3 89 Q 3.37x37 Structure Structure
TM3 90 S 3.38x38 Structure Structure
TM3 91 S 3.39x39 Structure Structure
TM3 92 I 3.40x40 Structure Structure
TM3 93 F 3.41x41 Structure Structure
TM3 94 S 3.42x42 Structure Structure
TM3 95 L 3.43x43 Structure Structure
TM3 96 L 3.44x44 Structure Structure
TM3 97 A 3.45x45 Structure Structure
TM3 98 I 3.46x46 Structure Structure
TM3 99 A 3.47x47 Structure Structure
TM3 100 I 3.48x48 Structure Structure
TM3 101 D 3.49x49 Structure Structure
TM3 102 R 3.50x50 Structure Structure
TM3 103 Y 3.51x51 Structure Structure
TM3 104 I 3.52x52 Structure Structure
TM3 105 A 3.53x53 Structure Structure
TM3 106 I 3.54x54 Structure Structure
TM3 107 R 3.55x55 Structure Structure
TM3 108 I 3.56x56 Structure Structure
ICL2 109 P 34.50x50 Structure Structure
ICL2 110 L 34.51x51 Structure Structure
ICL2 111 R 34.52x52 Structure Structure
ICL2 112 Y 34.53x53 Structure Structure
ICL2 113 N 34.54x54 Structure Structure
ICL2 114 G 34.55x55 Structure Structure
ICL2 115 L 34.56x56 Structure Structure
ICL2 116 V 34.57x57 Structure Structure
TM4 117 T 4.38x38 Structure AF2
TM4 118 G 4.39x39 Structure Structure
TM4 119 T 4.40x40 Structure Structure
TM4 120 R 4.41x41 Structure Structure
TM4 121 A 4.42x42 Structure Structure
TM4 122 K 4.43x43 Structure AF2
TM4 123 G 4.44x44 Structure Structure
TM4 124 I 4.45x45 Structure Structure
TM4 125 I 4.46x46 Structure Structure
TM4 126 A 4.47x47 Structure Structure
TM4 127 I 4.48x48 Structure Structure
TM4 128 C 4.49x49 Structure Structure
TM4 129 W 4.50x50 Structure Structure
TM4 130 V 4.51x51 Structure Structure
TM4 131 L 4.52x52 Structure Structure
TM4 132 S 4.53x53 Structure Structure
TM4 133 F 4.54x54 Structure Structure
TM4 134 A 4.55x55 Structure Structure
TM4 135 I 4.56x56 Structure Structure
TM4 136 G 4.57x57 Structure Structure
TM4 137 L 4.58x58 Structure Structure
TM4 138 T 4.59x59 Structure Structure
TM4 139 P 4.60x60 Structure Structure
TM4 140 M 4.61x61 Structure Structure
TM4 141 L 4.62x62 Structure AF2
TM4 142 G 4.63x63 Structure Structure
ECL2 143 W - Structure Structure
ECL2 144 N - Structure AF2
ECL2 145 N - Structure Structure
ECL2 146 C - Structure Structure
ECL2 147 G - AF2 AF2
ECL2 148 Q - AF2 AF2
ECL2 149 P - AF2 AF2
ECL2 150 K - AF2 AF2
ECL2 151 E - AF2 AF2
ECL2 152 G - AF2 AF2
ECL2 153 K - AF2 AF2
ECL2 154 N - AF2 AF2
ECL2 155 H - AF2 AF2
ECL2 156 S - AF2 AF2
ECL2 157 Q - AF2 AF2
ECL2 158 G - AF2 AF2
ECL2 159 C - AF2 AF2
ECL2 160 G - AF2 AF2
ECL2 161 E - AF2 AF2
ECL2 162 G - AF2 AF2
ECL2 163 Q - AF2 AF2
ECL2 164 V - Structure AF2
ECL2 165 A - Structure Structure
ECL2 166 C 45.50x50 Structure Structure
ECL2 167 L 45.51x51 Structure AF2
ECL2 168 F 45.52x52 Structure AF2
ECL2 169 E - Structure AF2
ECL2 170 D - Structure AF2
ECL2 171 V - Structure Structure
ECL2 172 V - Structure Structure
TM5 173 P 5.34x36 Structure Structure
TM5 174 M 5.35x37 Structure Structure
TM5 175 N 5.36x38 Structure AF2
TM5 176 Y 5.37x39 Structure Structure
TM5 177 M 5.38x40 Structure Structure
TM5 178 V 5.39x41 Structure Structure
TM5 179 Y 5.40x411 Structure Structure
TM5 180 F 5.41x42 Structure Structure
TM5 181 N 5.42x43 Structure Structure
TM5 182 F 5.43x44 Structure Structure
TM5 183 F 5.44x45 Structure AF2
TM5 184 A 5.45x46 Structure Structure
TM5 185 C 5.46x461 Structure Structure
TM5 186 V 5.47x47 Structure Structure
TM5 187 L 5.48x48 Structure Structure
TM5 188 V 5.49x49 Structure Structure
TM5 189 P 5.50x50 Structure Structure
TM5 190 L 5.51x51 Structure Structure
TM5 191 L 5.52x52 Structure Structure
TM5 192 L 5.53x53 Structure Structure
TM5 193 M 5.54x54 Structure Structure
TM5 194 L 5.55x55 Structure Structure
TM5 195 G 5.56x56 Structure Structure
TM5 196 V 5.57x57 Structure Structure
TM5 197 Y 5.58x58 Structure Structure
TM5 198 L 5.59x59 Structure Structure
TM5 199 R 5.60x60 Structure Structure
TM5 200 I 5.61x61 Structure Structure
TM5 201 F 5.62x62 Structure Structure
TM5 202 L 5.63x63 Structure Structure
TM5 203 A 5.64x64 Structure Structure
TM5 204 A 5.65x65 Structure Structure
TM5 205 R 5.66x66 Structure Structure
TM5 206 R 5.67x67 Structure Structure
TM5 207 Q 5.68x68 Structure Structure
TM5 208 L 5.69x69 Structure Structure
TM5 209 K 5.70x70 Structure Structure
TM5 210 Q 5.71x71 Structure Structure
TM5 211 M 5.72x72 Structure Structure
TM5 212 E 5.73x73 AF2 AF2
TM5 213 S 5.74x74 AF2 AF2
ICL3 214 Q - AF2 AF2
ICL3 215 P - AF2 AF2
ICL3 216 L - AF2 AF2
ICL3 217 P - AF2 AF2
ICL3 218 G - AF2 AF2
TM6 219 E 6.21x21 AF2 AF2
TM6 220 R 6.22x22 AF2 AF2
TM6 221 A 6.23x23 AF2 AF2
TM6 222 R 6.24x24 AF2 AF2
TM6 223 S 6.25x25 AF2 AF2
TM6 224 T 6.26x26 Structure Structure
TM6 225 L 6.27x27 Structure AF2
TM6 226 Q 6.28x28 Structure AF2
TM6 227 K 6.29x29 Structure AF2
TM6 228 E 6.30x30 Structure AF2
TM6 229 V 6.31x31 Structure Structure
TM6 230 H 6.32x32 Structure Structure
TM6 231 A 6.33x33 Structure Structure
TM6 232 A 6.34x34 Structure Structure
TM6 233 K 6.35x35 Structure AF2
TM6 234 S 6.36x36 Structure Structure
TM6 235 L 6.37x37 Structure Structure
TM6 236 A 6.38x38 Structure Structure
TM6 237 I 6.39x39 Structure Structure
TM6 238 I 6.40x40 Structure Structure
TM6 239 V 6.41x41 Structure Structure
TM6 240 G 6.42x42 Structure Structure
TM6 241 L 6.43x43 Structure Structure
TM6 242 F 6.44x44 Structure Structure
TM6 243 A 6.45x45 Structure Structure
TM6 244 L 6.46x46 Structure Structure
TM6 245 C 6.47x47 Structure Structure
TM6 246 W 6.48x48 Structure Structure
TM6 247 L 6.49x49 Structure Structure
TM6 248 P 6.50x50 Structure Structure
TM6 249 L 6.51x51 Structure Structure
TM6 250 H 6.52x52 Structure Structure
TM6 251 I 6.53x53 Structure Structure
TM6 252 I 6.54x54 Structure Structure
TM6 253 N 6.55x55 Structure Structure
TM6 254 C 6.56x56 Structure Structure
TM6 255 F 6.57x57 Structure Structure
TM6 256 T 6.58x58 Structure Structure
TM6 257 F 6.59x59 Structure Structure
TM6 258 F 6.60x60 Structure AF2
TM6 259 C 6.61x61 Structure Structure
ECL3 260 P - Structure Structure
ECL3 261 D - Structure AF2
ECL3 262 C - Structure Structure
ECL3 263 S - Structure Structure
ECL3 264 H - Structure Structure
ECL3 265 A - Structure Structure
TM7 266 P 7.31x30 Structure Structure
TM7 267 L 7.32x31 Structure Structure
TM7 268 W 7.33x32 Structure AF2
TM7 269 L 7.34x33 Structure Structure
TM7 270 M 7.35x34 Structure Structure
TM7 271 Y 7.36x35 Structure Structure
TM7 272 L 7.37x36 Structure Structure
TM7 273 A 7.38x37 Structure Structure
TM7 274 I 7.39x38 Structure Structure
TM7 275 V 7.40x39 Structure Structure
TM7 276 L 7.41x40 Structure Structure
TM7 277 S 7.42x41 Structure Structure
TM7 278 H 7.43x42 Structure Structure
TM7 279 T 7.44x43 Structure Structure
TM7 280 N 7.45x45 Structure Structure
TM7 281 S 7.46x46 Structure Structure
TM7 282 V 7.47x47 Structure Structure
TM7 283 V 7.48x48 Structure Structure
TM7 284 N 7.49x49 Structure Structure
TM7 285 P 7.50x50 Structure Structure
TM7 286 F 7.51x51 Structure Structure
TM7 287 I 7.52x52 Structure Structure
TM7 288 Y 7.53x53 Structure Structure
TM7 289 A 7.54x54 Structure Structure
TM7 290 Y 7.55x55 Structure Structure
TM7 291 R 7.56x56 Structure Structure
H8 292 I 8.47x47 Structure AF2
H8 293 R 8.48x48 Structure AF2
H8 294 E 8.49x49 Structure AF2
H8 295 F 8.50x50 Structure Structure
H8 296 R 8.51x51 Structure Structure
H8 297 Q 8.52x52 Structure AF2
H8 298 T 8.53x53 Structure Structure
H8 299 F 8.54x54 Structure AF2
H8 300 R 8.55x55 AF2 AF2
H8 301 K 8.56x56 AF2 AF2
H8 302 I 8.57x57 AF2 AF2
H8 303 I 8.58x58 AF2 AF2
H8 304 R 8.59x59 AF2 AF2
H8 305 S 8.60x60 AF2 AF2
H8 306 H 8.61x61 AF2 AF2
H8 307 V 8.62x62 AF2 AF2
H8 308 L 8.63x63 AF2 AF2
H8 309 R 8.64x64 AF2 AF2
H8 310 Q 8.65x65 AF2 AF2
H8 311 Q 8.66x66 AF2 AF2
H8 312 E 8.67x67 AF2 AF2
H8 313 P 8.68x68 AF2 AF2
H8 314 F 8.69x69 AF2 AF2
C-term 315 K - AF2 AF2
C-term 316 A - AF2 AF2
C-term 317 A - AF2 AF2
C-term 318 G - AF2 AF2
C-term 319 T - AF2 AF2
C-term 320 S - AF2 AF2
C-term 321 A - AF2 AF2
C-term 322 R - AF2 AF2
C-term 323 V - AF2 AF2
C-term 324 L - AF2 AF2



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction cut-off:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar TM5 Q 207 5x68 G.H5 D 381 G.H5.13
Hydrophobic TM5 Q 207 5x68 G.H5 D 381 G.H5.13
Van-der-waals TM5 Q 207 5x68 G.H5 D 381 G.H5.13
Hydrophobic ICL2 G 114 34x55 G.hns1 A 39 G.hns1.03
Hydrophobic ICL2 P 109 34x50 G.H5 I 383 G.H5.15
Van-der-waals ICL2 P 109 34x50 G.H5 I 383 G.H5.15
Hydrophobic TM6 A 231 6x33 G.H5 L 393 G.H5.25
Van-der-waals TM6 A 231 6x33 G.H5 L 393 G.H5.25
Hydrophobic TM5 M 211 5x72 G.H5 R 385 G.H5.17
Aromatic TM3 R 102 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 102 3x50 G.H5 Y 391 G.H5.23
Van-der-waals TM3 R 102 3x50 G.H5 Y 391 G.H5.23
Hydrophobic ICL3 P 217 - G.h4s6 A 351 G.h4s6.04
Hydrophobic ICL2 L 110 34x51 G.H5 C 379 G.H5.11
Hydrophobic TM5 M 211 5x72 G.h4s6 Y 358 G.h4s6.20
Van-der-waals TM5 M 211 5x72 G.h4s6 Y 358 G.h4s6.20
Hydrophobic ICL2 R 111 34x52 G.S3 V 217 G.S3.01
Van-der-waals ICL2 R 111 34x52 G.S3 V 217 G.S3.01
Polar ICL2 L 110 34x51 G.S1 H 41 G.S1.02
Hydrophobic ICL2 L 110 34x51 G.S1 H 41 G.S1.02
Van-der-waals ICL2 L 110 34x51 G.S1 H 41 G.S1.02
Hydrophobic ICL3 Q 214 - G.H4 L 346 G.H4.16
Polar C-term V 323 - G.h3s5 R 283 G.h3s5.01
Hydrophobic C-term V 323 - G.h3s5 R 283 G.h3s5.01
Van-der-waals C-term V 323 - G.h3s5 R 283 G.h3s5.01
Aromatic ICL2 Y 112 34x53 G.H5 H 387 G.H5.19
Hydrophobic ICL2 Y 112 34x53 G.H5 H 387 G.H5.19
Polar ICL3 Q 214 - G.S6 C 359 G.S6.01
Hydrophobic ICL3 Q 214 - G.S6 C 359 G.S6.01
Van-der-waals ICL3 Q 214 - G.S6 C 359 G.S6.01
Polar TM5 Q 210 5x71 G.H5 D 381 G.H5.13
Hydrophobic C-term L 324 - G.H3 R 280 G.H3.16
Van-der-waals C-term L 324 - G.H3 R 280 G.H3.16
Van-der-waals TM3 R 107 3x55 G.H5 R 380 G.H5.12
Polar TM7 R 291 7x56 G.H5 E 392 G.H5.24
Van-der-waals TM7 R 291 7x56 G.H5 E 392 G.H5.24
Polar H8 R 293 8x48 G.H5 Q 390 G.H5.22
Polar C-term L 324 - G.h3s5 R 283 G.h3s5.01
Hydrophobic C-term L 324 - G.h3s5 R 283 G.h3s5.01
Van-der-waals C-term L 324 - G.h3s5 R 283 G.h3s5.01
Hydrophobic ICL3 L 216 - G.h4s6 S 349 G.h4s6.02
Van-der-waals ICL3 L 216 - G.h4s6 S 349 G.h4s6.02
Polar TM3 A 105 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM3 A 105 3x53 G.H5 H 387 G.H5.19
Van-der-waals TM3 A 105 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM5 M 211 5x72 G.H5 L 394 G.H5.26
Van-der-waals TM5 M 211 5x72 G.H5 L 394 G.H5.26
Hydrophobic TM3 I 106 3x54 G.H5 H 387 G.H5.19
Van-der-waals TM3 I 106 3x54 G.H5 H 387 G.H5.19
Hydrophobic TM3 I 106 3x54 G.H5 Y 391 G.H5.23
Hydrophobic ICL3 Q 214 - G.S6 P 361 G.S6.03
Van-der-waals ICL3 Q 214 - G.S6 P 361 G.S6.03
Hydrophobic TM5 Q 207 5x68 G.H5 L 388 G.H5.20
Polar ICL3 P 217 - G.h4s6 T 350 G.h4s6.03
Hydrophobic ICL3 P 217 - G.h4s6 T 350 G.h4s6.03
Van-der-waals ICL3 P 217 - G.h4s6 T 350 G.h4s6.03
Polar TM5 Q 207 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM5 Q 207 5x68 G.H5 Q 384 G.H5.16
Van-der-waals TM5 Q 207 5x68 G.H5 Q 384 G.H5.16
Hydrophobic TM6 E 219 6x21 G.h4s6 G 353 G.h4s6.09
Hydrophobic ICL2 L 110 34x51 G.H5 R 380 G.H5.12
Hydrophobic TM5 I 200 5x61 G.H5 L 393 G.H5.25
Van-der-waals TM5 I 200 5x61 G.H5 L 393 G.H5.25
Hydrophobic H8 I 292 8x47 G.H5 Q 390 G.H5.22
Van-der-waals H8 I 292 8x47 G.H5 Q 390 G.H5.22
Hydrophobic ICL3 L 216 - G.h4s6 Y 358 G.h4s6.20
Hydrophobic C-term V 323 - G.h4s6 R 356 G.h4s6.12
Van-der-waals C-term V 323 - G.h4s6 R 356 G.h4s6.12
Hydrophobic ICL2 L 110 34x51 G.H5 F 376 G.H5.08
Van-der-waals ICL2 L 110 34x51 G.H5 F 376 G.H5.08
Hydrophobic TM5 A 204 5x65 G.H5 L 388 G.H5.20
Hydrophobic TM3 I 106 3x54 G.H5 L 388 G.H5.20
Van-der-waals TM3 I 106 3x54 G.H5 L 388 G.H5.20
Hydrophobic ICL2 L 110 34x51 G.S3 V 217 G.S3.01
Polar TM3 I 106 3x54 G.H5 Q 384 G.H5.16
Hydrophobic TM3 I 106 3x54 G.H5 Q 384 G.H5.16
Van-der-waals TM3 I 106 3x54 G.H5 Q 384 G.H5.16
Hydrophobic ICL2 N 113 34x54 G.hns1 A 39 G.hns1.03
Polar ICL2 N 113 34x54 G.hns1 R 38 G.hns1.02
Hydrophobic ICL2 N 113 34x54 G.hns1 R 38 G.hns1.02
Van-der-waals ICL2 N 113 34x54 G.hns1 R 38 G.hns1.02
Polar ICL3 P 215 - G.h4s6 T 350 G.h4s6.03
Hydrophobic ICL3 P 215 - G.h4s6 T 350 G.h4s6.03
Van-der-waals ICL3 P 215 - G.h4s6 T 350 G.h4s6.03
Hydrophobic ICL2 R 111 34x52 G.s2s3 K 216 G.s2s3.02
Van-der-waals ICL2 R 111 34x52 G.s2s3 K 216 G.s2s3.02
Hydrophobic ICL2 L 110 34x51 G.H5 I 383 G.H5.15
Hydrophobic ICL2 P 109 34x50 G.H5 R 380 G.H5.12
Hydrophobic ICL3 Q 214 - G.h4s6 T 350 G.h4s6.03
Hydrophobic TM5 Q 207 5x68 G.H5 L 394 G.H5.26
Hydrophobic H8 R 293 8x48 G.H5 R 389 G.H5.21
Van-der-waals H8 R 293 8x48 G.H5 R 389 G.H5.21
Hydrophobic ICL2 P 109 34x50 G.H5 Q 384 G.H5.16
Van-der-waals ICL2 P 109 34x50 G.H5 Q 384 G.H5.16
Hydrophobic TM5 A 203 5x64 G.H5 L 388 G.H5.20
Polar TM6 S 234 6x36 G.H5 E 392 G.H5.24
Polar TM5 Q 207 5x68 G.H5 R 385 G.H5.17
Hydrophobic TM5 Q 207 5x68 G.H5 R 385 G.H5.17
Van-der-waals TM5 Q 207 5x68 G.H5 R 385 G.H5.17
Polar H8 R 293 8x48 G.H5 E 392 G.H5.24
Hydrophobic H8 R 293 8x48 G.H5 E 392 G.H5.24
Van-der-waals H8 R 293 8x48 G.H5 E 392 G.H5.24
Hydrophobic TM6 L 235 6x37 G.H5 L 393 G.H5.25
Van-der-waals TM6 L 235 6x37 G.H5 L 393 G.H5.25
Hydrophobic ICL3 L 216 - G.h4s6 T 350 G.h4s6.03
Van-der-waals ICL3 L 216 - G.h4s6 T 350 G.h4s6.03
Hydrophobic TM6 S 234 6x36 G.H5 L 393 G.H5.25
Van-der-waals TM6 S 234 6x36 G.H5 L 393 G.H5.25