Inhibitory activity against 5-hydroxytryptamine 1E receptor subtypeInhibitory activity against 5-hydroxytryptamine 1E receptor subtype

Compound was evaluated for the affinity at 5-hydroxytryptamine 1E receptorCompound was evaluated for the affinity at 5-hydroxytryptamine 1E receptor

Compound was evaluated for the affinity at 5-hydroxytryptamine 1E receptorCompound was evaluated for the affinity at 5-hydroxytryptamine 1E receptor

Compound was evaluated for the affinity at 5-hydroxytryptamine 1E receptorCompound was evaluated for the affinity at 5-hydroxytryptamine 1E receptor

Binding affinity to 5HT1E receptorBinding affinity to 5HT1E receptor

Inhibitory activity against 5-hydroxytryptamine 1E receptor subtypeInhibitory activity against 5-hydroxytryptamine 1E receptor subtype

Displacement of [3H]5HT from 5-HT1E receptor (unknown origin)Displacement of [3H]5HT from 5-HT1E receptor (unknown origin)

Displacement of [3H]5HT from 5-HT1E receptor (unknown origin)Displacement of [3H]5HT from 5-HT1E receptor (unknown origin)

Binding affinity to serotonin 5-HT1E receptor (unknown origin) by PDSP assayBinding affinity to serotonin 5-HT1E receptor (unknown origin) by PDSP assay

Binding affinity to serotonin 5-HT1E receptor (unknown origin) by PDSP assayBinding affinity to serotonin 5-HT1E receptor (unknown origin) by PDSP assay

Binding affinity to serotonin 5-HT1E receptor (unknown origin) by PDSP assayBinding affinity to serotonin 5-HT1E receptor (unknown origin) by PDSP assay

The compound was tested for the binding affinity towards human cloned 5-hydroxytryptamine 1E receptor in CHO cells, using [3H]5-HT as radioligandThe compound was tested for the binding affinity towards human cloned 5-hydroxytryptamine 1E receptor in CHO cells, using [3H]5-HT as radioligand

Binding affinity to human 5-HT1E receptorBinding affinity to human 5-HT1E receptor

GPCRScan assay: inhibition of 5-HT1EGPCRScan assay: inhibition of 5-HT1E

Displacement of [3H]5HT from 5-HT1E receptor (unknown origin)Displacement of [3H]5HT from 5-HT1E receptor (unknown origin)

Displacement of [3H]5HT from 5-HT1E receptor (unknown origin)Displacement of [3H]5HT from 5-HT1E receptor (unknown origin)

Displacement of [3H]-5-HT from human 5-HT1E receptor expressed in stable HEK cells after 90 mins by microbeta scintillation counting methodDisplacement of [3H]-5-HT from human 5-HT1E receptor expressed in stable HEK cells after 90 mins by microbeta scintillation counting method

PDSP Secondary Binding target: HTR1E - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.PDSP Secondary Binding target: HTR1E - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.

PDSP Secondary Binding target: HTR1E - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.PDSP Secondary Binding target: HTR1E - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.

PDSP Secondary Binding target: HTR1E - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.PDSP Secondary Binding target: HTR1E - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.

Displacement of [3H]LSD from human 5-HT1E receptor expressed in HEK cell membranes after 1.5 hrs by microbeta scintillation counting methodDisplacement of [3H]LSD from human 5-HT1E receptor expressed in HEK cell membranes after 1.5 hrs by microbeta scintillation counting method

Binding affinity towards 5-hydroxytryptamine 1E receptorBinding affinity towards 5-hydroxytryptamine 1E receptor

Displacement of [3H]5-HT from human 5-HT1E receptor expressed in HEK cells after 90 mins by microbeta scintillation counting analysisDisplacement of [3H]5-HT from human 5-HT1E receptor expressed in HEK cells after 90 mins by microbeta scintillation counting analysis

Binding affinity to 5HT1E receptor (unknown origin) assessed as inhibition constantBinding affinity to 5HT1E receptor (unknown origin) assessed as inhibition constant

PDSP Secondary Binding target: HTR1E - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.PDSP Secondary Binding target: HTR1E - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.